Restriction map for
pF1KB6567
(Circular) (Six-base) MAPSORT of: pF1KB6567.seq Check: 3736 from: 1 to: 4235
pF1KB6567 4235 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1273 1273
Size: 4235
Acc65I G'GTAC_C
Cuts at: 1251 1251
Size: 4235
AclI AA'CG_TT
Cuts at: 4075 4075
Size: 4235
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 494 503 548 2060 2492 3239 3507 494
Size: 9 45 1512 432 747 268 1222
Fragments arranged by size:
1512 1222 747 432 268 45 9
AflIII A'CryG_T
Cuts at: 397 1587 2691 3668 397
Size: 1190 1104 977 964
Fragments arranged by size:
1190 1104 977 964
AgeI A'CCGG_T
Cuts at: 2623 2623
Size: 4235
AleI CACnn'nnGTG
Cuts at: 1105 1105
Size: 4235
AlwNI CAG_nnn'CTG
Cuts at: 753 1090 1102 1540 3107 753
Size: 337 12 438 1567 1881
Fragments arranged by size:
1881 1567 438 337 12
ApaLI G'TGCA_C
Cuts at: 3005 3005
Size: 4235
ApoI r'AATT_y
Cuts at: 853 870 1116 1239 3588 3844 853
Size: 17 246 123 2349 256 1244
Fragments arranged by size:
2349 1244 256 246 123 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4235
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
AvaI C'yCGr_G
Cuts at: 1255 1255
Size: 4235
BamHI G'GATC_C
Cuts at: 1260 1260
Size: 4235
BanI G'GyrC_C
Cuts at: 1181 1251 1665 1913 1948 1181
Size: 70 414 248 35 3468
Fragments arranged by size:
3468 414 248 70 35
BanII G_rGCy'C
Cuts at: 1249 2279 1249
Size: 1030 3205
BbeI G_GCGC'C
Cuts at: 1669 1917 1669
Size: 248 3987
BbsI GAAGACnn'nnnn_
Cuts at: 225 482 529 225
Size: 257 47 3931
Fragments arranged by size:
3931 257 47
BciVI GTATCCnnnnn_n'
Cuts at: 2133 2894 4235 2133
Size: 761 1341 2133
Fragments arranged by size:
2133 1341 761
BclI T'GATC_A
Cuts at: 1755 1755
Size: 4235
BglII A'GATC_T
Cuts at: 1750 3787 1750
Size: 2037 2198
BlpI GC'TnA_GC
Cuts at: 781 1347 781
Size: 566 3669
Bme1580I G_kGCm'C
Cuts at: 1860 1953 3009 1860
Size: 93 1056 3086
Fragments arranged by size:
3086 1056 93
BmgBI CAC'GTC
Cuts at: 398 398
Size: 4235
BmrI ACTGGGnnnn_n'
Cuts at: 519 1447 1528 1863 4007 519
Size: 928 81 335 2144 747
Fragments arranged by size:
2144 928 747 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 195 2602 195
Size: 2407 1828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 775 946 475
Size: 300 171 3764
Fragments arranged by size:
3764 300 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 165 1411 2290 2782 3080 165
Size: 1246 879 492 298 1320
Fragments arranged by size:
1320 1246 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3939 3939
Size: 4235
BsaAI yAC'GTr
Cuts at: 1588 1588
Size: 4235
BsaBI GATnn'nnATC
Cuts at: 6 1749 3786 6
Size: 1743 2037 455
Fragments arranged by size:
2037 1743 455
BsaHI Gr'CG_yC
Cuts at: 1666 1914 2616 4112 1666
Size: 248 702 1496 1789
Fragments arranged by size:
1789 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1227 1424 1945 2623 2897 3044 3455 1227
Size: 197 521 678 274 147 411 2007
Fragments arranged by size:
2007 678 521 411 274 197 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 448 478 448
Size: 30 4205
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 472 790 943 204
Size: 268 318 153 3496
Fragments arranged by size:
3496 318 268 153
BseYI C'CCAG_C
Cuts at: 556 1660 2418 2995 3491 556
Size: 1104 758 577 496 1300
Fragments arranged by size:
1300 1104 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 510 709 1146 1172 510
Size: 199 437 26 3573
Fragments arranged by size:
3573 437 199 26
BsiEI CG_ry'CG
Cuts at: 104 1777 1823 3031 104
Size: 1673 46 1208 1308
Fragments arranged by size:
1673 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1249 2027 2217 3009 1249
Size: 778 190 792 2475
Fragments arranged by size:
2475 792 778 190
BsmI GAATG_Cn'
Cuts at: 600 659 600
Size: 59 4176
Bsp1286I G_dGCh'C
Cuts at: 1249 1860 1953 2027 2217 2279 3009 1249
Size: 611 93 74 190 62 730 2475
Fragments arranged by size:
2475 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3455 3455
Size: 4235
BspHI T'CATG_A
Cuts at: 3411 3411
Size: 4235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1205 1285 1499 1801 2182 1205
Size: 80 214 302 381 3258
Fragments arranged by size:
3258 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 2527 2581 4065 4230 50
Size: 2477 54 1484 165 55
Fragments arranged by size:
2477 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2147 2147
Size: 4235
BsrFI r'CCGG_y
Cuts at: 2233 2414 2623 2233
Size: 181 209 3845
Fragments arranged by size:
3845 209 181
BssSI C'ACGA_G
Cuts at: 2506 2864 2506
Size: 358 3877
BstAPI GCAn_nnn'nTGC
Cuts at: 1102 1612 1102
Size: 510 3725
BstBI TT'CG_AA
Cuts at: 957 2596 957
Size: 1639 2596
BstXI CCAn_nnnn'nTGG
Cuts at: 1107 1107
Size: 4235
BstYI r'GATC_y
Cuts at: 1 1260 1727 1750 2085 2331 3343 3429 3441
Size: 1259 467 23 335 246 1012 86 12
Cuts at: 3441 3787 1
Size: 346 449
Fragments arranged by size:
1259 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 227 824 107
Size: 120 597 3518
Fragments arranged by size:
3518 597 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2185 2366 2529 2185
Size: 181 163 3891
Fragments arranged by size:
3891 181 163
BtsI GCAGTG_nn'
Cuts at: 254 1100 1478 254
Size: 846 378 3011
Fragments arranged by size:
3011 846 378
ClaI AT'CG_AT
Cuts at: 974 974
Size: 4235
DraI TTT'AAA
Cuts at: 156 420 663 717 1234 156
Size: 264 243 54 517 3157
Fragments arranged by size:
3157 517 264 243 54
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2799 1941
Size: 858 3377
EaeI y'GGCC_r
Cuts at: 224 1820 1994 2385 2412 3757 224
Size: 1596 174 391 27 1345 702
Fragments arranged by size:
1596 1345 702 391 174 27
EagI C'GGCC_G
Cuts at: 1820 1820
Size: 4235
EarI CTCTTCn'nnn_
Cuts at: 164 479 767 1227 2258 2468 164
Size: 315 288 460 1031 210 1931
Fragments arranged by size:
1931 1031 460 315 288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2443 2753 2899 4045 2443
Size: 310 146 1146 2633
Fragments arranged by size:
2633 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1247 1247
Size: 4235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 494 503 548 775 946 2060 2492 3239
Size: 19 9 45 227 171 1114 432 747
Cuts at: 3239 3507 475
Size: 268 1203
Fragments arranged by size:
1203 1114 747 432 268 227 171 45
19 9
EcoO109I rG'GnC_Cy
Cuts at: 750 1374 750
Size: 624 3611
EcoRI G'AATT_C
Cuts at: 1116 1239 1116
Size: 123 4112
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 925 1017 2254 925
Size: 92 1237 2906
Fragments arranged by size:
2906 1237 92
FspI TGC'GCA
Cuts at: 2016 2016
Size: 4235
HaeII r_GCGC'y
Cuts at: 1669 1917 2939 3918 1669
Size: 248 1022 979 1986
Fragments arranged by size:
1986 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 448 480 552 584 2077 2107 2109 2139 448
Size: 32 72 32 1493 30 2 30 2544
Fragments arranged by size:
2544 1493 72 32 32 30 30 2
HincII GTy'rAC
Cuts at: 339 884 1016 1274 339
Size: 545 132 258 3300
Fragments arranged by size:
3300 545 258 132
HpaI GTT'AAC
Cuts at: 339 339
Size: 4235
KasI G'GCGC_C
Cuts at: 1665 1913 1665
Size: 248 3987
KpnI G_GTAC'C
Cuts at: 1255 1255
Size: 4235
MluI A'CGCG_T
Cuts at: 3668 3668
Size: 4235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2906 3090 2906
Size: 184 4051
MscI TGG'CCA
Cuts at: 226 1996 226
Size: 1770 2465
MslI CAynn'nnrTG
Cuts at: 503 1105 2224 2351 503
Size: 602 1119 127 2387
Fragments arranged by size:
2387 1119 602 127
MspA1I CmG'CkG
Cuts at: 1346 1660 2020 3033 3278 1346
Size: 314 360 1013 245 2303
Fragments arranged by size:
2303 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2416 2416
Size: 4235
NarI GG'CG_CC
Cuts at: 1666 1914 1666
Size: 248 3987
NcoI C'CATG_G
Cuts at: 107 227 107
Size: 120 4115
NdeI CA'TA_TG
Cuts at: 846 846
Size: 4235
NgoMIV G'CCGG_C
Cuts at: 2414 2414
Size: 4235
NspI r_CATG'y
Cuts at: 1288 1788 2695 1288
Size: 500 907 2828
Fragments arranged by size:
2828 907 500
PciI A'CATG_T
Cuts at: 2691 2691
Size: 4235
PflMI CCAn_nnn'nTGG
Cuts at: 753 753
Size: 4235
PfoI T'CCnGG_A
Cuts at: 752 752
Size: 4235
PmeI GTTT'AAAC
Cuts at: 1234 1234
Size: 4235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 336 368 3374 3406 336
Size: 32 3006 32 1165
Fragments arranged by size:
3006 1165 32 32
PpuMI rG'GwC_Cy
Cuts at: 750 750
Size: 4235
PstI C_TGCA'G
Cuts at: 261 1282 1967 261
Size: 1021 685 2529
Fragments arranged by size:
2529 1021 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4235
PvuII CAG'CTG
Cuts at: 1660 2020 1660
Size: 360 3875
RsrII CG'GwC_CG
Cuts at: 2430 2430
Size: 4235
SacI G_AGCT'C
Cuts at: 1249 1249
Size: 4235
SalI G'TCGA_C
Cuts at: 1272 1272
Size: 4235
SapI GCTCTTCn'nnn_
Cuts at: 2258 2468 2258
Size: 210 4025
SbfI CC_TGCA'GG
Cuts at: 1282 1282
Size: 4235
ScaI AGT'ACT
Cuts at: 358 3752 358
Size: 3394 841
SfcI C'TryA_G
Cuts at: 33 257 1278 1963 2956 3147 33
Size: 224 1021 685 993 191 1121
Fragments arranged by size:
1121 1021 993 685 224 191
SfoI GGC'GCC
Cuts at: 1667 1915 1667
Size: 248 3987
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4235
SmaI CCC'GGG
Cuts at: 1257 1257
Size: 4235
SmlI C'TyrA_G
Cuts at: 180 1390 2305 2797 3059 180
Size: 1210 915 492 262 1356
Fragments arranged by size:
1356 1210 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4235
SphI G_CATG'C
Cuts at: 1288 1788 1288
Size: 500 3735
SspI AAT'ATT
Cuts at: 161 161
Size: 4235
StyI C'CwwG_G
Cuts at: 107 227 992 1040 1369 1723 107
Size: 120 765 48 329 354 2619
Fragments arranged by size:
2619 765 354 329 120 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 501 760 1818 1951 2410 2619 501
Size: 259 1058 133 459 209 2117
Fragments arranged by size:
2117 1058 459 259 209 133
TatI w'GTAC_w
Cuts at: 356 641 3750 356
Size: 285 3109 841
Fragments arranged by size:
3109 841 285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3752 3784 3752
Size: 32 4203
Tth111I GACn'n_nGTC
Cuts at: 2032 2032
Size: 4235
XbaI T'CTAG_A
Cuts at: 1266 1266
Size: 4235
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 234 921 1730 234
Size: 687 809 2739
Fragments arranged by size:
2739 809 687
XmaI C'CCGG_G
Cuts at: 1255 1255
Size: 4235
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII MfeI
NheI NotI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI