Restriction map for
pF1KB6626
(Circular) (Six-base) MAPSORT of: pF1KB6626.seq Check: 6998 from: 1 to: 4430
pF1KB6626 4430 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1052 1052
Size: 4430
AccI GT'mk_AC
Cuts at: 1468 1468
Size: 4430
Acc65I G'GTAC_C
Cuts at: 425 1446 425
Size: 1021 3409
AclI AA'CG_TT
Cuts at: 4270 4270
Size: 4430
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 301 451 2255 2687 3434 3702 196
Size: 105 150 1804 432 747 268 924
Fragments arranged by size:
1804 924 747 432 268 150 105
AflIII A'CryG_T
Cuts at: 1782 2886 3863 1782
Size: 1104 977 2349
Fragments arranged by size:
2349 1104 977
AgeI A'CCGG_T
Cuts at: 2818 2818
Size: 4430
AleI CACnn'nnGTG
Cuts at: 970 970
Size: 4430
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1259 1259
Size: 4430
AlwNI CAG_nnn'CTG
Cuts at: 1185 1735 3302 1185
Size: 550 1567 2313
Fragments arranged by size:
2313 1567 550
ApaLI G'TGCA_C
Cuts at: 3200 3200
Size: 4430
ApoI r'AATT_y
Cuts at: 1434 3783 4039 1434
Size: 2349 256 1825
Fragments arranged by size:
2349 1825 256
AseI AT'TA_AT
Cuts at: 20 1346 20
Size: 1326 3104
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
AvaI C'yCGr_G
Cuts at: 1189 1450 1189
Size: 261 4169
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 460 493 460
Size: 33 4397
BamHI G'GATC_C
Cuts at: 111 722 1455 111
Size: 611 733 3086
Fragments arranged by size:
3086 733 611
BanI G'GyrC_C
Cuts at: 314 425 1155 1446 1860 2108 2143 314
Size: 111 730 291 414 248 35 2601
Fragments arranged by size:
2601 730 414 291 248 111 35
BanII G_rGCy'C
Cuts at: 778 881 1091 1424 1444 2474 778
Size: 103 210 333 20 1030 2734
Fragments arranged by size:
2734 1030 333 210 103 20
BbeI G_GCGC'C
Cuts at: 1864 2112 1864
Size: 248 4182
BbvCI CC'TCA_GC
Cuts at: 517 517
Size: 4430
BciVI GTATCCnnnnn_n'
Cuts at: 2328 3089 4430 2328
Size: 761 1341 2328
Fragments arranged by size:
2328 1341 761
BclI T'GATC_A
Cuts at: 1950 1950
Size: 4430
BglI GCCn_nnn'nGGC
Cuts at: 807 807
Size: 4430
BglII A'GATC_T
Cuts at: 662 1945 3982 662
Size: 1283 2037 1110
Fragments arranged by size:
2037 1283 1110
BlpI GC'TnA_GC
Cuts at: 691 1542 691
Size: 851 3579
Bme1580I G_kGCm'C
Cuts at: 2055 2148 3204 2055
Size: 93 1056 3281
Fragments arranged by size:
3281 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1642 1723 2058 4202 1642
Size: 81 335 2144 1870
Fragments arranged by size:
2144 1870 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2797 2797
Size: 4430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 730 838 1162 730
Size: 108 324 3998
Fragments arranged by size:
3998 324 108
Bpu10I CC'TnA_GC
Cuts at: 517 682 1085 517
Size: 165 403 3862
Fragments arranged by size:
3862 403 165
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1045 1321 1606 2485 2977 3275 1045
Size: 276 285 879 492 298 2200
Fragments arranged by size:
2200 879 492 298 285 276
BsaI GGTCTCn'nnnn_
Cuts at: 4134 4134
Size: 4430
BsaAI yAC'GTr
Cuts at: 453 702 1783 453
Size: 249 1081 3100
Fragments arranged by size:
3100 1081 249
BsaBI GATnn'nnATC
Cuts at: 6 1944 3981 6
Size: 1938 2037 455
Fragments arranged by size:
2037 1938 455
BsaHI Gr'CG_yC
Cuts at: 1861 2109 2811 4307 1861
Size: 248 702 1496 1984
Fragments arranged by size:
1984 1496 702 248
BsaWI w'CCGG_w
Cuts at: 950 1619 2140 2818 3092 3239 3650 950
Size: 669 521 678 274 147 411 1730
Fragments arranged by size:
1730 678 669 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 496
Size: 30 4400
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1207 1207
Size: 4430
BseYI C'CCAG_C
Cuts at: 391 717 1414 1855 2613 3190 3686 391
Size: 326 697 441 758 577 496 1135
Fragments arranged by size:
1135 758 697 577 496 441 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1006 1024 1229 1255 466
Size: 540 18 205 26 3641
Fragments arranged by size:
3641 540 205 26 18
BsiEI CG_ry'CG
Cuts at: 104 1972 2018 3226 104
Size: 1868 46 1208 1308
Fragments arranged by size:
1868 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 701 740 1444 2222 2412 3204 701
Size: 39 704 778 190 792 1927
Fragments arranged by size:
1927 792 778 704 190 39
BsmI GAATG_Cn'
Cuts at: 389 389
Size: 4430
Bsp1286I G_dGCh'C
Cuts at: 701 740 778 881 1091 1424 1444 2055 2148
Size: 39 38 103 210 333 20 611 93
Cuts at: 2148 2222 2412 2474 3204 701
Size: 74 190 62 730 1927
Fragments arranged by size:
1927 730 611 333 210 190 103 93
74 62 39 38 20
BspEI T'CCGG_A
Cuts at: 950 3650 950
Size: 2700 1730
BspHI T'CATG_A
Cuts at: 3606 3606
Size: 4430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1052 1161 1313 1480 1694 1996 2377 1052
Size: 109 152 167 214 302 381 3105
Fragments arranged by size:
3105 381 302 214 167 152 109
BsrBI CCG'CTC
Cuts at: 50 117 2722 2776 4260 4425 50
Size: 67 2605 54 1484 165 55
Fragments arranged by size:
2605 1484 165 67 55 54
BsrDI GCAATG_nn'
Cuts at: 2342 2342
Size: 4430
BsrFI r'CCGG_y
Cuts at: 2428 2609 2818 2428
Size: 181 209 4040
Fragments arranged by size:
4040 209 181
BsrGI T'GTAC_A
Cuts at: 581 581
Size: 4430
BssSI C'ACGA_G
Cuts at: 2701 3059 2701
Size: 358 4072
BstAPI GCAn_nnn'nTGC
Cuts at: 1807 1807
Size: 4430
BstBI TT'CG_AA
Cuts at: 2791 2791
Size: 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 832 832
Size: 4430
BstYI r'GATC_y
Cuts at: 1 111 662 722 1455 1922 1945 2280 2526
Size: 110 551 60 733 467 23 335 246
Cuts at: 2526 3538 3624 3636 3982 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 733 551 467 449 346 335 246
110 86 60 23 12
BtgI C'CryG_G
Cuts at: 107 486 805 107
Size: 379 319 3732
Fragments arranged by size:
3732 379 319
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 291 2380 2561 2724 291
Size: 2089 181 163 1997
Fragments arranged by size:
2089 1997 181 163
BtsI GCAGTG_nn'
Cuts at: 1165 1673 1165
Size: 508 3922
ClaI AT'CG_AT
Cuts at: 191 191
Size: 4430
DraI TTT'AAA
Cuts at: 1429 1429
Size: 4430
DraIII CAC_nnn'GTG
Cuts at: 1252 1252
Size: 4430
DrdI GACnn_nn'nnGTC
Cuts at: 2136 2994 2136
Size: 858 3572
EaeI y'GGCC_r
Cuts at: 959 1325 2015 2189 2580 2607 3952 959
Size: 366 690 174 391 27 1345 1437
Fragments arranged by size:
1437 1345 690 391 366 174 27
EagI C'GGCC_G
Cuts at: 2015 2015
Size: 4430
EarI CTCTTCn'nnn_
Cuts at: 631 766 2453 2663 631
Size: 135 1687 210 2398
Fragments arranged by size:
2398 1687 210 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 369 2638 2948 3094 4240 369
Size: 2269 310 146 1146 559
Fragments arranged by size:
2269 1146 559 310 146
EcoICRI GAG'CTC
Cuts at: 1442 1442
Size: 4430
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 301 451 730 838 1162 2255 2687 3434
Size: 105 150 279 108 324 1093 432 747
Cuts at: 3434 3702 196
Size: 268 924
Fragments arranged by size:
1093 924 747 432 324 279 268 150
108 105
EcoO109I rG'GnC_Cy
Cuts at: 1173 1569 1173
Size: 396 4034
EcoRI G'AATT_C
Cuts at: 1434 1434
Size: 4430
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2449 2449
Size: 4430
FspI TGC'GCA
Cuts at: 2211 2211
Size: 4430
HaeII r_GCGC'y
Cuts at: 1864 2112 3134 4113 1864
Size: 248 1022 979 2181
Fragments arranged by size:
2181 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 2272 2302 2304 2334 103
Size: 32 2137 30 2 30 2199
Fragments arranged by size:
2199 2137 32 30 30 2
HincII GTy'rAC
Cuts at: 1469 1469
Size: 4430
KasI G'GCGC_C
Cuts at: 1860 2108 1860
Size: 248 4182
KpnI G_GTAC'C
Cuts at: 429 1450 429
Size: 1021 3409
MluI A'CGCG_T
Cuts at: 3863 3863
Size: 4430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 619 3101 3285 619
Size: 2482 184 1764
Fragments arranged by size:
2482 1764 184
MscI TGG'CCA
Cuts at: 2191 2191
Size: 4430
MslI CAynn'nnrTG
Cuts at: 970 2419 2546 970
Size: 1449 127 2854
Fragments arranged by size:
2854 1449 127
MspA1I CmG'CkG
Cuts at: 174 521 717 1418 1541 1855 2215 3228 3473
Size: 347 196 701 123 314 360 1013 245
Cuts at: 3473 174
Size: 1131
Fragments arranged by size:
1131 1013 701 360 347 314 245 196
123
NaeI GCC'GGC
Cuts at: 2611 2611
Size: 4430
NarI GG'CG_CC
Cuts at: 1861 2109 1861
Size: 248 4182
NcoI C'CATG_G
Cuts at: 107 107
Size: 4430
NgoMIV G'CCGG_C
Cuts at: 2609 2609
Size: 4430
NspI r_CATG'y
Cuts at: 1483 1983 2890 1483
Size: 500 907 3023
Fragments arranged by size:
3023 907 500
PciI A'CATG_T
Cuts at: 2886 2886
Size: 4430
PflMI CCAn_nnn'nTGG
Cuts at: 324 453 1122 324
Size: 129 669 3632
Fragments arranged by size:
3632 669 129
PfoI T'CCnGG_A
Cuts at: 854 977 1073 854
Size: 123 96 4211
Fragments arranged by size:
4211 123 96
PmeI GTTT'AAAC
Cuts at: 1429 1429
Size: 4430
PmlI CAC'GTG
Cuts at: 702 702
Size: 4430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 193 225 3569 3601 193
Size: 32 3344 32 1022
Fragments arranged by size:
3344 1022 32 32
PpuMI rG'GwC_Cy
Cuts at: 1173 1173
Size: 4430
PstI C_TGCA'G
Cuts at: 131 848 1172 1477 2162 131
Size: 717 324 305 685 2399
Fragments arranged by size:
2399 717 685 324 305
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4430
PvuII CAG'CTG
Cuts at: 174 521 717 1855 2215 174
Size: 347 196 1138 360 2389
Fragments arranged by size:
2389 1138 360 347 196
RsrII CG'GwC_CG
Cuts at: 2625 2625
Size: 4430
SacI G_AGCT'C
Cuts at: 1444 1444
Size: 4430
SalI G'TCGA_C
Cuts at: 1467 1467
Size: 4430
SapI GCTCTTCn'nnn_
Cuts at: 766 2453 2663 766
Size: 1687 210 2533
Fragments arranged by size:
2533 1687 210
SbfI CC_TGCA'GG
Cuts at: 1477 1477
Size: 4430
ScaI AGT'ACT
Cuts at: 3947 3947
Size: 4430
SexAI A'CCwGG_T
Cuts at: 1202 1202
Size: 4430
SfcI C'TryA_G
Cuts at: 33 127 844 1168 1473 2158 3151 3342 33
Size: 94 717 324 305 685 993 191 1121
Fragments arranged by size:
1121 993 717 685 324 305 191 94
SfoI GGC'GCC
Cuts at: 1862 2110 1862
Size: 248 4182
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4430
SmaI CCC'GGG
Cuts at: 1452 1452
Size: 4430
SmlI C'TyrA_G
Cuts at: 1060 1336 1585 2500 2992 3254 1060
Size: 276 249 915 492 262 2236
Fragments arranged by size:
2236 915 492 276 262 249
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4430
SphI G_CATG'C
Cuts at: 1483 1983 1483
Size: 500 3930
StuI AGG'CCT
Cuts at: 577 577
Size: 4430
StyI C'CwwG_G
Cuts at: 107 143 1316 1386 1564 1918 107
Size: 36 1173 70 178 354 2619
Fragments arranged by size:
2619 1173 354 178 70 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 982 2013 2146 2605 2814 982
Size: 1031 133 459 209 2598
Fragments arranged by size:
2598 1031 459 209 133
TatI w'GTAC_w
Cuts at: 557 581 891 995 1028 3945 557
Size: 24 310 104 33 2917 1042
Fragments arranged by size:
2917 1042 310 104 33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1157 1189 3947 3979 1157
Size: 32 2758 32 1608
Fragments arranged by size:
2758 1608 32 32
Tth111I GACn'n_nGTC
Cuts at: 2227 2227
Size: 4430
XbaI T'CTAG_A
Cuts at: 1461 1461
Size: 4430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 969 1278 1374 1925 969
Size: 309 96 551 3474
Fragments arranged by size:
3474 551 309 96
XmaI C'CCGG_G
Cuts at: 1450 1450
Size: 4430
XmnI GAAnn'nnTTC
Cuts at: 664 664
Size: 4430
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI ApaI AscI AvrII
BbsI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI