Restriction map for
pF1KB6610
(Circular) (Six-base) MAPSORT of: pF1KB6610.seq Check: 4187 from: 1 to: 4403
pF1KB6610 4403 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1441 1441
Size: 4403
Acc65I G'GTAC_C
Cuts at: 1419 1419
Size: 4403
AclI AA'CG_TT
Cuts at: 4243 4243
Size: 4403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 451 497 2228 2660 3407 3675 451
Size: 46 1731 432 747 268 1179
Fragments arranged by size:
1731 1179 747 432 268 46
AfeI AGC'GCT
Cuts at: 140 775 140
Size: 635 3768
AflIII A'CryG_T
Cuts at: 1755 2859 3836 1755
Size: 1104 977 2322
Fragments arranged by size:
2322 1104 977
AgeI A'CCGG_T
Cuts at: 2791 2791
Size: 4403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 881 1184 881
Size: 303 4100
AlwNI CAG_nnn'CTG
Cuts at: 644 1015 1708 3275 644
Size: 371 693 1567 1772
Fragments arranged by size:
1772 1567 693 371
ApaI G_GGCC'C
Cuts at: 441 441
Size: 4403
ApaLI G'TGCA_C
Cuts at: 871 3173 871
Size: 2302 2101
ApoI r'AATT_y
Cuts at: 340 1407 3756 4012 340
Size: 1067 2349 256 731
Fragments arranged by size:
2349 1067 731 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4403
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
AvaI C'yCGr_G
Cuts at: 876 1288 1423 876
Size: 412 135 3856
Fragments arranged by size:
3856 412 135
BamHI G'GATC_C
Cuts at: 1428 1428
Size: 4403
BanI G'GyrC_C
Cuts at: 517 1059 1168 1419 1833 2081 2116 517
Size: 542 109 251 414 248 35 2804
Fragments arranged by size:
2804 542 414 251 248 109 35
BanII G_rGCy'C
Cuts at: 195 278 441 586 958 1214 1229 1391 1417
Size: 83 163 145 372 256 15 162 26
Cuts at: 1417 2447 195
Size: 1030 2151
Fragments arranged by size:
2151 1030 372 256 163 162 145 83
26 15
BbeI G_GCGC'C
Cuts at: 1063 1837 2085 1063
Size: 774 248 3381
Fragments arranged by size:
3381 774 248
BbsI GAAGACnn'nnnn_
Cuts at: 604 604
Size: 4403
BbvCI CC'TCA_GC
Cuts at: 935 949 1049 935
Size: 14 100 4289
Fragments arranged by size:
4289 100 14
BciVI GTATCCnnnnn_n'
Cuts at: 2301 3062 4403 2301
Size: 761 1341 2301
Fragments arranged by size:
2301 1341 761
BclI T'GATC_A
Cuts at: 1923 1923
Size: 4403
BglI GCCn_nnn'nGGC
Cuts at: 112 706 1361 112
Size: 594 655 3154
Fragments arranged by size:
3154 655 594
BglII A'GATC_T
Cuts at: 1038 1274 1918 3955 1038
Size: 236 644 2037 1486
Fragments arranged by size:
2037 1486 644 236
BlpI GC'TnA_GC
Cuts at: 1515 1515
Size: 4403
Bme1580I G_kGCm'C
Cuts at: 441 875 1236 1242 2028 2121 3177 441
Size: 434 361 6 786 93 1056 1667
Fragments arranged by size:
1667 1056 786 434 361 93 6
BmgBI CAC'GTC
Cuts at: 408 561 815 408
Size: 153 254 3996
Fragments arranged by size:
3996 254 153
BmrI ACTGGGnnnn_n'
Cuts at: 198 571 1615 1696 2031 4175 198
Size: 373 1044 81 335 2144 426
Fragments arranged by size:
2144 1044 426 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2770 2770
Size: 4403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 703 742 912 1103 277
Size: 426 39 170 191 3577
Fragments arranged by size:
3577 426 191 170 39
Bpu10I CC'TnA_GC
Cuts at: 121 935 949 1049 121
Size: 814 14 100 3475
Fragments arranged by size:
3475 814 100 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1008 1579 2458 2950 3248 1008
Size: 571 879 492 298 2163
Fragments arranged by size:
2163 879 571 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 982 1123 4107 982
Size: 141 2984 1278
Fragments arranged by size:
2984 1278 141
BsaAI yAC'GTr
Cuts at: 156 1383 1756 156
Size: 1227 373 2803
Fragments arranged by size:
2803 1227 373
BsaBI GATnn'nnATC
Cuts at: 6 1917 3954 6
Size: 1911 2037 455
Fragments arranged by size:
2037 1911 455
BsaHI Gr'CG_yC
Cuts at: 1060 1834 2082 2784 4280 1060
Size: 774 248 702 1496 1183
Fragments arranged by size:
1496 1183 774 702 248
BsaWI w'CCGG_w
Cuts at: 1592 2113 2791 3065 3212 3623 1592
Size: 521 678 274 147 411 2372
Fragments arranged by size:
2372 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 469 499 1090 1120 469
Size: 30 591 30 3752
Fragments arranged by size:
3752 591 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 274 286 481 679 682 718 966 1061 1066
Size: 12 195 198 3 36 248 95 5
Cuts at: 1066 1111 274
Size: 45 3566
Fragments arranged by size:
3566 248 198 195 95 45 36 12
5 3
BseYI C'CCAG_C
Cuts at: 1063 1828 2586 3163 3659 1063
Size: 765 758 577 496 1807
Fragments arranged by size:
1807 765 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 743 1105 1177 743
Size: 362 72 3969
Fragments arranged by size:
3969 362 72
BsiEI CG_ry'CG
Cuts at: 104 185 1945 1991 3199 104
Size: 81 1760 46 1208 1308
Fragments arranged by size:
1760 1308 1208 81 46
BsiHKAI G_wGCw'C
Cuts at: 278 875 1184 1391 1417 2195 2385 3177 278
Size: 597 309 207 26 778 190 792 1504
Fragments arranged by size:
1504 792 778 597 309 207 190 26
Bsp1286I G_dGCh'C
Cuts at: 195 278 441 586 875 958 1184 1214 1229
Size: 83 163 145 289 83 226 30 15
Cuts at: 1229 1236 1242 1391 1417 2028 2121 2195 2385
Size: 7 6 149 26 611 93 74 190
Cuts at: 2385 2447 3177 195
Size: 62 730 1421
Fragments arranged by size:
1421 730 611 289 226 190 163 149
145 93 83 83 74 62 30 26
15 7 6
BspEI T'CCGG_A
Cuts at: 3623 3623
Size: 4403
BspHI T'CATG_A
Cuts at: 3579 3579
Size: 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1453 1667 1969 2350 1453
Size: 214 302 381 3506
Fragments arranged by size:
3506 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2695 2749 4233 4398 50
Size: 2645 54 1484 165 55
Fragments arranged by size:
2645 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 225 2315 225
Size: 2090 2313
BsrFI r'CCGG_y
Cuts at: 1056 1171 2401 2582 2791 1056
Size: 115 1230 181 209 2668
Fragments arranged by size:
2668 1230 209 181 115
BssSI C'ACGA_G
Cuts at: 2674 3032 2674
Size: 358 4045
BstAPI GCAn_nnn'nTGC
Cuts at: 1780 1780
Size: 4403
BstBI TT'CG_AA
Cuts at: 2764 2764
Size: 4403
BstEII G'GTnAC_C
Cuts at: 165 165
Size: 4403
BstXI CCAn_nnnn'nTGG
Cuts at: 1383 1383
Size: 4403
BstYI r'GATC_y
Cuts at: 1 1038 1274 1428 1895 1918 2253 2499 3511
Size: 1037 236 154 467 23 335 246 1012
Cuts at: 3511 3597 3609 3955 1
Size: 86 12 346 449
Fragments arranged by size:
1037 1012 467 449 346 335 246 236
154 86 23 12
BtgI C'CryG_G
Cuts at: 107 416 107
Size: 309 4094
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2353 2534 2697 2353
Size: 181 163 4059
Fragments arranged by size:
4059 181 163
BtsI GCAGTG_nn'
Cuts at: 1646 1646
Size: 4403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 525 560 525
Size: 35 4368
DraI TTT'AAA
Cuts at: 1402 1402
Size: 4403
DrdI GACnn_nn'nnGTC
Cuts at: 2109 2967 2109
Size: 858 3545
EaeI y'GGCC_r
Cuts at: 419 503 546 1353 1988 2162 2553 2580 3925
Size: 84 43 807 635 174 391 27 1345
Cuts at: 3925 419
Size: 897
Fragments arranged by size:
1345 897 807 635 391 174 84 43
27
EagI C'GGCC_G
Cuts at: 1988 1988
Size: 4403
EarI CTCTTCn'nnn_
Cuts at: 1088 2426 2636 1088
Size: 1338 210 2855
Fragments arranged by size:
2855 1338 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2611 2921 3067 4213 2611
Size: 310 146 1146 2801
Fragments arranged by size:
2801 1146 310 146
EcoICRI GAG'CTC
Cuts at: 276 1389 1415 276
Size: 1113 26 3264
Fragments arranged by size:
3264 1113 26
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 451 497 703 742 912 1103 2228 2660
Size: 174 46 206 39 170 191 1125 432
Cuts at: 2660 3407 3675 277
Size: 747 268 1005
Fragments arranged by size:
1125 1005 747 432 268 206 191 174
170 46 39
EcoO109I rG'GnC_Cy
Cuts at: 318 437 565 573 1542 318
Size: 119 128 8 969 3179
Fragments arranged by size:
3179 969 128 119 8
EcoRI G'AATT_C
Cuts at: 1407 1407
Size: 4403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 4403
FspI TGC'GCA
Cuts at: 2184 2184
Size: 4403
HaeII r_GCGC'y
Cuts at: 142 735 777 1063 1837 2085 3107 4086 142
Size: 593 42 286 774 248 1022 979 459
Fragments arranged by size:
1022 979 774 593 459 286 248 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 310 342 847 879 2245 2275 2277 2307 310
Size: 32 505 32 1366 30 2 30 2406
Fragments arranged by size:
2406 1366 505 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1442 1442
Size: 4403
KasI G'GCGC_C
Cuts at: 1059 1833 2081 1059
Size: 774 248 3381
Fragments arranged by size:
3381 774 248
KpnI G_GTAC'C
Cuts at: 1423 1423
Size: 4403
MluI A'CGCG_T
Cuts at: 3836 3836
Size: 4403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3074 3258 3074
Size: 184 4219
MscI TGG'CCA
Cuts at: 421 505 1355 2164 421
Size: 84 850 809 2660
Fragments arranged by size:
2660 850 809 84
MslI CAynn'nnrTG
Cuts at: 229 1312 2392 2519 229
Size: 1083 1080 127 2113
Fragments arranged by size:
2113 1083 1080 127
MspA1I CmG'CkG
Cuts at: 284 714 720 1012 1514 1828 2188 3201 3446
Size: 430 6 292 502 314 360 1013 245
Cuts at: 3446 284
Size: 1241
Fragments arranged by size:
1241 1013 502 430 360 314 292 245
6
NaeI GCC'GGC
Cuts at: 1058 1173 2584 1058
Size: 115 1411 2877
Fragments arranged by size:
2877 1411 115
NarI GG'CG_CC
Cuts at: 1060 1834 2082 1060
Size: 774 248 3381
Fragments arranged by size:
3381 774 248
NcoI C'CATG_G
Cuts at: 107 416 107
Size: 309 4094
NgoMIV G'CCGG_C
Cuts at: 1056 1171 2582 1056
Size: 115 1411 2877
Fragments arranged by size:
2877 1411 115
NspI r_CATG'y
Cuts at: 1368 1456 1956 2863 1368
Size: 88 500 907 2908
Fragments arranged by size:
2908 907 500 88
PasI CC'CwG_GG
Cuts at: 1031 1031
Size: 4403
PciI A'CATG_T
Cuts at: 2859 2859
Size: 4403
PflMI CCAn_nnn'nTGG
Cuts at: 561 561
Size: 4403
PfoI T'CCnGG_A
Cuts at: 554 554
Size: 4403
PmeI GTTT'AAAC
Cuts at: 1402 1402
Size: 4403
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3542 3574 3542
Size: 32 4371
PpuMI rG'GwC_Cy
Cuts at: 318 565 318
Size: 247 4156
PspOMI G'GGCC_C
Cuts at: 437 437
Size: 4403
PstI C_TGCA'G
Cuts at: 310 968 1450 2135 310
Size: 658 482 685 2578
Fragments arranged by size:
2578 685 658 482
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4403
PvuII CAG'CTG
Cuts at: 720 1012 1828 2188 720
Size: 292 816 360 2935
Fragments arranged by size:
2935 816 360 292
RsrII CG'GwC_CG
Cuts at: 2598 2598
Size: 4403
SacI G_AGCT'C
Cuts at: 278 1391 1417 278
Size: 1113 26 3264
Fragments arranged by size:
3264 1113 26
SalI G'TCGA_C
Cuts at: 1440 1440
Size: 4403
SanDI GG'GwC_CC
Cuts at: 565 565
Size: 4403
SapI GCTCTTCn'nnn_
Cuts at: 2426 2636 2426
Size: 210 4193
SbfI CC_TGCA'GG
Cuts at: 1450 1450
Size: 4403
ScaI AGT'ACT
Cuts at: 3920 3920
Size: 4403
SfcI C'TryA_G
Cuts at: 33 306 525 964 1446 2131 3124 3315 33
Size: 273 219 439 482 685 993 191 1121
Fragments arranged by size:
1121 993 685 482 439 273 219 191
SfoI GGC'GCC
Cuts at: 1061 1835 2083 1061
Size: 774 248 3381
Fragments arranged by size:
3381 774 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4403
SmaI CCC'GGG
Cuts at: 1425 1425
Size: 4403
SmlI C'TyrA_G
Cuts at: 1023 1558 2473 2965 3227 1023
Size: 535 915 492 262 2199
Fragments arranged by size:
2199 915 535 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4403
SphI G_CATG'C
Cuts at: 1456 1956 1456
Size: 500 3903
StuI AGG'CCT
Cuts at: 151 151
Size: 4403
StyI C'CwwG_G
Cuts at: 107 416 506 1537 1891 107
Size: 309 90 1031 354 2619
Fragments arranged by size:
2619 1031 354 309 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 199 994 1986 2119 2578 2787 199
Size: 795 992 133 459 209 1815
Fragments arranged by size:
1815 992 795 459 209 133
TatI w'GTAC_w
Cuts at: 3918 3918
Size: 4403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 469 501 3920 3952 469
Size: 32 3419 32 920
Fragments arranged by size:
3419 920 32 32
Tth111I GACn'n_nGTC
Cuts at: 2200 2200
Size: 4403
XbaI T'CTAG_A
Cuts at: 1434 1434
Size: 4403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1213 1898 1213
Size: 685 3718
XmaI C'CCGG_G
Cuts at: 1423 1423
Size: 4403
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstZ17I Bsu36I ClaI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
XmnI ZraI