Restriction map for
pF1KB6615
(Circular) (Six-base) MAPSORT of: pF1KB6615.seq Check: 3840 from: 1 to: 4349
pF1KB6615 4349 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 513 513
Size: 4349
AccI GT'mk_AC
Cuts at: 1387 1387
Size: 4349
Acc65I G'GTAC_C
Cuts at: 1365 1365
Size: 4349
AclI AA'CG_TT
Cuts at: 4189 4189
Size: 4349
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2174 2606 3353 3621 2174
Size: 432 747 268 2902
Fragments arranged by size:
2902 747 432 268
AflIII A'CryG_T
Cuts at: 1701 2805 3782 1701
Size: 1104 977 2268
Fragments arranged by size:
2268 1104 977
AgeI A'CCGG_T
Cuts at: 2737 2737
Size: 4349
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 4349
AlwNI CAG_nnn'CTG
Cuts at: 156 486 1654 3221 156
Size: 330 1168 1567 1284
Fragments arranged by size:
1567 1284 1168 330
ApaI G_GGCC'C
Cuts at: 279 1167 279
Size: 888 3461
ApaLI G'TGCA_C
Cuts at: 3119 3119
Size: 4349
ApoI r'AATT_y
Cuts at: 1353 3702 3958 1353
Size: 2349 256 1744
Fragments arranged by size:
2349 1744 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
AvaI C'yCGr_G
Cuts at: 850 979 1369 850
Size: 129 390 3830
Fragments arranged by size:
3830 390 129
BamHI G'GATC_C
Cuts at: 1374 1374
Size: 4349
BanI G'GyrC_C
Cuts at: 439 799 1365 1779 2027 2062 439
Size: 360 566 414 248 35 2726
Fragments arranged by size:
2726 566 414 360 248 35
BanII G_rGCy'C
Cuts at: 170 279 301 1167 1363 2393 170
Size: 109 22 866 196 1030 2126
Fragments arranged by size:
2126 1030 866 196 109 22
BbeI G_GCGC'C
Cuts at: 1783 2031 1783
Size: 248 4101
BbsI GAAGACnn'nnnn_
Cuts at: 1002 1002
Size: 4349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1323 1357 90
Size: 34 1199 34 3082
Fragments arranged by size:
3082 1199 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2247 3008 4349 2247
Size: 761 1341 2247
Fragments arranged by size:
2247 1341 761
BclI T'GATC_A
Cuts at: 1869 1869
Size: 4349
BglI GCCn_nnn'nGGC
Cuts at: 165 409 983 1162 1171 165
Size: 244 574 179 9 3343
Fragments arranged by size:
3343 574 244 179 9
BglII A'GATC_T
Cuts at: 1864 3901 1864
Size: 2037 2312
BlpI GC'TnA_GC
Cuts at: 1075 1461 1075
Size: 386 3963
Bme1580I G_kGCm'C
Cuts at: 279 1167 1231 1974 2067 3123 279
Size: 888 64 743 93 1056 1505
Fragments arranged by size:
1505 1056 888 743 93 64
BmgBI CAC'GTC
Cuts at: 732 732
Size: 4349
BmrI ACTGGGnnnn_n'
Cuts at: 511 1139 1561 1642 1977 4121 511
Size: 628 422 81 335 2144 739
Fragments arranged by size:
2144 739 628 422 335 81
BmtI G_CTAG'C
Cuts at: 556 739 556
Size: 183 4166
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 2716
Size: 4349
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 398 508 1222 154
Size: 244 110 714 3281
Fragments arranged by size:
3281 714 244 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 247 1077 1525 2404 2896 3194 247
Size: 830 448 879 492 298 1402
Fragments arranged by size:
1402 879 830 492 448 298
BsaI GGTCTCn'nnnn_
Cuts at: 4053 4053
Size: 4349
BsaAI yAC'GTr
Cuts at: 1702 1702
Size: 4349
BsaBI GATnn'nnATC
Cuts at: 6 1863 3900 6
Size: 1857 2037 455
Fragments arranged by size:
2037 1857 455
BsaHI Gr'CG_yC
Cuts at: 1780 2028 2730 4226 1780
Size: 248 702 1496 1903
Fragments arranged by size:
1903 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1538 2059 2737 3011 3158 3569 1538
Size: 521 678 274 147 411 2318
Fragments arranged by size:
2318 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1094 1124 1094
Size: 30 4319
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 894 894
Size: 4349
BseYI C'CCAG_C
Cuts at: 540 556 786 1209 1224 1330 1774 2532 3109
Size: 16 230 423 15 106 444 758 577
Cuts at: 3109 3605 540
Size: 496 1284
Fragments arranged by size:
1284 758 577 496 444 423 230 106
16 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 499 541 641 169
Size: 330 42 100 3877
Fragments arranged by size:
3877 330 100 42
BsiEI CG_ry'CG
Cuts at: 104 497 1891 1937 3145 104
Size: 393 1394 46 1208 1308
Fragments arranged by size:
1394 1308 1208 393 46
BsiHKAI G_wGCw'C
Cuts at: 776 1363 2141 2331 3123 776
Size: 587 778 190 792 2002
Fragments arranged by size:
2002 792 778 587 190
Bsp1286I G_dGCh'C
Cuts at: 170 279 301 776 1167 1231 1363 1974 2067
Size: 109 22 475 391 64 132 611 93
Cuts at: 2067 2141 2331 2393 3123 170
Size: 74 190 62 730 1396
Fragments arranged by size:
1396 730 611 475 391 190 132 109
93 74 64 62 22
BspEI T'CCGG_A
Cuts at: 3569 3569
Size: 4349
BspHI T'CATG_A
Cuts at: 3525 3525
Size: 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 513 1399 1613 1915 2296 513
Size: 886 214 302 381 2566
Fragments arranged by size:
2566 886 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2641 2695 4179 4344 50
Size: 2591 54 1484 165 55
Fragments arranged by size:
2591 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2261 2261
Size: 4349
BsrFI r'CCGG_y
Cuts at: 133 202 492 2347 2528 2737 133
Size: 69 290 1855 181 209 1745
Fragments arranged by size:
1855 1745 290 209 181 69
BssHII G'CGCG_C
Cuts at: 563 563
Size: 4349
BssSI C'ACGA_G
Cuts at: 591 2620 2978 591
Size: 2029 358 1962
Fragments arranged by size:
2029 1962 358
BstAPI GCAn_nnn'nTGC
Cuts at: 156 723 1726 156
Size: 567 1003 2779
Fragments arranged by size:
2779 1003 567
BstBI TT'CG_AA
Cuts at: 2710 2710
Size: 4349
BstEII G'GTnAC_C
Cuts at: 1110 1110
Size: 4349
BstYI r'GATC_y
Cuts at: 1 1374 1841 1864 2199 2445 3457 3543 3555
Size: 1373 467 23 335 246 1012 86 12
Cuts at: 3555 3901 1
Size: 346 449
Fragments arranged by size:
1373 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 253 638 834 253
Size: 385 196 3768
Fragments arranged by size:
3768 385 196
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 879 2299 2480 2643 879
Size: 1420 181 163 2585
Fragments arranged by size:
2585 1420 181 163
BtsI GCAGTG_nn'
Cuts at: 1592 1592
Size: 4349
DraI TTT'AAA
Cuts at: 1348 1348
Size: 4349
DrdI GACnn_nn'nnGTC
Cuts at: 2055 2913 2055
Size: 858 3491
EaeI y'GGCC_r
Cuts at: 131 233 384 494 595 635 926 1193 1235
Size: 102 151 110 101 40 291 267 42
Cuts at: 1235 1934 2108 2499 2526 3871 131
Size: 699 174 391 27 1345 609
Fragments arranged by size:
1345 699 609 391 291 267 174 151
110 102 101 42 40 27
EagI C'GGCC_G
Cuts at: 494 1934 494
Size: 1440 2909
EarI CTCTTCn'nnn_
Cuts at: 824 2372 2582 824
Size: 1548 210 2591
Fragments arranged by size:
2591 1548 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 964 2557 2867 3013 4159 964
Size: 1593 310 146 1146 1154
Fragments arranged by size:
1593 1154 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1361 1361
Size: 4349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 154 398 508 1222 2174 2606 3353 3621 154
Size: 244 110 714 952 432 747 268 882
Fragments arranged by size:
952 882 747 714 432 268 244 110
EcoNI CCTnn'n_nnAGG
Cuts at: 148 148
Size: 4349
EcoO109I rG'GnC_Cy
Cuts at: 153 276 308 917 984 1163 1164 1488 153
Size: 123 32 609 67 179 1 324 3014
Fragments arranged by size:
3014 609 324 179 123 67 32 1
EcoRI G'AATT_C
Cuts at: 1353 1353
Size: 4349
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2368 2368
Size: 4349
FseI GG_CCGG'CC
Cuts at: 137 137
Size: 4349
FspI TGC'GCA
Cuts at: 2130 2130
Size: 4349
HaeII r_GCGC'y
Cuts at: 577 1783 2031 3053 4032 577
Size: 1206 248 1022 979 894
Fragments arranged by size:
1206 1022 979 894 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1068 1094 1100 1126 2191 2221 2223 2253 1068
Size: 26 6 26 1065 30 2 30 3164
Fragments arranged by size:
3164 1065 30 30 26 26 6 2
HincII GTy'rAC
Cuts at: 1388 1388
Size: 4349
KasI G'GCGC_C
Cuts at: 1779 2027 1779
Size: 248 4101
KpnI G_GTAC'C
Cuts at: 1369 1369
Size: 4349
MluI A'CGCG_T
Cuts at: 3782 3782
Size: 4349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3020 3204 3020
Size: 184 4165
MscI TGG'CCA
Cuts at: 235 386 928 1195 2110 235
Size: 151 542 267 915 2474
Fragments arranged by size:
2474 915 542 267 151
MslI CAynn'nnrTG
Cuts at: 1132 2338 2465 1132
Size: 1206 127 3016
Fragments arranged by size:
3016 1206 127
MspA1I CmG'CkG
Cuts at: 129 282 406 483 640 1152 1460 1774 2134
Size: 153 124 77 157 512 308 314 360
Cuts at: 2134 3147 3392 129
Size: 1013 245 1086
Fragments arranged by size:
1086 1013 512 360 314 308 245 157
153 124 77
NaeI GCC'GGC
Cuts at: 135 204 494 2530 135
Size: 69 290 2036 1954
Fragments arranged by size:
2036 1954 290 69
NarI GG'CG_CC
Cuts at: 1780 2028 1780
Size: 248 4101
NgoMIV G'CCGG_C
Cuts at: 133 202 492 2528 133
Size: 69 290 2036 1954
Fragments arranged by size:
2036 1954 290 69
NheI G'CTAG_C
Cuts at: 552 735 552
Size: 183 4166
NspI r_CATG'y
Cuts at: 1402 1902 2809 1402
Size: 500 907 2942
Fragments arranged by size:
2942 907 500
PasI CC'CwG_GG
Cuts at: 909 1026 1160 909
Size: 117 134 4098
Fragments arranged by size:
4098 134 117
PciI A'CATG_T
Cuts at: 2805 2805
Size: 4349
PflMI CCAn_nnn'nTGG
Cuts at: 629 935 629
Size: 306 4043
PfoI T'CCnGG_A
Cuts at: 375 375
Size: 4349
PmeI GTTT'AAAC
Cuts at: 1348 1348
Size: 4349
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3488 3520 3488
Size: 32 4317
PpuMI rG'GwC_Cy
Cuts at: 917 917
Size: 4349
PspOMI G'GGCC_C
Cuts at: 275 1163 275
Size: 888 3461
PstI C_TGCA'G
Cuts at: 1091 1147 1172 1396 2081 1091
Size: 56 25 224 685 3359
Fragments arranged by size:
3359 685 224 56 25
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4349
PvuII CAG'CTG
Cuts at: 483 1152 1774 2134 483
Size: 669 622 360 2698
Fragments arranged by size:
2698 669 622 360
RsrII CG'GwC_CG
Cuts at: 2544 2544
Size: 4349
SacI G_AGCT'C
Cuts at: 1363 1363
Size: 4349
SacII CC_GC'GG
Cuts at: 641 641
Size: 4349
SalI G'TCGA_C
Cuts at: 1386 1386
Size: 4349
SapI GCTCTTCn'nnn_
Cuts at: 2372 2582 2372
Size: 210 4139
SbfI CC_TGCA'GG
Cuts at: 1172 1396 1172
Size: 224 4125
ScaI AGT'ACT
Cuts at: 3866 3866
Size: 4349
SexAI A'CCwGG_T
Cuts at: 143 143
Size: 4349
SfcI C'TryA_G
Cuts at: 33 1087 1143 1168 1182 1392 2077 3070 3261
Size: 1054 56 25 14 210 685 993 191
Cuts at: 3261 33
Size: 1121
Fragments arranged by size:
1121 1054 993 685 210 191 56 25
14
SfiI GGCCn_nnn'nGGCC
Cuts at: 1162 1171 1162
Size: 9 4340
SfoI GGC'GCC
Cuts at: 1781 2029 1781
Size: 248 4101
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4349
SmaI CCC'GGG
Cuts at: 1371 1371
Size: 4349
SmlI C'TyrA_G
Cuts at: 262 1056 1504 2419 2911 3173 262
Size: 794 448 915 492 262 1438
Fragments arranged by size:
1438 915 794 492 448 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4349
SphI G_CATG'C
Cuts at: 1402 1902 1402
Size: 500 3849
StuI AGG'CCT
Cuts at: 1106 1244 1106
Size: 138 4211
StyI C'CwwG_G
Cuts at: 236 1004 1483 1837 236
Size: 768 479 354 2748
Fragments arranged by size:
2748 768 479 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 303 427 778 865 901 1344 1932 2065 2524
Size: 124 351 87 36 443 588 133 459
Cuts at: 2524 2733 303
Size: 209 1919
Fragments arranged by size:
1919 588 459 443 351 209 133 124
87 36
TatI w'GTAC_w
Cuts at: 3864 3864
Size: 4349
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4317
Tth111I GACn'n_nGTC
Cuts at: 2146 2146
Size: 4349
XbaI T'CTAG_A
Cuts at: 1380 1380
Size: 4349
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 453 630 1844 453
Size: 177 1214 2958
Fragments arranged by size:
2958 1214 177
XmaI C'CCGG_G
Cuts at: 1369 1369
Size: 4349
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbvCI BfrBI Bpu10I BsiWI BsmI BsmBI BsrGI
BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI
HindIII HpaI MfeI NcoI NdeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SanDI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI