Restriction map for
pF1KB6585
(Circular) (Six-base) MAPSORT of: pF1KB6585.seq Check: 5273 from: 1 to: 4244
pF1KB6585 4244 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 225 225
Size: 4244
AccI GT'mk_AC
Cuts at: 1282 1282
Size: 4244
Acc65I G'GTAC_C
Cuts at: 1260 1260
Size: 4244
AclI AA'CG_TT
Cuts at: 873 4084 873
Size: 3211 1033
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 428 2069 2501 3248 3516 334
Size: 94 1641 432 747 268 1062
Fragments arranged by size:
1641 1062 747 432 268 94
AflIII A'CryG_T
Cuts at: 1596 2700 3677 1596
Size: 1104 977 2163
Fragments arranged by size:
2163 1104 977
AgeI A'CCGG_T
Cuts at: 2632 2632
Size: 4244
AlwNI CAG_nnn'CTG
Cuts at: 1549 3116 1549
Size: 1567 2677
ApaLI G'TGCA_C
Cuts at: 3014 3014
Size: 4244
ApoI r'AATT_y
Cuts at: 1248 3597 3853 1248
Size: 2349 256 1639
Fragments arranged by size:
2349 1639 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4244
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
AvaI C'yCGr_G
Cuts at: 1264 1264
Size: 4244
BamHI G'GATC_C
Cuts at: 1269 1269
Size: 4244
BanI G'GyrC_C
Cuts at: 371 435 1018 1260 1674 1922 1957 371
Size: 64 583 242 414 248 35 2658
Fragments arranged by size:
2658 583 414 248 242 64 35
BanII G_rGCy'C
Cuts at: 161 1258 2288 161
Size: 1097 1030 2117
Fragments arranged by size:
2117 1097 1030
BbeI G_GCGC'C
Cuts at: 1678 1926 1678
Size: 248 3996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1218 1252 1218
Size: 34 4210
BciVI GTATCCnnnnn_n'
Cuts at: 2142 2903 4244 2142
Size: 761 1341 2142
Fragments arranged by size:
2142 1341 761
BclI T'GATC_A
Cuts at: 854 1764 854
Size: 910 3334
BglI GCCn_nnn'nGGC
Cuts at: 846 846
Size: 4244
BglII A'GATC_T
Cuts at: 365 663 1759 3796 365
Size: 298 1096 2037 813
Fragments arranged by size:
2037 1096 813 298
BlpI GC'TnA_GC
Cuts at: 725 1356 725
Size: 631 3613
Bme1580I G_kGCm'C
Cuts at: 1021 1869 1962 3018 1021
Size: 848 93 1056 2247
Fragments arranged by size:
2247 1056 848 93
BmrI ACTGGGnnnn_n'
Cuts at: 357 1456 1537 1872 4016 357
Size: 1099 81 335 2144 585
Fragments arranged by size:
2144 1099 585 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 599 599
Size: 4244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 841 1420 2299 2791 3089 841
Size: 579 879 492 298 1996
Fragments arranged by size:
1996 879 579 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 883 3948 883
Size: 3065 1179
BsaAI yAC'GTr
Cuts at: 273 1597 273
Size: 1324 2920
BsaBI GATnn'nnATC
Cuts at: 6 859 1096 1180 1758 3795 6
Size: 853 237 84 578 2037 455
Fragments arranged by size:
2037 853 578 455 237 84
BsaHI Gr'CG_yC
Cuts at: 222 1675 1923 2625 4121 222
Size: 1453 248 702 1496 345
Fragments arranged by size:
1496 1453 702 345 248
BsaWI w'CCGG_w
Cuts at: 1110 1433 1954 2632 2906 3053 3464 1110
Size: 323 521 678 274 147 411 1890
Fragments arranged by size:
1890 678 521 411 323 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 572 602 745 775 572
Size: 30 143 30 4041
Fragments arranged by size:
4041 143 30 30
BseYI C'CCAG_C
Cuts at: 178 501 1078 1669 2427 3004 3500 178
Size: 323 577 591 758 577 496 922
Fragments arranged by size:
922 758 591 577 577 496 323
BsiEI CG_ry'CG
Cuts at: 104 1047 1786 1832 3040 104
Size: 943 739 46 1208 1308
Fragments arranged by size:
1308 1208 943 739 46
BsiHKAI G_wGCw'C
Cuts at: 1258 2036 2226 3018 1258
Size: 778 190 792 2484
Fragments arranged by size:
2484 792 778 190
BsmI GAATG_Cn'
Cuts at: 1055 1055
Size: 4244
Bsp1286I G_dGCh'C
Cuts at: 161 1021 1258 1869 1962 2036 2226 2288 3018
Size: 860 237 611 93 74 190 62 730
Cuts at: 3018 161
Size: 1387
Fragments arranged by size:
1387 860 730 611 237 190 93 74
62
BspEI T'CCGG_A
Cuts at: 1110 3464 1110
Size: 2354 1890
BspHI T'CATG_A
Cuts at: 680 959 3420 680
Size: 279 2461 1504
Fragments arranged by size:
2461 1504 279
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1294 1508 1810 2191 1294
Size: 214 302 381 3347
Fragments arranged by size:
3347 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2536 2590 4074 4239 50
Size: 2486 54 1484 165 55
Fragments arranged by size:
2486 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1037 1100 2156 1037
Size: 63 1056 3125
Fragments arranged by size:
3125 1056 63
BsrFI r'CCGG_y
Cuts at: 169 2242 2423 2632 169
Size: 2073 181 209 1781
Fragments arranged by size:
2073 1781 209 181
BssSI C'ACGA_G
Cuts at: 2515 2873 2515
Size: 358 3886
BstAPI GCAn_nnn'nTGC
Cuts at: 1621 1621
Size: 4244
BstBI TT'CG_AA
Cuts at: 2605 2605
Size: 4244
BstXI CCAn_nnnn'nTGG
Cuts at: 513 513
Size: 4244
BstYI r'GATC_y
Cuts at: 1 365 648 663 686 1269 1736 1759 2094
Size: 364 283 15 23 583 467 23 335
Cuts at: 2094 2340 3352 3438 3450 3796 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 583 467 449 364 346 335 283
246 86 23 23 15 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2194 2375 2538 2194
Size: 181 163 3900
Fragments arranged by size:
3900 181 163
BtsI GCAGTG_nn'
Cuts at: 128 797 1487 128
Size: 669 690 2885
Fragments arranged by size:
2885 690 669
DraI TTT'AAA
Cuts at: 1243 1243
Size: 4244
DrdI GACnn_nn'nnGTC
Cuts at: 1950 2808 1950
Size: 858 3386
EaeI y'GGCC_r
Cuts at: 657 794 1829 2003 2394 2421 3766 657
Size: 137 1035 174 391 27 1345 1135
Fragments arranged by size:
1345 1135 1035 391 174 137 27
EagI C'GGCC_G
Cuts at: 1829 1829
Size: 4244
EarI CTCTTCn'nnn_
Cuts at: 113 404 820 2267 2477 113
Size: 291 416 1447 210 1880
Fragments arranged by size:
1880 1447 416 291 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2452 2762 2908 4054 2452
Size: 310 146 1146 2642
Fragments arranged by size:
2642 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1256 1256
Size: 4244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 334 428 599 2069 2501 3248 3516 334
Size: 94 171 1470 432 747 268 1062
Fragments arranged by size:
1470 1062 747 432 268 171 94
EcoO109I rG'GnC_Cy
Cuts at: 987 1383 987
Size: 396 3848
EcoRI G'AATT_C
Cuts at: 1248 1248
Size: 4244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 189 2263 189
Size: 2074 2170
FspI TGC'GCA
Cuts at: 2025 2025
Size: 4244
HaeII r_GCGC'y
Cuts at: 1678 1926 2948 3927 1678
Size: 248 1022 979 1995
Fragments arranged by size:
1995 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 136 168 572 604 2086 2116 2118 2148 136
Size: 32 404 32 1482 30 2 30 2232
Fragments arranged by size:
2232 1482 404 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1283 1283
Size: 4244
KasI G'GCGC_C
Cuts at: 1674 1922 1674
Size: 248 3996
KpnI G_GTAC'C
Cuts at: 1264 1264
Size: 4244
MluI A'CGCG_T
Cuts at: 3677 3677
Size: 4244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2915 3099 2915
Size: 184 4060
MscI TGG'CCA
Cuts at: 796 2005 796
Size: 1209 3035
MslI CAynn'nnrTG
Cuts at: 511 916 1024 2233 2360 511
Size: 405 108 1209 127 2395
Fragments arranged by size:
2395 1209 405 127 108
MspA1I CmG'CkG
Cuts at: 881 1355 1669 2029 3042 3287 881
Size: 474 314 360 1013 245 1838
Fragments arranged by size:
1838 1013 474 360 314 245
NaeI GCC'GGC
Cuts at: 171 2425 171
Size: 2254 1990
NarI GG'CG_CC
Cuts at: 1675 1923 1675
Size: 248 3996
NgoMIV G'CCGG_C
Cuts at: 169 2423 169
Size: 2254 1990
NspI r_CATG'y
Cuts at: 1297 1797 2704 1297
Size: 500 907 2837
Fragments arranged by size:
2837 907 500
PasI CC'CwG_GG
Cuts at: 194 194
Size: 4244
PciI A'CATG_T
Cuts at: 2700 2700
Size: 4244
PfoI T'CCnGG_A
Cuts at: 740 740
Size: 4244
PmeI GTTT'AAAC
Cuts at: 1243 1243
Size: 4244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 880 912 3383 3415 880
Size: 32 2471 32 1709
Fragments arranged by size:
2471 1709 32 32
PpuMI rG'GwC_Cy
Cuts at: 987 987
Size: 4244
PstI C_TGCA'G
Cuts at: 1291 1976 1291
Size: 685 3559
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4244
PvuII CAG'CTG
Cuts at: 1669 2029 1669
Size: 360 3884
RsrII CG'GwC_CG
Cuts at: 2439 2439
Size: 4244
SacI G_AGCT'C
Cuts at: 1258 1258
Size: 4244
SalI G'TCGA_C
Cuts at: 1281 1281
Size: 4244
SapI GCTCTTCn'nnn_
Cuts at: 404 2267 2477 404
Size: 1863 210 2171
Fragments arranged by size:
2171 1863 210
SbfI CC_TGCA'GG
Cuts at: 1291 1291
Size: 4244
ScaI AGT'ACT
Cuts at: 3761 3761
Size: 4244
SfcI C'TryA_G
Cuts at: 33 1287 1972 2965 3156 33
Size: 1254 685 993 191 1121
Fragments arranged by size:
1254 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1676 1924 1676
Size: 248 3996
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4244
SmaI CCC'GGG
Cuts at: 1266 1266
Size: 4244
SmlI C'TyrA_G
Cuts at: 820 1399 2314 2806 3068 820
Size: 579 915 492 262 1996
Fragments arranged by size:
1996 915 579 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4244
SphI G_CATG'C
Cuts at: 1297 1797 1297
Size: 500 3744
StyI C'CwwG_G
Cuts at: 134 449 1378 1732 134
Size: 315 929 354 2646
Fragments arranged by size:
2646 929 354 315
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 265 607 1061 1092 1827 1960 2419 2628 265
Size: 342 454 31 735 133 459 209 1881
Fragments arranged by size:
1881 735 459 454 342 209 133 31
TatI w'GTAC_w
Cuts at: 3759 3759
Size: 4244
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 3761 3793 745
Size: 32 2984 32 1196
Fragments arranged by size:
2984 1196 32 32
Tth111I GACn'n_nGTC
Cuts at: 2041 2041
Size: 4244
XbaI T'CTAG_A
Cuts at: 1275 1275
Size: 4244
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 642 1739 642
Size: 1097 3147
XmaI C'CCGG_G
Cuts at: 1264 1264
Size: 4244
ZraI GAC'GTC
Cuts at: 223 223
Size: 4244
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BseRI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI