Restriction map for
pF1KB6571
(Circular) (Six-base) MAPSORT of: pF1KB6571.seq Check: 5810 from: 1 to: 4238
pF1KB6571 4238 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 664 1214 1276 664
Size: 550 62 3626
Fragments arranged by size:
3626 550 62
Acc65I G'GTAC_C
Cuts at: 1254 1254
Size: 4238
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 2495 3242 3510 2063
Size: 432 747 268 2791
Fragments arranged by size:
2791 747 432 268
AflIII A'CryG_T
Cuts at: 1590 2694 3671 1590
Size: 1104 977 2157
Fragments arranged by size:
2157 1104 977
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1137 1137
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 481 706 1543 3110 481
Size: 225 837 1567 1609
Fragments arranged by size:
1609 1567 837 225
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 529 1048 1196 1242 3591 3847 529
Size: 519 148 46 2349 256 920
Fragments arranged by size:
2349 920 519 256 148 46
AseI AT'TA_AT
Cuts at: 20 1151 20
Size: 1131 3107
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 1258 1258
Size: 4238
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 1254 1668 1916 1951 1254
Size: 414 248 35 3541
Fragments arranged by size:
3541 414 248 35
BanII G_rGCy'C
Cuts at: 1252 2282 1252
Size: 1030 3208
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbsI GAAGACnn'nnnn_
Cuts at: 739 739
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 2136 2897 4238 2136
Size: 761 1341 2136
Fragments arranged by size:
2136 1341 761
BclI T'GATC_A
Cuts at: 381 1758 381
Size: 1377 2861
BfrBI ATG'CAT
Cuts at: 229 229
Size: 4238
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 1863 1956 3012 1863
Size: 93 1056 3089
Fragments arranged by size:
3089 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1450 1531 1866 4010 1450
Size: 81 335 2144 1678
Fragments arranged by size:
2144 1678 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 1058
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1414 2293 2785 3083 1414
Size: 879 492 298 2569
Fragments arranged by size:
2569 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 582 3942 582
Size: 3360 878
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 283 1752 3789 6
Size: 277 1469 2037 455
Fragments arranged by size:
2037 1469 455 277
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 350 350
Size: 4238
BseYI C'CCAG_C
Cuts at: 1663 2421 2998 3494 1663
Size: 758 577 496 2407
Fragments arranged by size:
2407 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 932 1231 1252 2030 2220 3012 932
Size: 299 21 778 190 792 2158
Fragments arranged by size:
2158 792 778 299 190 21
Bsp1286I G_dGCh'C
Cuts at: 932 1231 1252 1863 1956 2030 2220 2282 3012
Size: 299 21 611 93 74 190 62 730
Cuts at: 3012 932
Size: 2158
Fragments arranged by size:
2158 730 611 299 190 93 74 62
21
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 810 3414 810
Size: 2604 1634
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1288 1502 1804 2185 1288
Size: 214 302 381 3341
Fragments arranged by size:
3341 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 2236 2417 2626 2236
Size: 181 209 3848
Fragments arranged by size:
3848 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 117 1615 117
Size: 1498 2740
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstYI r'GATC_y
Cuts at: 1 171 895 1263 1730 1753 2088 2334 3346
Size: 170 724 368 467 23 335 246 1012
Cuts at: 3346 3432 3444 3790 1
Size: 86 12 346 449
Fragments arranged by size:
1012 724 467 449 368 346 335 246
170 86 23 12
BstZ17I GTA'TAC
Cuts at: 665 665
Size: 4238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 428 1219 2188 2369 2532 428
Size: 791 969 181 163 2134
Fragments arranged by size:
2134 969 791 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 528 1237 528
Size: 709 3529
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 1823 1997 2388 2415 3760 1823
Size: 174 391 27 1345 2301
Fragments arranged by size:
2301 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2446 2756 2902 4048 2446
Size: 310 146 1146 2636
Fragments arranged by size:
2636 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1250 1250
Size: 4238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1058 2063 2495 3242 3510 1058
Size: 1005 432 747 268 1786
Fragments arranged by size:
1786 1005 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1117 1377 1117
Size: 260 3978
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1672 1920 2942 3921 1672
Size: 248 1022 979 1989
Fragments arranged by size:
1989 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 916 948 990 1022 2080 2110 2112 2142 916
Size: 32 42 32 1058 30 2 30 3012
Fragments arranged by size:
3012 1058 42 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1277 1277
Size: 4238
HindIII A'AGCT_T
Cuts at: 791 980 791
Size: 189 4049
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1258 1258
Size: 4238
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 2909 3093 794
Size: 2115 184 1939
Fragments arranged by size:
2115 1939 184
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 466 727 1138 2227 2354 466
Size: 261 411 1089 127 2350
Fragments arranged by size:
2350 1089 411 261 127
MspA1I CmG'CkG
Cuts at: 1220 1349 1663 2023 3036 3281 1220
Size: 129 314 360 1013 245 2177
Fragments arranged by size:
2177 1013 360 314 245 129
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NsiI A_TGCA'T
Cuts at: 231 231
Size: 4238
NspI r_CATG'y
Cuts at: 233 1291 1791 2698 233
Size: 1058 500 907 1773
Fragments arranged by size:
1773 1058 907 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 706 706
Size: 4238
PfoI T'CCnGG_A
Cuts at: 368 368
Size: 4238
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PsiI TTA'TAA
Cuts at: 337 337
Size: 4238
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 1252 1252
Size: 4238
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 2261 2471 2261
Size: 210 4028
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 473 1281 1966 2959 3150 33
Size: 440 808 685 993 191 1121
Fragments arranged by size:
1121 993 808 685 440 191
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 1393 2308 2800 3062 1393
Size: 915 492 262 2569
Fragments arranged by size:
2569 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 1291 1791 1291
Size: 500 3738
StyI C'CwwG_G
Cuts at: 1372 1726 1372
Size: 354 3884
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1214 1821 1954 2413 2622 1214
Size: 607 133 459 209 2830
Fragments arranged by size:
2830 607 459 209 133
TatI w'GTAC_w
Cuts at: 232 1126 3753 232
Size: 894 2627 717
Fragments arranged by size:
2627 894 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 3755
Size: 32 4206
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BstZ17I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NsiI NspI
PciI PflMI PfoI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HpaI MfeI NcoI NdeI NheI NotI
NruI PacI PasI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI