Restriction map for
pF1KB6580
(Circular) (Six-base) MAPSORT of: pF1KB6580.seq Check: 3809 from: 1 to: 4322
pF1KB6580 4322 bp
With 182 enzymes: SgfI *
April 24, 2009 13:06 ..
AccI GT'mk_AC
Cuts at: 242 1360 242
Size: 1118 3204
Acc65I G'GTAC_C
Cuts at: 1338 1338
Size: 4322
AclI AA'CG_TT
Cuts at: 4162 4162
Size: 4322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2579 3326 3594 2147
Size: 432 747 268 2875
Fragments arranged by size:
2875 747 432 268
AflIII A'CryG_T
Cuts at: 1674 2778 3755 1674
Size: 1104 977 2241
Fragments arranged by size:
2241 1104 977
AgeI A'CCGG_T
Cuts at: 2710 2710
Size: 4322
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 726 758 802 834 726
Size: 32 44 32 4214
Fragments arranged by size:
4214 44 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1627 3194 1627
Size: 1567 2755
ApaLI G'TGCA_C
Cuts at: 3092 3092
Size: 4322
ApoI r'AATT_y
Cuts at: 346 538 1125 1326 3675 3931 346
Size: 192 587 201 2349 256 737
Fragments arranged by size:
2349 737 587 256 201 192
AseI AT'TA_AT
Cuts at: 20 20
Size: 4322
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
AvaI C'yCGr_G
Cuts at: 1342 1342
Size: 4322
BamHI G'GATC_C
Cuts at: 1347 1347
Size: 4322
BanI G'GyrC_C
Cuts at: 1338 1752 2000 2035 1338
Size: 414 248 35 3625
Fragments arranged by size:
3625 414 248 35
BanII G_rGCy'C
Cuts at: 1336 2366 1336
Size: 1030 3292
BbeI G_GCGC'C
Cuts at: 1756 2004 1756
Size: 248 4074
BbsI GAAGACnn'nnnn_
Cuts at: 386 818 386
Size: 432 3890
BbvCI CC'TCA_GC
Cuts at: 839 839
Size: 4322
BciVI GTATCCnnnnn_n'
Cuts at: 2220 2981 4322 2220
Size: 761 1341 2220
Fragments arranged by size:
2220 1341 761
BclI T'GATC_A
Cuts at: 996 1230 1842 996
Size: 234 612 3476
Fragments arranged by size:
3476 612 234
BglII A'GATC_T
Cuts at: 426 1837 3874 426
Size: 1411 2037 874
Fragments arranged by size:
2037 1411 874
BlpI GC'TnA_GC
Cuts at: 1434 1434
Size: 4322
Bme1580I G_kGCm'C
Cuts at: 1947 2040 3096 1947
Size: 93 1056 3173
Fragments arranged by size:
3173 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 608 1534 1615 1950 4094 608
Size: 926 81 335 2144 836
Fragments arranged by size:
2144 926 836 335 81
BmtI G_CTAG'C
Cuts at: 1187 1187
Size: 4322
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 981 2689 981
Size: 1708 2614
Bpu10I CC'TnA_GC
Cuts at: 839 839
Size: 4322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1498 2377 2869 3167 1498
Size: 879 492 298 2653
Fragments arranged by size:
2653 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4026 4026
Size: 4322
BsaAI yAC'GTr
Cuts at: 1675 1675
Size: 4322
BsaBI GATnn'nnATC
Cuts at: 6 455 1289 1836 3873 6
Size: 449 834 547 2037 455
Fragments arranged by size:
2037 834 547 455 449
BsaHI Gr'CG_yC
Cuts at: 1753 2001 2703 4199 1753
Size: 248 702 1496 1876
Fragments arranged by size:
1876 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1511 2032 2710 2984 3131 3542 1511
Size: 521 678 274 147 411 2291
Fragments arranged by size:
2291 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1177 1177
Size: 4322
BseYI C'CCAG_C
Cuts at: 1747 2505 3082 3578 1747
Size: 758 577 496 2491
Fragments arranged by size:
2491 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1052 1052
Size: 4322
BsiEI CG_ry'CG
Cuts at: 104 1864 1910 3118 104
Size: 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1336 2114 2304 3096 1336
Size: 778 190 792 2562
Fragments arranged by size:
2562 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1336 1947 2040 2114 2304 2366 3096 1336
Size: 611 93 74 190 62 730 2562
Fragments arranged by size:
2562 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3542 3542
Size: 4322
BspHI T'CATG_A
Cuts at: 3498 3498
Size: 4322
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1372 1586 1888 2269 1372
Size: 214 302 381 3425
Fragments arranged by size:
3425 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2614 2668 4152 4317 50
Size: 2564 54 1484 165 55
Fragments arranged by size:
2564 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2234 2234
Size: 4322
BsrFI r'CCGG_y
Cuts at: 2320 2501 2710 2320
Size: 181 209 3932
Fragments arranged by size:
3932 209 181
BssSI C'ACGA_G
Cuts at: 2593 2951 2593
Size: 358 3964
BstAPI GCAn_nnn'nTGC
Cuts at: 1699 1699
Size: 4322
BstBI TT'CG_AA
Cuts at: 2683 2683
Size: 4322
BstYI r'GATC_y
Cuts at: 1 426 1347 1814 1837 2172 2418 3430 3516
Size: 425 921 467 23 335 246 1012 86
Cuts at: 3516 3528 3874 1
Size: 12 346 449
Fragments arranged by size:
1012 921 467 449 425 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 243 243
Size: 4322
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2272 2453 2616 2272
Size: 181 163 3978
Fragments arranged by size:
3978 181 163
BtsI GCAGTG_nn'
Cuts at: 1565 1565
Size: 4322
ClaI AT'CG_AT
Cuts at: 1007 1007
Size: 4322
DraI TTT'AAA
Cuts at: 545 1321 545
Size: 776 3546
DrdI GACnn_nn'nnGTC
Cuts at: 2028 2886 2028
Size: 858 3464
EaeI y'GGCC_r
Cuts at: 1907 2081 2472 2499 3844 1907
Size: 174 391 27 1345 2385
Fragments arranged by size:
2385 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1907 1907
Size: 4322
EarI CTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 746 2530 2840 2986 4132 746
Size: 1784 310 146 1146 936
Fragments arranged by size:
1784 1146 936 310 146
EcoICRI GAG'CTC
Cuts at: 1334 1334
Size: 4322
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 2147 2579 3326 3594 2147
Size: 432 747 268 2875
Fragments arranged by size:
2875 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 558 796 1461 558
Size: 238 665 3419
Fragments arranged by size:
3419 665 238
EcoRI G'AATT_C
Cuts at: 1326 1326
Size: 4322
EcoRV GAT'ATC
Cuts at: 154 864 154
Size: 710 3612
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1161 2341 1161
Size: 1180 3142
FspI TGC'GCA
Cuts at: 2103 2103
Size: 4322
HaeII r_GCGC'y
Cuts at: 627 1756 2004 3026 4005 627
Size: 1129 248 1022 979 944
Fragments arranged by size:
1129 1022 979 944 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 297 329 1153 1185 2164 2194 2196 2226 297
Size: 32 824 32 979 30 2 30 2393
Fragments arranged by size:
2393 979 824 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1361 1361
Size: 4322
KasI G'GCGC_C
Cuts at: 1752 2000 1752
Size: 248 4074
KpnI G_GTAC'C
Cuts at: 1342 1342
Size: 4322
MluI A'CGCG_T
Cuts at: 3755 3755
Size: 4322
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2993 3177 2993
Size: 184 4138
MscI TGG'CCA
Cuts at: 2083 2083
Size: 4322
MslI CAynn'nnrTG
Cuts at: 2311 2438 2311
Size: 127 4195
MspA1I CmG'CkG
Cuts at: 1433 1747 2107 3120 3365 1433
Size: 314 360 1013 245 2390
Fragments arranged by size:
2390 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2503 2503
Size: 4322
NarI GG'CG_CC
Cuts at: 1753 2001 1753
Size: 248 4074
NgoMIV G'CCGG_C
Cuts at: 2501 2501
Size: 4322
NheI G'CTAG_C
Cuts at: 1183 1183
Size: 4322
NspI r_CATG'y
Cuts at: 1375 1875 2782 1375
Size: 500 907 2915
Fragments arranged by size:
2915 907 500
PciI A'CATG_T
Cuts at: 2778 2778
Size: 4322
PmeI GTTT'AAAC
Cuts at: 1321 1321
Size: 4322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 3461
Size: 32 4290
PpuMI rG'GwC_Cy
Cuts at: 796 796
Size: 4322
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 817 849 817
Size: 32 4290
PstI C_TGCA'G
Cuts at: 225 1369 2054 225
Size: 1144 685 2493
Fragments arranged by size:
2493 1144 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4322
PvuII CAG'CTG
Cuts at: 1747 2107 1747
Size: 360 3962
RsrII CG'GwC_CG
Cuts at: 2517 2517
Size: 4322
SacI G_AGCT'C
Cuts at: 1336 1336
Size: 4322
SalI G'TCGA_C
Cuts at: 1359 1359
Size: 4322
SapI GCTCTTCn'nnn_
Cuts at: 2345 2555 2345
Size: 210 4112
SbfI CC_TGCA'GG
Cuts at: 1369 1369
Size: 4322
ScaI AGT'ACT
Cuts at: 1058 3839 1058
Size: 2781 1541
SfcI C'TryA_G
Cuts at: 33 221 1365 2050 3043 3234 33
Size: 188 1144 685 993 191 1121
Fragments arranged by size:
1144 1121 993 685 191 188
SfoI GGC'GCC
Cuts at: 1754 2002 1754
Size: 248 4074
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4322
SmaI CCC'GGG
Cuts at: 1344 1344
Size: 4322
SmlI C'TyrA_G
Cuts at: 1477 2392 2884 3146 1477
Size: 915 492 262 2653
Fragments arranged by size:
2653 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4322
SphI G_CATG'C
Cuts at: 1375 1875 1375
Size: 500 3822
SspI AAT'ATT
Cuts at: 255 255
Size: 4322
StyI C'CwwG_G
Cuts at: 1456 1810 1456
Size: 354 3968
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1905 2038 2497 2706 1905
Size: 133 459 209 3521
Fragments arranged by size:
3521 459 209 133
TatI w'GTAC_w
Cuts at: 824 1056 1142 3837 824
Size: 232 86 2695 1309
Fragments arranged by size:
2695 1309 232 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3839 3871 3839
Size: 32 4290
Tth111I GACn'n_nGTC
Cuts at: 2119 2119
Size: 4322
XbaI T'CTAG_A
Cuts at: 1353 1353
Size: 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1817 1817
Size: 4322
XmaI C'CCGG_G
Cuts at: 1342 1342
Size: 4322
XmnI GAAnn'nnTTC
Cuts at: 713 713
Size: 4322
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PciI PmeI PpiI PpuMI PsrI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BpmI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I BtgI CspCI DraIII EcoNI FseI FspAI HindIII
HpaI MfeI NcoI NdeI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI