Restriction map for
pF1KB6624
(Circular) (Six-base) MAPSORT of: pF1KB6624.seq Check: 4859 from: 1 to: 4412
pF1KB6624 4412 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 520 520
Size: 4412
AccI GT'mk_AC
Cuts at: 476 1400 1450 476
Size: 924 50 3438
Fragments arranged by size:
3438 924 50
Acc65I G'GTAC_C
Cuts at: 1428 1428
Size: 4412
AclI AA'CG_TT
Cuts at: 4252 4252
Size: 4412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 2237 2669 3416 3684 641
Size: 1596 432 747 268 1369
Fragments arranged by size:
1596 1369 747 432 268
AflIII A'CryG_T
Cuts at: 1764 2868 3845 1764
Size: 1104 977 2331
Fragments arranged by size:
2331 1104 977
AgeI A'CCGG_T
Cuts at: 2800 2800
Size: 4412
AlwNI CAG_nnn'CTG
Cuts at: 1717 3284 1717
Size: 1567 2845
ApaLI G'TGCA_C
Cuts at: 3182 3182
Size: 4412
ApoI r'AATT_y
Cuts at: 286 1416 3765 4021 286
Size: 1130 2349 256 677
Fragments arranged by size:
2349 1130 677 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4412
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
AvaI C'yCGr_G
Cuts at: 1432 1432
Size: 4412
AvrII C'CTAG_G
Cuts at: 1098 1098
Size: 4412
BamHI G'GATC_C
Cuts at: 360 1210 1437 360
Size: 850 227 3335
Fragments arranged by size:
3335 850 227
BanI G'GyrC_C
Cuts at: 1428 1842 2090 2125 1428
Size: 414 248 35 3715
Fragments arranged by size:
3715 414 248 35
BanII G_rGCy'C
Cuts at: 917 1061 1426 2456 917
Size: 144 365 1030 2873
Fragments arranged by size:
2873 1030 365 144
BbeI G_GCGC'C
Cuts at: 1846 2094 1846
Size: 248 4164
BbsI GAAGACnn'nnnn_
Cuts at: 790 1319 790
Size: 529 3883
BbvCI CC'TCA_GC
Cuts at: 799 799
Size: 4412
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4378
BciVI GTATCCnnnnn_n'
Cuts at: 2310 3071 4412 2310
Size: 761 1341 2310
Fragments arranged by size:
2310 1341 761
BclI T'GATC_A
Cuts at: 1932 1932
Size: 4412
BfrBI ATG'CAT
Cuts at: 401 401
Size: 4412
BglII A'GATC_T
Cuts at: 620 1253 1927 3964 620
Size: 633 674 2037 1068
Fragments arranged by size:
2037 1068 674 633
BlpI GC'TnA_GC
Cuts at: 1060 1524 1060
Size: 464 3948
Bme1580I G_kGCm'C
Cuts at: 526 2037 2130 3186 526
Size: 1511 93 1056 1752
Fragments arranged by size:
1752 1511 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 520 1624 1705 2040 4184 520
Size: 1104 81 335 2144 748
Fragments arranged by size:
2144 1104 748 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2779 2779
Size: 4412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 730
Size: 4412
Bpu10I CC'TnA_GC
Cuts at: 356 799 356
Size: 443 3969
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 97 392 1588 2467 2959 3257 97
Size: 295 1196 879 492 298 1252
Fragments arranged by size:
1252 1196 879 492 298 295
BsaI GGTCTCn'nnnn_
Cuts at: 4116 4116
Size: 4412
BsaAI yAC'GTr
Cuts at: 1765 1765
Size: 4412
BsaBI GATnn'nnATC
Cuts at: 6 1926 3963 6
Size: 1920 2037 455
Fragments arranged by size:
2037 1920 455
BsaHI Gr'CG_yC
Cuts at: 1843 2091 2793 4289 1843
Size: 248 702 1496 1966
Fragments arranged by size:
1966 1496 702 248
BsaWI w'CCGG_w
Cuts at: 440 1258 1601 2122 2800 3074 3221 3632 440
Size: 818 343 521 678 274 147 411 1220
Fragments arranged by size:
1220 818 678 521 411 343 274 147
BseYI C'CCAG_C
Cuts at: 538 1837 2595 3172 3668 538
Size: 1299 758 577 496 1282
Fragments arranged by size:
1299 1282 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 174 161
Size: 13 4399
BsiEI CG_ry'CG
Cuts at: 104 205 1954 2000 3208 104
Size: 101 1749 46 1208 1308
Fragments arranged by size:
1749 1308 1208 101 46
BsiHKAI G_wGCw'C
Cuts at: 917 1061 1426 2204 2394 3186 917
Size: 144 365 778 190 792 2143
Fragments arranged by size:
2143 792 778 365 190 144
BsmI GAATG_Cn'
Cuts at: 958 958
Size: 4412
BsmBI CGTCTCn'nnnn_
Cuts at: 287 287
Size: 4412
Bsp1286I G_dGCh'C
Cuts at: 526 917 1061 1426 2037 2130 2204 2394 2456
Size: 391 144 365 611 93 74 190 62
Cuts at: 2456 3186 526
Size: 730 1752
Fragments arranged by size:
1752 730 611 391 365 190 144 93
74 62
BspEI T'CCGG_A
Cuts at: 1258 3632 1258
Size: 2374 2038
BspHI T'CATG_A
Cuts at: 759 1284 3588 759
Size: 525 2304 1583
Fragments arranged by size:
2304 1583 525
BspMI ACCTGCnnnn'nnnn_
Cuts at: 520 902 1462 1676 1978 2359 520
Size: 382 560 214 302 381 2573
Fragments arranged by size:
2573 560 382 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2704 2758 4242 4407 50
Size: 2654 54 1484 165 55
Fragments arranged by size:
2654 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 401 642 793 2324 401
Size: 241 151 1531 2489
Fragments arranged by size:
2489 1531 241 151
BsrFI r'CCGG_y
Cuts at: 2410 2591 2800 2410
Size: 181 209 4022
Fragments arranged by size:
4022 209 181
BssSI C'ACGA_G
Cuts at: 2683 3041 2683
Size: 358 4054
BstAPI GCAn_nnn'nTGC
Cuts at: 521 1237 1789 521
Size: 716 552 3144
Fragments arranged by size:
3144 716 552
BstBI TT'CG_AA
Cuts at: 2773 2773
Size: 4412
BstYI r'GATC_y
Cuts at: 1 348 360 620 1210 1253 1437 1904 1927
Size: 347 12 260 590 43 184 467 23
Cuts at: 1927 2262 2508 3520 3606 3618 3964 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 590 467 449 347 346 335 260
246 184 86 43 23 12 12
BstZ17I GTA'TAC
Cuts at: 1401 1401
Size: 4412
BtgI C'CryG_G
Cuts at: 176 176
Size: 4412
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2362 2543 2706 2362
Size: 181 163 4068
Fragments arranged by size:
4068 181 163
BtsI GCAGTG_nn'
Cuts at: 493 526 708 1167 1655 493
Size: 33 182 459 488 3250
Fragments arranged by size:
3250 488 459 182 33
DraI TTT'AAA
Cuts at: 1411 1411
Size: 4412
DrdI GACnn_nn'nnGTC
Cuts at: 268 2118 2976 268
Size: 1850 858 1704
Fragments arranged by size:
1850 1704 858
EaeI y'GGCC_r
Cuts at: 1997 2171 2562 2589 3934 1997
Size: 174 391 27 1345 2475
Fragments arranged by size:
2475 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1997 1997
Size: 4412
EarI CTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2620 2930 3076 4222 2620
Size: 310 146 1146 2810
Fragments arranged by size:
2810 1146 310 146
EcoICRI GAG'CTC
Cuts at: 915 1059 1424 915
Size: 144 365 3903
Fragments arranged by size:
3903 365 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 641 730 2237 2669 3416 3684 641
Size: 89 1507 432 747 268 1369
Fragments arranged by size:
1507 1369 747 432 268 89
EcoO109I rG'GnC_Cy
Cuts at: 496 753 1551 496
Size: 257 798 3357
Fragments arranged by size:
3357 798 257
EcoRI G'AATT_C
Cuts at: 1416 1416
Size: 4412
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 978 2431 978
Size: 1453 2959
FspI TGC'GCA
Cuts at: 2193 2193
Size: 4412
HaeII r_GCGC'y
Cuts at: 1846 2094 3116 4095 1846
Size: 248 1022 979 2163
Fragments arranged by size:
2163 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1094 1126 2254 2284 2286 2316 1094
Size: 32 1128 30 2 30 3190
Fragments arranged by size:
3190 1128 32 30 30 2
HincII GTy'rAC
Cuts at: 1185 1451 1185
Size: 266 4146
KasI G'GCGC_C
Cuts at: 1842 2090 1842
Size: 248 4164
KpnI G_GTAC'C
Cuts at: 1432 1432
Size: 4412
MluI A'CGCG_T
Cuts at: 3845 3845
Size: 4412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 185 252 1146 3083 3267 185
Size: 67 894 1937 184 1330
Fragments arranged by size:
1937 1330 894 184 67
MscI TGG'CCA
Cuts at: 2173 2173
Size: 4412
MslI CAynn'nnrTG
Cuts at: 764 1160 2401 2528 764
Size: 396 1241 127 2648
Fragments arranged by size:
2648 1241 396 127
MspA1I CmG'CkG
Cuts at: 172 178 1523 1837 2197 3210 3455 172
Size: 6 1345 314 360 1013 245 1129
Fragments arranged by size:
1345 1129 1013 360 314 245 6
NaeI GCC'GGC
Cuts at: 2593 2593
Size: 4412
NarI GG'CG_CC
Cuts at: 1843 2091 1843
Size: 248 4164
NgoMIV G'CCGG_C
Cuts at: 2591 2591
Size: 4412
NsiI A_TGCA'T
Cuts at: 403 403
Size: 4412
NspI r_CATG'y
Cuts at: 605 701 1465 1965 2872 605
Size: 96 764 500 907 2145
Fragments arranged by size:
2145 907 764 500 96
PciI A'CATG_T
Cuts at: 2868 2868
Size: 4412
PfoI T'CCnGG_A
Cuts at: 321 321
Size: 4412
PmeI GTTT'AAAC
Cuts at: 1411 1411
Size: 4412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3551 3583 3551
Size: 32 4380
PpuMI rG'GwC_Cy
Cuts at: 496 496
Size: 4412
PshAI GACnn'nnGTC
Cuts at: 424 424
Size: 4412
PstI C_TGCA'G
Cuts at: 192 426 1459 2144 192
Size: 234 1033 685 2460
Fragments arranged by size:
2460 1033 685 234
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4412
PvuII CAG'CTG
Cuts at: 172 1837 2197 172
Size: 1665 360 2387
Fragments arranged by size:
2387 1665 360
RsrII CG'GwC_CG
Cuts at: 2607 2607
Size: 4412
SacI G_AGCT'C
Cuts at: 917 1061 1426 917
Size: 144 365 3903
Fragments arranged by size:
3903 365 144
SacII CC_GC'GG
Cuts at: 179 179
Size: 4412
SalI G'TCGA_C
Cuts at: 1449 1449
Size: 4412
SanDI GG'GwC_CC
Cuts at: 496 496
Size: 4412
SapI GCTCTTCn'nnn_
Cuts at: 2435 2645 2435
Size: 210 4202
SbfI CC_TGCA'GG
Cuts at: 1459 1459
Size: 4412
ScaI AGT'ACT
Cuts at: 3929 3929
Size: 4412
SfcI C'TryA_G
Cuts at: 33 188 422 1241 1455 2140 3133 3324 33
Size: 155 234 819 214 685 993 191 1121
Fragments arranged by size:
1121 993 819 685 234 214 191 155
SfoI GGC'GCC
Cuts at: 1844 2092 1844
Size: 248 4164
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4412
SmaI CCC'GGG
Cuts at: 1434 1434
Size: 4412
SmlI C'TyrA_G
Cuts at: 112 407 1567 2482 2974 3236 112
Size: 295 1160 915 492 262 1288
Fragments arranged by size:
1288 1160 915 492 295 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4412
SphI G_CATG'C
Cuts at: 701 1465 1965 701
Size: 764 500 3148
Fragments arranged by size:
3148 764 500
SspI AAT'ATT
Cuts at: 1068 1068
Size: 4412
StuI AGG'CCT
Cuts at: 804 804
Size: 4412
StyI C'CwwG_G
Cuts at: 953 1098 1546 1900 953
Size: 145 448 354 3465
Fragments arranged by size:
3465 448 354 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1995 2128 2587 2796 1995
Size: 133 459 209 3611
Fragments arranged by size:
3611 459 209 133
TatI w'GTAC_w
Cuts at: 673 3927 673
Size: 3254 1158
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 303 335 3929 3961 303
Size: 32 3594 32 754
Fragments arranged by size:
3594 754 32 32
Tth111I GACn'n_nGTC
Cuts at: 2209 2209
Size: 4412
XbaI T'CTAG_A
Cuts at: 1443 1443
Size: 4412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1907 1907
Size: 4412
XmaI C'CCGG_G
Cuts at: 1432 1432
Size: 4412
XmnI GAAnn'nnTTC
Cuts at: 286 286
Size: 4412
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BglI BmgBI BmtI BsaXI BseRI BsiWI
BsrGI BssHII BstEII BstXI Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PflMI PmlI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SwaI XhoI ZraI