Restriction map for
pF1KB6572
(Circular) (Six-base) MAPSORT of: pF1KB6572.seq Check: 2060 from: 1 to: 4313
pF1KB6572 4313 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 368 1351 368
Size: 983 3330
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 4153 4153
Size: 4313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 668 883 2138 2570 3317 3585 292
Size: 376 215 1255 432 747 268 1020
Fragments arranged by size:
1255 1020 747 432 376 268 215
AflIII A'CryG_T
Cuts at: 1184 1665 2769 3746 1184
Size: 481 1104 977 1751
Fragments arranged by size:
1751 1104 977 481
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 349 499 349
Size: 150 4163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 283 283
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 881 1618 3185 881
Size: 737 1567 2009
Fragments arranged by size:
2009 1567 737
ApaI G_GGCC'C
Cuts at: 285 1049 285
Size: 764 3549
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 396 957 1317 3666 3922 396
Size: 561 360 2349 256 787
Fragments arranged by size:
2349 787 561 360 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 1058 1333 1058
Size: 275 4038
BamHI G'GATC_C
Cuts at: 773 1338 773
Size: 565 3748
BanI G'GyrC_C
Cuts at: 172 1329 1743 1991 2026 172
Size: 1157 414 248 35 2459
Fragments arranged by size:
2459 1157 414 248 35
BanII G_rGCy'C
Cuts at: 285 544 1049 1327 2357 285
Size: 259 505 278 1030 2241
Fragments arranged by size:
2241 1030 505 278 259
BbeI G_GCGC'C
Cuts at: 1747 1995 1747
Size: 248 4065
BbsI GAAGACnn'nnnn_
Cuts at: 231 955 231
Size: 724 3589
BciVI GTATCCnnnnn_n'
Cuts at: 2211 2972 4313 2211
Size: 761 1341 2211
Fragments arranged by size:
2211 1341 761
BclI T'GATC_A
Cuts at: 566 1833 566
Size: 1267 3046
BglI GCCn_nnn'nGGC
Cuts at: 181 181
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 988 1425 988
Size: 437 3876
Bme1580I G_kGCm'C
Cuts at: 285 1049 1938 2031 3087 285
Size: 764 889 93 1056 1511
Fragments arranged by size:
1511 1056 889 764 93
BmrI ACTGGGnnnn_n'
Cuts at: 1525 1606 1941 4085 1525
Size: 81 335 2144 1753
Fragments arranged by size:
2144 1753 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 394 877 394
Size: 483 3830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1276 1489 2368 2860 3158 1276
Size: 213 879 492 298 2431
Fragments arranged by size:
2431 879 492 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1185 1227 1666 1185
Size: 42 439 3832
Fragments arranged by size:
3832 439 42
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 1744 1992 2694 4190 1744
Size: 248 702 1496 1867
Fragments arranged by size:
1867 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1277 1502 2023 2701 2975 3122 3533 1277
Size: 225 521 678 274 147 411 2057
Fragments arranged by size:
2057 678 521 411 274 225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 704 734 822 852 704
Size: 30 88 30 4165
Fragments arranged by size:
4165 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 938 941 958 938
Size: 3 17 4293
Fragments arranged by size:
4293 17 3
BseYI C'CCAG_C
Cuts at: 1041 1218 1738 2496 3073 3569 1041
Size: 177 520 758 577 496 1785
Fragments arranged by size:
1785 758 577 520 496 177
BsiEI CG_ry'CG
Cuts at: 104 575 1855 1901 3109 104
Size: 471 1280 46 1208 1308
Fragments arranged by size:
1308 1280 1208 471 46
BsiHKAI G_wGCw'C
Cuts at: 544 1327 2105 2295 3087 544
Size: 783 778 190 792 1770
Fragments arranged by size:
1770 792 783 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 756 756
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 285 544 1049 1327 1938 2031 2105 2295 2357
Size: 259 505 278 611 93 74 190 62
Cuts at: 2357 3087 285
Size: 730 1511
Fragments arranged by size:
1511 730 611 505 278 259 190 93
74 62
BspEI T'CCGG_A
Cuts at: 1277 3533 1277
Size: 2256 2057
BspHI T'CATG_A
Cuts at: 440 563 3489 440
Size: 123 2926 1264
Fragments arranged by size:
2926 1264 123
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 1363 1577 1879 2260 710
Size: 653 214 302 381 2763
Fragments arranged by size:
2763 653 381 302 214
BsrBI CCG'CTC
Cuts at: 50 279 2605 2659 4143 4308 50
Size: 229 2326 54 1484 165 55
Fragments arranged by size:
2326 1484 229 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 1020 2225 252
Size: 768 1205 2340
Fragments arranged by size:
2340 1205 768
BsrFI r'CCGG_y
Cuts at: 2311 2492 2701 2311
Size: 181 209 3923
Fragments arranged by size:
3923 209 181
BsrGI T'GTAC_A
Cuts at: 448 448
Size: 4313
BssHII G'CGCG_C
Cuts at: 218 218
Size: 4313
BssSI C'ACGA_G
Cuts at: 2584 2942 2584
Size: 358 3955
BstAPI GCAn_nnn'nTGC
Cuts at: 1690 1690
Size: 4313
BstBI TT'CG_AA
Cuts at: 1002 2674 1002
Size: 1672 2641
BstXI CCAn_nnnn'nTGG
Cuts at: 130 130
Size: 4313
BstYI r'GATC_y
Cuts at: 1 773 1295 1338 1805 1828 2163 2409 3421
Size: 772 522 43 467 23 335 246 1012
Cuts at: 3421 3507 3519 3865 1
Size: 86 12 346 449
Fragments arranged by size:
1012 772 522 467 449 346 335 246
86 43 23 12
BstZ17I GTA'TAC
Cuts at: 369 369
Size: 4313
BtgI C'CryG_G
Cuts at: 107 107
Size: 4313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 2444 2607 2263
Size: 181 163 3969
Fragments arranged by size:
3969 181 163
BtsI GCAGTG_nn'
Cuts at: 494 506 890 1167 1556 494
Size: 12 384 277 389 3251
Fragments arranged by size:
3251 389 384 277 12
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 643 791 2019 2877 643
Size: 148 1228 858 2079
Fragments arranged by size:
2079 1228 858 148
EaeI y'GGCC_r
Cuts at: 132 230 341 572 1100 1898 2072 2463 2490
Size: 98 111 231 528 798 174 391 27
Cuts at: 2490 3835 132
Size: 1345 610
Fragments arranged by size:
1345 798 610 528 391 231 174 111
98 27
EagI C'GGCC_G
Cuts at: 572 1898 572
Size: 1326 2987
EarI CTCTTCn'nnn_
Cuts at: 614 687 2336 2546 614
Size: 73 1649 210 2381
Fragments arranged by size:
2381 1649 210 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2521 2831 2977 4123 2521
Size: 310 146 1146 2711
Fragments arranged by size:
2711 1146 310 146
EcoICRI GAG'CTC
Cuts at: 542 1325 542
Size: 783 3530
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 394 668 877 883 2138 2570 3317 3585
Size: 102 274 209 6 1255 432 747 268
Cuts at: 3585 292
Size: 1020
Fragments arranged by size:
1255 1020 747 432 274 268 209 102
6
EcoO109I rG'GnC_Cy
Cuts at: 282 636 1045 1046 1115 1452 282
Size: 354 409 1 69 337 3143
Fragments arranged by size:
3143 409 354 337 69 1
EcoRI G'AATT_C
Cuts at: 957 1317 957
Size: 360 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1008 2332 1008
Size: 1324 2989
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 982 1238 1747 1995 3017 3996 982
Size: 256 509 248 1022 979 1299
Fragments arranged by size:
1299 1022 979 509 256 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 531 563 704 736 765 797 2155 2185 2187
Size: 32 141 32 29 32 1358 30 2
Cuts at: 2187 2217 531
Size: 30 2627
Fragments arranged by size:
2627 1358 141 32 32 32 30 30
29 2
HincII GTy'rAC
Cuts at: 1352 1352
Size: 4313
KasI G'GCGC_C
Cuts at: 1743 1991 1743
Size: 248 4065
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 683 2984 3168 683
Size: 2301 184 1828
Fragments arranged by size:
2301 1828 184
MscI TGG'CCA
Cuts at: 134 232 343 1102 2074 134
Size: 98 111 759 972 2373
Fragments arranged by size:
2373 972 759 111 98
MslI CAynn'nnrTG
Cuts at: 265 349 499 2302 2429 265
Size: 84 150 1803 127 2149
Fragments arranged by size:
2149 1803 150 127 84
MspA1I CmG'CkG
Cuts at: 126 1424 1738 2098 3111 3356 126
Size: 1298 314 360 1013 245 1083
Fragments arranged by size:
1298 1083 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 1744 1992 1744
Size: 248 4065
NcoI C'CATG_G
Cuts at: 107 107
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NspI r_CATG'y
Cuts at: 196 1181 1366 1866 2773 196
Size: 985 185 500 907 1736
Fragments arranged by size:
1736 985 907 500 185
PasI CC'CwG_GG
Cuts at: 359 359
Size: 4313
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PflMI CCAn_nnn'nTGG
Cuts at: 1098 1214 1098
Size: 116 4197
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PmlI CAC'GTG
Cuts at: 1185 1185
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 636 636
Size: 4313
PspOMI G'GGCC_C
Cuts at: 281 1045 281
Size: 764 3549
PstI C_TGCA'G
Cuts at: 290 501 1360 2045 290
Size: 211 859 685 2558
Fragments arranged by size:
2558 859 685 211
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 126 1738 2098 126
Size: 1612 360 2341
Fragments arranged by size:
2341 1612 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 544 1327 544
Size: 783 3530
SalI G'TCGA_C
Cuts at: 1350 1350
Size: 4313
SanDI GG'GwC_CC
Cuts at: 636 636
Size: 4313
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 286 497 873 1356 2041 3034 3225 33
Size: 253 211 376 483 685 993 191 1121
Fragments arranged by size:
1121 993 685 483 376 253 211 191
SfoI GGC'GCC
Cuts at: 1745 1993 1745
Size: 248 4065
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SmaI CCC'GGG
Cuts at: 1335 1335
Size: 4313
SmlI C'TyrA_G
Cuts at: 1291 1468 2383 2875 3137 1291
Size: 177 915 492 262 2467
Fragments arranged by size:
2467 915 492 262 177
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StuI AGG'CCT
Cuts at: 813 813
Size: 4313
StyI C'CwwG_G
Cuts at: 107 347 896 1447 1801 107
Size: 240 549 551 354 2619
Fragments arranged by size:
2619 551 549 354 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 351 889 1896 2029 2488 2697 351
Size: 538 1007 133 459 209 1967
Fragments arranged by size:
1967 1007 538 459 209 133
TatI w'GTAC_w
Cuts at: 448 3828 448
Size: 3380 933
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 741 1215 1808 741
Size: 474 593 3246
Fragments arranged by size:
3246 593 474
XmaI C'CCGG_G
Cuts at: 1333 1333
Size: 4313
XmnI GAAnn'nnTTC
Cuts at: 1285 1285
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BstEII Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PfoI PshAI PsiI PspXI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI