Restriction map for
pF1KB6614
(Circular) (Six-base) MAPSORT of: pF1KB6614.seq Check: 4687 from: 1 to: 4409
pF1KB6614 4409 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1447 1447
Size: 4409
Acc65I G'GTAC_C
Cuts at: 464 1425 464
Size: 961 3448
AclI AA'CG_TT
Cuts at: 4249 4249
Size: 4409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 918 2234 2666 3413 3681 781
Size: 137 1316 432 747 268 1509
Fragments arranged by size:
1509 1316 747 432 268 137
AflIII A'CryG_T
Cuts at: 1761 2865 3842 1761
Size: 1104 977 2328
Fragments arranged by size:
2328 1104 977
AgeI A'CCGG_T
Cuts at: 1311 2797 1311
Size: 1486 2923
AhdI GACnn_n'nnGTC
Cuts at: 1322 1322
Size: 4409
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1265 1265
Size: 4409
AlwNI CAG_nnn'CTG
Cuts at: 642 1140 1714 3281 642
Size: 498 574 1567 1770
Fragments arranged by size:
1770 1567 574 498
ApaI G_GGCC'C
Cuts at: 488 488
Size: 4409
ApaLI G'TGCA_C
Cuts at: 3179 3179
Size: 4409
ApoI r'AATT_y
Cuts at: 1413 3762 4018 1413
Size: 2349 256 1804
Fragments arranged by size:
2349 1804 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4409
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
AvaI C'yCGr_G
Cuts at: 262 480 1429 262
Size: 218 949 3242
Fragments arranged by size:
3242 949 218
BamHI G'GATC_C
Cuts at: 1434 1434
Size: 4409
BanI G'GyrC_C
Cuts at: 214 464 1425 1839 2087 2122 214
Size: 250 961 414 248 35 2501
Fragments arranged by size:
2501 961 414 250 248 35
BanII G_rGCy'C
Cuts at: 157 488 1016 1423 2453 157
Size: 331 528 407 1030 2113
Fragments arranged by size:
2113 1030 528 407 331
BbeI G_GCGC'C
Cuts at: 1843 2091 1843
Size: 248 4161
BbsI GAAGACnn'nnnn_
Cuts at: 1234 1234
Size: 4409
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 243 277 243
Size: 34 4375
BciVI GTATCCnnnnn_n'
Cuts at: 2307 3068 4409 2307
Size: 761 1341 2307
Fragments arranged by size:
2307 1341 761
BclI T'GATC_A
Cuts at: 1929 1929
Size: 4409
BglI GCCn_nnn'nGGC
Cuts at: 170 598 170
Size: 428 3981
BglII A'GATC_T
Cuts at: 1004 1924 3961 1004
Size: 920 2037 1452
Fragments arranged by size:
2037 1452 920
BlpI GC'TnA_GC
Cuts at: 1060 1521 1060
Size: 461 3948
Bme1580I G_kGCm'C
Cuts at: 420 488 2034 2127 3183 420
Size: 68 1546 93 1056 1646
Fragments arranged by size:
1646 1546 1056 93 68
BmrI ACTGGGnnnn_n'
Cuts at: 1621 1702 2037 4181 1621
Size: 81 335 2144 1849
Fragments arranged by size:
2144 1849 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2776 2776
Size: 4409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 264 371 442 463 739 1099 1258 1291
Size: 141 107 71 21 276 360 159 33
Cuts at: 1291 123
Size: 3241
Fragments arranged by size:
3241 360 276 159 141 107 71 33
21
Bpu10I CC'TnA_GC
Cuts at: 853 853
Size: 4409
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 1585 2464 2956 3254 724
Size: 861 879 492 298 1879
Fragments arranged by size:
1879 879 861 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4113 4113
Size: 4409
BsaAI yAC'GTr
Cuts at: 1762 1762
Size: 4409
BsaBI GATnn'nnATC
Cuts at: 6 1923 3960 6
Size: 1917 2037 455
Fragments arranged by size:
2037 1917 455
BsaHI Gr'CG_yC
Cuts at: 1840 2088 2790 4286 1840
Size: 248 702 1496 1963
Fragments arranged by size:
1963 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1311 1598 2119 2797 3071 3218 3629 1311
Size: 287 521 678 274 147 411 2091
Fragments arranged by size:
2091 678 521 411 287 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 126 147 439 538 760 790 793 1171 1362
Size: 21 292 99 222 30 3 378 191
Cuts at: 1362 126
Size: 3173
Fragments arranged by size:
3173 378 292 222 191 99 30 21
3
BseYI C'CCAG_C
Cuts at: 231 1055 1169 1834 2592 3169 3665 231
Size: 824 114 665 758 577 496 975
Fragments arranged by size:
975 824 758 665 577 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1138 1006
Size: 132 4277
BsiEI CG_ry'CG
Cuts at: 104 1951 1997 3205 104
Size: 1847 46 1208 1308
Fragments arranged by size:
1847 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1016 1423 2201 2391 3183 1016
Size: 407 778 190 792 2242
Fragments arranged by size:
2242 792 778 407 190
Bsp1286I G_dGCh'C
Cuts at: 157 420 488 1016 1423 2034 2127 2201 2391
Size: 263 68 528 407 611 93 74 190
Cuts at: 2391 2453 3183 157
Size: 62 730 1383
Fragments arranged by size:
1383 730 611 528 407 263 190 93
74 68 62
BspEI T'CCGG_A
Cuts at: 3629 3629
Size: 4409
BspHI T'CATG_A
Cuts at: 3585 3585
Size: 4409
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1374 1459 1673 1975 2356 1374
Size: 85 214 302 381 3427
Fragments arranged by size:
3427 381 302 214 85
BsrBI CCG'CTC
Cuts at: 50 2701 2755 4239 4404 50
Size: 2651 54 1484 165 55
Fragments arranged by size:
2651 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2321 2321
Size: 4409
BsrFI r'CCGG_y
Cuts at: 194 217 1311 2407 2588 2797 194
Size: 23 1094 1096 181 209 1806
Fragments arranged by size:
1806 1096 1094 209 181 23
BssSI C'ACGA_G
Cuts at: 772 2680 3038 772
Size: 1908 358 2143
Fragments arranged by size:
2143 1908 358
BstAPI GCAn_nnn'nTGC
Cuts at: 191 642 1786 191
Size: 451 1144 2814
Fragments arranged by size:
2814 1144 451
BstBI TT'CG_AA
Cuts at: 2770 2770
Size: 4409
BstEII G'GTnAC_C
Cuts at: 1314 1314
Size: 4409
BstXI CCAn_nnnn'nTGG
Cuts at: 808 808
Size: 4409
BstYI r'GATC_y
Cuts at: 1 235 452 539 1004 1130 1434 1901 1924
Size: 234 217 87 465 126 304 467 23
Cuts at: 1924 2259 2505 3517 3603 3615 3961 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 465 449 346 335 304 246
234 217 126 87 86 23 12
BtgI C'CryG_G
Cuts at: 554 554
Size: 4409
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2359 2540 2703 2359
Size: 181 163 4065
Fragments arranged by size:
4065 181 163
BtsI GCAGTG_nn'
Cuts at: 1652 1652
Size: 4409
DraI TTT'AAA
Cuts at: 1408 1408
Size: 4409
DrdI GACnn_nn'nnGTC
Cuts at: 2115 2973 2115
Size: 858 3551
EaeI y'GGCC_r
Cuts at: 704 965 1994 2168 2559 2586 3931 704
Size: 261 1029 174 391 27 1345 1182
Fragments arranged by size:
1345 1182 1029 391 261 174 27
EagI C'GGCC_G
Cuts at: 1994 1994
Size: 4409
EarI CTCTTCn'nnn_
Cuts at: 1340 2432 2642 1340
Size: 1092 210 3107
Fragments arranged by size:
3107 1092 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 112 1108 2617 2927 3073 4219 112
Size: 996 1509 310 146 1146 302
Fragments arranged by size:
1509 1146 996 310 302 146
EcoICRI GAG'CTC
Cuts at: 1014 1421 1014
Size: 407 4002
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 123 264 371 442 463 739 781 918 1099
Size: 141 107 71 21 276 42 137 181
Cuts at: 1099 1258 1291 2234 2666 3413 3681 123
Size: 159 33 943 432 747 268 851
Fragments arranged by size:
943 851 747 432 276 268 181 159
141 137 107 71 42 33 21
EcoNI CCTnn'n_nnAGG
Cuts at: 406 424 742 1270 406
Size: 18 318 528 3545
Fragments arranged by size:
3545 528 318 18
EcoO109I rG'GnC_Cy
Cuts at: 477 484 639 1548 477
Size: 7 155 909 3338
Fragments arranged by size:
3338 909 155 7
EcoRI G'AATT_C
Cuts at: 1413 1413
Size: 4409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4409
FspI TGC'GCA
Cuts at: 679 2190 679
Size: 1511 2898
HaeII r_GCGC'y
Cuts at: 1843 2091 3113 4092 1843
Size: 248 1022 979 2160
Fragments arranged by size:
2160 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1036 1068 1308 1340 2251 2281 2283 2313 1036
Size: 32 240 32 911 30 2 30 3132
Fragments arranged by size:
3132 911 240 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1448 1448
Size: 4409
KasI G'GCGC_C
Cuts at: 1839 2087 1839
Size: 248 4161
KpnI G_GTAC'C
Cuts at: 468 1429 468
Size: 961 3448
MfeI C'AATT_G
Cuts at: 518 518
Size: 4409
MluI A'CGCG_T
Cuts at: 3842 3842
Size: 4409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3080 3264 3080
Size: 184 4225
MscI TGG'CCA
Cuts at: 706 967 2170 706
Size: 261 1203 2945
Fragments arranged by size:
2945 1203 261
MslI CAynn'nnrTG
Cuts at: 2398 2525 2398
Size: 127 4282
MspA1I CmG'CkG
Cuts at: 284 1122 1164 1520 1834 2194 3207 3452 284
Size: 838 42 356 314 360 1013 245 1241
Fragments arranged by size:
1241 1013 838 360 356 314 245 42
NaeI GCC'GGC
Cuts at: 196 219 2590 196
Size: 23 2371 2015
Fragments arranged by size:
2371 2015 23
NarI GG'CG_CC
Cuts at: 1840 2088 1840
Size: 248 4161
NgoMIV G'CCGG_C
Cuts at: 194 217 2588 194
Size: 23 2371 2015
Fragments arranged by size:
2371 2015 23
NspI r_CATG'y
Cuts at: 1462 1962 2869 1462
Size: 500 907 3002
Fragments arranged by size:
3002 907 500
PciI A'CATG_T
Cuts at: 2865 2865
Size: 4409
PfoI T'CCnGG_A
Cuts at: 1352 1352
Size: 4409
PmeI GTTT'AAAC
Cuts at: 1408 1408
Size: 4409
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 913 945 3548 3580 913
Size: 32 2603 32 1742
Fragments arranged by size:
2603 1742 32 32
PspOMI G'GGCC_C
Cuts at: 484 484
Size: 4409
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1189 1221 1189
Size: 32 4377
PstI C_TGCA'G
Cuts at: 229 533 1052 1456 2141 229
Size: 304 519 404 685 2497
Fragments arranged by size:
2497 685 519 404 304
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4409
PvuII CAG'CTG
Cuts at: 284 1122 1164 1834 2194 284
Size: 838 42 670 360 2499
Fragments arranged by size:
2499 838 670 360 42
RsrII CG'GwC_CG
Cuts at: 2604 2604
Size: 4409
SacI G_AGCT'C
Cuts at: 1016 1423 1016
Size: 407 4002
SalI G'TCGA_C
Cuts at: 1446 1446
Size: 4409
SapI GCTCTTCn'nnn_
Cuts at: 2432 2642 2432
Size: 210 4199
SbfI CC_TGCA'GG
Cuts at: 229 1456 229
Size: 1227 3182
ScaI AGT'ACT
Cuts at: 508 3926 508
Size: 3418 991
SfcI C'TryA_G
Cuts at: 33 225 288 498 510 529 577 1048 1452
Size: 192 63 210 12 19 48 471 404
Cuts at: 1452 2137 3130 3321 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 471 404 210 192 191
63 48 19 12
SfoI GGC'GCC
Cuts at: 1841 2089 1841
Size: 248 4161
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4409
SmaI CCC'GGG
Cuts at: 482 1431 482
Size: 949 3460
SmlI C'TyrA_G
Cuts at: 739 1564 2479 2971 3233 739
Size: 825 915 492 262 1915
Fragments arranged by size:
1915 915 825 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4409
SphI G_CATG'C
Cuts at: 1462 1962 1462
Size: 500 3909
StuI AGG'CCT
Cuts at: 1033 1033
Size: 4409
StyI C'CwwG_G
Cuts at: 1034 1543 1897 1034
Size: 509 354 3546
Fragments arranged by size:
3546 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 202 796 1992 2125 2584 2793 202
Size: 594 1196 133 459 209 1818
Fragments arranged by size:
1818 1196 594 459 209 133
TatI w'GTAC_w
Cuts at: 506 1298 3924 506
Size: 792 2626 991
Fragments arranged by size:
2626 991 792
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3926 3958 3926
Size: 32 4377
Tth111I GACn'n_nGTC
Cuts at: 2206 2206
Size: 4409
XbaI T'CTAG_A
Cuts at: 1440 1440
Size: 4409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 438 714 978 1032 1141 1904 438
Size: 276 264 54 109 763 2943
Fragments arranged by size:
2943 763 276 264 109 54
XmaI C'CCGG_G
Cuts at: 480 1429 480
Size: 949 3460
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PspOMI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PpuMI
PshAI PsiI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI