(Circular) (Six-base) MAPSORT of: pF1KB6603.seq Check: 1670 from: 1 to: 4337 pF1KB6603 4337 bp With 182 enzymes: SgfI * October 7, 2009 14:21 .. AccI GT'mk_AC Cuts at: 1375 1375 Size: 4337 Acc65I G'GTAC_C Cuts at: 246 1353 246 Size: 1107 3230 AclI AA'CG_TT Cuts at: 4177 4177 Size: 4337 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 422 1072 2162 2594 3341 3609 422 Size: 650 1090 432 747 268 1150 Fragments arranged by size: 1150 1090 747 650 432 268 AflIII A'CryG_T Cuts at: 1689 2793 3770 1689 Size: 1104 977 2256 Fragments arranged by size: 2256 1104 977 AgeI A'CCGG_T Cuts at: 2725 2725 Size: 4337 AlwNI CAG_nnn'CTG Cuts at: 1642 3209 1642 Size: 1567 2770 ApaLI G'TGCA_C Cuts at: 3107 3107 Size: 4337 ApoI r'AATT_y Cuts at: 268 604 811 1195 1341 3690 3946 268 Size: 336 207 384 146 2349 256 659 Fragments arranged by size: 2349 659 384 336 256 207 146 AseI AT'TA_AT Cuts at: 20 20 Size: 4337 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4337 AvaI C'yCGr_G Cuts at: 1357 1357 Size: 4337 BamHI G'GATC_C Cuts at: 1362 1362 Size: 4337 BanI G'GyrC_C Cuts at: 246 1353 1767 2015 2050 246 Size: 1107 414 248 35 2533 Fragments arranged by size: 2533 1107 414 248 35 BanII G_rGCy'C Cuts at: 1351 2381 1351 Size: 1030 3307 BbeI G_GCGC'C Cuts at: 1771 2019 1771 Size: 248 4089 BbsI GAAGACnn'nnnn_ Cuts at: 1073 1073 Size: 4337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 655 689 655 Size: 34 4303 BciVI GTATCCnnnnn_n' Cuts at: 2235 2996 4337 2235 Size: 761 1341 2235 Fragments arranged by size: 2235 1341 761 BclI T'GATC_A Cuts at: 831 1857 831 Size: 1026 3311 BglI GCCn_nnn'nGGC Cuts at: 148 148 Size: 4337 BglII A'GATC_T Cuts at: 439 1852 3889 439 Size: 1413 2037 887 Fragments arranged by size: 2037 1413 887 BlpI GC'TnA_GC Cuts at: 1449 1449 Size: 4337 Bme1580I G_kGCm'C Cuts at: 1962 2055 3111 1962 Size: 93 1056 3188 Fragments arranged by size: 3188 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 1549 1630 1965 4109 1549 Size: 81 335 2144 1777 Fragments arranged by size: 2144 1777 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2704 2704 Size: 4337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 166 1343 166 Size: 1177 3160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1513 2392 2884 3182 1513 Size: 879 492 298 2668 Fragments arranged by size: 2668 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 386 607 1319 4041 386 Size: 221 712 2722 682 Fragments arranged by size: 2722 712 682 221 BsaAI yAC'GTr Cuts at: 1690 1690 Size: 4337 BsaBI GATnn'nnATC Cuts at: 6 1851 3888 6 Size: 1845 2037 455 Fragments arranged by size: 2037 1845 455 BsaHI Gr'CG_yC Cuts at: 1768 2016 2718 4214 1768 Size: 248 702 1496 1891 Fragments arranged by size: 1891 1496 702 248 BsaWI w'CCGG_w Cuts at: 1526 2047 2725 2999 3146 3557 1526 Size: 521 678 274 147 411 2306 Fragments arranged by size: 2306 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1316 1346 1316 Size: 30 4307 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 920 920 Size: 4337 BseYI C'CCAG_C Cuts at: 242 1145 1762 2520 3097 3593 242 Size: 903 617 758 577 496 986 Fragments arranged by size: 986 903 758 617 577 496 BsiEI CG_ry'CG Cuts at: 104 1879 1925 3133 104 Size: 1775 46 1208 1308 Fragments arranged by size: 1775 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1351 2129 2319 3111 1351 Size: 778 190 792 2577 Fragments arranged by size: 2577 792 778 190 Bsp1286I G_dGCh'C Cuts at: 1351 1962 2055 2129 2319 2381 3111 1351 Size: 611 93 74 190 62 730 2577 Fragments arranged by size: 2577 730 611 190 93 74 62 BspEI T'CCGG_A Cuts at: 3557 3557 Size: 4337 BspHI T'CATG_A Cuts at: 3513 3513 Size: 4337 BspMI ACCTGCnnnn'nnnn_ Cuts at: 653 1296 1387 1601 1903 2284 653 Size: 643 91 214 302 381 2706 Fragments arranged by size: 2706 643 381 302 214 91 BsrBI CCG'CTC Cuts at: 50 2629 2683 4167 4332 50 Size: 2579 54 1484 165 55 Fragments arranged by size: 2579 1484 165 55 54 BsrDI GCAATG_nn' Cuts at: 541 2249 541 Size: 1708 2629 BsrFI r'CCGG_y Cuts at: 2335 2516 2725 2335 Size: 181 209 3947 Fragments arranged by size: 3947 209 181 BssSI C'ACGA_G Cuts at: 2608 2966 2608 Size: 358 3979 BstAPI GCAn_nnn'nTGC Cuts at: 1714 1714 Size: 4337 BstBI TT'CG_AA Cuts at: 2698 2698 Size: 4337 BstEII G'GTnAC_C Cuts at: 982 982 Size: 4337 BstYI r'GATC_y Cuts at: 1 439 1362 1829 1852 2187 2433 3445 3531 Size: 438 923 467 23 335 246 1012 86 Cuts at: 3531 3543 3889 1 Size: 12 346 449 Fragments arranged by size: 1012 923 467 449 438 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 199 107 Size: 92 4245 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2287 2468 2631 2287 Size: 181 163 3993 Fragments arranged by size: 3993 181 163 BtsI GCAGTG_nn' Cuts at: 1580 1580 Size: 4337 DraI TTT'AAA Cuts at: 933 1116 1336 933 Size: 183 220 3934 Fragments arranged by size: 3934 220 183 DrdI GACnn_nn'nnGTC Cuts at: 2043 2901 2043 Size: 858 3479 EaeI y'GGCC_r Cuts at: 621 1922 2096 2487 2514 3859 621 Size: 1301 174 391 27 1345 1099 Fragments arranged by size: 1345 1301 1099 391 174 27 EagI C'GGCC_G Cuts at: 1922 1922 Size: 4337 EarI CTCTTCn'nnn_ Cuts at: 2360 2570 2360 Size: 210 4127 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2545 2855 3001 4147 2545 Size: 310 146 1146 2735 Fragments arranged by size: 2735 1146 310 146 EcoICRI GAG'CTC Cuts at: 1349 1349 Size: 4337 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 166 422 1072 1343 2162 2594 3341 3609 166 Size: 256 650 271 819 432 747 268 894 Fragments arranged by size: 894 819 747 650 432 271 268 256 EcoO109I rG'GnC_Cy Cuts at: 1476 1476 Size: 4337 EcoRI G'AATT_C Cuts at: 1341 1341 Size: 4337 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 4337 FspI TGC'GCA Cuts at: 2118 2118 Size: 4337 HaeII r_GCGC'y Cuts at: 1771 2019 3041 4020 1771 Size: 248 1022 979 2088 Fragments arranged by size: 2088 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 2179 2209 2211 2241 2179 Size: 30 2 30 4275 Fragments arranged by size: 4275 30 30 2 HincII GTy'rAC Cuts at: 1376 1376 Size: 4337 KasI G'GCGC_C Cuts at: 1767 2015 1767 Size: 248 4089 KpnI G_GTAC'C Cuts at: 250 1357 250 Size: 1107 3230 MluI A'CGCG_T Cuts at: 3770 3770 Size: 4337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3008 3192 3008 Size: 184 4153 MscI TGG'CCA Cuts at: 623 2098 623 Size: 1475 2862 MslI CAynn'nnrTG Cuts at: 2326 2453 2326 Size: 127 4210 MspA1I CmG'CkG Cuts at: 1448 1762 2122 3135 3380 1448 Size: 314 360 1013 245 2405 Fragments arranged by size: 2405 1013 360 314 245 NaeI GCC'GGC Cuts at: 2518 2518 Size: 4337 NarI GG'CG_CC Cuts at: 1768 2016 1768 Size: 248 4089 NcoI C'CATG_G Cuts at: 107 107 Size: 4337 NgoMIV G'CCGG_C Cuts at: 2516 2516 Size: 4337 NspI r_CATG'y Cuts at: 1390 1890 2797 1390 Size: 500 907 2930 Fragments arranged by size: 2930 907 500 PciI A'CATG_T Cuts at: 2793 2793 Size: 4337 PmeI GTTT'AAAC Cuts at: 1336 1336 Size: 4337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 3476 Size: 32 4305 PstI C_TGCA'G Cuts at: 1384 2069 1384 Size: 685 3652 PvuI CG_AT'CG Cuts at: 104 104 Size: 4337 PvuII CAG'CTG Cuts at: 1762 2122 1762 Size: 360 3977 RsrII CG'GwC_CG Cuts at: 2532 2532 Size: 4337 SacI G_AGCT'C Cuts at: 1351 1351 Size: 4337 SalI G'TCGA_C Cuts at: 1374 1374 Size: 4337 SapI GCTCTTCn'nnn_ Cuts at: 2360 2570 2360 Size: 210 4127 SbfI CC_TGCA'GG Cuts at: 1384 1384 Size: 4337 ScaI AGT'ACT Cuts at: 518 1022 3854 518 Size: 504 2832 1001 Fragments arranged by size: 2832 1001 504 SexAI A'CCwGG_T Cuts at: 1328 1328 Size: 4337 SfcI C'TryA_G Cuts at: 33 840 1380 2065 3058 3249 33 Size: 807 540 685 993 191 1121 Fragments arranged by size: 1121 993 807 685 540 191 SfoI GGC'GCC Cuts at: 1769 2017 1769 Size: 248 4089 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4337 SmaI CCC'GGG Cuts at: 1359 1359 Size: 4337 SmlI C'TyrA_G Cuts at: 1492 2407 2899 3161 1492 Size: 915 492 262 2668 Fragments arranged by size: 2668 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4337 SphI G_CATG'C Cuts at: 1390 1890 1390 Size: 500 3837 SspI AAT'ATT Cuts at: 525 525 Size: 4337 StyI C'CwwG_G Cuts at: 107 332 944 1471 1825 107 Size: 225 612 527 354 2619 Fragments arranged by size: 2619 612 527 354 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1920 2053 2512 2721 1920 Size: 133 459 209 3536 Fragments arranged by size: 3536 459 209 133 TatI w'GTAC_w Cuts at: 516 629 1020 1170 3852 516 Size: 113 391 150 2682 1001 Fragments arranged by size: 2682 1001 391 150 113 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3854 3886 3854 Size: 32 4305 Tth111I GACn'n_nGTC Cuts at: 898 2134 898 Size: 1236 3101 XbaI T'CTAG_A Cuts at: 783 1368 783 Size: 585 3752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 951 1153 1832 951 Size: 202 679 3456 Fragments arranged by size: 3456 679 202 XmaI C'CCGG_G Cuts at: 1357 1357 Size: 4337 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI