Restriction map for
pF1KB6613
(Circular) (Six-base) MAPSORT of: pF1KB6613.seq Check: 8259 from: 1 to: 4349
pF1KB6613 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 374 1387 374
Size: 1013 3336
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AleI CACnn'nnGTG
Cuts at: 139 139
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 157 556 157
Size: 399 3950
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1057 1089 1057
Size: 32 4317
AlwNI CAG_nnn'CTG
Cuts at: 1654 3221 1654
Size: 1567 2782
ApaI G_GGCC'C
Cuts at: 1241 1241
Size: 4349
ApaLI G'TGCA_C
Cuts at: 229 1321 3119 229
Size: 1092 1798 1459
Fragments arranged by size:
1798 1459 1092
ApoI r'AATT_y
Cuts at: 214 1353 3702 3958 214
Size: 1139 2349 256 605
Fragments arranged by size:
2349 1139 605 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 129 368 577 714 1294 1369 129
Size: 239 209 137 580 75 3109
Fragments arranged by size:
3109 580 239 209 137 75
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 133 1003 1136 1365 1779 2027 2062 133
Size: 870 133 229 414 248 35 2420
Fragments arranged by size:
2420 870 414 248 229 133 35
BanII G_rGCy'C
Cuts at: 169 369 1241 1363 2393 169
Size: 200 872 122 1030 2125
Fragments arranged by size:
2125 1030 872 200 122
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BciVI GTATCCnnnnn_n'
Cuts at: 513 2247 3008 4349 513
Size: 1734 761 1341 513
Fragments arranged by size:
1734 1341 761 513
BclI T'GATC_A
Cuts at: 762 1869 762
Size: 1107 3242
BglII A'GATC_T
Cuts at: 509 1864 3901 509
Size: 1355 2037 957
Fragments arranged by size:
2037 1355 957
BlpI GC'TnA_GC
Cuts at: 1461 1461
Size: 4349
Bme1580I G_kGCm'C
Cuts at: 136 233 1141 1241 1325 1974 2067 3123 136
Size: 97 908 100 84 649 93 1056 1362
Fragments arranged by size:
1362 1056 908 649 100 97 93 84
BmrI ACTGGGnnnn_n'
Cuts at: 745 1561 1642 1977 4121 745
Size: 816 81 335 2144 973
Fragments arranged by size:
2144 973 816 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 531 2716 531
Size: 2185 2164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 928 565
Size: 363 3986
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1525 2404 2896 3194 1525
Size: 879 492 298 2680
Fragments arranged by size:
2680 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1250 4053 1250
Size: 2803 1546
BsaAI yAC'GTr
Cuts at: 309 744 1702 309
Size: 435 958 2956
Fragments arranged by size:
2956 958 435
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1103 1780 2028 2730 4226 1103
Size: 677 248 702 1496 1226
Fragments arranged by size:
1496 1226 702 677 248
BsaWI w'CCGG_w
Cuts at: 608 929 1538 2059 2737 3011 3158 3569 608
Size: 321 609 521 678 274 147 411 1388
Fragments arranged by size:
1388 678 609 521 411 321 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 280 310 280
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1222 1222
Size: 4349
BseYI C'CCAG_C
Cuts at: 1081 1140 1774 2532 3109 3605 1081
Size: 59 634 758 577 496 1825
Fragments arranged by size:
1825 758 634 577 496 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1360 831
Size: 529 3820
BsiEI CG_ry'CG
Cuts at: 104 125 930 1198 1891 1937 3145 104
Size: 21 805 268 693 46 1208 1308
Fragments arranged by size:
1308 1208 805 693 268 46 21
BsiHKAI G_wGCw'C
Cuts at: 233 407 535 1325 1363 2141 2331 3123 233
Size: 174 128 790 38 778 190 792 1459
Fragments arranged by size:
1459 792 790 778 190 174 128 38
BsmBI CGTCTCn'nnnn_
Cuts at: 832 832
Size: 4349
Bsp1286I G_dGCh'C
Cuts at: 136 169 233 369 407 535 1141 1241 1325
Size: 33 64 136 38 128 606 100 84
Cuts at: 1325 1363 1974 2067 2141 2331 2393 3123 136
Size: 38 611 93 74 190 62 730 1362
Fragments arranged by size:
1362 730 611 606 190 136 128 100
93 84 74 64 62 38 38 33
BspEI T'CCGG_A
Cuts at: 608 3569 608
Size: 2961 1388
BspHI T'CATG_A
Cuts at: 438 3525 438
Size: 3087 1262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 200 1291 1332 1399 1613 1915 2296 200
Size: 1091 41 67 214 302 381 2253
Fragments arranged by size:
2253 1091 381 302 214 67 41
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 623 2261 623
Size: 1638 2711
BsrFI r'CCGG_y
Cuts at: 2347 2528 2737 2347
Size: 181 209 3959
Fragments arranged by size:
3959 209 181
BssSI C'ACGA_G
Cuts at: 2620 2978 2620
Size: 358 3991
BstAPI GCAn_nnn'nTGC
Cuts at: 634 1726 634
Size: 1092 3257
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstXI CCAn_nnnn'nTGG
Cuts at: 894 1185 894
Size: 291 4058
BstYI r'GATC_y
Cuts at: 1 509 1374 1841 1864 2199 2445 3457 3543
Size: 508 865 467 23 335 246 1012 86
Cuts at: 3543 3555 3901 1
Size: 12 346 449
Fragments arranged by size:
1012 865 508 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 137 107
Size: 30 4319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 361 2299 2480 2643 361
Size: 1938 181 163 2067
Fragments arranged by size:
2067 1938 181 163
BtsI GCAGTG_nn'
Cuts at: 1592 1592
Size: 4349
DraI TTT'AAA
Cuts at: 474 1348 474
Size: 874 3475
DraIII CAC_nnn'GTG
Cuts at: 1136 1136
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 319 2055 2913 319
Size: 1736 858 1755
Fragments arranged by size:
1755 1736 858
EaeI y'GGCC_r
Cuts at: 122 155 1934 2108 2499 2526 3871 122
Size: 33 1779 174 391 27 1345 600
Fragments arranged by size:
1779 1345 600 391 174 33 27
EagI C'GGCC_G
Cuts at: 122 1934 122
Size: 1812 2537
EarI CTCTTCn'nnn_
Cuts at: 178 967 2372 2582 178
Size: 789 1405 210 1945
Fragments arranged by size:
1945 1405 789 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2557 2867 3013 4159 2557
Size: 310 146 1146 2747
Fragments arranged by size:
2747 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 928 2174 2606 3353 3621 565
Size: 363 1246 432 747 268 1293
Fragments arranged by size:
1293 1246 747 432 363 268
EcoO109I rG'GnC_Cy
Cuts at: 1488 1488
Size: 4349
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 978 2368 264
Size: 714 1390 2245
Fragments arranged by size:
2245 1390 714
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 1783 2031 3053 4032 1783
Size: 248 1022 979 2100
Fragments arranged by size:
2100 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 811 843 2191 2221 2223 2253 811
Size: 32 1348 30 2 30 2907
Fragments arranged by size:
2907 1348 32 30 30 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
HindIII A'AGCT_T
Cuts at: 423 423
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 899 1178 3020 3204 899
Size: 279 1842 184 2044
Fragments arranged by size:
2044 1842 279 184
MscI TGG'CCA
Cuts at: 2110 2110
Size: 4349
MslI CAynn'nnrTG
Cuts at: 139 538 1162 1316 2338 2465 139
Size: 399 624 154 1022 127 2023
Fragments arranged by size:
2023 1022 624 399 154 127
MspA1I CmG'CkG
Cuts at: 139 1460 1774 2134 3147 3392 139
Size: 1321 314 360 1013 245 1096
Fragments arranged by size:
1321 1096 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2530 2530
Size: 4349
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NcoI C'CATG_G
Cuts at: 107 107
Size: 4349
NgoMIV G'CCGG_C
Cuts at: 2528 2528
Size: 4349
NotI GC'GGCC_GC
Cuts at: 122 122
Size: 4349
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PmlI CAC'GTG
Cuts at: 309 309
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PspOMI G'GGCC_C
Cuts at: 1237 1237
Size: 4349
PspXI vC'TCGA_Gb
Cuts at: 368 368
Size: 4349
PstI C_TGCA'G
Cuts at: 197 1396 2081 197
Size: 1199 685 2465
Fragments arranged by size:
2465 1199 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 1774 2134 1774
Size: 360 3989
RsrII CG'GwC_CG
Cuts at: 870 2544 870
Size: 1674 2675
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SacII CC_GC'GG
Cuts at: 140 140
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 178 2372 2582 178
Size: 2194 210 1945
Fragments arranged by size:
2194 1945 210
SbfI CC_TGCA'GG
Cuts at: 1396 1396
Size: 4349
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SexAI A'CCwGG_T
Cuts at: 221 221
Size: 4349
SfcI C'TryA_G
Cuts at: 33 193 1392 2077 3070 3261 33
Size: 160 1199 685 993 191 1121
Fragments arranged by size:
1199 1121 993 685 191 160
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 131 1296 1371 131
Size: 1165 75 3109
Fragments arranged by size:
3109 1165 75
SmlI C'TyrA_G
Cuts at: 368 1504 2419 2911 3173 368
Size: 1136 915 492 262 1544
Fragments arranged by size:
1544 1136 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StuI AGG'CCT
Cuts at: 295 295
Size: 4349
StyI C'CwwG_G
Cuts at: 107 1178 1483 1837 107
Size: 1071 305 354 2619
Fragments arranged by size:
2619 1071 354 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1932 2065 2524 2733 1932
Size: 133 459 209 3548
Fragments arranged by size:
3548 459 209 133
TatI w'GTAC_w
Cuts at: 710 1121 3864 710
Size: 411 2743 1195
Fragments arranged by size:
2743 1195 411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 306 831 1248 1844 306
Size: 525 417 596 2811
Fragments arranged by size:
2811 596 525 417
XhoI C'TCGA_G
Cuts at: 368 368
Size: 4349
XmaI C'CCGG_G
Cuts at: 129 1294 1369 129
Size: 1165 75 3109
Fragments arranged by size:
3109 1165 75
XmnI GAAnn'nnTTC
Cuts at: 961 961
Size: 4349
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PmeI
PmlI PpiI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PflMI PfoI PpuMI
PshAI PsiI PsrI SanDI SfiI SgrAI SnaBI SrfI
SspI SwaI ZraI