Restriction map for
pF1KB6561
(Circular) (Six-base) MAPSORT of: pF1KB6561.seq Check: 5650 from: 1 to: 4235
pF1KB6561 4235 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2060 2492 3239 3507 2060
Size: 432 747 268 2788
Fragments arranged by size:
2788 747 432 268
AflII C'TTAA_G
Cuts at: 916 916
Size: 4235
AflIII A'CryG_T
Cuts at: 1587 2691 3668 1587
Size: 1104 977 2154
Fragments arranged by size:
2154 1104 977
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 829 829
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 416 1540 3107 416
Size: 1124 1567 1544
Fragments arranged by size:
1567 1544 1124
ApaLI G'TGCA_C
Cuts at: 1201 3005 1201
Size: 1804 2431
ApoI r'AATT_y
Cuts at: 555 1239 3588 3844 555
Size: 684 2349 256 946
Fragments arranged by size:
2349 946 684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 1255 1255
Size: 4235
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 359 598 1251 1665 1913 1948 359
Size: 239 653 414 248 35 2646
Fragments arranged by size:
2646 653 414 248 239 35
BanII G_rGCy'C
Cuts at: 1217 1249 2279 1217
Size: 32 1030 3173
Fragments arranged by size:
3173 1030 32
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BbsI GAAGACnn'nnnn_
Cuts at: 438 872 1142 438
Size: 434 270 3531
Fragments arranged by size:
3531 434 270
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 176 1755 176
Size: 1579 2656
BfrBI ATG'CAT
Cuts at: 694 694
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 1347 1347
Size: 4235
Bme1580I G_kGCm'C
Cuts at: 1205 1860 1953 3009 1205
Size: 655 93 1056 2431
Fragments arranged by size:
2431 1056 655 93
BmrI ACTGGGnnnn_n'
Cuts at: 1447 1528 1863 4007 1447
Size: 81 335 2144 1675
Fragments arranged by size:
2144 1675 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2602 2602
Size: 4235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 665 736 940 420
Size: 245 71 204 3715
Fragments arranged by size:
3715 245 204 71
Bpu10I CC'TnA_GC
Cuts at: 612 736 612
Size: 124 4111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 492 1411 2290 2782 3080 492
Size: 919 879 492 298 1647
Fragments arranged by size:
1647 919 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 376 438 3939 376
Size: 62 3501 672
Fragments arranged by size:
3501 672 62
BsaAI yAC'GTr
Cuts at: 1048 1588 1048
Size: 540 3695
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 1666 1914 2616 4112 1666
Size: 248 702 1496 1789
Fragments arranged by size:
1789 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1205 1424 1945 2623 2897 3044 3455 1205
Size: 219 521 678 274 147 411 1985
Fragments arranged by size:
1985 678 521 411 274 219 147
BseYI C'CCAG_C
Cuts at: 310 1660 2418 2995 3491 310
Size: 1350 758 577 496 1054
Fragments arranged by size:
1350 1054 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 245 1205 1249 2027 2217 3009 245
Size: 960 44 778 190 792 1471
Fragments arranged by size:
1471 960 792 778 190 44
BsmI GAATG_Cn'
Cuts at: 696 696
Size: 4235
Bsp1286I G_dGCh'C
Cuts at: 245 1205 1217 1249 1860 1953 2027 2217 2279
Size: 960 12 32 611 93 74 190 62
Cuts at: 2279 3009 245
Size: 730 1471
Fragments arranged by size:
1471 960 730 611 190 93 74 62
32 12
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 1104 3411 1104
Size: 2307 1928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 420 1285 1499 1801 2182 420
Size: 865 214 302 381 2473
Fragments arranged by size:
2473 865 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2527 2581 4065 4230 50
Size: 2477 54 1484 165 55
Fragments arranged by size:
2477 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 606 2147 606
Size: 1541 2694
BsrFI r'CCGG_y
Cuts at: 2233 2414 2623 2233
Size: 181 209 3845
Fragments arranged by size:
3845 209 181
BsrGI T'GTAC_A
Cuts at: 503 503
Size: 4235
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1612 1612
Size: 4235
BstBI TT'CG_AA
Cuts at: 2596 2596
Size: 4235
BstEII G'GTnAC_C
Cuts at: 729 729
Size: 4235
BstXI CCAn_nnnn'nTGG
Cuts at: 253 399 253
Size: 146 4089
BstYI r'GATC_y
Cuts at: 1 1260 1727 1750 2085 2331 3343 3429 3441
Size: 1259 467 23 335 246 1012 86 12
Cuts at: 3441 3787 1
Size: 346 449
Fragments arranged by size:
1259 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 193 1226 193
Size: 1033 3202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 613 2185 2366 2529 613
Size: 1572 181 163 2319
Fragments arranged by size:
2319 1572 181 163
BtsI GCAGTG_nn'
Cuts at: 179 1007 1478 179
Size: 828 471 2936
Fragments arranged by size:
2936 828 471
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 327 362 327
Size: 35 4200
DraI TTT'AAA
Cuts at: 1234 1234
Size: 4235
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 1820 1994 2385 2412 3757 1820
Size: 174 391 27 1345 2298
Fragments arranged by size:
2298 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 855 1148 2258 2468 855
Size: 293 1110 210 2622
Fragments arranged by size:
2622 1110 293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 2753 2899 4045 2443
Size: 310 146 1146 2633
Fragments arranged by size:
2633 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 420 665 736 940 2060 2492 3239 3507 420
Size: 245 71 204 1120 432 747 268 1148
Fragments arranged by size:
1148 1120 747 432 268 245 204 71
EcoO109I rG'GnC_Cy
Cuts at: 1374 1374
Size: 4235
EcoRI G'AATT_C
Cuts at: 555 1239 555
Size: 684 3551
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 774 2254 774
Size: 1480 2755
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 1669 1917 2939 3918 1669
Size: 248 1022 979 1986
Fragments arranged by size:
1986 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2077 2107 2109 2139 2077
Size: 30 2 30 4173
Fragments arranged by size:
4173 30 30 2
HincII GTy'rAC
Cuts at: 1274 1274
Size: 4235
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 914 2906 3090 914
Size: 1992 184 2059
Fragments arranged by size:
2059 1992 184
MscI TGG'CCA
Cuts at: 1996 1996
Size: 4235
MslI CAynn'nnrTG
Cuts at: 2224 2351 2224
Size: 127 4108
MspA1I CmG'CkG
Cuts at: 399 1188 1346 1660 2020 3033 3278 399
Size: 789 158 314 360 1013 245 1356
Fragments arranged by size:
1356 1013 789 360 314 245 158
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 4235
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NcoI C'CATG_G
Cuts at: 193 193
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 4235
NsiI A_TGCA'T
Cuts at: 696 696
Size: 4235
NspI r_CATG'y
Cuts at: 1288 1788 2695 1288
Size: 500 907 2828
Fragments arranged by size:
2828 907 500
PasI CC'CwG_GG
Cuts at: 1218 1218
Size: 4235
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PflMI CCAn_nnn'nTGG
Cuts at: 148 148
Size: 4235
PfoI T'CCnGG_A
Cuts at: 713 713
Size: 4235
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 817 849 3374 3406 817
Size: 32 2525 32 1646
Fragments arranged by size:
2525 1646 32 32
PstI C_TGCA'G
Cuts at: 431 1193 1282 1967 431
Size: 762 89 685 2699
Fragments arranged by size:
2699 762 685 89
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 399 1188 1660 2020 399
Size: 789 472 360 2614
Fragments arranged by size:
2614 789 472 360
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 1148 2258 2468 1148
Size: 1110 210 2915
Fragments arranged by size:
2915 1110 210
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 3752 3752
Size: 4235
SfcI C'TryA_G
Cuts at: 33 315 427 1189 1278 1963 2956 3147 33
Size: 282 112 762 89 685 993 191 1121
Fragments arranged by size:
1121 993 762 685 282 191 112 89
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 471 916 1390 2305 2797 3059 471
Size: 445 474 915 492 262 1647
Fragments arranged by size:
1647 915 492 474 445 262
SnaBI TAC'GTA
Cuts at: 1048 1048
Size: 4235
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
StuI AGG'CCT
Cuts at: 768 768
Size: 4235
StyI C'CwwG_G
Cuts at: 193 1369 1723 193
Size: 1176 354 2705
Fragments arranged by size:
2705 1176 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1026 1818 1951 2410 2619 1026
Size: 792 133 459 209 2642
Fragments arranged by size:
2642 792 459 209 133
TatI w'GTAC_w
Cuts at: 503 3750 503
Size: 3247 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4203
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 971 1730 971
Size: 759 3476
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmBI BssHII BstZ17I Bsu36I ClaI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SrfI SspI SwaI XhoI XmnI ZraI