Restriction map for
pF1KB6618
(Circular) (Six-base) MAPSORT of: pF1KB6618.seq Check: 216 from: 1 to: 4412
pF1KB6618 4412 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 591 591
Size: 4412
AccI GT'mk_AC
Cuts at: 1450 1450
Size: 4412
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 1406 4252 1406
Size: 2846 1566
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2237 2669 3416 3684 2237
Size: 432 747 268 2965
Fragments arranged by size:
2965 747 432 268
AflIII A'CryG_T
Cuts at: 1764 2868 3845 1764
Size: 1104 977 2331
Fragments arranged by size:
2331 1104 977
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AleI CACnn'nnGTG
Cuts at: 1233 1233
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 1282 1717 3284 1282
Size: 435 1567 2410
Fragments arranged by size:
2410 1567 435
ApaLI G'TGCA_C
Cuts at: 3182 3182
Size: 4412
ApoI r'AATT_y
Cuts at: 551 1132 1372 1416 3765 4021 551
Size: 581 240 44 2349 256 942
Fragments arranged by size:
2349 942 581 256 240 44
AseI AT'TA_AT
Cuts at: 20 284 20
Size: 264 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1040 1432 1040
Size: 392 4020
BamHI G'GATC_C
Cuts at: 1089 1437 1089
Size: 348 4064
BanI G'GyrC_C
Cuts at: 124 495 1428 1842 2090 2125 124
Size: 371 933 414 248 35 2411
Fragments arranged by size:
2411 933 414 371 248 35
BanII G_rGCy'C
Cuts at: 180 293 870 957 1426 2456 180
Size: 113 577 87 469 1030 2136
Fragments arranged by size:
2136 1030 577 469 113 87
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 145 145
Size: 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4378
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 486 1013 1932 486
Size: 527 919 2966
Fragments arranged by size:
2966 919 527
BglII A'GATC_T
Cuts at: 1927 3964 1927
Size: 2037 2375
BlpI GC'TnA_GC
Cuts at: 1524 1524
Size: 4412
Bme1580I G_kGCm'C
Cuts at: 2037 2130 3186 2037
Size: 93 1056 3263
Fragments arranged by size:
3263 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1624 1705 2040 4184 1624
Size: 81 335 2144 1852
Fragments arranged by size:
2144 1852 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 995 352
Size: 643 3769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1588 2467 2959 3257 259
Size: 1329 879 492 298 1414
Fragments arranged by size:
1414 1329 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4116 4116
Size: 4412
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1601 2122 2800 3074 3221 3632 1601
Size: 521 678 274 147 411 2381
Fragments arranged by size:
2381 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1376 1376
Size: 4412
BseYI C'CCAG_C
Cuts at: 1837 2595 3172 3668 1837
Size: 758 577 496 2581
Fragments arranged by size:
2581 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1257 1012
Size: 245 4167
BsiEI CG_ry'CG
Cuts at: 104 1954 2000 3208 104
Size: 1850 46 1208 1308
Fragments arranged by size:
1850 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 180 226 293 1305 1426 2204 2394 3186 180
Size: 46 67 1012 121 778 190 792 1406
Fragments arranged by size:
1406 1012 792 778 190 121 67 46
Bsp1286I G_dGCh'C
Cuts at: 180 226 293 870 957 1305 1426 2037 2130
Size: 46 67 577 87 348 121 611 93
Cuts at: 2130 2204 2394 2456 3186 180
Size: 74 190 62 730 1406
Fragments arranged by size:
1406 730 611 577 348 190 121 93
87 74 67 62 46
BspEI T'CCGG_A
Cuts at: 3632 3632
Size: 4412
BspHI T'CATG_A
Cuts at: 3588 3588
Size: 4412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 591 1462 1676 1978 2359 591
Size: 871 214 302 381 2644
Fragments arranged by size:
2644 871 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 562 2324 562
Size: 1762 2650
BsrFI r'CCGG_y
Cuts at: 2410 2591 2800 2410
Size: 181 209 4022
Fragments arranged by size:
4022 209 181
BsrGI T'GTAC_A
Cuts at: 400 843 400
Size: 443 3969
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 1789 1789
Size: 4412
BstBI TT'CG_AA
Cuts at: 2773 2773
Size: 4412
BstXI CCAn_nnnn'nTGG
Cuts at: 338 471 338
Size: 133 4279
BstYI r'GATC_y
Cuts at: 1 832 1089 1437 1904 1927 2262 2508 3520
Size: 831 257 348 467 23 335 246 1012
Cuts at: 3520 3606 3618 3964 1
Size: 86 12 346 449
Fragments arranged by size:
1012 831 467 449 348 346 335 257
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 1655 1655
Size: 4412
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DraIII CAC_nnn'GTG
Cuts at: 781 781
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 2118 2976 2118
Size: 858 3554
EaeI y'GGCC_r
Cuts at: 896 1064 1997 2171 2562 2589 3934 896
Size: 168 933 174 391 27 1345 1374
Fragments arranged by size:
1374 1345 933 391 174 168 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 602 2435 2645 602
Size: 1833 210 2369
Fragments arranged by size:
2369 1833 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 2930 3076 4222 2620
Size: 310 146 1146 2810
Fragments arranged by size:
2810 1146 310 146
EcoICRI GAG'CTC
Cuts at: 178 291 1424 178
Size: 113 1133 3166
Fragments arranged by size:
3166 1133 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 995 2237 2669 3416 3684 352
Size: 643 1242 432 747 268 1080
Fragments arranged by size:
1242 1080 747 643 432 268
EcoO109I rG'GnC_Cy
Cuts at: 429 1551 429
Size: 1122 3290
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
EcoRV GAT'ATC
Cuts at: 1209 1209
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 934 1350 2431 934
Size: 416 1081 2915
Fragments arranged by size:
2915 1081 416
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3116 4095 1846
Size: 248 1022 979 2163
Fragments arranged by size:
2163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1167 1199 2254 2284 2286 2316 1167
Size: 32 1055 30 2 30 3263
Fragments arranged by size:
3263 1055 32 30 30 2
HincII GTy'rAC
Cuts at: 1451 1451
Size: 4412
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 986 1138 3083 3267 986
Size: 152 1945 184 2131
Fragments arranged by size:
2131 1945 184 152
MscI TGG'CCA
Cuts at: 898 1066 2173 898
Size: 168 1107 3137
Fragments arranged by size:
3137 1107 168
MslI CAynn'nnrTG
Cuts at: 1233 2401 2528 1233
Size: 1168 127 3117
Fragments arranged by size:
3117 1168 127
MspA1I CmG'CkG
Cuts at: 135 1523 1837 2197 3210 3455 135
Size: 1388 314 360 1013 245 1092
Fragments arranged by size:
1388 1092 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NspI r_CATG'y
Cuts at: 1465 1965 2872 1465
Size: 500 907 3005
Fragments arranged by size:
3005 907 500
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 429 429
Size: 4412
PspXI vC'TCGA_Gb
Cuts at: 1040 1040
Size: 4412
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 368 400 368
Size: 32 4380
PstI C_TGCA'G
Cuts at: 354 1459 2144 354
Size: 1105 685 2622
Fragments arranged by size:
2622 1105 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 1837 2197 1837
Size: 360 4052
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 180 293 1426 180
Size: 113 1133 3166
Fragments arranged by size:
3166 1133 113
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SfcI C'TryA_G
Cuts at: 33 350 1455 2140 3133 3324 33
Size: 317 1105 685 993 191 1121
Fragments arranged by size:
1121 1105 993 685 317 191
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1434 1434
Size: 4412
SmlI C'TyrA_G
Cuts at: 274 1040 1567 2482 2974 3236 274
Size: 766 527 915 492 262 1450
Fragments arranged by size:
1450 915 766 527 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 1465 1965 1465
Size: 500 3912
SspI AAT'ATT
Cuts at: 1050 1050
Size: 4412
StyI C'CwwG_G
Cuts at: 717 734 1546 1900 717
Size: 17 812 354 3229
Fragments arranged by size:
3229 812 354 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 400 843 3927 400
Size: 443 3084 885
Fragments arranged by size:
3084 885 443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3929 3961 3929
Size: 32 4380
Tth111I GACn'n_nGTC
Cuts at: 1235 2209 1235
Size: 974 3438
XbaI T'CTAG_A
Cuts at: 1149 1443 1149
Size: 294 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 177 335 471 600 1907 177
Size: 158 136 129 1307 2682
Fragments arranged by size:
2682 1307 158 136 129
XhoI C'TCGA_G
Cuts at: 1040 1040
Size: 4412
XmaI C'CCGG_G
Cuts at: 1432 1432
Size: 4412
XmnI GAAnn'nnTTC
Cuts at: 1330 1330
Size: 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PciI PmeI PpiI PpuMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I
BtgI ClaI CspCI EcoNI FseI FspAI HindIII HpaI
MfeI NcoI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
ZraI