Restriction map for
pF1KB6590
(Circular) (Six-base) MAPSORT of: pF1KB6590.seq Check: 1254 from: 1 to: 4331
pF1KB6590 4331 bp
With 182 enzymes: SgfI *
June 26, 2008 10:47 ..
AatII G_ACGT'C
Cuts at: 776 776
Size: 4331
AccI GT'mk_AC
Cuts at: 980 1369 980
Size: 389 3942
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 2156 2588 3335 3603 265
Size: 1891 432 747 268 993
Fragments arranged by size:
1891 993 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 985 2719 985
Size: 1734 2597
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 530 562 530
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 1636 3203 1636
Size: 1567 2764
ApaI G_GGCC'C
Cuts at: 1093 1248 1093
Size: 155 4176
ApaLI G'TGCA_C
Cuts at: 1191 3101 1191
Size: 1910 2421
ApoI r'AATT_y
Cuts at: 807 870 1335 3684 3940 807
Size: 63 465 2349 256 1198
Fragments arranged by size:
2349 1198 465 256 63
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 280 1351 280
Size: 1071 3260
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 385 441 1347 1761 2009 2044 385
Size: 56 906 414 248 35 2672
Fragments arranged by size:
2672 906 414 248 56 35
BanII G_rGCy'C
Cuts at: 163 1093 1248 1345 2375 163
Size: 930 155 97 1030 2119
Fragments arranged by size:
2119 1030 930 155 97
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 768 965 768
Size: 197 4134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1293 1327 1293
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 1252 1252
Size: 4331
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 446 1093 1195 1248 1956 2049 3105 446
Size: 647 102 53 708 93 1056 1672
Fragments arranged by size:
1672 1056 708 647 102 93 53
BmrI ACTGGGnnnn_n'
Cuts at: 354 1543 1624 1959 4103 354
Size: 1189 81 335 2144 582
Fragments arranged by size:
2144 1189 582 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 498 498
Size: 4331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 199 1507 2386 2878 3176 199
Size: 1308 879 492 298 1354
Fragments arranged by size:
1354 1308 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 181 4035 181
Size: 3854 477
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 379 1845 3882 6
Size: 373 1466 2037 455
Fragments arranged by size:
2037 1466 455 373
BsaHI Gr'CG_yC
Cuts at: 773 1762 2010 2712 4208 773
Size: 989 248 702 1496 896
Fragments arranged by size:
1496 989 896 702 248
BsaWI w'CCGG_w
Cuts at: 985 1520 2041 2719 2993 3140 3551 985
Size: 535 521 678 274 147 411 1765
Fragments arranged by size:
1765 678 535 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 310 340 530 560 733 763 310
Size: 30 190 30 173 30 3878
Fragments arranged by size:
3878 190 173 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 318 438 318
Size: 120 4211
BseYI C'CCAG_C
Cuts at: 134 819 837 854 1756 2514 3091 3587 134
Size: 685 18 17 902 758 577 496 878
Fragments arranged by size:
902 878 758 685 577 496 18 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 477 477
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1195 1345 2123 2313 3105 1195
Size: 150 778 190 792 2421
Fragments arranged by size:
2421 792 778 190 150
BsmI GAATG_Cn'
Cuts at: 227 227
Size: 4331
BsmBI CGTCTCn'nnnn_
Cuts at: 469 469
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 163 446 1093 1195 1248 1345 1956 2049 2123
Size: 283 647 102 53 97 611 93 74
Cuts at: 2123 2313 2375 3105 163
Size: 190 62 730 1389
Fragments arranged by size:
1389 730 647 611 283 190 102 97
93 74 62 53
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 1595 1897 2278 1381
Size: 214 302 381 3434
Fragments arranged by size:
3434 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 985 2329 2510 2719 985
Size: 1344 181 209 2597
Fragments arranged by size:
2597 1344 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstEII G'GTnAC_C
Cuts at: 723 723
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 351 367 351
Size: 16 4315
BstYI r'GATC_y
Cuts at: 1 206 1356 1823 1846 2181 2427 3439 3525
Size: 205 1150 467 23 335 246 1012 86
Cuts at: 3525 3537 3883 1
Size: 12 346 449
Fragments arranged by size:
1150 1012 467 449 346 335 246 205
86 23 12
BtgI C'CryG_G
Cuts at: 107 599 1211 107
Size: 492 612 3227
Fragments arranged by size:
3227 612 492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 470 1574 470
Size: 1104 3227
DraI TTT'AAA
Cuts at: 201 1330 201
Size: 1129 3202
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 266 668 1199 1916 2090 2481 2508 3853 266
Size: 402 531 717 174 391 27 1345 744
Fragments arranged by size:
1345 744 717 531 402 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 295 499 1048 2354 2564 295
Size: 204 549 1306 210 2062
Fragments arranged by size:
2062 1306 549 210 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 498 2156 2588 3335 3603 265
Size: 233 1658 432 747 268 993
Fragments arranged by size:
1658 993 747 432 268 233
EcoO109I rG'GnC_Cy
Cuts at: 1089 1090 1245 1470 1089
Size: 1 155 225 3950
Fragments arranged by size:
3950 225 155 1
EcoRI G'AATT_C
Cuts at: 807 1335 807
Size: 528 3803
EcoRV GAT'ATC
Cuts at: 1218 1218
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1765 2013 3035 4014 1765
Size: 248 1022 979 2082
Fragments arranged by size:
2082 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 763 795 2173 2203 2205 2235 763
Size: 32 1378 30 2 30 2859
Fragments arranged by size:
2859 1378 32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 268 1201 2092 268
Size: 933 891 2507
Fragments arranged by size:
2507 933 891
MslI CAynn'nnrTG
Cuts at: 598 643 2320 2447 598
Size: 45 1677 127 2482
Fragments arranged by size:
2482 1677 127 45
MspA1I CmG'CkG
Cuts at: 1442 1756 2116 3129 3374 1442
Size: 314 360 1013 245 2399
Fragments arranged by size:
2399 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 1211 107
Size: 1104 3227
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NspI r_CATG'y
Cuts at: 1384 1884 2791 1384
Size: 500 907 2924
Fragments arranged by size:
2924 907 500
PasI CC'CwG_GG
Cuts at: 1248 1248
Size: 4331
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 437 819 1212 1231 437
Size: 382 393 19 3537
Fragments arranged by size:
3537 393 382 19
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 530 562 3470 3502 530
Size: 32 2908 32 1359
Fragments arranged by size:
2908 1359 32 32
PshAI GACnn'nnGTC
Cuts at: 901 901
Size: 4331
PspOMI G'GGCC_C
Cuts at: 1089 1244 1089
Size: 155 4176
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 499 2354 2564 499
Size: 1855 210 2266
Fragments arranged by size:
2266 1855 210
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 364 3848 364
Size: 3484 847
SfcI C'TryA_G
Cuts at: 33 1173 1374 2059 3052 3243 33
Size: 1140 201 685 993 191 1121
Fragments arranged by size:
1140 1121 993 685 201 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1252 1252
Size: 4331
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SgrAI Cr'CCGG_yG
Cuts at: 985 985
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 214 1486 2401 2893 3155 214
Size: 1272 915 492 262 1390
Fragments arranged by size:
1390 1272 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
StuI AGG'CCT
Cuts at: 1039 1039
Size: 4331
StyI C'CwwG_G
Cuts at: 107 753 1040 1211 1316 1465 1819 107
Size: 646 287 171 105 149 354 2619
Fragments arranged by size:
2619 646 354 287 171 149 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 373 429 592 1242 1914 2047 2506 2715 373
Size: 56 163 650 672 133 459 209 1989
Fragments arranged by size:
1989 672 650 459 209 163 133 56
TatI w'GTAC_w
Cuts at: 362 3846 362
Size: 3484 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 555 587 599 631 818 850 1187 1219 3848
Size: 32 12 32 187 32 337 32 2629
Cuts at: 3848 3880 555
Size: 32 1006
Fragments arranged by size:
2629 1006 337 187 32 32 32 32
32 12
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1209 1245 1826 1209
Size: 36 581 3714
Fragments arranged by size:
3714 581 36
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
ZraI GAC'GTC
Cuts at: 774 774
Size: 4331
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsrGI
BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PpuMI PsiI PspXI PsrI
SacII SanDI SexAI SnaBI SrfI SspI SwaI XhoI
XmnI