Restriction map for
pF1KB6625
(Circular) (Six-base) MAPSORT of: pF1KB6625.seq Check: 4893 from: 1 to: 4331
pF1KB6625 4331 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 504 504
Size: 4331
AatII G_ACGT'C
Cuts at: 977 977
Size: 4331
AccI GT'mk_AC
Cuts at: 1369 1369
Size: 4331
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 167 4171 167
Size: 4004 327
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2156 2588 3335 3603 2156
Size: 432 747 268 2884
Fragments arranged by size:
2884 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 1636 3203 1636
Size: 1567 2764
ApaI G_GGCC'C
Cuts at: 642 1009 642
Size: 367 3964
ApaLI G'TGCA_C
Cuts at: 544 3101 544
Size: 2557 1774
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 243 732 1137 1351 243
Size: 489 405 214 3223
Fragments arranged by size:
3223 489 405 214
BamHI G'GATC_C
Cuts at: 805 1356 805
Size: 551 3780
BanI G'GyrC_C
Cuts at: 1264 1347 1761 2009 2044 1264
Size: 83 414 248 35 3551
Fragments arranged by size:
3551 414 248 83 35
BanII G_rGCy'C
Cuts at: 642 1009 1118 1189 1213 1345 2375 642
Size: 367 109 71 24 132 1030 2598
Fragments arranged by size:
2598 1030 367 132 109 71 24
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 608 608
Size: 4331
BbvCI CC'TCA_GC
Cuts at: 274 518 274
Size: 244 4087
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 1112 1443 1112
Size: 331 4000
Bme1580I G_kGCm'C
Cuts at: 548 642 1009 1267 1295 1956 2049 3105 548
Size: 94 367 258 28 661 93 1056 1774
Fragments arranged by size:
1774 1056 661 367 258 94 93 28
BmgBI CAC'GTC
Cuts at: 1157 1157
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 1332 1543 1624 1959 4103 1332
Size: 211 81 335 2144 1560
Fragments arranged by size:
2144 1560 335 211 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 388 505 759 776 897 256
Size: 132 117 254 17 121 3690
Fragments arranged by size:
3690 254 132 121 117 17
Bpu10I CC'TnA_GC
Cuts at: 274 518 274
Size: 244 4087
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 343 847 1507 2386 2878 3176 343
Size: 504 660 879 492 298 1498
Fragments arranged by size:
1498 879 660 504 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 485 927 1090 4035 485
Size: 442 163 2945 781
Fragments arranged by size:
2945 781 442 163
BsaAI yAC'GTr
Cuts at: 657 1684 657
Size: 1027 3304
BsaBI GATnn'nnATC
Cuts at: 6 386 1845 3882 6
Size: 380 1459 2037 455
Fragments arranged by size:
2037 1459 455 380
BsaHI Gr'CG_yC
Cuts at: 826 934 974 1762 2010 2712 4208 826
Size: 108 40 788 248 702 1496 949
Fragments arranged by size:
1496 949 788 702 248 108 40
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 610 640 610
Size: 30 4301
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 573 1194 1197 573
Size: 621 3 3707
Fragments arranged by size:
3707 621 3
BseYI C'CCAG_C
Cuts at: 834 1196 1307 1756 2514 3091 3587 834
Size: 362 111 449 758 577 496 1578
Fragments arranged by size:
1578 758 577 496 449 362 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 172
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 1289 1873 1919 3127 104
Size: 1185 584 46 1208 1308
Fragments arranged by size:
1308 1208 1185 584 46
BsiHKAI G_wGCw'C
Cuts at: 244 548 1189 1345 2123 2313 3105 244
Size: 304 641 156 778 190 792 1470
Fragments arranged by size:
1470 792 778 641 304 190 156
BsmI GAATG_Cn'
Cuts at: 351 351
Size: 4331
Bsp1286I G_dGCh'C
Cuts at: 244 548 642 1009 1118 1189 1213 1267 1295
Size: 304 94 367 109 71 24 54 28
Cuts at: 1295 1345 1956 2049 2123 2313 2375 3105 244
Size: 50 611 93 74 190 62 730 1470
Fragments arranged by size:
1470 730 611 367 304 190 109 94
93 74 71 62 54 50 28 24
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 504 1381 1595 1897 2278 504
Size: 877 214 302 381 2557
Fragments arranged by size:
2557 877 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 764 1284 2329 2510 2719 764
Size: 520 1045 181 209 2376
Fragments arranged by size:
2376 1045 520 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 1708 1708
Size: 4331
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstYI r'GATC_y
Cuts at: 1 227 805 1356 1823 1846 2181 2427 3439
Size: 226 578 551 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 578 551 467 449 346 335 246
226 86 23 12
BtgI C'CryG_G
Cuts at: 107 255 428 539 107
Size: 148 173 111 3899
Fragments arranged by size:
3899 173 148 111
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 1283 1574 1283
Size: 291 4040
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 534 1131 2037 2895 534
Size: 597 906 858 1970
Fragments arranged by size:
1970 906 858 597
EaeI y'GGCC_r
Cuts at: 110 461 558 1286 1916 2090 2481 2508 3853
Size: 351 97 728 630 174 391 27 1345
Cuts at: 3853 110
Size: 588
Fragments arranged by size:
1345 728 630 588 391 351 174 97
27
EagI C'GGCC_G
Cuts at: 1286 1916 1286
Size: 630 3701
EarI CTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1187 1343 1187
Size: 156 4175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 388 505 759 776 897 2156 2588 3335
Size: 132 117 254 17 121 1259 432 747
Cuts at: 3335 3603 256
Size: 268 984
Fragments arranged by size:
1259 984 747 432 268 254 132 121
117 17
EcoO109I rG'GnC_Cy
Cuts at: 638 1005 1006 1470 638
Size: 367 1 464 3499
Fragments arranged by size:
3499 464 367 1
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 872 2112 872
Size: 1240 3091
HaeII r_GCGC'y
Cuts at: 794 1765 2013 3035 4014 794
Size: 971 248 1022 979 1111
Fragments arranged by size:
1111 1022 979 971 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 501 533 610 642 895 927 1006 1038 2173
Size: 32 77 32 253 32 79 32 1135
Cuts at: 2173 2203 2205 2235 501
Size: 30 2 30 2597
Fragments arranged by size:
2597 1135 253 79 77 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1370 1370
Size: 4331
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3002 3186 3002
Size: 184 4147
MscI TGG'CCA
Cuts at: 112 560 2092 112
Size: 448 1532 2351
Fragments arranged by size:
2351 1532 448
MslI CAynn'nnrTG
Cuts at: 2320 2447 2320
Size: 127 4204
MspA1I CmG'CkG
Cuts at: 257 430 1049 1151 1196 1442 1756 2116 3129
Size: 173 619 102 45 246 314 360 1013
Cuts at: 3129 3374 257
Size: 245 1214
Fragments arranged by size:
1214 1013 619 360 314 246 245 173
102 45
NaeI GCC'GGC
Cuts at: 1286 2512 1286
Size: 1226 3105
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 539 107
Size: 432 3899
NgoMIV G'CCGG_C
Cuts at: 1284 2510 1284
Size: 1226 3105
NspI r_CATG'y
Cuts at: 1384 1884 2791 1384
Size: 500 907 2924
Fragments arranged by size:
2924 907 500
PasI CC'CwG_GG
Cuts at: 642 642
Size: 4331
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PfoI T'CCnGG_A
Cuts at: 365 365
Size: 4331
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PspOMI G'GGCC_C
Cuts at: 638 1005 638
Size: 367 3964
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1151 1196 1756 2116 1151
Size: 45 560 360 3366
Fragments arranged by size:
3366 560 360 45
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1189 1345 1189
Size: 156 4175
SacII CC_GC'GG
Cuts at: 258 431 258
Size: 173 4158
SalI G'TCGA_C
Cuts at: 1368 1368
Size: 4331
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 1374 2059 3052 3243 33
Size: 1341 685 993 191 1121
Fragments arranged by size:
1341 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 322 862 1486 2401 2893 3155 322
Size: 540 624 915 492 262 1498
Fragments arranged by size:
1498 915 624 540 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
StyI C'CwwG_G
Cuts at: 107 539 782 938 1010 1465 1819 107
Size: 432 243 156 72 455 354 2619
Fragments arranged by size:
2619 455 432 354 243 156 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 413 728 3846 413
Size: 315 3118 898
Fragments arranged by size:
3118 898 315
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
ZraI GAC'GTC
Cuts at: 975 975
Size: 4331
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AleI AlfI AloI AscI AvrII
BaeI BcgI BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PpuMI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI