Restriction map for
pF1KB6607
(Circular) (Six-base) MAPSORT of: pF1KB6607.seq Check: 4471 from: 1 to: 4340
pF1KB6607 4340 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 483 531 483
Size: 48 4292
AccI GT'mk_AC
Cuts at: 1378 1378
Size: 4340
Acc65I G'GTAC_C
Cuts at: 1356 1356
Size: 4340
AclI AA'CG_TT
Cuts at: 4180 4180
Size: 4340
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 848 1033 1265 2165 2597 3344 3612 142
Size: 706 185 232 900 432 747 268 870
Fragments arranged by size:
900 870 747 706 432 268 232 185
AflIII A'CryG_T
Cuts at: 1692 2796 3773 1692
Size: 1104 977 2259
Fragments arranged by size:
2259 1104 977
AgeI A'CCGG_T
Cuts at: 2728 2728
Size: 4340
AleI CACnn'nnGTG
Cuts at: 424 424
Size: 4340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 746 746
Size: 4340
AlwNI CAG_nnn'CTG
Cuts at: 385 598 874 1162 1645 3212 385
Size: 213 276 288 483 1567 1513
Fragments arranged by size:
1567 1513 483 288 276 213
ApaLI G'TGCA_C
Cuts at: 653 3110 653
Size: 2457 1883
ApoI r'AATT_y
Cuts at: 937 1290 1344 3693 3949 937
Size: 353 54 2349 256 1328
Fragments arranged by size:
2349 1328 353 256 54
AseI AT'TA_AT
Cuts at: 20 244 20
Size: 224 4116
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
AvaI C'yCGr_G
Cuts at: 163 1360 163
Size: 1197 3143
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1249 1282 1249
Size: 33 4307
BamHI G'GATC_C
Cuts at: 1365 1365
Size: 4340
BanI G'GyrC_C
Cuts at: 1356 1770 2018 2053 1356
Size: 414 248 35 3643
Fragments arranged by size:
3643 414 248 35
BanII G_rGCy'C
Cuts at: 855 1354 2384 855
Size: 499 1030 2811
Fragments arranged by size:
2811 1030 499
BbeI G_GCGC'C
Cuts at: 1774 2022 1774
Size: 248 4092
BbsI GAAGACnn'nnnn_
Cuts at: 130 854 130
Size: 724 3616
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1009 1043 90
Size: 34 885 34 3387
Fragments arranged by size:
3387 885 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2238 2999 4340 2238
Size: 761 1341 2238
Fragments arranged by size:
2238 1341 761
BclI T'GATC_A
Cuts at: 1860 1860
Size: 4340
BglII A'GATC_T
Cuts at: 1855 3892 1855
Size: 2037 2303
BlpI GC'TnA_GC
Cuts at: 1452 1452
Size: 4340
Bme1580I G_kGCm'C
Cuts at: 657 1965 2058 3114 657
Size: 1308 93 1056 1883
Fragments arranged by size:
1883 1308 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 857 1552 1633 1968 4112 857
Size: 695 81 335 2144 1085
Fragments arranged by size:
2144 1085 695 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2707 2707
Size: 4340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 473 818 941 115
Size: 358 345 123 3514
Fragments arranged by size:
3514 358 345 123
Bpu10I CC'TnA_GC
Cuts at: 431 431
Size: 4340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1516 2395 2887 3185 818
Size: 698 879 492 298 1973
Fragments arranged by size:
1973 879 698 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4044 4044
Size: 4340
BsaAI yAC'GTr
Cuts at: 1693 1693
Size: 4340
BsaBI GATnn'nnATC
Cuts at: 6 1854 3891 6
Size: 1848 2037 455
Fragments arranged by size:
2037 1848 455
BsaHI Gr'CG_yC
Cuts at: 1771 2019 2721 4217 1771
Size: 248 702 1496 1894
Fragments arranged by size:
1894 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1529 2050 2728 3002 3149 3560 1529
Size: 521 678 274 147 411 2309
Fragments arranged by size:
2309 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 837 867 837
Size: 30 4310
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 845 1139 1142 181
Size: 664 294 3 3379
Fragments arranged by size:
3379 664 294 3
BseYI C'CCAG_C
Cuts at: 1765 2523 3100 3596 1765
Size: 758 577 496 2509
Fragments arranged by size:
2509 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 503 551 503
Size: 48 4292
BsiEI CG_ry'CG
Cuts at: 104 1882 1928 3136 104
Size: 1778 46 1208 1308
Fragments arranged by size:
1778 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 657 1354 2132 2322 3114 657
Size: 697 778 190 792 1883
Fragments arranged by size:
1883 792 778 697 190
BsmBI CGTCTCn'nnnn_
Cuts at: 163 163
Size: 4340
Bsp1286I G_dGCh'C
Cuts at: 657 855 1354 1965 2058 2132 2322 2384 3114
Size: 198 499 611 93 74 190 62 730
Cuts at: 3114 657
Size: 1883
Fragments arranged by size:
1883 730 611 499 198 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3560 3560
Size: 4340
BspHI T'CATG_A
Cuts at: 3516 3516
Size: 4340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 102 223 483 525 531 1390 1604 1906 2287
Size: 121 260 42 6 859 214 302 381
Cuts at: 2287 102
Size: 2155
Fragments arranged by size:
2155 859 381 302 260 214 121 42
6
BsrBI CCG'CTC
Cuts at: 50 2632 2686 4170 4335 50
Size: 2582 54 1484 165 55
Fragments arranged by size:
2582 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2252 2252
Size: 4340
BsrFI r'CCGG_y
Cuts at: 2338 2519 2728 2338
Size: 181 209 3950
Fragments arranged by size:
3950 209 181
BssSI C'ACGA_G
Cuts at: 2611 2969 2611
Size: 358 3982
BstAPI GCAn_nnn'nTGC
Cuts at: 652 874 1717 652
Size: 222 843 3275
Fragments arranged by size:
3275 843 222
BstBI TT'CG_AA
Cuts at: 2701 2701
Size: 4340
BstEII G'GTnAC_C
Cuts at: 114 327 114
Size: 213 4127
BstXI CCAn_nnnn'nTGG
Cuts at: 426 426
Size: 4340
BstYI r'GATC_y
Cuts at: 1 1365 1832 1855 2190 2436 3448 3534 3546
Size: 1364 467 23 335 246 1012 86 12
Cuts at: 3546 3892 1
Size: 346 449
Fragments arranged by size:
1364 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1199 1199
Size: 4340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2290 2471 2634 2290
Size: 181 163 3996
Fragments arranged by size:
3996 181 163
BtsI GCAGTG_nn'
Cuts at: 350 776 875 1149 1583 350
Size: 426 99 274 434 3107
Fragments arranged by size:
3107 434 426 274 99
DraI TTT'AAA
Cuts at: 1339 1339
Size: 4340
DrdI GACnn_nn'nnGTC
Cuts at: 669 2046 2904 669
Size: 1377 858 2105
Fragments arranged by size:
2105 1377 858
EaeI y'GGCC_r
Cuts at: 1925 2099 2490 2517 3862 1925
Size: 174 391 27 1345 2403
Fragments arranged by size:
2403 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1925 1925
Size: 4340
EarI CTCTTCn'nnn_
Cuts at: 2363 2573 2363
Size: 210 4130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2548 2858 3004 4150 2548
Size: 310 146 1146 2738
Fragments arranged by size:
2738 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1352 1352
Size: 4340
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 142 473 818 848 941 1033 1265 2165
Size: 27 331 345 30 93 92 232 900
Cuts at: 2165 2597 3344 3612 115
Size: 432 747 268 843
Fragments arranged by size:
900 843 747 432 345 331 268 232
93 92 30 27
EcoO109I rG'GnC_Cy
Cuts at: 1479 1479
Size: 4340
EcoRI G'AATT_C
Cuts at: 1344 1344
Size: 4340
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2359 2359
Size: 4340
FspI TGC'GCA
Cuts at: 2121 2121
Size: 4340
HaeII r_GCGC'y
Cuts at: 1774 2022 3044 4023 1774
Size: 248 1022 979 2091
Fragments arranged by size:
2091 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 592 624 685 717 2182 2212 2214 2244 592
Size: 32 61 32 1465 30 2 30 2688
Fragments arranged by size:
2688 1465 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 825 1379 825
Size: 554 3786
KasI G'GCGC_C
Cuts at: 1770 2018 1770
Size: 248 4092
KpnI G_GTAC'C
Cuts at: 1360 1360
Size: 4340
MluI A'CGCG_T
Cuts at: 3773 3773
Size: 4340
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 463 3011 3195 463
Size: 2548 184 1608
Fragments arranged by size:
2548 1608 184
MscI TGG'CCA
Cuts at: 2101 2101
Size: 4340
MslI CAynn'nnrTG
Cuts at: 424 703 2329 2456 424
Size: 279 1626 127 2308
Fragments arranged by size:
2308 1626 279 127
MspA1I CmG'CkG
Cuts at: 880 1451 1765 2125 3138 3383 880
Size: 571 314 360 1013 245 1837
Fragments arranged by size:
1837 1013 571 360 314 245
NaeI GCC'GGC
Cuts at: 2521 2521
Size: 4340
NarI GG'CG_CC
Cuts at: 1771 2019 1771
Size: 248 4092
NdeI CA'TA_TG
Cuts at: 1175 1175
Size: 4340
NgoMIV G'CCGG_C
Cuts at: 2519 2519
Size: 4340
NspI r_CATG'y
Cuts at: 1393 1893 2800 1393
Size: 500 907 2933
Fragments arranged by size:
2933 907 500
PciI A'CATG_T
Cuts at: 2796 2796
Size: 4340
PmeI GTTT'AAAC
Cuts at: 1339 1339
Size: 4340
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 3479
Size: 32 4308
PstI C_TGCA'G
Cuts at: 555 879 885 1387 2072 555
Size: 324 6 502 685 2823
Fragments arranged by size:
2823 685 502 324 6
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4340
PvuII CAG'CTG
Cuts at: 880 1765 2125 880
Size: 885 360 3095
Fragments arranged by size:
3095 885 360
RsrII CG'GwC_CG
Cuts at: 2535 2535
Size: 4340
SacI G_AGCT'C
Cuts at: 1354 1354
Size: 4340
SalI G'TCGA_C
Cuts at: 1377 1377
Size: 4340
SapI GCTCTTCn'nnn_
Cuts at: 2363 2573 2363
Size: 210 4130
SbfI CC_TGCA'GG
Cuts at: 1387 1387
Size: 4340
ScaI AGT'ACT
Cuts at: 846 3857 846
Size: 3011 1329
SfcI C'TryA_G
Cuts at: 33 395 551 875 881 1015 1214 1312 1383
Size: 362 156 324 6 134 199 98 71
Cuts at: 1383 2068 3061 3252 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 362 324 199 191 156
134 98 71 6
SfoI GGC'GCC
Cuts at: 1772 2020 1772
Size: 248 4092
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4340
SmaI CCC'GGG
Cuts at: 1362 1362
Size: 4340
SmlI C'TyrA_G
Cuts at: 833 1495 2410 2902 3164 833
Size: 662 915 492 262 2009
Fragments arranged by size:
2009 915 662 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4340
SphI G_CATG'C
Cuts at: 1393 1893 1393
Size: 500 3840
StyI C'CwwG_G
Cuts at: 455 1474 1828 455
Size: 1019 354 2967
Fragments arranged by size:
2967 1019 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 614 1923 2056 2515 2724 614
Size: 1309 133 459 209 2230
Fragments arranged by size:
2230 1309 459 209 133
TatI w'GTAC_w
Cuts at: 604 844 3855 604
Size: 240 3011 1089
Fragments arranged by size:
3011 1089 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3857 3889 3857
Size: 32 4308
Tth111I GACn'n_nGTC
Cuts at: 2137 2137
Size: 4340
XbaI T'CTAG_A
Cuts at: 1371 1371
Size: 4340
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 1835 423
Size: 1412 2928
XmaI C'CCGG_G
Cuts at: 1360 1360
Size: 4340
XmnI GAAnn'nnTTC
Cuts at: 1100 1100
Size: 4340
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstZ17I BtgI ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI