Restriction map for
pF1KB6562
(Circular) (Six-base) MAPSORT of: pF1KB6562.seq Check: 8883 from: 1 to: 4310
pF1KB6562 4310 bp
With 182 enzymes: SgfI *
October 24, 2019 20:49 ..
AccI GT'mk_AC
Cuts at: 652 1348 652
Size: 696 3614
Acc65I G'GTAC_C
Cuts at: 1326 1326
Size: 4310
AclI AA'CG_TT
Cuts at: 4150 4150
Size: 4310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 388 597 2135 2567 3314 3582 259
Size: 129 209 1538 432 747 268 987
Fragments arranged by size:
1538 987 747 432 268 209 129
AflIII A'CryG_T
Cuts at: 1662 2766 3743 1662
Size: 1104 977 2229
Fragments arranged by size:
2229 1104 977
AgeI A'CCGG_T
Cuts at: 2698 2698
Size: 4310
AlwNI CAG_nnn'CTG
Cuts at: 1615 3182 1615
Size: 1567 2743
ApaLI G'TGCA_C
Cuts at: 605 3080 605
Size: 2475 1835
ApoI r'AATT_y
Cuts at: 246 503 716 1111 1314 3663 3919 246
Size: 257 213 395 203 2349 256 637
Fragments arranged by size:
2349 637 395 257 256 213 203
AseI AT'TA_AT
Cuts at: 20 20
Size: 4310
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
AvaI C'yCGr_G
Cuts at: 123 1119 1330 123
Size: 996 211 3103
Fragments arranged by size:
3103 996 211
BamHI G'GATC_C
Cuts at: 1335 1335
Size: 4310
BanI G'GyrC_C
Cuts at: 163 189 918 1326 1740 1988 2023 163
Size: 26 729 408 414 248 35 2450
Fragments arranged by size:
2450 729 414 408 248 35 26
BanII G_rGCy'C
Cuts at: 124 1324 2354 124
Size: 1200 1030 2080
Fragments arranged by size:
2080 1200 1030
BbeI G_GCGC'C
Cuts at: 193 1744 1992 193
Size: 1551 248 2511
Fragments arranged by size:
2511 1551 248
BciVI GTATCCnnnnn_n'
Cuts at: 2208 2969 4310 2208
Size: 761 1341 2208
Fragments arranged by size:
2208 1341 761
BclI T'GATC_A
Cuts at: 1050 1830 1050
Size: 780 3530
BglI GCCn_nnn'nGGC
Cuts at: 112 128 112
Size: 16 4294
BglII A'GATC_T
Cuts at: 371 1825 3862 371
Size: 1454 2037 819
Fragments arranged by size:
2037 1454 819
BlpI GC'TnA_GC
Cuts at: 1422 1422
Size: 4310
Bme1580I G_kGCm'C
Cuts at: 609 1935 2028 3084 609
Size: 1326 93 1056 1835
Fragments arranged by size:
1835 1326 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 399 1522 1603 1938 4082 399
Size: 1123 81 335 2144 627
Fragments arranged by size:
2144 1123 627 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 4310
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 999 999
Size: 4310
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2365 2857 3155 1486
Size: 879 492 298 2641
Fragments arranged by size:
2641 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4014 4014
Size: 4310
BsaAI yAC'GTr
Cuts at: 1663 1663
Size: 4310
BsaBI GATnn'nnATC
Cuts at: 6 1824 3861 6
Size: 1818 2037 455
Fragments arranged by size:
2037 1818 455
BsaHI Gr'CG_yC
Cuts at: 190 1741 1989 2691 4187 190
Size: 1551 248 702 1496 313
Fragments arranged by size:
1551 1496 702 313 248
BsaWI w'CCGG_w
Cuts at: 1499 2020 2698 2972 3119 3530 1499
Size: 521 678 274 147 411 2279
Fragments arranged by size:
2279 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 452 452
Size: 4310
BseYI C'CCAG_C
Cuts at: 1282 1735 2493 3070 3566 1282
Size: 453 758 577 496 2026
Fragments arranged by size:
2026 758 577 496 453
BsiEI CG_ry'CG
Cuts at: 104 206 1852 1898 3106 104
Size: 102 1646 46 1208 1308
Fragments arranged by size:
1646 1308 1208 102 46
BsiHKAI G_wGCw'C
Cuts at: 609 1324 2102 2292 3084 609
Size: 715 778 190 792 1835
Fragments arranged by size:
1835 792 778 715 190
Bsp1286I G_dGCh'C
Cuts at: 124 609 1324 1935 2028 2102 2292 2354 3084
Size: 485 715 611 93 74 190 62 730
Cuts at: 3084 124
Size: 1350
Fragments arranged by size:
1350 730 715 611 485 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3530 3530
Size: 4310
BspHI T'CATG_A
Cuts at: 3486 3486
Size: 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1360 1574 1876 2257 1360
Size: 214 302 381 3413
Fragments arranged by size:
3413 381 302 214
BsrBI CCG'CTC
Cuts at: 50 128 968 2602 2656 4140 4305 50
Size: 78 840 1634 54 1484 165 55
Fragments arranged by size:
1634 1484 840 165 78 55 54
BsrDI GCAATG_nn'
Cuts at: 2222 2222
Size: 4310
BsrFI r'CCGG_y
Cuts at: 2308 2489 2698 2308
Size: 181 209 3920
Fragments arranged by size:
3920 209 181
BssSI C'ACGA_G
Cuts at: 2581 2939 2581
Size: 358 3952
BstAPI GCAn_nnn'nTGC
Cuts at: 1687 1687
Size: 4310
BstBI TT'CG_AA
Cuts at: 2671 2671
Size: 4310
BstYI r'GATC_y
Cuts at: 1 371 632 1077 1335 1802 1825 2160 2406
Size: 370 261 445 258 467 23 335 246
Cuts at: 2406 3418 3504 3516 3862 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 445 370 346 335 261
258 246 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2260 2441 2604 2260
Size: 181 163 3966
Fragments arranged by size:
3966 181 163
BtsI GCAGTG_nn'
Cuts at: 398 1553 398
Size: 1155 3155
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 459 494 459
Size: 35 4275
DraI TTT'AAA
Cuts at: 1309 1309
Size: 4310
DrdI GACnn_nn'nnGTC
Cuts at: 2016 2874 2016
Size: 858 3452
EaeI y'GGCC_r
Cuts at: 203 1007 1895 2069 2460 2487 3832 203
Size: 804 888 174 391 27 1345 681
Fragments arranged by size:
1345 888 804 681 391 174 27
EagI C'GGCC_G
Cuts at: 203 1895 203
Size: 1692 2618
EarI CTCTTCn'nnn_
Cuts at: 616 2333 2543 616
Size: 1717 210 2383
Fragments arranged by size:
2383 1717 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2518 2828 2974 4120 2518
Size: 310 146 1146 2708
Fragments arranged by size:
2708 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1322 1322
Size: 4310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 388 597 999 2135 2567 3314 3582 259
Size: 129 209 402 1136 432 747 268 987
Fragments arranged by size:
1136 987 747 432 402 268 209 129
EcoNI CCTnn'n_nnAGG
Cuts at: 1153 1153
Size: 4310
EcoO109I rG'GnC_Cy
Cuts at: 182 1449 182
Size: 1267 3043
EcoRI G'AATT_C
Cuts at: 503 1111 1314 503
Size: 608 203 3499
Fragments arranged by size:
3499 608 203
EcoRV GAT'ATC
Cuts at: 795 795
Size: 4310
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2329 2329
Size: 4310
FspI TGC'GCA
Cuts at: 2091 2091
Size: 4310
HaeII r_GCGC'y
Cuts at: 193 1744 1992 3014 3993 193
Size: 1551 248 1022 979 510
Fragments arranged by size:
1551 1022 979 510 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 947 952 979 984 2152 2182 2184 2214 947
Size: 5 27 5 1168 30 2 30 3043
Fragments arranged by size:
3043 1168 30 30 27 5 5 2
HincII GTy'rAC
Cuts at: 456 512 1140 1349 456
Size: 56 628 209 3417
Fragments arranged by size:
3417 628 209 56
KasI G'GCGC_C
Cuts at: 189 1740 1988 189
Size: 1551 248 2511
Fragments arranged by size:
2511 1551 248
KpnI G_GTAC'C
Cuts at: 1330 1330
Size: 4310
MluI A'CGCG_T
Cuts at: 3743 3743
Size: 4310
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 610 2981 3165 610
Size: 2371 184 1755
Fragments arranged by size:
2371 1755 184
MscI TGG'CCA
Cuts at: 1009 2071 1009
Size: 1062 3248
MslI CAynn'nnrTG
Cuts at: 2299 2426 2299
Size: 127 4183
MspA1I CmG'CkG
Cuts at: 155 161 1089 1421 1735 2095 3108 3353 155
Size: 6 928 332 314 360 1013 245 1112
Fragments arranged by size:
1112 1013 928 360 332 314 245 6
NaeI GCC'GGC
Cuts at: 2491 2491
Size: 4310
NarI GG'CG_CC
Cuts at: 190 1741 1989 190
Size: 1551 248 2511
Fragments arranged by size:
2511 1551 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4310
NgoMIV G'CCGG_C
Cuts at: 2489 2489
Size: 4310
NspI r_CATG'y
Cuts at: 219 1244 1363 1863 2770 219
Size: 1025 119 500 907 1759
Fragments arranged by size:
1759 1025 907 500 119
PciI A'CATG_T
Cuts at: 2766 2766
Size: 4310
PfoI T'CCnGG_A
Cuts at: 1259 1259
Size: 4310
PmeI GTTT'AAAC
Cuts at: 1309 1309
Size: 4310
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3449 3481 3449
Size: 32 4278
PpuMI rG'GwC_Cy
Cuts at: 182 182
Size: 4310
PstI C_TGCA'G
Cuts at: 405 1357 2042 405
Size: 952 685 2673
Fragments arranged by size:
2673 952 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4310
PvuII CAG'CTG
Cuts at: 1089 1735 2095 1089
Size: 646 360 3304
Fragments arranged by size:
3304 646 360
RsrII CG'GwC_CG
Cuts at: 2505 2505
Size: 4310
SacI G_AGCT'C
Cuts at: 1324 1324
Size: 4310
SalI G'TCGA_C
Cuts at: 1347 1347
Size: 4310
SanDI GG'GwC_CC
Cuts at: 182 182
Size: 4310
SapI GCTCTTCn'nnn_
Cuts at: 2333 2543 2333
Size: 210 4100
SbfI CC_TGCA'GG
Cuts at: 1357 1357
Size: 4310
ScaI AGT'ACT
Cuts at: 3827 3827
Size: 4310
SfcI C'TryA_G
Cuts at: 33 272 302 401 903 1353 2038 3031 3222
Size: 239 30 99 502 450 685 993 191
Cuts at: 3222 33
Size: 1121
Fragments arranged by size:
1121 993 685 502 450 239 191 99
30
SfoI GGC'GCC
Cuts at: 191 1742 1990 191
Size: 1551 248 2511
Fragments arranged by size:
2511 1551 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4310
SmaI CCC'GGG
Cuts at: 1332 1332
Size: 4310
SmlI C'TyrA_G
Cuts at: 1465 2380 2872 3134 1465
Size: 915 492 262 2641
Fragments arranged by size:
2641 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4310
SphI G_CATG'C
Cuts at: 219 1244 1363 1863 219
Size: 1025 119 500 2666
Fragments arranged by size:
2666 1025 500 119
SspI AAT'ATT
Cuts at: 810 834 810
Size: 24 4286
StyI C'CwwG_G
Cuts at: 107 420 1444 1798 107
Size: 313 1024 354 2619
Fragments arranged by size:
2619 1024 354 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 497 1893 2026 2485 2694 497
Size: 1396 133 459 209 2113
Fragments arranged by size:
2113 1396 459 209 133
TatI w'GTAC_w
Cuts at: 3825 3825
Size: 4310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 854 886 3827 3859 854
Size: 32 2941 32 1305
Fragments arranged by size:
2941 1305 32 32
Tth111I GACn'n_nGTC
Cuts at: 2107 2107
Size: 4310
XbaI T'CTAG_A
Cuts at: 1341 1341
Size: 4310
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 475 813 1805 475
Size: 338 992 2980
Fragments arranged by size:
2980 992 338
XmaI C'CCGG_G
Cuts at: 1330 1330
Size: 4310
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI StuI SwaI XhoI XmnI ZraI