Restriction map for
pF1KB6611
(Circular) (Six-base) MAPSORT of: pF1KB6611.seq Check: 4951 from: 1 to: 4349
pF1KB6611 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1089 1089
Size: 4349
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AflIII A'CryG_T
Cuts at: 1025 1701 2805 3782 1025
Size: 676 1104 977 1592
Fragments arranged by size:
1592 1104 977 676
AgeI A'CCGG_T
Cuts at: 413 2737 413
Size: 2324 2025
AleI CACnn'nnGTG
Cuts at: 1082 1082
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 731 731
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 171 1654 3221 171
Size: 1483 1567 1299
Fragments arranged by size:
1567 1483 1299
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 117 1353 3702 3958 117
Size: 1236 2349 256 508
Fragments arranged by size:
2349 1236 508 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 693 1369 693
Size: 676 3673
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 407 431 672 988 1365 1779 2027 2062 407
Size: 24 241 316 377 414 248 35 2694
Fragments arranged by size:
2694 414 377 316 248 241 35 24
BanII G_rGCy'C
Cuts at: 512 1190 1271 1363 2393 512
Size: 678 81 92 1030 2468
Fragments arranged by size:
2468 1030 678 92 81
BbeI G_GCGC'C
Cuts at: 992 1783 2031 992
Size: 791 248 3310
Fragments arranged by size:
3310 791 248
BbsI GAAGACnn'nnnn_
Cuts at: 140 1104 140
Size: 964 3385
BciVI GTATCCnnnnn_n'
Cuts at: 370 838 2247 3008 4349 370
Size: 468 1409 761 1341 370
Fragments arranged by size:
1409 1341 761 468 370
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 791 791
Size: 4349
BglII A'GATC_T
Cuts at: 971 1864 3901 971
Size: 893 2037 1419
Fragments arranged by size:
2037 1419 893
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 675 1974 2067 3123 675
Size: 1299 93 1056 1901
Fragments arranged by size:
1901 1299 1056 93
BmgBI CAC'GTC
Cuts at: 1026 1026
Size: 4349
BmrI ACTGGGnnnn_n'
Cuts at: 1561 1642 1977 4121 1561
Size: 81 335 2144 1789
Fragments arranged by size:
2144 1789 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 792 2716 792
Size: 1924 2425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 910 701
Size: 209 4140
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 331 349 1525 2404 2896 3194 331
Size: 18 1176 879 492 298 1486
Fragments arranged by size:
1486 1176 879 492 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 927 1017 1702 927
Size: 90 685 3574
Fragments arranged by size:
3574 685 90
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 700 878 989 1780 2028 2730 4226 700
Size: 178 111 791 248 702 1496 823
Fragments arranged by size:
1496 823 791 702 248 178 111
BsaWI w'CCGG_w
Cuts at: 413 1538 2059 2737 3011 3158 3569 413
Size: 1125 521 678 274 147 411 1193
Fragments arranged by size:
1193 1125 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1012 1042 1012
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 964 1198 964
Size: 234 4115
BseYI C'CCAG_C
Cuts at: 497 872 1264 1774 2532 3109 3605 497
Size: 375 392 510 758 577 496 1241
Fragments arranged by size:
1241 758 577 510 496 392 375
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 691 484
Size: 207 4142
BsiEI CG_ry'CG
Cuts at: 104 981 1891 1937 3145 104
Size: 877 910 46 1208 1308
Fragments arranged by size:
1308 1208 910 877 46
BsiHKAI G_wGCw'C
Cuts at: 325 512 1190 1237 1363 2141 2331 3123 325
Size: 187 678 47 126 778 190 792 1551
Fragments arranged by size:
1551 792 778 678 190 187 126 47
BsmI GAATG_Cn'
Cuts at: 1309 1309
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 325 512 675 1190 1237 1271 1363 1974 2067
Size: 187 163 515 47 34 92 611 93
Cuts at: 2067 2141 2331 2393 3123 325
Size: 74 190 62 730 1551
Fragments arranged by size:
1551 730 611 515 190 187 163 93
92 74 62 47 34
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1089 1103 1399 1613 1915 2296 1089
Size: 14 296 214 302 381 3142
Fragments arranged by size:
3142 381 302 296 214 14
BsrBI CCG'CTC
Cuts at: 50 960 1134 2641 2695 4179 4344 50
Size: 910 174 1507 54 1484 165 55
Fragments arranged by size:
1507 1484 910 174 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 413 2347 2528 2737 413
Size: 1934 181 209 2025
Fragments arranged by size:
2025 1934 209 181
BsrGI T'GTAC_A
Cuts at: 590 1028 1103 590
Size: 438 75 3836
Fragments arranged by size:
3836 438 75
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 1726 1726
Size: 4349
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 459 459
Size: 4349
BstYI r'GATC_y
Cuts at: 1 266 971 1374 1841 1864 2199 2445 3457
Size: 265 705 403 467 23 335 246 1012
Cuts at: 3457 3543 3555 3901 1
Size: 86 12 346 449
Fragments arranged by size:
1012 705 467 449 403 346 335 265
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 334 577 1034 1220 107
Size: 227 243 457 186 3236
Fragments arranged by size:
3236 457 243 227 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1131 2299 2480 2643 1131
Size: 1168 181 163 2837
Fragments arranged by size:
2837 1168 181 163
BtsI GCAGTG_nn'
Cuts at: 703 1592 703
Size: 889 3460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 794 829 794
Size: 35 4314
DraI TTT'AAA
Cuts at: 122 1348 122
Size: 1226 3123
DrdI GACnn_nn'nnGTC
Cuts at: 238 2055 2913 238
Size: 1817 858 1674
Fragments arranged by size:
1817 1674 858
EaeI y'GGCC_r
Cuts at: 182 449 1934 2108 2499 2526 3871 182
Size: 267 1485 174 391 27 1345 660
Fragments arranged by size:
1485 1345 660 391 267 174 27
EagI C'GGCC_G
Cuts at: 1934 1934
Size: 4349
EarI CTCTTCn'nnn_
Cuts at: 1345 2372 2582 1345
Size: 1027 210 3112
Fragments arranged by size:
3112 1027 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 2557 2867 3013 4159 127
Size: 2430 310 146 1146 317
Fragments arranged by size:
2430 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 510 1188 1361 510
Size: 678 173 3498
Fragments arranged by size:
3498 678 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 701 910 2174 2606 3353 3621 701
Size: 209 1264 432 747 268 1429
Fragments arranged by size:
1429 1264 747 432 268 209
EcoNI CCTnn'n_nnAGG
Cuts at: 631 631
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 168 1488 168
Size: 1320 3029
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
EcoRV GAT'ATC
Cuts at: 307 307
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 927 2368 927
Size: 1441 2908
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 490 992 1783 2031 3053 4032 490
Size: 502 791 248 1022 979 807
Fragments arranged by size:
1022 979 807 791 502 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 824 856 2191 2221 2223 2253 824
Size: 32 1335 30 2 30 2920
Fragments arranged by size:
2920 1335 32 30 30 2
HincII GTy'rAC
Cuts at: 1074 1388 1074
Size: 314 4035
HindIII A'AGCT_T
Cuts at: 444 444
Size: 4349
KasI G'GCGC_C
Cuts at: 988 1779 2027 988
Size: 791 248 3310
Fragments arranged by size:
3310 791 248
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1274 3020 3204 1274
Size: 1746 184 2419
Fragments arranged by size:
2419 1746 184
MscI TGG'CCA
Cuts at: 184 451 2110 184
Size: 267 1659 2423
Fragments arranged by size:
2423 1659 267
MslI CAynn'nnrTG
Cuts at: 1082 1210 1240 2338 2465 1082
Size: 128 30 1098 127 2966
Fragments arranged by size:
2966 1098 128 127 30
MspA1I CmG'CkG
Cuts at: 162 579 782 788 1460 1774 2134 3147 3392
Size: 417 203 6 672 314 360 1013 245
Cuts at: 3392 162
Size: 1119
Fragments arranged by size:
1119 1013 672 417 360 314 245 203
6
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 989 1780 2028 989
Size: 791 248 3310
Fragments arranged by size:
3310 791 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NspI r_CATG'y
Cuts at: 1209 1402 1902 2809 1209
Size: 193 500 907 2749
Fragments arranged by size:
2749 907 500 193
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 168 168
Size: 4349
PstI C_TGCA'G
Cuts at: 133 167 1100 1396 2081 133
Size: 34 933 296 685 2401
Fragments arranged by size:
2401 933 685 296 34
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 162 1774 2134 162
Size: 1612 360 2377
Fragments arranged by size:
2377 1612 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 512 1190 1363 512
Size: 678 173 3498
Fragments arranged by size:
3498 678 173
SacII CC_GC'GG
Cuts at: 580 580
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1100 1396 1100
Size: 296 4053
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SexAI A'CCwGG_T
Cuts at: 287 458 632 287
Size: 171 174 4004
Fragments arranged by size:
4004 174 171
SfcI C'TryA_G
Cuts at: 33 129 163 545 1096 1392 2077 3070 3261
Size: 96 34 382 551 296 685 993 191
Cuts at: 3261 33
Size: 1121
Fragments arranged by size:
1121 993 685 551 382 296 191 96
34
SfoI GGC'GCC
Cuts at: 990 1781 2029 990
Size: 791 248 3310
Fragments arranged by size:
3310 791 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 695 1371 695
Size: 676 3673
SmlI C'TyrA_G
Cuts at: 346 364 1504 2419 2911 3173 346
Size: 18 1140 915 492 262 1522
Fragments arranged by size:
1522 1140 915 492 262 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
SspI AAT'ATT
Cuts at: 283 283
Size: 4349
StyI C'CwwG_G
Cuts at: 107 1483 1837 107
Size: 1376 354 2619
Fragments arranged by size:
2619 1376 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 1932 2065 2524 2733 690
Size: 1242 133 459 209 2306
Fragments arranged by size:
2306 1242 459 209 133
TatI w'GTAC_w
Cuts at: 590 1028 1103 3864 590
Size: 438 75 2761 1075
Fragments arranged by size:
2761 1075 438 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 652 684 3866 3898 652
Size: 32 3182 32 1103
Fragments arranged by size:
3182 1103 32 32
Tth111I GACn'n_nGTC
Cuts at: 1320 2146 1320
Size: 826 3523
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1257 1844 1257
Size: 587 3762
XmaI C'CCGG_G
Cuts at: 693 1369 693
Size: 676 3673
XmnI GAAnn'nnTTC
Cuts at: 910 910
Size: 4349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI DraIII FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI