Restriction map for
pF1KB6555
(Circular) (Six-base) MAPSORT of: pF1KB6555.seq Check: 2261 from: 1 to: 4229
pF1KB6555 4229 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 692 692
Size: 4229
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 488 2054 2486 3233 3501 488
Size: 1566 432 747 268 1216
Fragments arranged by size:
1566 1216 747 432 268
AflIII A'CryG_T
Cuts at: 1581 2685 3662 1581
Size: 1104 977 2148
Fragments arranged by size:
2148 1104 977
AgeI A'CCGG_T
Cuts at: 2617 2617
Size: 4229
AleI CACnn'nnGTG
Cuts at: 919 919
Size: 4229
AlwNI CAG_nnn'CTG
Cuts at: 1534 3101 1534
Size: 1567 2662
ApaLI G'TGCA_C
Cuts at: 2999 2999
Size: 4229
ApoI r'AATT_y
Cuts at: 1233 3582 3838 1233
Size: 2349 256 1624
Fragments arranged by size:
2349 1624 256
AseI AT'TA_AT
Cuts at: 20 409 422 20
Size: 389 13 3827
Fragments arranged by size:
3827 389 13
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 316 1249 316
Size: 933 3296
BamHI G'GATC_C
Cuts at: 360 1254 360
Size: 894 3335
BanI G'GyrC_C
Cuts at: 1245 1659 1907 1942 1245
Size: 414 248 35 3532
Fragments arranged by size:
3532 414 248 35
BanII G_rGCy'C
Cuts at: 1013 1106 1243 2273 1013
Size: 93 137 1030 2969
Fragments arranged by size:
2969 1030 137 93
BbeI G_GCGC'C
Cuts at: 1663 1911 1663
Size: 248 3981
BbsI GAAGACnn'nnnn_
Cuts at: 1154 1154
Size: 4229
BbvCI CC'TCA_GC
Cuts at: 289 539 289
Size: 250 3979
BciVI GTATCCnnnnn_n'
Cuts at: 2127 2888 4229 2127
Size: 761 1341 2127
Fragments arranged by size:
2127 1341 761
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 1034 1854 1947 3003 1034
Size: 820 93 1056 2260
Fragments arranged by size:
2260 1056 820 93
BmrI ACTGGGnnnn_n'
Cuts at: 543 966 1441 1522 1857 4001 543
Size: 423 475 81 335 2144 771
Fragments arranged by size:
2144 771 475 423 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 535 610 766 970 1036 352
Size: 183 75 156 204 66 3545
Fragments arranged by size:
3545 204 183 156 75 66
Bpu10I CC'TnA_GC
Cuts at: 289 539 289
Size: 250 3979
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1405 2284 2776 3074 1405
Size: 879 492 298 2560
Fragments arranged by size:
2560 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 1582 1582
Size: 4229
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 1660 1908 2610 4106 1660
Size: 248 702 1496 1783
Fragments arranged by size:
1783 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1418 1939 2617 2891 3038 3449 1418
Size: 521 678 274 147 411 2198
Fragments arranged by size:
2198 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 349 274
Size: 75 4154
BseYI C'CCAG_C
Cuts at: 1654 2412 2989 3485 1654
Size: 758 577 496 2398
Fragments arranged by size:
2398 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1021
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3025 104
Size: 1667 46 1208 1308
Fragments arranged by size:
1667 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1106 1243 2021 2211 3003 1106
Size: 137 778 190 792 2332
Fragments arranged by size:
2332 792 778 190 137
Bsp1286I G_dGCh'C
Cuts at: 1013 1034 1106 1243 1854 1947 2021 2211 2273
Size: 21 72 137 611 93 74 190 62
Cuts at: 2273 3003 1013
Size: 730 2239
Fragments arranged by size:
2239 730 611 190 137 93 74 72
62 21
BspEI T'CCGG_A
Cuts at: 3449 3449
Size: 4229
BspHI T'CATG_A
Cuts at: 3405 3405
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 692 1279 1493 1795 2176 290
Size: 402 587 214 302 381 2343
Fragments arranged by size:
2343 587 402 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2521 2575 4059 4224 50
Size: 2471 54 1484 165 55
Fragments arranged by size:
2471 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 2227 2408 2617 2227
Size: 181 209 3839
Fragments arranged by size:
3839 209 181
BsrGI T'GTAC_A
Cuts at: 1190 1190
Size: 4229
BssSI C'ACGA_G
Cuts at: 2500 2858 2500
Size: 358 3871
BstAPI GCAn_nnn'nTGC
Cuts at: 576 1606 576
Size: 1030 3199
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstEII G'GTnAC_C
Cuts at: 543 543
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 666 750 666
Size: 84 4145
BstYI r'GATC_y
Cuts at: 1 360 1254 1721 1744 2079 2325 3337 3423
Size: 359 894 467 23 335 246 1012 86
Cuts at: 3423 3435 3781 1
Size: 12 346 449
Fragments arranged by size:
1012 894 467 449 359 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 1138 107
Size: 1031 3198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2179 2360 2523 2179
Size: 181 163 3885
Fragments arranged by size:
3885 181 163
BtsI GCAGTG_nn'
Cuts at: 381 1472 381
Size: 1091 3138
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DraIII CAC_nnn'GTG
Cuts at: 376 376
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 1935 2793 1935
Size: 858 3371
EaeI y'GGCC_r
Cuts at: 110 671 1141 1814 1988 2379 2406 3751 110
Size: 561 470 673 174 391 27 1345 588
Fragments arranged by size:
1345 673 588 561 470 391 174 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 1204 2252 2462 1204
Size: 1048 210 2971
Fragments arranged by size:
2971 1048 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2437 2747 2893 4039 2437
Size: 310 146 1146 2627
Fragments arranged by size:
2627 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1104 1241 1104
Size: 137 4092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 488 535 610 766 970 1036 2054 2486
Size: 136 47 75 156 204 66 1018 432
Cuts at: 2486 3233 3501 352
Size: 747 268 1080
Fragments arranged by size:
1080 1018 747 432 268 204 156 136
75 66 47
EcoO109I rG'GnC_Cy
Cuts at: 212 1368 212
Size: 1156 3073
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2248 2248
Size: 4229
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 1663 1911 2933 3912 1663
Size: 248 1022 979 1980
Fragments arranged by size:
1980 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 352 384 431 463 761 793 1156 1188 2071
Size: 32 47 32 298 32 363 32 883
Cuts at: 2071 2101 2103 2133 352
Size: 30 2 30 2448
Fragments arranged by size:
2448 883 363 298 47 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1158 1268 1158
Size: 110 4119
HindIII A'AGCT_T
Cuts at: 385 499 385
Size: 114 4115
KasI G'GCGC_C
Cuts at: 1659 1907 1659
Size: 248 3981
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 417 1103 2900 3084 417
Size: 686 1797 184 1562
Fragments arranged by size:
1797 1562 686 184
MscI TGG'CCA
Cuts at: 112 673 1990 112
Size: 561 1317 2351
Fragments arranged by size:
2351 1317 561
MslI CAynn'nnrTG
Cuts at: 748 845 919 2218 2345 748
Size: 97 74 1299 127 2632
Fragments arranged by size:
2632 1299 127 97 74
MspA1I CmG'CkG
Cuts at: 1140 1340 1654 2014 3027 3272 1140
Size: 200 314 360 1013 245 2097
Fragments arranged by size:
2097 1013 360 314 245 200
NaeI GCC'GGC
Cuts at: 2410 2410
Size: 4229
NarI GG'CG_CC
Cuts at: 1660 1908 1660
Size: 248 3981
NcoI C'CATG_G
Cuts at: 107 107
Size: 4229
NgoMIV G'CCGG_C
Cuts at: 2408 2408
Size: 4229
NspI r_CATG'y
Cuts at: 1282 1782 2689 1282
Size: 500 907 2822
Fragments arranged by size:
2822 907 500
PciI A'CATG_T
Cuts at: 2685 2685
Size: 4229
PfoI T'CCnGG_A
Cuts at: 587 832 1013 587
Size: 245 181 3803
Fragments arranged by size:
3803 245 181
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3368 3400 3368
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 212 212
Size: 4229
PstI C_TGCA'G
Cuts at: 257 497 581 1276 1961 257
Size: 240 84 695 685 2525
Fragments arranged by size:
2525 695 685 240 84
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 1654 2014 1654
Size: 360 3869
RsrII CG'GwC_CG
Cuts at: 2424 2424
Size: 4229
SacI G_AGCT'C
Cuts at: 1106 1243 1106
Size: 137 4092
SacII CC_GC'GG
Cuts at: 1141 1141
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SanDI GG'GwC_CC
Cuts at: 212 212
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 2252 2462 2252
Size: 210 4019
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SexAI A'CCwGG_T
Cuts at: 992 992
Size: 4229
SfcI C'TryA_G
Cuts at: 33 253 493 577 1272 1957 2950 3141 33
Size: 220 240 84 695 685 993 191 1121
Fragments arranged by size:
1121 993 695 685 240 220 191 84
SfoI GGC'GCC
Cuts at: 1661 1909 1661
Size: 248 3981
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 1251 1251
Size: 4229
SmlI C'TyrA_G
Cuts at: 1384 2299 2791 3053 1384
Size: 915 492 262 2560
Fragments arranged by size:
2560 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StyI C'CwwG_G
Cuts at: 107 236 1363 1717 107
Size: 129 1127 354 2619
Fragments arranged by size:
2619 1127 354 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 562 1812 1945 2404 2613 562
Size: 1250 133 459 209 2178
Fragments arranged by size:
2178 1250 459 209 133
TatI w'GTAC_w
Cuts at: 1190 3744 1190
Size: 2554 1675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 1153 2026 1153
Size: 873 3356
XbaI T'CTAG_A
Cuts at: 1260 1260
Size: 4229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 666 979 1724 666
Size: 313 745 3171
Fragments arranged by size:
3171 745 313
XmaI C'CCGG_G
Cuts at: 1249 1249
Size: 4229
XmnI GAAnn'nnTTC
Cuts at: 502 502
Size: 4229
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspOMI PspXI PsrI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI ZraI