Restriction map for
pF1KB6596
(Circular) (Six-base) MAPSORT of: pF1KB6596.seq Check: 11 from: 1 to: 4334
pF1KB6596 4334 bp
With 182 enzymes: SgfI *
November 7, 2014 16:44 ..
AccI GT'mk_AC
Cuts at: 965 1372 965
Size: 407 3927
Acc65I G'GTAC_C
Cuts at: 1350 1350
Size: 4334
AclI AA'CG_TT
Cuts at: 4174 4174
Size: 4334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 964 1287 2159 2591 3338 3606 964
Size: 323 872 432 747 268 1692
Fragments arranged by size:
1692 872 747 432 323 268
AflIII A'CryG_T
Cuts at: 1686 2790 3767 1686
Size: 1104 977 2253
Fragments arranged by size:
2253 1104 977
AgeI A'CCGG_T
Cuts at: 2722 2722
Size: 4334
AleI CACnn'nnGTG
Cuts at: 550 701 760 1189 550
Size: 151 59 429 3695
Fragments arranged by size:
3695 429 151 59
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 845 845
Size: 4334
AlwNI CAG_nnn'CTG
Cuts at: 1639 3206 1639
Size: 1567 2767
ApaI G_GGCC'C
Cuts at: 1200 1200
Size: 4334
ApaLI G'TGCA_C
Cuts at: 870 3104 870
Size: 2234 2100
ApoI r'AATT_y
Cuts at: 1338 3687 3943 1338
Size: 2349 256 1729
Fragments arranged by size:
2349 1729 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4334
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
AvaI C'yCGr_G
Cuts at: 793 1354 793
Size: 561 3773
BamHI G'GATC_C
Cuts at: 1359 1359
Size: 4334
BanI G'GyrC_C
Cuts at: 1350 1764 2012 2047 1350
Size: 414 248 35 3637
Fragments arranged by size:
3637 414 248 35
BanII G_rGCy'C
Cuts at: 997 1200 1348 2378 997
Size: 203 148 1030 2953
Fragments arranged by size:
2953 1030 203 148
BbeI G_GCGC'C
Cuts at: 1768 2016 1768
Size: 248 4086
BbsI GAAGACnn'nnnn_
Cuts at: 447 447
Size: 4334
BciVI GTATCCnnnnn_n'
Cuts at: 2232 2993 4334 2232
Size: 761 1341 2232
Fragments arranged by size:
2232 1341 761
BclI T'GATC_A
Cuts at: 1854 1854
Size: 4334
BfrBI ATG'CAT
Cuts at: 751 751
Size: 4334
BglI GCCn_nnn'nGGC
Cuts at: 349 349
Size: 4334
BglII A'GATC_T
Cuts at: 933 1849 3886 933
Size: 916 2037 1381
Fragments arranged by size:
2037 1381 916
BlpI GC'TnA_GC
Cuts at: 1446 1446
Size: 4334
Bme1580I G_kGCm'C
Cuts at: 874 1200 1959 2052 3108 874
Size: 326 759 93 1056 2100
Fragments arranged by size:
2100 1056 759 326 93
BmrI ACTGGGnnnn_n'
Cuts at: 1546 1627 1962 4106 1546
Size: 81 335 2144 1774
Fragments arranged by size:
2144 1774 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 2701 843
Size: 1858 2476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1117 1189 505
Size: 612 72 3650
Fragments arranged by size:
3650 612 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1510 2389 2881 3179 1510
Size: 879 492 298 2665
Fragments arranged by size:
2665 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 616 1165 4038 616
Size: 549 2873 912
Fragments arranged by size:
2873 912 549
BsaAI yAC'GTr
Cuts at: 1687 1687
Size: 4334
BsaBI GATnn'nnATC
Cuts at: 6 271 1848 3885 6
Size: 265 1577 2037 455
Fragments arranged by size:
2037 1577 455 265
BsaHI Gr'CG_yC
Cuts at: 1765 2013 2715 4211 1765
Size: 248 702 1496 1888
Fragments arranged by size:
1888 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1067 1523 2044 2722 2996 3143 3554 1067
Size: 456 521 678 274 147 411 1847
Fragments arranged by size:
1847 678 521 456 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 503 533 1240 1270 503
Size: 30 707 30 3567
Fragments arranged by size:
3567 707 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 792 1219 1222 1302 792
Size: 427 3 80 3824
Fragments arranged by size:
3824 427 80 3
BseYI C'CCAG_C
Cuts at: 631 1759 2517 3094 3590 631
Size: 1128 758 577 496 1375
Fragments arranged by size:
1375 1128 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1876 1922 3130 104
Size: 1772 46 1208 1308
Fragments arranged by size:
1772 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 874 997 1325 1348 2126 2316 3108 874
Size: 123 328 23 778 190 792 2100
Fragments arranged by size:
2100 792 778 328 190 123 23
BsmI GAATG_Cn'
Cuts at: 355 355
Size: 4334
Bsp1286I G_dGCh'C
Cuts at: 874 997 1200 1325 1348 1959 2052 2126 2316
Size: 123 203 125 23 611 93 74 190
Cuts at: 2316 2378 3108 874
Size: 62 730 2100
Fragments arranged by size:
2100 730 611 203 190 125 123 93
74 62 23
BspEI T'CCGG_A
Cuts at: 3554 3554
Size: 4334
BspHI T'CATG_A
Cuts at: 3510 3510
Size: 4334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 951 1384 1598 1900 2281 951
Size: 433 214 302 381 3004
Fragments arranged by size:
3004 433 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2626 2680 4164 4329 50
Size: 2576 54 1484 165 55
Fragments arranged by size:
2576 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2246 2246
Size: 4334
BsrFI r'CCGG_y
Cuts at: 1090 2332 2513 2722 1090
Size: 1242 181 209 2702
Fragments arranged by size:
2702 1242 209 181
BsrGI T'GTAC_A
Cuts at: 1004 1151 1004
Size: 147 4187
BssSI C'ACGA_G
Cuts at: 817 1201 2605 2963 817
Size: 384 1404 358 2188
Fragments arranged by size:
2188 1404 384 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1711 1711
Size: 4334
BstBI TT'CG_AA
Cuts at: 2695 2695
Size: 4334
BstEII G'GTnAC_C
Cuts at: 237 237
Size: 4334
BstXI CCAn_nnnn'nTGG
Cuts at: 552 1191 552
Size: 639 3695
BstYI r'GATC_y
Cuts at: 1 788 933 1359 1826 1849 2184 2430 3442
Size: 787 145 426 467 23 335 246 1012
Cuts at: 3442 3528 3540 3886 1
Size: 86 12 346 449
Fragments arranged by size:
1012 787 467 449 426 346 335 246
145 86 23 12
BtgI C'CryG_G
Cuts at: 107 347 107
Size: 240 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 429 2284 2465 2628 429
Size: 1855 181 163 2135
Fragments arranged by size:
2135 1855 181 163
BtsI GCAGTG_nn'
Cuts at: 1152 1577 1152
Size: 425 3909
DraI TTT'AAA
Cuts at: 1333 1333
Size: 4334
DraIII CAC_nnn'GTG
Cuts at: 1276 1276
Size: 4334
DrdI GACnn_nn'nnGTC
Cuts at: 2040 2898 2040
Size: 858 3476
EaeI y'GGCC_r
Cuts at: 503 658 971 986 1919 2093 2484 2511 3856
Size: 155 313 15 933 174 391 27 1345
Cuts at: 3856 503
Size: 981
Fragments arranged by size:
1345 981 933 391 313 174 155 27
15
EagI C'GGCC_G
Cuts at: 1919 1919
Size: 4334
EarI CTCTTCn'nnn_
Cuts at: 1272 2357 2567 1272
Size: 1085 210 3039
Fragments arranged by size:
3039 1085 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2542 2852 2998 4144 2542
Size: 310 146 1146 2732
Fragments arranged by size:
2732 1146 310 146
EcoICRI GAG'CTC
Cuts at: 995 1346 995
Size: 351 3983
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 964 1117 1189 1287 2159 2591 3338 3606
Size: 459 153 72 98 872 432 747 268
Cuts at: 3606 505
Size: 1233
Fragments arranged by size:
1233 872 747 459 432 268 153 98
72
EcoNI CCTnn'n_nnAGG
Cuts at: 492 492
Size: 4334
EcoO109I rG'GnC_Cy
Cuts at: 573 1196 1473 573
Size: 623 277 3434
Fragments arranged by size:
3434 623 277
EcoRI G'AATT_C
Cuts at: 1338 1338
Size: 4334
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1080 2353 1080
Size: 1273 3061
FspI TGC'GCA
Cuts at: 2115 2115
Size: 4334
HaeII r_GCGC'y
Cuts at: 1091 1768 2016 3038 4017 1091
Size: 677 248 1022 979 1408
Fragments arranged by size:
1408 1022 979 677 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1240 1272 2176 2206 2208 2238 1240
Size: 32 904 30 2 30 3336
Fragments arranged by size:
3336 904 32 30 30 2
HincII GTy'rAC
Cuts at: 1373 1373
Size: 4334
KasI G'GCGC_C
Cuts at: 1764 2012 1764
Size: 248 4086
KpnI G_GTAC'C
Cuts at: 1354 1354
Size: 4334
MluI A'CGCG_T
Cuts at: 3767 3767
Size: 4334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1283 3005 3189 1283
Size: 1722 184 2428
Fragments arranged by size:
2428 1722 184
MscI TGG'CCA
Cuts at: 505 660 973 988 2095 505
Size: 155 313 15 1107 2744
Fragments arranged by size:
2744 1107 313 155 15
MslI CAynn'nnrTG
Cuts at: 235 550 701 703 760 1189 2323 2450 235
Size: 315 151 2 57 429 1134 127 2119
Fragments arranged by size:
2119 1134 429 315 151 127 57 2
MspA1I CmG'CkG
Cuts at: 217 451 1445 1759 2119 3132 3377 217
Size: 234 994 314 360 1013 245 1174
Fragments arranged by size:
1174 1013 994 360 314 245 234
NaeI GCC'GGC
Cuts at: 1092 2515 1092
Size: 1423 2911
NarI GG'CG_CC
Cuts at: 1765 2013 1765
Size: 248 4086
NcoI C'CATG_G
Cuts at: 107 347 107
Size: 240 4094
NgoMIV G'CCGG_C
Cuts at: 1090 2513 1090
Size: 1423 2911
NsiI A_TGCA'T
Cuts at: 753 753
Size: 4334
NspI r_CATG'y
Cuts at: 1063 1387 1887 2794 1063
Size: 324 500 907 2603
Fragments arranged by size:
2603 907 500 324
PasI CC'CwG_GG
Cuts at: 600 1140 600
Size: 540 3794
PciI A'CATG_T
Cuts at: 2790 2790
Size: 4334
PflMI CCAn_nnn'nTGG
Cuts at: 887 980 887
Size: 93 4241
PmeI GTTT'AAAC
Cuts at: 1333 1333
Size: 4334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 4302
PpuMI rG'GwC_Cy
Cuts at: 573 573
Size: 4334
PspOMI G'GGCC_C
Cuts at: 1196 1196
Size: 4334
PstI C_TGCA'G
Cuts at: 1381 2066 1381
Size: 685 3649
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4334
PvuII CAG'CTG
Cuts at: 217 1759 2119 217
Size: 1542 360 2432
Fragments arranged by size:
2432 1542 360
RsrII CG'GwC_CG
Cuts at: 2529 2529
Size: 4334
SacI G_AGCT'C
Cuts at: 997 1348 997
Size: 351 3983
SalI G'TCGA_C
Cuts at: 1371 1371
Size: 4334
SapI GCTCTTCn'nnn_
Cuts at: 1272 2357 2567 1272
Size: 1085 210 3039
Fragments arranged by size:
3039 1085 210
SbfI CC_TGCA'GG
Cuts at: 1381 1381
Size: 4334
ScaI AGT'ACT
Cuts at: 3851 3851
Size: 4334
SfcI C'TryA_G
Cuts at: 33 1377 2062 3055 3246 33
Size: 1344 685 993 191 1121
Fragments arranged by size:
1344 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1766 2014 1766
Size: 248 4086
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4334
SgrAI Cr'CCGG_yG
Cuts at: 1090 1090
Size: 4334
SmaI CCC'GGG
Cuts at: 795 1356 795
Size: 561 3773
SmlI C'TyrA_G
Cuts at: 1489 2404 2896 3158 1489
Size: 915 492 262 2665
Fragments arranged by size:
2665 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4334
SphI G_CATG'C
Cuts at: 1063 1387 1887 1063
Size: 324 500 3510
Fragments arranged by size:
3510 500 324
StuI AGG'CCT
Cuts at: 343 343
Size: 4334
StyI C'CwwG_G
Cuts at: 107 347 1226 1468 1822 107
Size: 240 879 242 354 2619
Fragments arranged by size:
2619 879 354 242 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1917 2050 2509 2718 1917
Size: 133 459 209 3533
Fragments arranged by size:
3533 459 209 133
TatI w'GTAC_w
Cuts at: 1004 1151 3849 1004
Size: 147 2698 1489
Fragments arranged by size:
2698 1489 147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3851 3883 3851
Size: 32 4302
Tth111I GACn'n_nGTC
Cuts at: 2131 2131
Size: 4334
XbaI T'CTAG_A
Cuts at: 1365 1365
Size: 4334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 480 840 981 1185 1829 480
Size: 360 141 204 644 2985
Fragments arranged by size:
2985 644 360 204 141
XmaI C'CCGG_G
Cuts at: 793 1354 793
Size: 561 3773
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PasI PciI PflMI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsgI BsiWI
BsmBI BssHII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PfoI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI