Restriction map for
pF1KB6603
(Circular) (Six-base) MAPSORT of: pF1KB6603.seq Check: 1670 from: 1 to: 4337
pF1KB6603 4337 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1375 1375
Size: 4337
Acc65I G'GTAC_C
Cuts at: 246 1353 246
Size: 1107 3230
AclI AA'CG_TT
Cuts at: 4177 4177
Size: 4337
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 422 1072 2162 2594 3341 3609 422
Size: 650 1090 432 747 268 1150
Fragments arranged by size:
1150 1090 747 650 432 268
AflIII A'CryG_T
Cuts at: 1689 2793 3770 1689
Size: 1104 977 2256
Fragments arranged by size:
2256 1104 977
AgeI A'CCGG_T
Cuts at: 2725 2725
Size: 4337
AlwNI CAG_nnn'CTG
Cuts at: 1642 3209 1642
Size: 1567 2770
ApaLI G'TGCA_C
Cuts at: 3107 3107
Size: 4337
ApoI r'AATT_y
Cuts at: 268 604 811 1195 1341 3690 3946 268
Size: 336 207 384 146 2349 256 659
Fragments arranged by size:
2349 659 384 336 256 207 146
AseI AT'TA_AT
Cuts at: 20 20
Size: 4337
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4337
AvaI C'yCGr_G
Cuts at: 1357 1357
Size: 4337
BamHI G'GATC_C
Cuts at: 1362 1362
Size: 4337
BanI G'GyrC_C
Cuts at: 246 1353 1767 2015 2050 246
Size: 1107 414 248 35 2533
Fragments arranged by size:
2533 1107 414 248 35
BanII G_rGCy'C
Cuts at: 1351 2381 1351
Size: 1030 3307
BbeI G_GCGC'C
Cuts at: 1771 2019 1771
Size: 248 4089
BbsI GAAGACnn'nnnn_
Cuts at: 1073 1073
Size: 4337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 655 689 655
Size: 34 4303
BciVI GTATCCnnnnn_n'
Cuts at: 2235 2996 4337 2235
Size: 761 1341 2235
Fragments arranged by size:
2235 1341 761
BclI T'GATC_A
Cuts at: 831 1857 831
Size: 1026 3311
BglI GCCn_nnn'nGGC
Cuts at: 148 148
Size: 4337
BglII A'GATC_T
Cuts at: 439 1852 3889 439
Size: 1413 2037 887
Fragments arranged by size:
2037 1413 887
BlpI GC'TnA_GC
Cuts at: 1449 1449
Size: 4337
Bme1580I G_kGCm'C
Cuts at: 1962 2055 3111 1962
Size: 93 1056 3188
Fragments arranged by size:
3188 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1549 1630 1965 4109 1549
Size: 81 335 2144 1777
Fragments arranged by size:
2144 1777 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2704 2704
Size: 4337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 1343 166
Size: 1177 3160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1513 2392 2884 3182 1513
Size: 879 492 298 2668
Fragments arranged by size:
2668 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 386 607 1319 4041 386
Size: 221 712 2722 682
Fragments arranged by size:
2722 712 682 221
BsaAI yAC'GTr
Cuts at: 1690 1690
Size: 4337
BsaBI GATnn'nnATC
Cuts at: 6 1851 3888 6
Size: 1845 2037 455
Fragments arranged by size:
2037 1845 455
BsaHI Gr'CG_yC
Cuts at: 1768 2016 2718 4214 1768
Size: 248 702 1496 1891
Fragments arranged by size:
1891 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1526 2047 2725 2999 3146 3557 1526
Size: 521 678 274 147 411 2306
Fragments arranged by size:
2306 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1316 1346 1316
Size: 30 4307
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 920 920
Size: 4337
BseYI C'CCAG_C
Cuts at: 242 1145 1762 2520 3097 3593 242
Size: 903 617 758 577 496 986
Fragments arranged by size:
986 903 758 617 577 496
BsiEI CG_ry'CG
Cuts at: 104 1879 1925 3133 104
Size: 1775 46 1208 1308
Fragments arranged by size:
1775 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1351 2129 2319 3111 1351
Size: 778 190 792 2577
Fragments arranged by size:
2577 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1351 1962 2055 2129 2319 2381 3111 1351
Size: 611 93 74 190 62 730 2577
Fragments arranged by size:
2577 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3557 3557
Size: 4337
BspHI T'CATG_A
Cuts at: 3513 3513
Size: 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 653 1296 1387 1601 1903 2284 653
Size: 643 91 214 302 381 2706
Fragments arranged by size:
2706 643 381 302 214 91
BsrBI CCG'CTC
Cuts at: 50 2629 2683 4167 4332 50
Size: 2579 54 1484 165 55
Fragments arranged by size:
2579 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 541 2249 541
Size: 1708 2629
BsrFI r'CCGG_y
Cuts at: 2335 2516 2725 2335
Size: 181 209 3947
Fragments arranged by size:
3947 209 181
BssSI C'ACGA_G
Cuts at: 2608 2966 2608
Size: 358 3979
BstAPI GCAn_nnn'nTGC
Cuts at: 1714 1714
Size: 4337
BstBI TT'CG_AA
Cuts at: 2698 2698
Size: 4337
BstEII G'GTnAC_C
Cuts at: 982 982
Size: 4337
BstYI r'GATC_y
Cuts at: 1 439 1362 1829 1852 2187 2433 3445 3531
Size: 438 923 467 23 335 246 1012 86
Cuts at: 3531 3543 3889 1
Size: 12 346 449
Fragments arranged by size:
1012 923 467 449 438 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 199 107
Size: 92 4245
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2287 2468 2631 2287
Size: 181 163 3993
Fragments arranged by size:
3993 181 163
BtsI GCAGTG_nn'
Cuts at: 1580 1580
Size: 4337
DraI TTT'AAA
Cuts at: 933 1116 1336 933
Size: 183 220 3934
Fragments arranged by size:
3934 220 183
DrdI GACnn_nn'nnGTC
Cuts at: 2043 2901 2043
Size: 858 3479
EaeI y'GGCC_r
Cuts at: 621 1922 2096 2487 2514 3859 621
Size: 1301 174 391 27 1345 1099
Fragments arranged by size:
1345 1301 1099 391 174 27
EagI C'GGCC_G
Cuts at: 1922 1922
Size: 4337
EarI CTCTTCn'nnn_
Cuts at: 2360 2570 2360
Size: 210 4127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2545 2855 3001 4147 2545
Size: 310 146 1146 2735
Fragments arranged by size:
2735 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1349 1349
Size: 4337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 166 422 1072 1343 2162 2594 3341 3609 166
Size: 256 650 271 819 432 747 268 894
Fragments arranged by size:
894 819 747 650 432 271 268 256
EcoO109I rG'GnC_Cy
Cuts at: 1476 1476
Size: 4337
EcoRI G'AATT_C
Cuts at: 1341 1341
Size: 4337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 4337
FspI TGC'GCA
Cuts at: 2118 2118
Size: 4337
HaeII r_GCGC'y
Cuts at: 1771 2019 3041 4020 1771
Size: 248 1022 979 2088
Fragments arranged by size:
2088 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2179 2209 2211 2241 2179
Size: 30 2 30 4275
Fragments arranged by size:
4275 30 30 2
HincII GTy'rAC
Cuts at: 1376 1376
Size: 4337
KasI G'GCGC_C
Cuts at: 1767 2015 1767
Size: 248 4089
KpnI G_GTAC'C
Cuts at: 250 1357 250
Size: 1107 3230
MluI A'CGCG_T
Cuts at: 3770 3770
Size: 4337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3008 3192 3008
Size: 184 4153
MscI TGG'CCA
Cuts at: 623 2098 623
Size: 1475 2862
MslI CAynn'nnrTG
Cuts at: 2326 2453 2326
Size: 127 4210
MspA1I CmG'CkG
Cuts at: 1448 1762 2122 3135 3380 1448
Size: 314 360 1013 245 2405
Fragments arranged by size:
2405 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2518 2518
Size: 4337
NarI GG'CG_CC
Cuts at: 1768 2016 1768
Size: 248 4089
NcoI C'CATG_G
Cuts at: 107 107
Size: 4337
NgoMIV G'CCGG_C
Cuts at: 2516 2516
Size: 4337
NspI r_CATG'y
Cuts at: 1390 1890 2797 1390
Size: 500 907 2930
Fragments arranged by size:
2930 907 500
PciI A'CATG_T
Cuts at: 2793 2793
Size: 4337
PmeI GTTT'AAAC
Cuts at: 1336 1336
Size: 4337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3476 3508 3476
Size: 32 4305
PstI C_TGCA'G
Cuts at: 1384 2069 1384
Size: 685 3652
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4337
PvuII CAG'CTG
Cuts at: 1762 2122 1762
Size: 360 3977
RsrII CG'GwC_CG
Cuts at: 2532 2532
Size: 4337
SacI G_AGCT'C
Cuts at: 1351 1351
Size: 4337
SalI G'TCGA_C
Cuts at: 1374 1374
Size: 4337
SapI GCTCTTCn'nnn_
Cuts at: 2360 2570 2360
Size: 210 4127
SbfI CC_TGCA'GG
Cuts at: 1384 1384
Size: 4337
ScaI AGT'ACT
Cuts at: 518 1022 3854 518
Size: 504 2832 1001
Fragments arranged by size:
2832 1001 504
SexAI A'CCwGG_T
Cuts at: 1328 1328
Size: 4337
SfcI C'TryA_G
Cuts at: 33 840 1380 2065 3058 3249 33
Size: 807 540 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 540 191
SfoI GGC'GCC
Cuts at: 1769 2017 1769
Size: 248 4089
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4337
SmaI CCC'GGG
Cuts at: 1359 1359
Size: 4337
SmlI C'TyrA_G
Cuts at: 1492 2407 2899 3161 1492
Size: 915 492 262 2668
Fragments arranged by size:
2668 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4337
SphI G_CATG'C
Cuts at: 1390 1890 1390
Size: 500 3837
SspI AAT'ATT
Cuts at: 525 525
Size: 4337
StyI C'CwwG_G
Cuts at: 107 332 944 1471 1825 107
Size: 225 612 527 354 2619
Fragments arranged by size:
2619 612 527 354 225
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1920 2053 2512 2721 1920
Size: 133 459 209 3536
Fragments arranged by size:
3536 459 209 133
TatI w'GTAC_w
Cuts at: 516 629 1020 1170 3852 516
Size: 113 391 150 2682 1001
Fragments arranged by size:
2682 1001 391 150 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3854 3886 3854
Size: 32 4305
Tth111I GACn'n_nGTC
Cuts at: 898 2134 898
Size: 1236 3101
XbaI T'CTAG_A
Cuts at: 783 1368 783
Size: 585 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 951 1153 1832 951
Size: 202 679 3456
Fragments arranged by size:
3456 679 202
XmaI C'CCGG_G
Cuts at: 1357 1357
Size: 4337
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI
Bpu10I BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI XmnI ZraI