Restriction map for
pF1KB6605
(Circular) (Six-base) MAPSORT of: pF1KB6605.seq Check: 4945 from: 1 to: 4292
pF1KB6605 4292 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1330 1330
Size: 4292
Acc65I G'GTAC_C
Cuts at: 1308 1308
Size: 4292
AclI AA'CG_TT
Cuts at: 4132 4132
Size: 4292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 442 757 860 1088 2117 2549 3296 3564
Size: 222 315 103 228 1029 432 747 268
Cuts at: 3564 220
Size: 948
Fragments arranged by size:
1029 948 747 432 315 268 228 222
103
AflII C'TTAA_G
Cuts at: 409 409
Size: 4292
AflIII A'CryG_T
Cuts at: 1644 2748 3725 1644
Size: 1104 977 2211
Fragments arranged by size:
2211 1104 977
AgeI A'CCGG_T
Cuts at: 2680 2680
Size: 4292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 331 331
Size: 4292
AlwNI CAG_nnn'CTG
Cuts at: 871 1597 3164 871
Size: 726 1567 1999
Fragments arranged by size:
1999 1567 726
ApaI G_GGCC'C
Cuts at: 253 253
Size: 4292
ApaLI G'TGCA_C
Cuts at: 3062 3062
Size: 4292
ApoI r'AATT_y
Cuts at: 556 682 976 1296 3645 3901 556
Size: 126 294 320 2349 256 947
Fragments arranged by size:
2349 947 320 294 256 126
AseI AT'TA_AT
Cuts at: 20 20
Size: 4292
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
AvaI C'yCGr_G
Cuts at: 1312 1312
Size: 4292
BamHI G'GATC_C
Cuts at: 1317 1317
Size: 4292
BanI G'GyrC_C
Cuts at: 1202 1308 1722 1970 2005 1202
Size: 106 414 248 35 3489
Fragments arranged by size:
3489 414 248 106 35
BanII G_rGCy'C
Cuts at: 253 1306 2336 253
Size: 1053 1030 2209
Fragments arranged by size:
2209 1053 1030
BbeI G_GCGC'C
Cuts at: 1206 1726 1974 1206
Size: 520 248 3524
Fragments arranged by size:
3524 520 248
BbsI GAAGACnn'nnnn_
Cuts at: 447 492 447
Size: 45 4247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4258
BciVI GTATCCnnnnn_n'
Cuts at: 2190 2951 4292 2190
Size: 761 1341 2190
Fragments arranged by size:
2190 1341 761
BclI T'GATC_A
Cuts at: 447 1812 447
Size: 1365 2927
BglII A'GATC_T
Cuts at: 709 1807 3844 709
Size: 1098 2037 1157
Fragments arranged by size:
2037 1157 1098
BlpI GC'TnA_GC
Cuts at: 1404 1404
Size: 4292
Bme1580I G_kGCm'C
Cuts at: 119 253 1917 2010 3066 119
Size: 134 1664 93 1056 1345
Fragments arranged by size:
1664 1345 1056 134 93
BmgBI CAC'GTC
Cuts at: 637 1162 1189 637
Size: 525 27 3740
Fragments arranged by size:
3740 525 27
BmrI ACTGGGnnnn_n'
Cuts at: 786 1504 1585 1920 4064 786
Size: 718 81 335 2144 1014
Fragments arranged by size:
2144 1014 718 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 287 2659 287
Size: 2372 1920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1147 1147
Size: 4292
Bpu10I CC'TnA_GC
Cuts at: 272 272
Size: 4292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1468 2347 2839 3137 1468
Size: 879 492 298 2623
Fragments arranged by size:
2623 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1136 3996 1136
Size: 2860 1432
BsaAI yAC'GTr
Cuts at: 1645 1645
Size: 4292
BsaBI GATnn'nnATC
Cuts at: 6 1806 3843 6
Size: 1800 2037 455
Fragments arranged by size:
2037 1800 455
BsaHI Gr'CG_yC
Cuts at: 1203 1723 1971 2673 4169 1203
Size: 520 248 702 1496 1326
Fragments arranged by size:
1496 1326 702 520 248
BsaWI w'CCGG_w
Cuts at: 1094 1481 2002 2680 2954 3101 3512 1094
Size: 387 521 678 274 147 411 1874
Fragments arranged by size:
1874 678 521 411 387 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 400 665 922 400
Size: 265 257 3770
Fragments arranged by size:
3770 265 257
BseYI C'CCAG_C
Cuts at: 358 1275 1717 2475 3052 3548 358
Size: 917 442 758 577 496 1102
Fragments arranged by size:
1102 917 758 577 496 442
BsiEI CG_ry'CG
Cuts at: 104 1834 1880 3088 104
Size: 1730 46 1208 1308
Fragments arranged by size:
1730 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1306 2084 2274 3066 1306
Size: 778 190 792 2532
Fragments arranged by size:
2532 792 778 190
BsmI GAATG_Cn'
Cuts at: 1270 1270
Size: 4292
BsmBI CGTCTCn'nnnn_
Cuts at: 382 1124 382
Size: 742 3550
Bsp1286I G_dGCh'C
Cuts at: 119 253 1306 1917 2010 2084 2274 2336 3066
Size: 134 1053 611 93 74 190 62 730
Cuts at: 3066 119
Size: 1345
Fragments arranged by size:
1345 1053 730 611 190 134 93 74
62
BspEI T'CCGG_A
Cuts at: 1094 3512 1094
Size: 2418 1874
BspHI T'CATG_A
Cuts at: 3468 3468
Size: 4292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1342 1556 1858 2239 1342
Size: 214 302 381 3395
Fragments arranged by size:
3395 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2584 2638 4122 4287 50
Size: 2534 54 1484 165 55
Fragments arranged by size:
2534 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2204 2204
Size: 4292
BsrFI r'CCGG_y
Cuts at: 229 2290 2471 2680 229
Size: 2061 181 209 1841
Fragments arranged by size:
2061 1841 209 181
BssSI C'ACGA_G
Cuts at: 506 668 742 2563 2921 506
Size: 162 74 1821 358 1877
Fragments arranged by size:
1877 1821 358 162 74
BstAPI GCAn_nnn'nTGC
Cuts at: 1669 1669
Size: 4292
BstBI TT'CG_AA
Cuts at: 521 2653 521
Size: 2132 2160
BstEII G'GTnAC_C
Cuts at: 144 144
Size: 4292
BstYI r'GATC_y
Cuts at: 1 709 766 1317 1784 1807 2142 2388 3400
Size: 708 57 551 467 23 335 246 1012
Cuts at: 3400 3486 3498 3844 1
Size: 86 12 346 449
Fragments arranged by size:
1012 708 551 467 449 346 335 246
86 57 23 12
BtgI C'CryG_G
Cuts at: 278 401 630 1212 278
Size: 123 229 582 3358
Fragments arranged by size:
3358 582 229 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2242 2423 2586 2242
Size: 181 163 3948
Fragments arranged by size:
3948 181 163
BtsI GCAGTG_nn'
Cuts at: 1021 1535 1021
Size: 514 3778
DraI TTT'AAA
Cuts at: 1291 1291
Size: 4292
DraIII CAC_nnn'GTG
Cuts at: 508 508
Size: 4292
DrdI GACnn_nn'nnGTC
Cuts at: 1230 1998 2856 1230
Size: 768 858 2666
Fragments arranged by size:
2666 858 768
EaeI y'GGCC_r
Cuts at: 1149 1877 2051 2442 2469 3814 1149
Size: 728 174 391 27 1345 1627
Fragments arranged by size:
1627 1345 728 391 174 27
EagI C'GGCC_G
Cuts at: 1877 1877
Size: 4292
EarI CTCTTCn'nnn_
Cuts at: 609 890 1049 2315 2525 609
Size: 281 159 1266 210 2376
Fragments arranged by size:
2376 1266 281 210 159
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2500 2810 2956 4102 2500
Size: 310 146 1146 2690
Fragments arranged by size:
2690 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1304 1304
Size: 4292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 442 757 860 1088 1147 2117 2549 3296
Size: 222 315 103 228 59 970 432 747
Cuts at: 3296 3564 220
Size: 268 948
Fragments arranged by size:
970 948 747 432 315 268 228 222
103 59
EcoNI CCTnn'n_nnAGG
Cuts at: 593 593
Size: 4292
EcoO109I rG'GnC_Cy
Cuts at: 249 250 1431 249
Size: 1 1181 3110
Fragments arranged by size:
3110 1181 1
EcoRI G'AATT_C
Cuts at: 1296 1296
Size: 4292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2311 2311
Size: 4292
FspI TGC'GCA
Cuts at: 319 2073 319
Size: 1754 2538
HaeII r_GCGC'y
Cuts at: 409 1206 1726 1974 2996 3975 409
Size: 797 520 248 1022 979 726
Fragments arranged by size:
1022 979 797 726 520 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 1102 1134 2134 2164 2166 2196 163
Size: 32 907 32 1000 30 2 30 2259
Fragments arranged by size:
2259 1000 907 32 32 30 30 2
HincII GTy'rAC
Cuts at: 375 1331 375
Size: 956 3336
KasI G'GCGC_C
Cuts at: 1202 1722 1970 1202
Size: 520 248 3524
Fragments arranged by size:
3524 520 248
KpnI G_GTAC'C
Cuts at: 1312 1312
Size: 4292
MluI A'CGCG_T
Cuts at: 3725 3725
Size: 4292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 320 414 2963 3147 320
Size: 94 2549 184 1465
Fragments arranged by size:
2549 1465 184 94
MscI TGG'CCA
Cuts at: 2053 2053
Size: 4292
MslI CAynn'nnrTG
Cuts at: 728 2281 2408 728
Size: 1553 127 2612
Fragments arranged by size:
2612 1553 127
MspA1I CmG'CkG
Cuts at: 280 315 351 1125 1403 1717 2077 3090 3335
Size: 35 36 774 278 314 360 1013 245
Cuts at: 3335 280
Size: 1237
Fragments arranged by size:
1237 1013 774 360 314 278 245 36
35
NaeI GCC'GGC
Cuts at: 231 2473 231
Size: 2242 2050
NarI GG'CG_CC
Cuts at: 1203 1723 1971 1203
Size: 520 248 3524
Fragments arranged by size:
3524 520 248
NgoMIV G'CCGG_C
Cuts at: 229 2471 229
Size: 2242 2050
NspI r_CATG'y
Cuts at: 1345 1845 2752 1345
Size: 500 907 2885
Fragments arranged by size:
2885 907 500
PasI CC'CwG_GG
Cuts at: 253 253
Size: 4292
PciI A'CATG_T
Cuts at: 2748 2748
Size: 4292
PflMI CCAn_nnn'nTGG
Cuts at: 1162 1213 1162
Size: 51 4241
PmeI GTTT'AAAC
Cuts at: 1291 1291
Size: 4292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 4260
PspOMI G'GGCC_C
Cuts at: 249 249
Size: 4292
PstI C_TGCA'G
Cuts at: 1339 2024 1339
Size: 685 3607
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4292
PvuII CAG'CTG
Cuts at: 315 1125 1717 2077 315
Size: 810 592 360 2530
Fragments arranged by size:
2530 810 592 360
RsrII CG'GwC_CG
Cuts at: 2487 2487
Size: 4292
SacI G_AGCT'C
Cuts at: 1306 1306
Size: 4292
SacII CC_GC'GG
Cuts at: 281 281
Size: 4292
SalI G'TCGA_C
Cuts at: 1329 1329
Size: 4292
SapI GCTCTTCn'nnn_
Cuts at: 2315 2525 2315
Size: 210 4082
SbfI CC_TGCA'GG
Cuts at: 1339 1339
Size: 4292
ScaI AGT'ACT
Cuts at: 3809 3809
Size: 4292
SfcI C'TryA_G
Cuts at: 33 1335 2020 3013 3204 33
Size: 1302 685 993 191 1121
Fragments arranged by size:
1302 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1204 1724 1972 1204
Size: 520 248 3524
Fragments arranged by size:
3524 520 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4292
SmaI CCC'GGG
Cuts at: 1314 1314
Size: 4292
SmlI C'TyrA_G
Cuts at: 409 1447 2362 2854 3116 409
Size: 1038 915 492 262 1585
Fragments arranged by size:
1585 1038 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4292
SphI G_CATG'C
Cuts at: 1345 1845 1345
Size: 500 3792
StyI C'CwwG_G
Cuts at: 1146 1426 1780 1146
Size: 280 354 3658
Fragments arranged by size:
3658 354 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 588 599 1240 1875 2008 2467 2676 588
Size: 11 641 635 133 459 209 2204
Fragments arranged by size:
2204 641 635 459 209 133 11
TatI w'GTAC_w
Cuts at: 3807 3807
Size: 4292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4260
Tth111I GACn'n_nGTC
Cuts at: 2089 2089
Size: 4292
XbaI T'CTAG_A
Cuts at: 1323 1323
Size: 4292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 366 1787 366
Size: 1421 2871
XmaI C'CCGG_G
Cuts at: 1312 1312
Size: 4292
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsaXI BsgI BsiWI
BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI