Restriction map for
pF1KB6620
(Circular) (Six-base) MAPSORT of: pF1KB6620.seq Check: 6490 from: 1 to: 4418
pF1KB6620 4418 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1456 1456
Size: 4418
Acc65I G'GTAC_C
Cuts at: 1138 1434 1138
Size: 296 4122
AclI AA'CG_TT
Cuts at: 4258 4258
Size: 4418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 2243 2675 3422 3690 584
Size: 1659 432 747 268 1312
Fragments arranged by size:
1659 1312 747 432 268
AflIII A'CryG_T
Cuts at: 352 1770 2874 3851 352
Size: 1418 1104 977 919
Fragments arranged by size:
1418 1104 977 919
AgeI A'CCGG_T
Cuts at: 2806 2806
Size: 4418
AleI CACnn'nnGTG
Cuts at: 1361 1361
Size: 4418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1379 1379
Size: 4418
AlwNI CAG_nnn'CTG
Cuts at: 715 987 1723 3290 715
Size: 272 736 1567 1843
Fragments arranged by size:
1843 1567 736 272
ApaI G_GGCC'C
Cuts at: 516 1387 516
Size: 871 3547
ApaLI G'TGCA_C
Cuts at: 3188 3188
Size: 4418
ApoI r'AATT_y
Cuts at: 363 1422 3771 4027 363
Size: 1059 2349 256 754
Fragments arranged by size:
2349 1059 754 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4418
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
AvaI C'yCGr_G
Cuts at: 436 517 1111 1297 1438 436
Size: 81 594 186 141 3416
Fragments arranged by size:
3416 594 186 141 81
BamHI G'GATC_C
Cuts at: 1443 1443
Size: 4418
BanI G'GyrC_C
Cuts at: 204 301 1059 1138 1337 1363 1434 1848 2096
Size: 97 758 79 199 26 71 414 248
Cuts at: 2096 2131 204
Size: 35 2491
Fragments arranged by size:
2491 758 414 248 199 97 79 71
35 26
BanII G_rGCy'C
Cuts at: 179 266 516 575 1031 1304 1387 1432 2462
Size: 87 250 59 456 273 83 45 1030
Cuts at: 2462 179
Size: 2135
Fragments arranged by size:
2135 1030 456 273 250 87 83 59
45
BbeI G_GCGC'C
Cuts at: 1852 2100 1852
Size: 248 4170
BbsI GAAGACnn'nnnn_
Cuts at: 475 828 475
Size: 353 4065
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 427 461 90
Size: 34 303 34 4047
Fragments arranged by size:
4047 303 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1029 2316 3077 4418 1029
Size: 1287 761 1341 1029
Fragments arranged by size:
1341 1287 1029 761
BclI T'GATC_A
Cuts at: 1938 1938
Size: 4418
BglI GCCn_nnn'nGGC
Cuts at: 299 557 299
Size: 258 4160
BglII A'GATC_T
Cuts at: 1933 3970 1933
Size: 2037 2381
BlpI GC'TnA_GC
Cuts at: 1530 1530
Size: 4418
Bme1580I G_kGCm'C
Cuts at: 516 629 1387 2043 2136 3192 516
Size: 113 758 656 93 1056 1742
Fragments arranged by size:
1742 1056 758 656 113 93
BmrI ACTGGGnnnn_n'
Cuts at: 418 468 714 889 1009 1034 1036 1222 1630
Size: 50 246 175 120 25 2 186 408
Cuts at: 1630 1711 2046 4190 418
Size: 81 335 2144 646
Fragments arranged by size:
2144 646 408 335 246 186 175 120
81 50 25 2
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1022 1253 692
Size: 330 231 3857
Fragments arranged by size:
3857 330 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 877 1594 2473 2965 3263 877
Size: 717 879 492 298 2032
Fragments arranged by size:
2032 879 717 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1229 4122 1229
Size: 2893 1525
BsaAI yAC'GTr
Cuts at: 1771 1771
Size: 4418
BsaBI GATnn'nnATC
Cuts at: 6 1932 3969 6
Size: 1926 2037 455
Fragments arranged by size:
2037 1926 455
BsaHI Gr'CG_yC
Cuts at: 900 1849 2097 2799 4295 900
Size: 949 248 702 1496 1023
Fragments arranged by size:
1496 1023 949 702 248
BsaWI w'CCGG_w
Cuts at: 1607 2128 2806 3080 3227 3638 1607
Size: 521 678 274 147 411 2387
Fragments arranged by size:
2387 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1087 1117 1087
Size: 30 4388
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 454 633 454
Size: 179 4239
BseYI C'CCAG_C
Cuts at: 162 266 649 938 1242 1843 2601 3178 3674
Size: 104 383 289 304 601 758 577 496
Cuts at: 3674 162
Size: 906
Fragments arranged by size:
906 758 601 577 496 383 304 289
104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 438
Size: 4418
BsiEI CG_ry'CG
Cuts at: 104 1960 2006 3214 104
Size: 1856 46 1208 1308
Fragments arranged by size:
1856 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 380 575 1358 1432 2210 2400 3192 380
Size: 195 783 74 778 190 792 1606
Fragments arranged by size:
1606 792 783 778 195 190 74
BsmI GAATG_Cn'
Cuts at: 1148 1148
Size: 4418
Bsp1286I G_dGCh'C
Cuts at: 179 266 380 516 575 629 1031 1304 1358
Size: 87 114 136 59 54 402 273 54
Cuts at: 1358 1387 1432 2043 2136 2210 2400 2462 3192
Size: 29 45 611 93 74 190 62 730
Cuts at: 3192 179
Size: 1405
Fragments arranged by size:
1405 730 611 402 273 190 136 114
93 87 74 62 59 54 54 45
29
BspEI T'CCGG_A
Cuts at: 3638 3638
Size: 4418
BspHI T'CATG_A
Cuts at: 3594 3594
Size: 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 241 742 1468 1682 1984 2365 241
Size: 501 726 214 302 381 2294
Fragments arranged by size:
2294 726 501 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2710 2764 4248 4413 50
Size: 2660 54 1484 165 55
Fragments arranged by size:
2660 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2330 2330
Size: 4418
BsrFI r'CCGG_y
Cuts at: 1159 2416 2597 2806 1159
Size: 1257 181 209 2771
Fragments arranged by size:
2771 1257 209 181
BsrGI T'GTAC_A
Cuts at: 523 523
Size: 4418
BssSI C'ACGA_G
Cuts at: 2689 3047 2689
Size: 358 4060
BstAPI GCAn_nnn'nTGC
Cuts at: 281 1795 281
Size: 1514 2904
BstBI TT'CG_AA
Cuts at: 2779 2779
Size: 4418
BstXI CCAn_nnnn'nTGG
Cuts at: 1324 1324
Size: 4418
BstYI r'GATC_y
Cuts at: 1 1443 1910 1933 2268 2514 3526 3612 3624
Size: 1442 467 23 335 246 1012 86 12
Cuts at: 3624 3970 1
Size: 346 449
Fragments arranged by size:
1442 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 1134 1134
Size: 4418
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 173 2368 2549 2712 173
Size: 2195 181 163 1879
Fragments arranged by size:
2195 1879 181 163
BtsI GCAGTG_nn'
Cuts at: 528 603 713 754 985 1661 528
Size: 75 110 41 231 676 3285
Fragments arranged by size:
3285 676 231 110 75 41
DraI TTT'AAA
Cuts at: 1417 1417
Size: 4418
DraIII CAC_nnn'GTG
Cuts at: 677 677
Size: 4418
DrdI GACnn_nn'nnGTC
Cuts at: 1226 2124 2982 1226
Size: 898 858 2662
Fragments arranged by size:
2662 898 858
EaeI y'GGCC_r
Cuts at: 791 2003 2177 2568 2595 3940 791
Size: 1212 174 391 27 1345 1269
Fragments arranged by size:
1345 1269 1212 391 174 27
EagI C'GGCC_G
Cuts at: 2003 2003
Size: 4418
EarI CTCTTCn'nnn_
Cuts at: 175 847 1271 2441 2651 175
Size: 672 424 1170 210 1942
Fragments arranged by size:
1942 1170 672 424 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2626 2936 3082 4228 2626
Size: 310 146 1146 2816
Fragments arranged by size:
2816 1146 310 146
EcoICRI GAG'CTC
Cuts at: 573 1430 573
Size: 857 3561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 692 1022 1253 2243 2675 3422 3690 584
Size: 108 330 231 990 432 747 268 1312
Fragments arranged by size:
1312 990 747 432 330 268 231 108
EcoNI CCTnn'n_nnAGG
Cuts at: 1377 1377
Size: 4418
EcoO109I rG'GnC_Cy
Cuts at: 151 513 916 1228 1284 1383 1557 151
Size: 362 403 312 56 99 174 3012
Fragments arranged by size:
3012 403 362 312 174 99 56
EcoRI G'AATT_C
Cuts at: 363 1422 363
Size: 1059 3359
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2437 2437
Size: 4418
FspI TGC'GCA
Cuts at: 2199 2199
Size: 4418
HaeII r_GCGC'y
Cuts at: 656 1852 2100 3122 4101 656
Size: 1196 248 1022 979 973
Fragments arranged by size:
1196 1022 979 973 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 875 907 2260 2290 2292 2322 875
Size: 32 1353 30 2 30 2971
Fragments arranged by size:
2971 1353 32 30 30 2
HincII GTy'rAC
Cuts at: 1457 1457
Size: 4418
KasI G'GCGC_C
Cuts at: 1848 2096 1848
Size: 248 4170
KpnI G_GTAC'C
Cuts at: 1142 1438 1142
Size: 296 4122
MluI A'CGCG_T
Cuts at: 3851 3851
Size: 4418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 200 749 1085 3089 3273 200
Size: 549 336 2004 184 1345
Fragments arranged by size:
2004 1345 549 336 184
MscI TGG'CCA
Cuts at: 2179 2179
Size: 4418
MslI CAynn'nnrTG
Cuts at: 784 1361 2407 2534 784
Size: 577 1046 127 2668
Fragments arranged by size:
2668 1046 577 127
MspA1I CmG'CkG
Cuts at: 290 405 482 659 1529 1843 2203 3216 3461
Size: 115 77 177 870 314 360 1013 245
Cuts at: 3461 290
Size: 1247
Fragments arranged by size:
1247 1013 870 360 314 245 177 115
77
NaeI GCC'GGC
Cuts at: 2599 2599
Size: 4418
NarI GG'CG_CC
Cuts at: 1849 2097 1849
Size: 248 4170
NdeI CA'TA_TG
Cuts at: 213 213
Size: 4418
NgoMIV G'CCGG_C
Cuts at: 2597 2597
Size: 4418
NspI r_CATG'y
Cuts at: 356 1471 1971 2878 356
Size: 1115 500 907 1896
Fragments arranged by size:
1896 1115 907 500
PciI A'CATG_T
Cuts at: 352 2874 352
Size: 2522 1896
PflMI CCAn_nnn'nTGG
Cuts at: 693 693
Size: 4418
PfoI T'CCnGG_A
Cuts at: 332 1230 332
Size: 898 3520
PmeI GTTT'AAAC
Cuts at: 1417 1417
Size: 4418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 3557
Size: 32 4386
PpuMI rG'GwC_Cy
Cuts at: 916 1228 1284 916
Size: 312 56 4050
Fragments arranged by size:
4050 312 56
PspOMI G'GGCC_C
Cuts at: 512 1383 512
Size: 871 3547
PspXI vC'TCGA_Gb
Cuts at: 436 517 436
Size: 81 4337
PstI C_TGCA'G
Cuts at: 238 874 1465 2150 238
Size: 636 591 685 2506
Fragments arranged by size:
2506 685 636 591
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4418
PvuII CAG'CTG
Cuts at: 405 1843 2203 405
Size: 1438 360 2620
Fragments arranged by size:
2620 1438 360
RsrII CG'GwC_CG
Cuts at: 2613 2613
Size: 4418
SacI G_AGCT'C
Cuts at: 575 1432 575
Size: 857 3561
SalI G'TCGA_C
Cuts at: 1455 1455
Size: 4418
SanDI GG'GwC_CC
Cuts at: 916 1284 916
Size: 368 4050
SapI GCTCTTCn'nnn_
Cuts at: 847 2441 2651 847
Size: 1594 210 2614
Fragments arranged by size:
2614 1594 210
SbfI CC_TGCA'GG
Cuts at: 238 1465 238
Size: 1227 3191
ScaI AGT'ACT
Cuts at: 1211 3935 1211
Size: 2724 1694
SexAI A'CCwGG_T
Cuts at: 673 673
Size: 4418
SfcI C'TryA_G
Cuts at: 33 234 870 926 1461 2146 3139 3330 33
Size: 201 636 56 535 685 993 191 1121
Fragments arranged by size:
1121 993 685 636 535 201 191 56
SfoI GGC'GCC
Cuts at: 1850 2098 1850
Size: 248 4170
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4418
SgrAI Cr'CCGG_yG
Cuts at: 1159 1159
Size: 4418
SmaI CCC'GGG
Cuts at: 1113 1299 1440 1113
Size: 186 141 4091
Fragments arranged by size:
4091 186 141
SmlI C'TyrA_G
Cuts at: 436 517 892 1573 2488 2980 3242 436
Size: 81 375 681 915 492 262 1612
Fragments arranged by size:
1612 915 681 492 375 262 81
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4418
SphI G_CATG'C
Cuts at: 1471 1971 1471
Size: 500 3918
StyI C'CwwG_G
Cuts at: 118 687 1387 1552 1906 118
Size: 569 700 165 354 2630
Fragments arranged by size:
2630 700 569 354 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1420 2001 2134 2593 2802 1420
Size: 581 133 459 209 3036
Fragments arranged by size:
3036 581 459 209 133
TatI w'GTAC_w
Cuts at: 523 1209 3933 523
Size: 686 2724 1008
Fragments arranged by size:
2724 1008 686
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3935 3967 3935
Size: 32 4386
Tth111I GACn'n_nGTC
Cuts at: 2215 2215
Size: 4418
XbaI T'CTAG_A
Cuts at: 1449 1449
Size: 4418
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 786 1141 1321 1913 786
Size: 355 180 592 3291
Fragments arranged by size:
3291 592 355 180
XhoI C'TCGA_G
Cuts at: 436 517 436
Size: 81 4337
XmaI C'CCGG_G
Cuts at: 1111 1297 1438 1111
Size: 186 141 4091
Fragments arranged by size:
4091 186 141
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NheI NotI NruI
NsiI PacI PasI PmlI PshAI PsiI PsrI SacII
SfiI SnaBI SrfI SspI StuI SwaI XmnI ZraI