Restriction map for
pF1KB6592
(Circular) (Six-base) MAPSORT of: pF1KB6592.seq Check: 4034 from: 1 to: 4331
pF1KB6592 4331 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 613 2156 2588 3335 3603 613
Size: 1543 432 747 268 1341
Fragments arranged by size:
1543 1341 747 432 268
AflIII A'CryG_T
Cuts at: 684 1683 2787 3764 684
Size: 999 1104 977 1251
Fragments arranged by size:
1251 1104 999 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AleI CACnn'nnGTG
Cuts at: 764 764
Size: 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 899
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 195 303 1636 3203 195
Size: 108 1333 1567 1323
Fragments arranged by size:
1567 1333 1323 108
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 147 494 706 946 1335 3684 3940 147
Size: 347 212 240 389 2349 256 538
Fragments arranged by size:
2349 538 389 347 256 240 212
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 1351 1351
Size: 4331
BamHI G'GATC_C
Cuts at: 893 1356 893
Size: 463 3868
BanI G'GyrC_C
Cuts at: 869 1347 1761 2009 2044 869
Size: 478 414 248 35 3156
Fragments arranged by size:
3156 478 414 248 35
BanII G_rGCy'C
Cuts at: 740 1086 1345 2375 740
Size: 346 259 1030 2696
Fragments arranged by size:
2696 1030 346 259
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 851 817
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 112 1318 112
Size: 1206 3125
BglII A'GATC_T
Cuts at: 168 1846 3883 168
Size: 1678 2037 616
Fragments arranged by size:
2037 1678 616
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 1956 2049 3105 1956
Size: 93 1056 3182
Fragments arranged by size:
3182 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1543 1624 1959 4103 1543
Size: 81 335 2144 1771
Fragments arranged by size:
2144 1771 335 81
BmtI G_CTAG'C
Cuts at: 847 847
Size: 4331
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 176 2698 176
Size: 2522 1809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 562 595 239
Size: 323 33 3975
Fragments arranged by size:
3975 323 33
Bpu10I CC'TnA_GC
Cuts at: 1106 1322 1106
Size: 216 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4035 4035
Size: 4331
BsaAI yAC'GTr
Cuts at: 685 1684 685
Size: 999 3332
BsaBI GATnn'nnATC
Cuts at: 6 584 1845 3882 6
Size: 578 1261 2037 455
Fragments arranged by size:
2037 1261 578 455
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 421 421
Size: 4331
BseYI C'CCAG_C
Cuts at: 137 1756 2514 3091 3587 137
Size: 1619 758 577 496 881
Fragments arranged by size:
1619 881 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 704 704
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 740 1086 1345 2123 2313 3105 740
Size: 346 259 778 190 792 1966
Fragments arranged by size:
1966 792 778 346 259 190
Bsp1286I G_dGCh'C
Cuts at: 740 1086 1345 1956 2049 2123 2313 2375 3105
Size: 346 259 611 93 74 190 62 730
Cuts at: 3105 740
Size: 1966
Fragments arranged by size:
1966 730 611 346 259 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 143 3507 143
Size: 3364 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 402 1381 1595 1897 2278 402
Size: 979 214 302 381 2455
Fragments arranged by size:
2455 979 381 302 214
BsrBI CCG'CTC
Cuts at: 50 155 2623 2677 4161 4326 50
Size: 105 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 105 55 54
BsrDI GCAATG_nn'
Cuts at: 266 2243 266
Size: 1977 2354
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BssSI C'ACGA_G
Cuts at: 283 1196 2602 2960 283
Size: 913 1406 358 1654
Fragments arranged by size:
1654 1406 913 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 1070 2692 1070
Size: 1622 2709
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 1174 1005
Size: 169 4162
BstYI r'GATC_y
Cuts at: 1 168 191 380 893 1094 1226 1356 1823
Size: 167 23 189 513 201 132 130 467
Cuts at: 1823 1846 2181 2427 3439 3525 3537 3883 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 513 467 449 346 335 246 201
189 167 132 130 86 23 23 12
BtgI C'CryG_G
Cuts at: 107 1304 107
Size: 1197 3134
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1574 1574
Size: 4331
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 1916 2090 2481 2508 3853 1916
Size: 174 391 27 1345 2394
Fragments arranged by size:
2394 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1126 2539 2849 2995 4141 1126
Size: 1413 310 146 1146 1316
Fragments arranged by size:
1413 1316 1146 310 146
EcoICRI GAG'CTC
Cuts at: 738 1084 1343 738
Size: 346 259 3726
Fragments arranged by size:
3726 346 259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 239 562 595 613 2156 2588 3335 3603 239
Size: 323 33 18 1543 432 747 268 967
Fragments arranged by size:
1543 967 747 432 323 268 33 18
EcoO109I rG'GnC_Cy
Cuts at: 1470 1470
Size: 4331
EcoRI G'AATT_C
Cuts at: 147 494 1335 147
Size: 347 841 3143
Fragments arranged by size:
3143 841 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1765 2013 3035 4014 1765
Size: 248 1022 979 2082
Fragments arranged by size:
2082 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1110 1142 2173 2203 2205 2235 1110
Size: 32 1031 30 2 30 3206
Fragments arranged by size:
3206 1031 32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
HindIII A'AGCT_T
Cuts at: 236 245 236
Size: 9 4322
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 764 961 1287 2320 2447 764
Size: 197 326 1033 127 2648
Fragments arranged by size:
2648 1033 326 197 127
MspA1I CmG'CkG
Cuts at: 988 1442 1756 2116 3129 3374 988
Size: 454 314 360 1013 245 1945
Fragments arranged by size:
1945 1013 454 360 314 245
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 1304 107
Size: 1197 3134
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NheI G'CTAG_C
Cuts at: 843 843
Size: 4331
NspI r_CATG'y
Cuts at: 793 1384 1884 2791 793
Size: 591 500 907 2333
Fragments arranged by size:
2333 907 591 500
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 486 486
Size: 4331
PfoI T'CCnGG_A
Cuts at: 194 194
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PmlI CAC'GTG
Cuts at: 685 685
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PshAI GACnn'nnGTC
Cuts at: 754 754
Size: 4331
PsiI TTA'TAA
Cuts at: 536 668 536
Size: 132 4199
PstI C_TGCA'G
Cuts at: 1104 1378 2063 1104
Size: 274 685 3372
Fragments arranged by size:
3372 685 274
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 988 1756 2116 988
Size: 768 360 3203
Fragments arranged by size:
3203 768 360
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 740 1086 1345 740
Size: 346 259 3726
Fragments arranged by size:
3726 346 259
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 983 1100 1374 2059 3052 3243 33
Size: 950 117 274 685 993 191 1121
Fragments arranged by size:
1121 993 950 685 274 191 117
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 1486 2401 2893 3155 1486
Size: 915 492 262 2662
Fragments arranged by size:
2662 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SspI AAT'ATT
Cuts at: 1062 1062
Size: 4331
StuI AGG'CCT
Cuts at: 781 781
Size: 4331
StyI C'CwwG_G
Cuts at: 107 371 1304 1465 1819 107
Size: 264 933 161 354 2619
Fragments arranged by size:
2619 933 354 264 161
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 521 3846 521
Size: 3325 1006
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 309 341 853 885 1226 1258 3848 3880 309
Size: 32 512 32 341 32 2590 32 760
Fragments arranged by size:
2590 760 512 341 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 912 1362 912
Size: 450 3881
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BsaXI BsiWI
BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NotI NruI NsiI PacI PasI PpuMI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI