Restriction map for
pF1KB6566
(Circular) (Six-base) MAPSORT of: pF1KB6566.seq Check: 9528 from: 1 to: 4313
pF1KB6566 4313 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1351 1351
Size: 4313
Acc65I G'GTAC_C
Cuts at: 452 1329 452
Size: 877 3436
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 748 769 929 953 1000 2138 2570 3317 3585
Size: 21 160 24 47 1138 432 747 268
Cuts at: 3585 748
Size: 1476
Fragments arranged by size:
1476 1138 747 432 268 160 47 24
21
AflIII A'CryG_T
Cuts at: 1665 2769 3746 1665
Size: 1104 977 2232
Fragments arranged by size:
2232 1104 977
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AhdI GACnn_n'nnGTC
Cuts at: 1215 1215
Size: 4313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1253 1253
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 516 630 1128 1618 3185 516
Size: 114 498 490 1567 1644
Fragments arranged by size:
1644 1567 498 490 114
ApaI G_GGCC'C
Cuts at: 476 476
Size: 4313
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 279 1258 1333 279
Size: 979 75 3259
Fragments arranged by size:
3259 979 75
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 452 535 832 1329 1743 1991 2026 452
Size: 83 297 497 414 248 35 2739
Fragments arranged by size:
2739 497 414 297 248 83 35
BanII G_rGCy'C
Cuts at: 226 476 1107 1327 2357 226
Size: 250 631 220 1030 2182
Fragments arranged by size:
2182 1030 631 250 220
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbsI GAAGACnn'nnnn_
Cuts at: 1076 1076
Size: 4313
BbvCI CC'TCA_GC
Cuts at: 781 781
Size: 4313
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 822 856 1239 1273 822
Size: 34 383 34 3862
Fragments arranged by size:
3862 383 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 252 2211 2972 4313 252
Size: 1959 761 1341 252
Fragments arranged by size:
1959 1341 761 252
BclI T'GATC_A
Cuts at: 1130 1833 1130
Size: 703 3610
BglI GCCn_nnn'nGGC
Cuts at: 164 412 586 1111 164
Size: 248 174 525 3366
Fragments arranged by size:
3366 525 248 174
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1048 1425 1048
Size: 377 3936
Bme1580I G_kGCm'C
Cuts at: 476 835 1938 2031 3087 476
Size: 359 1103 93 1056 1702
Fragments arranged by size:
1702 1103 1056 359 93
BmgBI CAC'GTC
Cuts at: 143 143
Size: 4313
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 537 727 958 1246 430
Size: 107 190 231 288 3497
Fragments arranged by size:
3497 288 231 190 107
Bpu10I CC'TnA_GC
Cuts at: 781 781
Size: 4313
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1489 2368 2860 3158 1045
Size: 444 879 492 298 2200
Fragments arranged by size:
2200 879 492 444 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 715 1827 3864 6
Size: 709 1112 2037 455
Fragments arranged by size:
2037 1112 709 455
BsaHI Gr'CG_yC
Cuts at: 308 1744 1992 2694 4190 308
Size: 1436 248 702 1496 431
Fragments arranged by size:
1496 1436 702 431 248
BsaWI w'CCGG_w
Cuts at: 896 1502 2023 2701 2975 3122 3533 896
Size: 606 521 678 274 147 411 1676
Fragments arranged by size:
1676 678 606 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 208 238 788 818 208
Size: 30 550 30 3703
Fragments arranged by size:
3703 550 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 778 267
Size: 511 3802
BseYI C'CCAG_C
Cuts at: 208 1106 1148 1152 1738 2496 3073 3569 208
Size: 898 42 4 586 758 577 496 952
Fragments arranged by size:
952 898 758 586 577 496 42 4
BsiEI CG_ry'CG
Cuts at: 104 234 375 1855 1901 3109 104
Size: 130 141 1480 46 1208 1308
Fragments arranged by size:
1480 1308 1208 141 130 46
BsiHKAI G_wGCw'C
Cuts at: 1327 2105 2295 3087 1327
Size: 778 190 792 2553
Fragments arranged by size:
2553 792 778 190
BsmI GAATG_Cn'
Cuts at: 212 212
Size: 4313
BsmBI CGTCTCn'nnnn_
Cuts at: 610 610
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 226 476 835 1107 1327 1938 2031 2105 2295
Size: 250 359 272 220 611 93 74 190
Cuts at: 2295 2357 3087 226
Size: 62 730 1452
Fragments arranged by size:
1452 730 611 359 272 250 220 190
93 74 62
BspEI T'CCGG_A
Cuts at: 3533 3533
Size: 4313
BspHI T'CATG_A
Cuts at: 3489 3489
Size: 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 524 974 1010 1363 1577 1879 2260 524
Size: 450 36 353 214 302 381 2577
Fragments arranged by size:
2577 450 381 353 302 214 36
BsrBI CCG'CTC
Cuts at: 50 826 1179 2605 2659 4143 4308 50
Size: 776 353 1426 54 1484 165 55
Fragments arranged by size:
1484 1426 776 353 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2225 2225
Size: 4313
BsrFI r'CCGG_y
Cuts at: 2311 2492 2701 2311
Size: 181 209 3923
Fragments arranged by size:
3923 209 181
BssHII G'CGCG_C
Cuts at: 203 402 1093 203
Size: 199 691 3423
Fragments arranged by size:
3423 691 199
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 2674 2674
Size: 4313
BstYI r'GATC_y
Cuts at: 1 440 848 1338 1805 1828 2163 2409 3421
Size: 439 408 490 467 23 335 246 1012
Cuts at: 3421 3507 3519 3865 1
Size: 86 12 346 449
Fragments arranged by size:
1012 490 467 449 439 408 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 234 234
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1172 2263 2444 2607 1172
Size: 1091 181 163 2878
Fragments arranged by size:
2878 1091 181 163
BtsI GCAGTG_nn'
Cuts at: 1556 1556
Size: 4313
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 137 231 416 692 884 957 1898 2072 2463
Size: 94 185 276 192 73 941 174 391
Cuts at: 2463 2490 3835 137
Size: 27 1345 615
Fragments arranged by size:
1345 941 615 391 276 192 185 174
94 73 27
EagI C'GGCC_G
Cuts at: 231 1898 231
Size: 1667 2646
EarI CTCTTCn'nnn_
Cuts at: 728 2336 2546 728
Size: 1608 210 2495
Fragments arranged by size:
2495 1608 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 243 2521 2831 2977 4123 243
Size: 2278 310 146 1146 433
Fragments arranged by size:
2278 1146 433 310 146
EcoICRI GAG'CTC
Cuts at: 1325 1325
Size: 4313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 537 727 748 769 929 953 958 1000
Size: 107 190 21 21 160 24 5 42
Cuts at: 1000 1246 2138 2570 3317 3585 430
Size: 246 892 432 747 268 1158
Fragments arranged by size:
1158 892 747 432 268 246 190 160
107 42 24 21 21 5
EcoNI CCTnn'n_nnAGG
Cuts at: 394 1258 394
Size: 864 3449
EcoO109I rG'GnC_Cy
Cuts at: 284 466 473 513 1302 1452 284
Size: 182 7 40 789 150 3145
Fragments arranged by size:
3145 789 182 150 40 7
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 634 667 2094 634
Size: 33 1427 2853
Fragments arranged by size:
2853 1427 33
HaeII r_GCGC'y
Cuts at: 1129 1747 1995 3017 3996 1129
Size: 618 248 1022 979 1446
Fragments arranged by size:
1446 1022 979 618 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 788 820 1024 1056 1201 1233 2155 2185 2187
Size: 32 204 32 145 32 922 30 2
Cuts at: 2187 2217 788
Size: 30 2884
Fragments arranged by size:
2884 922 204 145 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1352 1352
Size: 4313
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 456 1333 456
Size: 877 3436
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 347 2984 3168 347
Size: 2637 184 1492
Fragments arranged by size:
2637 1492 184
MscI TGG'CCA
Cuts at: 694 959 2074 694
Size: 265 1115 2933
Fragments arranged by size:
2933 1115 265
MslI CAynn'nnrTG
Cuts at: 640 2302 2429 640
Size: 1662 127 2524
Fragments arranged by size:
2524 1662 127
MspA1I CmG'CkG
Cuts at: 236 1047 1110 1136 1152 1200 1424 1738 2098
Size: 811 63 26 16 48 224 314 360
Cuts at: 2098 3111 3356 236
Size: 1013 245 1193
Fragments arranged by size:
1193 1013 811 360 314 245 224 63
48 26 16
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NotI GC'GGCC_GC
Cuts at: 231 231
Size: 4313
NspI r_CATG'y
Cuts at: 864 1366 1866 2773 864
Size: 502 500 907 2404
Fragments arranged by size:
2404 907 502 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PfoI T'CCnGG_A
Cuts at: 509 553 509
Size: 44 4269
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 513 1302 513
Size: 789 3524
PspOMI G'GGCC_C
Cuts at: 472 472
Size: 4313
PspXI vC'TCGA_Gb
Cuts at: 1258 1258
Size: 4313
PstI C_TGCA'G
Cuts at: 569 594 1040 1360 2045 569
Size: 25 446 320 685 2837
Fragments arranged by size:
2837 685 446 320 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 1047 1110 1152 1738 2098 1047
Size: 63 42 586 360 3262
Fragments arranged by size:
3262 586 360 63 42
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 1327 1327
Size: 4313
SacII CC_GC'GG
Cuts at: 237 237
Size: 4313
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 728 2336 2546 728
Size: 1608 210 2495
Fragments arranged by size:
2495 1608 210
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 117 486 565 590 1036 1356 2041 3034
Size: 84 369 79 25 446 320 685 993
Cuts at: 3034 3225 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 446 369 320 191 84
79 25
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1024 1258 1468 2383 2875 3137 1024
Size: 234 210 915 492 262 2200
Fragments arranged by size:
2200 915 492 262 234 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StuI AGG'CCT
Cuts at: 158 725 158
Size: 567 3746
StyI C'CwwG_G
Cuts at: 1064 1298 1447 1801 1064
Size: 234 149 354 3576
Fragments arranged by size:
3576 354 234 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 715 1896 2029 2488 2697 715
Size: 1181 133 459 209 2331
Fragments arranged by size:
2331 1181 459 209 133
TatI w'GTAC_w
Cuts at: 3828 3828
Size: 4313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 208 240 3830 3862 208
Size: 32 3590 32 659
Fragments arranged by size:
3590 659 32 32
Tth111I GACn'n_nGTC
Cuts at: 318 2110 318
Size: 1792 2521
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 702 1808 702
Size: 1106 3207
XhoI C'TCGA_G
Cuts at: 1258 1258
Size: 4313
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BfrBI BmtI BsgI BsiWI BsrGI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XmnI
ZraI