Restriction map for
pF1KB6569
(Circular) (Six-base) MAPSORT of: pF1KB6569.seq Check: 6208 from: 1 to: 4238
pF1KB6569 4238 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 153 319 661 945 1543 3110 153
Size: 166 342 284 598 1567 1281
Fragments arranged by size:
1567 1281 598 342 284 166
ApaI G_GGCC'C
Cuts at: 283 365 536 1122 283
Size: 82 171 586 3399
Fragments arranged by size:
3399 586 171 82
ApaLI G'TGCA_C
Cuts at: 948 3008 948
Size: 2060 2178
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 284 708 1258 284
Size: 424 550 3264
Fragments arranged by size:
3264 550 424
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 550 694 1254 1668 1916 1951 550
Size: 144 560 414 248 35 2837
Fragments arranged by size:
2837 560 414 248 144 35
BanII G_rGCy'C
Cuts at: 283 365 536 571 866 1122 1133 1252 2282
Size: 82 171 35 295 256 11 119 1030
Cuts at: 2282 283
Size: 2239
Fragments arranged by size:
2239 1030 295 256 171 119 82 35
11
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 184 184
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BglI GCCn_nnn'nGGC
Cuts at: 287 593 287
Size: 306 3932
BglII A'GATC_T
Cuts at: 1166 1753 3790 1166
Size: 587 2037 1614
Fragments arranged by size:
2037 1614 587
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 283 365 536 555 952 1122 1863 1956 3012
Size: 82 171 19 397 170 741 93 1056
Cuts at: 3012 283
Size: 1509
Fragments arranged by size:
1509 1056 741 397 171 170 93 82
19
BmrI ACTGGGnnnn_n'
Cuts at: 197 349 422 1125 1450 1531 1866 4010 197
Size: 152 73 703 325 81 335 2144 425
Fragments arranged by size:
2144 703 425 335 325 152 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 686 758 814 413
Size: 273 72 56 3837
Fragments arranged by size:
3837 273 72 56
Bpu10I CC'TnA_GC
Cuts at: 1045 1045
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 4238
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 812 842 812
Size: 30 4208
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 112 112
Size: 4238
BseYI C'CCAG_C
Cuts at: 529 1663 2421 2998 3494 529
Size: 1134 758 577 496 1273
Fragments arranged by size:
1273 1134 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 113 113
Size: 4238
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 866 952 1252 2030 2220 3012 866
Size: 86 300 778 190 792 2092
Fragments arranged by size:
2092 792 778 300 190 86
BsmI GAATG_Cn'
Cuts at: 731 1002 731
Size: 271 3967
Bsp1286I G_dGCh'C
Cuts at: 283 365 536 555 571 866 952 1122 1133
Size: 82 171 19 16 295 86 170 11
Cuts at: 1133 1252 1863 1956 2030 2220 2282 3012 283
Size: 119 611 93 74 190 62 730 1509
Fragments arranged by size:
1509 730 611 295 190 171 170 119
93 86 82 74 62 19 16 11
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 786 1288 1502 1804 2185 786
Size: 502 214 302 381 2839
Fragments arranged by size:
2839 502 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 661 1615 661
Size: 954 3284
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 1166 1263 1730 1753 2088 2334 3346 3432
Size: 1165 97 467 23 335 246 1012 86
Cuts at: 3432 3444 3790 1
Size: 12 346 449
Fragments arranged by size:
1165 1012 467 449 346 335 246 97
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 500 599 500
Size: 99 4139
BtgI C'CryG_G
Cuts at: 671 671
Size: 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 272 2188 2369 2532 272
Size: 1916 181 163 1978
Fragments arranged by size:
1978 1916 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 876 1823 1997 2388 2415 3760 876
Size: 947 174 391 27 1345 1354
Fragments arranged by size:
1354 1345 947 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 121 456 871 2261 2471 121
Size: 335 415 1390 210 1888
Fragments arranged by size:
1888 1390 415 335 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 864 1250 864
Size: 386 3852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 686 758 814 2063 2495 3242 3510 413
Size: 273 72 56 1249 432 747 268 1141
Fragments arranged by size:
1249 1141 747 432 273 268 72 56
EcoO109I rG'GnC_Cy
Cuts at: 279 280 300 433 469 496 595 676 703
Size: 1 20 133 36 27 99 81 27
Cuts at: 703 1119 1377 279
Size: 416 258 3140
Fragments arranged by size:
3140 416 258 133 99 81 36 27
27 20 1
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 1132 1164 2080 2110 2112 2142 364
Size: 32 736 32 916 30 2 30 2460
Fragments arranged by size:
2460 916 736 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 366 1136 2909 3093 366
Size: 770 1773 184 1511
Fragments arranged by size:
1773 1511 770 184
MscI TGG'CCA
Cuts at: 878 1999 878
Size: 1121 3117
MslI CAynn'nnrTG
Cuts at: 2227 2354 2227
Size: 127 4111
MspA1I CmG'CkG
Cuts at: 457 942 991 1014 1349 1663 2023 3036 3281
Size: 485 49 23 335 314 360 1013 245
Cuts at: 3281 457
Size: 1414
Fragments arranged by size:
1414 1013 485 360 335 314 245 49
23
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NcoI C'CATG_G
Cuts at: 671 671
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NruI TCG'CGA
Cuts at: 257 257
Size: 4238
NspI r_CATG'y
Cuts at: 564 1291 1791 2698 564
Size: 727 500 907 2104
Fragments arranged by size:
2104 907 727 500
PasI CC'CwG_GG
Cuts at: 153 474 681 1123 153
Size: 321 207 442 3268
Fragments arranged by size:
3268 442 321 207
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PfoI T'CCnGG_A
Cuts at: 429 735 429
Size: 306 3932
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1060 1092 3377 3409 1060
Size: 32 2285 32 1889
Fragments arranged by size:
2285 1889 32 32
PpuMI rG'GwC_Cy
Cuts at: 300 433 469 496 676 703 300
Size: 133 36 27 180 27 3835
Fragments arranged by size:
3835 180 133 36 27 27
PspOMI G'GGCC_C
Cuts at: 279 361 532 1118 279
Size: 82 171 586 3399
Fragments arranged by size:
3399 586 171 82
PstI C_TGCA'G
Cuts at: 324 1285 1970 324
Size: 961 685 2592
Fragments arranged by size:
2592 961 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 457 942 1014 1663 2023 457
Size: 485 72 649 360 2672
Fragments arranged by size:
2672 649 485 360 72
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 866 1252 866
Size: 386 3852
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SanDI GG'GwC_CC
Cuts at: 300 676 703 300
Size: 376 27 3835
Fragments arranged by size:
3835 376 27
SapI GCTCTTCn'nnn_
Cuts at: 121 871 2261 2471 121
Size: 750 1390 210 1888
Fragments arranged by size:
1888 1390 750 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 320 1281 1966 2959 3150 33
Size: 287 961 685 993 191 1121
Fragments arranged by size:
1121 993 961 685 287 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 593 593
Size: 4238
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 710 1260 710
Size: 550 3688
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 564 1291 1791 564
Size: 727 500 3011
Fragments arranged by size:
3011 727 500
StuI AGG'CCT
Cuts at: 587 805 587
Size: 218 4020
StyI C'CwwG_G
Cuts at: 671 1372 1726 671
Size: 701 354 3183
Fragments arranged by size:
3183 701 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1821 1954 2413 2622 1821
Size: 133 459 209 3437
Fragments arranged by size:
3437 459 209 133
TatI w'GTAC_w
Cuts at: 819 3753 819
Size: 2934 1304
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XmaI C'CCGG_G
Cuts at: 708 1258 708
Size: 550 3688
XmnI GAAnn'nnTTC
Cuts at: 898 898
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NruI NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI