Restriction map for
pF1KB6579
(Circular) (Six-base) MAPSORT of: pF1KB6579.seq Check: 6709 from: 1 to: 4244
pF1KB6579 4244 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 4084 4084
Size: 4244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 2069 2501 3248 3516 334
Size: 1735 432 747 268 1062
Fragments arranged by size:
1735 1062 747 432 268
AfeI AGC'GCT
Cuts at: 874 874
Size: 4244
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 1549 3116 1549
Size: 1567 2677
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 406 1264 406
Size: 858 3386
AvrII C'CTAG_G
Cuts at: 193 193
Size: 4244
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 371 1018 1074 1260 1674 1922 1957 371
Size: 647 56 186 414 248 35 2658
Fragments arranged by size:
2658 647 414 248 186 56 35
BanII G_rGCy'C
Cuts at: 1258 2288 1258
Size: 1030 3214
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BbsI GAAGACnn'nnnn_
Cuts at: 125 125
Size: 4244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1218 1252 1218
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 313 2142 2903 4244 313
Size: 1829 761 1341 313
Fragments arranged by size:
1829 1341 761 313
BclI T'GATC_A
Cuts at: 1764 1764
Size: 4244
BglI GCCn_nnn'nGGC
Cuts at: 196 523 846 196
Size: 327 323 3594
Fragments arranged by size:
3594 327 323
BglII A'GATC_T
Cuts at: 365 1759 3796 365
Size: 1394 2037 813
Fragments arranged by size:
2037 1394 813
BlpI GC'TnA_GC
Cuts at: 725 1356 725
Size: 631 3613
Bme1580I G_kGCm'C
Cuts at: 503 605 1021 1869 1962 3018 503
Size: 102 416 848 93 1056 1729
Fragments arranged by size:
1729 1056 848 416 102 93
BmrI ACTGGGnnnn_n'
Cuts at: 357 1456 1537 1872 4016 357
Size: 1099 81 335 2144 585
Fragments arranged by size:
2144 1099 585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1420 2299 2791 3089 1420
Size: 879 492 298 2575
Fragments arranged by size:
2575 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 118 481 934 3948 118
Size: 363 453 3014 414
Fragments arranged by size:
3014 453 414 363
BsaAI yAC'GTr
Cuts at: 273 513 768 1597 273
Size: 240 255 829 2920
Fragments arranged by size:
2920 829 255 240
BsaBI GATnn'nnATC
Cuts at: 6 685 1096 1180 1758 3795 6
Size: 679 411 84 578 2037 455
Fragments arranged by size:
2037 679 578 455 411 84
BsaHI Gr'CG_yC
Cuts at: 188 239 587 1675 1923 2625 4121 188
Size: 51 348 1088 248 702 1496 311
Fragments arranged by size:
1496 1088 702 348 311 248 51
BsaWI w'CCGG_w
Cuts at: 1433 1954 2632 2906 3053 3464 1433
Size: 521 678 274 147 411 2213
Fragments arranged by size:
2213 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 136 166 745 775 136
Size: 30 579 30 3605
Fragments arranged by size:
3605 579 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 424 798 801 804 424
Size: 374 3 3 3864
Fragments arranged by size:
3864 374 3 3
BseYI C'CCAG_C
Cuts at: 1078 1669 2427 3004 3500 1078
Size: 591 758 577 496 1822
Fragments arranged by size:
1822 758 591 577 496
BsiEI CG_ry'CG
Cuts at: 104 803 1786 1832 3040 104
Size: 699 983 46 1208 1308
Fragments arranged by size:
1308 1208 983 699 46
BsiHKAI G_wGCw'C
Cuts at: 332 416 1258 2036 2226 3018 332
Size: 84 842 778 190 792 1558
Fragments arranged by size:
1558 842 792 778 190 84
BsmI GAATG_Cn'
Cuts at: 932 932
Size: 4244
BsmBI CGTCTCn'nnnn_
Cuts at: 883 883
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 332 416 503 605 1021 1258 1869 1962 2036
Size: 84 87 102 416 237 611 93 74
Cuts at: 2036 2226 2288 3018 332
Size: 190 62 730 1558
Fragments arranged by size:
1558 730 611 416 237 190 102 93
87 84 74 62
BspEI T'CCGG_A
Cuts at: 3464 3464
Size: 4244
BspHI T'CATG_A
Cuts at: 959 3420 959
Size: 2461 1783
BspMI ACCTGCnnnn'nnnn_
Cuts at: 840 1294 1508 1810 2191 840
Size: 454 214 302 381 2893
Fragments arranged by size:
2893 454 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2156 2156
Size: 4244
BsrFI r'CCGG_y
Cuts at: 169 559 1207 2242 2423 2632 169
Size: 390 648 1035 181 209 1781
Fragments arranged by size:
1781 1035 648 390 209 181
BssSI C'ACGA_G
Cuts at: 136 937 2515 2873 136
Size: 801 1578 358 1507
Fragments arranged by size:
1578 1507 801 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 513 513
Size: 4244
BstYI r'GATC_y
Cuts at: 1 365 686 1269 1736 1759 2094 2340 3352
Size: 364 321 583 467 23 335 246 1012
Cuts at: 3352 3438 3450 3796 1
Size: 86 12 346 449
Fragments arranged by size:
1012 583 467 449 364 346 335 321
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1086 2194 2375 2538 1086
Size: 1108 181 163 2792
Fragments arranged by size:
2792 1108 181 163
BtsI GCAGTG_nn'
Cuts at: 1487 1487
Size: 4244
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DraIII CAC_nnn'GTG
Cuts at: 600 600
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 850 1950 2808 850
Size: 1100 858 2286
Fragments arranged by size:
2286 1100 858
EaeI y'GGCC_r
Cuts at: 515 800 1829 2003 2394 2421 3766 515
Size: 285 1029 174 391 27 1345 993
Fragments arranged by size:
1345 1029 993 391 285 174 27
EagI C'GGCC_G
Cuts at: 800 1829 800
Size: 1029 3215
EarI CTCTTCn'nnn_
Cuts at: 901 2267 2477 901
Size: 1366 210 2668
Fragments arranged by size:
2668 1366 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 2762 2908 4054 2452
Size: 310 146 1146 2642
Fragments arranged by size:
2642 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 2069 2501 3248 3516 334
Size: 1735 432 747 268 1062
Fragments arranged by size:
1735 1062 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 549 549
Size: 4244
EcoO109I rG'GnC_Cy
Cuts at: 435 987 1383 435
Size: 552 396 3296
Fragments arranged by size:
3296 552 396
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2263 2263
Size: 4244
FseI GG_CCGG'CC
Cuts at: 1211 1211
Size: 4244
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 876 1678 1926 2948 3927 876
Size: 802 248 1022 979 1193
Fragments arranged by size:
1193 1022 979 802 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 2086 2116 2118 2148 136
Size: 32 1918 30 2 30 2232
Fragments arranged by size:
2232 1918 32 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 517 2005 517
Size: 1488 2756
MslI CAynn'nnrTG
Cuts at: 511 916 1024 2233 2360 511
Size: 405 108 1209 127 2395
Fragments arranged by size:
2395 1209 405 127 108
MspA1I CmG'CkG
Cuts at: 724 881 1355 1669 2029 3042 3287 724
Size: 157 474 314 360 1013 245 1681
Fragments arranged by size:
1681 1013 474 360 314 245 157
NaeI GCC'GGC
Cuts at: 171 1209 2425 171
Size: 1038 1216 1990
Fragments arranged by size:
1990 1216 1038
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NgoMIV G'CCGG_C
Cuts at: 169 1207 2423 169
Size: 1038 1216 1990
Fragments arranged by size:
1990 1216 1038
NspI r_CATG'y
Cuts at: 1053 1297 1797 2704 1053
Size: 244 500 907 2593
Fragments arranged by size:
2593 907 500 244
PasI CC'CwG_GG
Cuts at: 1033 1033
Size: 4244
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3383 3415 3383
Size: 32 4212
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 4244
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 724 1669 2029 724
Size: 945 360 2939
Fragments arranged by size:
2939 945 360
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 901 2267 2477 901
Size: 1366 210 2668
Fragments arranged by size:
2668 1366 210
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 1287 1972 2965 3156 33
Size: 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 523 523
Size: 4244
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 1399 2314 2806 3068 1399
Size: 915 492 262 2575
Fragments arranged by size:
2575 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1053 1297 1797 1053
Size: 244 500 3500
Fragments arranged by size:
3500 500 244
StyI C'CwwG_G
Cuts at: 193 449 1378 1732 193
Size: 256 929 354 2705
Fragments arranged by size:
2705 929 354 256
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1092 1827 1960 2419 2628 607
Size: 485 735 133 459 209 2223
Fragments arranged by size:
2223 735 485 459 209 133
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 3761 3793 745
Size: 32 2984 32 1196
Fragments arranged by size:
2984 1196 32 32
Tth111I GACn'n_nGTC
Cuts at: 586 2041 586
Size: 1455 2789
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 1739 642
Size: 1097 3147
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BmgBI BmtI BpmI Bpu10I
BsgI BsiWI BsrGI BssHII BstEII BstZ17I Bsu36I BtgI
ClaI CspCI EcoRV FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI