Restriction map for pF1KB6603
 (Circular) (Six-base) MAPSORT of: pF1KB6603.seq  Check: 1670  from: 1  to: 4337



pF1KB6603  4337 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:21  ..

AccI GT'mk_AC

Cuts at:   1375    1375 
   Size:       4337
 
Acc65I G'GTAC_C

Cuts at:    246    1353     246 
   Size:       1107    3230
 
AclI AA'CG_TT

Cuts at:   4177    4177 
   Size:       4337
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    422    1072    2162    2594    3341    3609     422 
   Size:        650    1090     432     747     268    1150

  Fragments arranged by size: 

                1150    1090     747     650     432     268
 
AflIII A'CryG_T

Cuts at:   1689    2793    3770    1689 
   Size:       1104     977    2256

  Fragments arranged by size: 

                2256    1104     977
 
AgeI A'CCGG_T

Cuts at:   2725    2725 
   Size:       4337
 
AlwNI CAG_nnn'CTG

Cuts at:   1642    3209    1642 
   Size:       1567    2770
 
ApaLI G'TGCA_C

Cuts at:   3107    3107 
   Size:       4337
 
ApoI r'AATT_y

Cuts at:    268     604     811    1195    1341    3690    3946     268 
   Size:        336     207     384     146    2349     256     659

  Fragments arranged by size: 

                2349     659     384     336     256     207     146
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4337
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4337
 
AvaI C'yCGr_G

Cuts at:   1357    1357 
   Size:       4337
 
BamHI G'GATC_C

Cuts at:   1362    1362 
   Size:       4337
 
BanI G'GyrC_C

Cuts at:    246    1353    1767    2015    2050     246 
   Size:       1107     414     248      35    2533

  Fragments arranged by size: 

                2533    1107     414     248      35
 
BanII G_rGCy'C

Cuts at:   1351    2381    1351 
   Size:       1030    3307
 
BbeI G_GCGC'C

Cuts at:   1771    2019    1771 
   Size:        248    4089
 
BbsI GAAGACnn'nnnn_

Cuts at:   1073    1073 
   Size:       4337
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    655     689     655 
   Size:         34    4303
 
BciVI GTATCCnnnnn_n'

Cuts at:   2235    2996    4337    2235 
   Size:        761    1341    2235

  Fragments arranged by size: 

                2235    1341     761
 
BclI T'GATC_A

Cuts at:    831    1857     831 
   Size:       1026    3311
 
BglI GCCn_nnn'nGGC

Cuts at:    148     148 
   Size:       4337
 
BglII A'GATC_T

Cuts at:    439    1852    3889     439 
   Size:       1413    2037     887

  Fragments arranged by size: 

                2037    1413     887
 
BlpI GC'TnA_GC

Cuts at:   1449    1449 
   Size:       4337
 
Bme1580I G_kGCm'C

Cuts at:   1962    2055    3111    1962 
   Size:         93    1056    3188

  Fragments arranged by size: 

                3188    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1549    1630    1965    4109    1549 
   Size:         81     335    2144    1777

  Fragments arranged by size: 

                2144    1777     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2704    2704 
   Size:       4337
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    166    1343     166 
   Size:       1177    3160
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1513    2392    2884    3182    1513 
   Size:        879     492     298    2668

  Fragments arranged by size: 

                2668     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    386     607    1319    4041     386 
   Size:        221     712    2722     682

  Fragments arranged by size: 

                2722     712     682     221
 
BsaAI yAC'GTr

Cuts at:   1690    1690 
   Size:       4337
 
BsaBI GATnn'nnATC

Cuts at:      6    1851    3888       6 
   Size:       1845    2037     455

  Fragments arranged by size: 

                2037    1845     455
 
BsaHI Gr'CG_yC

Cuts at:   1768    2016    2718    4214    1768 
   Size:        248     702    1496    1891

  Fragments arranged by size: 

                1891    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1526    2047    2725    2999    3146    3557    1526 
   Size:        521     678     274     147     411    2306

  Fragments arranged by size: 

                2306     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1316    1346    1316 
   Size:         30    4307
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    920     920 
   Size:       4337
 
BseYI C'CCAG_C

Cuts at:    242    1145    1762    2520    3097    3593     242 
   Size:        903     617     758     577     496     986

  Fragments arranged by size: 

                 986     903     758     617     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1879    1925    3133     104 
   Size:       1775      46    1208    1308

  Fragments arranged by size: 

                1775    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1351    2129    2319    3111    1351 
   Size:        778     190     792    2577

  Fragments arranged by size: 

                2577     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:   1351    1962    2055    2129    2319    2381    3111    1351 
   Size:        611      93      74     190      62     730    2577

  Fragments arranged by size: 

                2577     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3557    3557 
   Size:       4337
 
BspHI T'CATG_A

Cuts at:   3513    3513 
   Size:       4337
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    653    1296    1387    1601    1903    2284     653 
   Size:        643      91     214     302     381    2706

  Fragments arranged by size: 

                2706     643     381     302     214      91
 
BsrBI CCG'CTC

Cuts at:     50    2629    2683    4167    4332      50 
   Size:       2579      54    1484     165      55

  Fragments arranged by size: 

                2579    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    541    2249     541 
   Size:       1708    2629
 
BsrFI r'CCGG_y

Cuts at:   2335    2516    2725    2335 
   Size:        181     209    3947

  Fragments arranged by size: 

                3947     209     181
 
BssSI C'ACGA_G

Cuts at:   2608    2966    2608 
   Size:        358    3979
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1714    1714 
   Size:       4337
 
BstBI TT'CG_AA

Cuts at:   2698    2698 
   Size:       4337
 
BstEII G'GTnAC_C

Cuts at:    982     982 
   Size:       4337
 
BstYI r'GATC_y

Cuts at:      1     439    1362    1829    1852    2187    2433    3445    3531 
   Size:        438     923     467      23     335     246    1012      86

Cuts at:   3531    3543    3889       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     923     467     449     438     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     199     107 
   Size:         92    4245
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2287    2468    2631    2287 
   Size:        181     163    3993

  Fragments arranged by size: 

                3993     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1580    1580 
   Size:       4337
 
DraI TTT'AAA

Cuts at:    933    1116    1336     933 
   Size:        183     220    3934

  Fragments arranged by size: 

                3934     220     183
 
DrdI GACnn_nn'nnGTC

Cuts at:   2043    2901    2043 
   Size:        858    3479
 
EaeI y'GGCC_r

Cuts at:    621    1922    2096    2487    2514    3859     621 
   Size:       1301     174     391      27    1345    1099

  Fragments arranged by size: 

                1345    1301    1099     391     174      27
 
EagI C'GGCC_G

Cuts at:   1922    1922 
   Size:       4337
 
EarI CTCTTCn'nnn_

Cuts at:   2360    2570    2360 
   Size:        210    4127
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2545    2855    3001    4147    2545 
   Size:        310     146    1146    2735

  Fragments arranged by size: 

                2735    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1349    1349 
   Size:       4337
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    166     422    1072    1343    2162    2594    3341    3609     166 
   Size:        256     650     271     819     432     747     268     894

  Fragments arranged by size: 

                 894     819     747     650     432     271     268     256
            
 
EcoO109I rG'GnC_Cy

Cuts at:   1476    1476 
   Size:       4337
 
EcoRI G'AATT_C

Cuts at:   1341    1341 
   Size:       4337
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2356    2356 
   Size:       4337
 
FspI TGC'GCA

Cuts at:   2118    2118 
   Size:       4337
 
HaeII r_GCGC'y

Cuts at:   1771    2019    3041    4020    1771 
   Size:        248    1022     979    2088

  Fragments arranged by size: 

                2088    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   2179    2209    2211    2241    2179 
   Size:         30       2      30    4275

  Fragments arranged by size: 

                4275      30      30       2
 
HincII GTy'rAC

Cuts at:   1376    1376 
   Size:       4337
 
KasI G'GCGC_C

Cuts at:   1767    2015    1767 
   Size:        248    4089
 
KpnI G_GTAC'C

Cuts at:    250    1357     250 
   Size:       1107    3230
 
MluI A'CGCG_T

Cuts at:   3770    3770 
   Size:       4337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3008    3192    3008 
   Size:        184    4153
 
MscI TGG'CCA

Cuts at:    623    2098     623 
   Size:       1475    2862
 
MslI CAynn'nnrTG

Cuts at:   2326    2453    2326 
   Size:        127    4210
 
MspA1I CmG'CkG

Cuts at:   1448    1762    2122    3135    3380    1448 
   Size:        314     360    1013     245    2405

  Fragments arranged by size: 

                2405    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   2518    2518 
   Size:       4337
 
NarI GG'CG_CC

Cuts at:   1768    2016    1768 
   Size:        248    4089
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4337
 
NgoMIV G'CCGG_C

Cuts at:   2516    2516 
   Size:       4337
 
NspI r_CATG'y

Cuts at:   1390    1890    2797    1390 
   Size:        500     907    2930

  Fragments arranged by size: 

                2930     907     500
 
PciI A'CATG_T

Cuts at:   2793    2793 
   Size:       4337
 
PmeI GTTT'AAAC

Cuts at:   1336    1336 
   Size:       4337
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3476    3508    3476 
   Size:         32    4305
 
PstI C_TGCA'G

Cuts at:   1384    2069    1384 
   Size:        685    3652
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4337
 
PvuII CAG'CTG

Cuts at:   1762    2122    1762 
   Size:        360    3977
 
RsrII CG'GwC_CG

Cuts at:   2532    2532 
   Size:       4337
 
SacI G_AGCT'C

Cuts at:   1351    1351 
   Size:       4337
 
SalI G'TCGA_C

Cuts at:   1374    1374 
   Size:       4337
 
SapI GCTCTTCn'nnn_

Cuts at:   2360    2570    2360 
   Size:        210    4127
 
SbfI CC_TGCA'GG

Cuts at:   1384    1384 
   Size:       4337
 
ScaI AGT'ACT

Cuts at:    518    1022    3854     518 
   Size:        504    2832    1001

  Fragments arranged by size: 

                2832    1001     504
 
SexAI A'CCwGG_T

Cuts at:   1328    1328 
   Size:       4337
 
SfcI C'TryA_G

Cuts at:     33     840    1380    2065    3058    3249      33 
   Size:        807     540     685     993     191    1121

  Fragments arranged by size: 

                1121     993     807     685     540     191
 
SfoI GGC'GCC

Cuts at:   1769    2017    1769 
   Size:        248    4089
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4337
 
SmaI CCC'GGG

Cuts at:   1359    1359 
   Size:       4337
 
SmlI C'TyrA_G

Cuts at:   1492    2407    2899    3161    1492 
   Size:        915     492     262    2668

  Fragments arranged by size: 

                2668     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4337
 
SphI G_CATG'C

Cuts at:   1390    1890    1390 
   Size:        500    3837
 
SspI AAT'ATT

Cuts at:    525     525 
   Size:       4337
 
StyI C'CwwG_G

Cuts at:    107     332     944    1471    1825     107 
   Size:        225     612     527     354    2619

  Fragments arranged by size: 

                2619     612     527     354     225
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1920    2053    2512    2721    1920 
   Size:        133     459     209    3536

  Fragments arranged by size: 

                3536     459     209     133
 
TatI w'GTAC_w

Cuts at:    516     629    1020    1170    3852     516 
   Size:        113     391     150    2682    1001

  Fragments arranged by size: 

                2682    1001     391     150     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3854    3886    3854 
   Size:         32    4305
 
Tth111I GACn'n_nGTC

Cuts at:    898    2134     898 
   Size:       1236    3101
 
XbaI T'CTAG_A

Cuts at:    783    1368     783 
   Size:        585    3752
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    951    1153    1832     951 
   Size:        202     679    3456

  Fragments arranged by size: 

                3456     679     202
 
XmaI C'CCGG_G

Cuts at:   1357    1357 
   Size:       4337
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
   BstEII    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI
   Bpu10I     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BssHII    BstXI
  BstZ17I   Bsu36I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI
    SnaBI     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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