Restriction map for
pF1KB6627
(Circular) (Six-base) MAPSORT of: pF1KB6627.seq Check: 7782 from: 1 to: 4358
pF1KB6627 4358 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 233 575 713 1028 1396 233
Size: 342 138 315 368 3195
Fragments arranged by size:
3195 368 342 315 138
Acc65I G'GTAC_C
Cuts at: 1374 1374
Size: 4358
AclI AA'CG_TT
Cuts at: 4198 4198
Size: 4358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 265 686 1031 1184 1229 2183 2615 3362 3630
Size: 421 345 153 45 954 432 747 268
Cuts at: 3630 265
Size: 993
Fragments arranged by size:
993 954 747 432 421 345 268 153
45
AflIII A'CryG_T
Cuts at: 1710 2814 3791 1710
Size: 1104 977 2277
Fragments arranged by size:
2277 1104 977
AgeI A'CCGG_T
Cuts at: 2746 2746
Size: 4358
AlwNI CAG_nnn'CTG
Cuts at: 1219 1663 3230 1219
Size: 444 1567 2347
Fragments arranged by size:
2347 1567 444
ApaLI G'TGCA_C
Cuts at: 3128 3128
Size: 4358
ApoI r'AATT_y
Cuts at: 1362 3711 3967 1362
Size: 2349 256 1753
Fragments arranged by size:
2349 1753 256
AseI AT'TA_AT
Cuts at: 20 614 20
Size: 594 3764
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
AvaI C'yCGr_G
Cuts at: 1378 1378
Size: 4358
BamHI G'GATC_C
Cuts at: 1383 1383
Size: 4358
BanI G'GyrC_C
Cuts at: 1374 1788 2036 2071 1374
Size: 414 248 35 3661
Fragments arranged by size:
3661 414 248 35
BanII G_rGCy'C
Cuts at: 244 1372 2402 244
Size: 1128 1030 2200
Fragments arranged by size:
2200 1128 1030
BbeI G_GCGC'C
Cuts at: 1792 2040 1792
Size: 248 4110
BbsI GAAGACnn'nnnn_
Cuts at: 266 854 266
Size: 588 3770
BciVI GTATCCnnnnn_n'
Cuts at: 678 832 2256 3017 4358 678
Size: 154 1424 761 1341 678
Fragments arranged by size:
1424 1341 761 678 154
BclI T'GATC_A
Cuts at: 1878 1878
Size: 4358
BfrBI ATG'CAT
Cuts at: 454 1305 454
Size: 851 3507
BglII A'GATC_T
Cuts at: 1873 3910 1873
Size: 2037 2321
BlpI GC'TnA_GC
Cuts at: 788 1470 788
Size: 682 3676
Bme1580I G_kGCm'C
Cuts at: 1207 1983 2076 3132 1207
Size: 776 93 1056 2433
Fragments arranged by size:
2433 1056 776 93
BmrI ACTGGGnnnn_n'
Cuts at: 1032 1570 1651 1986 4130 1032
Size: 538 81 335 2144 1260
Fragments arranged by size:
2144 1260 538 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2725 2725
Size: 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 308 905 228
Size: 80 597 3681
Fragments arranged by size:
3681 597 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1534 2413 2905 3203 1534
Size: 879 492 298 2689
Fragments arranged by size:
2689 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4062 4062
Size: 4358
BsaAI yAC'GTr
Cuts at: 1711 1711
Size: 4358
BsaBI GATnn'nnATC
Cuts at: 6 1872 3909 6
Size: 1866 2037 455
Fragments arranged by size:
2037 1866 455
BsaHI Gr'CG_yC
Cuts at: 1100 1789 2037 2739 4235 1100
Size: 689 248 702 1496 1223
Fragments arranged by size:
1496 1223 702 689 248
BsaWI w'CCGG_w
Cuts at: 1547 2068 2746 3020 3167 3578 1547
Size: 521 678 274 147 411 2327
Fragments arranged by size:
2327 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 226 256 226
Size: 30 4328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 1213 546
Size: 667 3691
BseYI C'CCAG_C
Cuts at: 1783 2541 3118 3614 1783
Size: 758 577 496 2527
Fragments arranged by size:
2527 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 611 611
Size: 4358
BsiEI CG_ry'CG
Cuts at: 104 1900 1946 3154 104
Size: 1796 46 1208 1308
Fragments arranged by size:
1796 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1372 2150 2340 3132 1372
Size: 778 190 792 2598
Fragments arranged by size:
2598 792 778 190
BsmI GAATG_Cn'
Cuts at: 711 711
Size: 4358
Bsp1286I G_dGCh'C
Cuts at: 244 1207 1372 1983 2076 2150 2340 2402 3132
Size: 963 165 611 93 74 190 62 730
Cuts at: 3132 244
Size: 1470
Fragments arranged by size:
1470 963 730 611 190 165 93 74
62
BspEI T'CCGG_A
Cuts at: 3578 3578
Size: 4358
BspHI T'CATG_A
Cuts at: 3534 3534
Size: 4358
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1408 1622 1924 2305 1408
Size: 214 302 381 3461
Fragments arranged by size:
3461 381 302 214
BsrBI CCG'CTC
Cuts at: 50 431 2650 2704 4188 4353 50
Size: 381 2219 54 1484 165 55
Fragments arranged by size:
2219 1484 381 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2270 2270
Size: 4358
BsrFI r'CCGG_y
Cuts at: 2356 2537 2746 2356
Size: 181 209 3968
Fragments arranged by size:
3968 209 181
BsrGI T'GTAC_A
Cuts at: 801 801
Size: 4358
BssSI C'ACGA_G
Cuts at: 2629 2987 2629
Size: 358 4000
BstAPI GCAn_nnn'nTGC
Cuts at: 403 451 1735 403
Size: 48 1284 3026
Fragments arranged by size:
3026 1284 48
BstBI TT'CG_AA
Cuts at: 2719 2719
Size: 4358
BstXI CCAn_nnnn'nTGG
Cuts at: 228 942 228
Size: 714 3644
BstYI r'GATC_y
Cuts at: 1 114 359 1069 1383 1850 1873 2208 2454
Size: 113 245 710 314 467 23 335 246
Cuts at: 2454 3466 3552 3564 3910 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 710 467 449 346 335 314 246
245 113 86 23 12
BstZ17I GTA'TAC
Cuts at: 1029 1029
Size: 4358
BtgI C'CryG_G
Cuts at: 107 202 221 227 1181 107
Size: 95 19 6 954 3284
Fragments arranged by size:
3284 954 95 19 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2308 2489 2652 2308
Size: 181 163 4014
Fragments arranged by size:
4014 181 163
BtsI GCAGTG_nn'
Cuts at: 1601 1601
Size: 4358
DraI TTT'AAA
Cuts at: 1357 1357
Size: 4358
DrdI GACnn_nn'nnGTC
Cuts at: 2064 2922 2064
Size: 858 3500
EaeI y'GGCC_r
Cuts at: 224 269 1943 2117 2508 2535 3880 224
Size: 45 1674 174 391 27 1345 702
Fragments arranged by size:
1674 1345 702 391 174 45 27
EagI C'GGCC_G
Cuts at: 1943 1943
Size: 4358
EarI CTCTTCn'nnn_
Cuts at: 196 1046 2381 2591 196
Size: 850 1335 210 1963
Fragments arranged by size:
1963 1335 850 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2566 2876 3022 4168 2566
Size: 310 146 1146 2756
Fragments arranged by size:
2756 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1370 1370
Size: 4358
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 228 265 308 686 905 1031 1184 1229 2183
Size: 37 43 378 219 126 153 45 954
Cuts at: 2183 2615 3362 3630 228
Size: 432 747 268 956
Fragments arranged by size:
956 954 747 432 378 268 219 153
126 45 43 37
EcoO109I rG'GnC_Cy
Cuts at: 1497 1497
Size: 4358
EcoRI G'AATT_C
Cuts at: 1362 1362
Size: 4358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4358
FspI TGC'GCA
Cuts at: 2139 2139
Size: 4358
HaeII r_GCGC'y
Cuts at: 1792 2040 3062 4041 1792
Size: 248 1022 979 2109
Fragments arranged by size:
2109 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2200 2230 2232 2262 2200
Size: 30 2 30 4296
Fragments arranged by size:
4296 30 30 2
HincII GTy'rAC
Cuts at: 1397 1397
Size: 4358
KasI G'GCGC_C
Cuts at: 1788 2036 1788
Size: 248 4110
KpnI G_GTAC'C
Cuts at: 1378 1378
Size: 4358
MluI A'CGCG_T
Cuts at: 3791 3791
Size: 4358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1118 3029 3213 1118
Size: 1911 184 2263
Fragments arranged by size:
2263 1911 184
MscI TGG'CCA
Cuts at: 226 271 2119 226
Size: 45 1848 2465
Fragments arranged by size:
2465 1848 45
MslI CAynn'nnrTG
Cuts at: 226 1090 2347 2474 226
Size: 864 1257 127 2110
Fragments arranged by size:
2110 1257 864 127
MspA1I CmG'CkG
Cuts at: 341 1219 1469 1783 2143 3156 3401 341
Size: 878 250 314 360 1013 245 1298
Fragments arranged by size:
1298 1013 878 360 314 250 245
NaeI GCC'GGC
Cuts at: 2539 2539
Size: 4358
NarI GG'CG_CC
Cuts at: 1789 2037 1789
Size: 248 4110
NcoI C'CATG_G
Cuts at: 107 202 221 227 1181 107
Size: 95 19 6 954 3284
Fragments arranged by size:
3284 954 95 19 6
NdeI CA'TA_TG
Cuts at: 879 879
Size: 4358
NgoMIV G'CCGG_C
Cuts at: 2537 2537
Size: 4358
NsiI A_TGCA'T
Cuts at: 456 1307 456
Size: 851 3507
NspI r_CATG'y
Cuts at: 900 1411 1911 2818 900
Size: 511 500 907 2440
Fragments arranged by size:
2440 907 511 500
PciI A'CATG_T
Cuts at: 2814 2814
Size: 4358
PflMI CCAn_nnn'nTGG
Cuts at: 883 1182 883
Size: 299 4059
PfoI T'CCnGG_A
Cuts at: 518 518
Size: 4358
PmeI GTTT'AAAC
Cuts at: 1357 1357
Size: 4358
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 4326
PstI C_TGCA'G
Cuts at: 408 1405 2090 408
Size: 997 685 2676
Fragments arranged by size:
2676 997 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4358
PvuII CAG'CTG
Cuts at: 341 1783 2143 341
Size: 1442 360 2556
Fragments arranged by size:
2556 1442 360
RsrII CG'GwC_CG
Cuts at: 2553 2553
Size: 4358
SacI G_AGCT'C
Cuts at: 1372 1372
Size: 4358
SalI G'TCGA_C
Cuts at: 1395 1395
Size: 4358
SapI GCTCTTCn'nnn_
Cuts at: 1046 2381 2591 1046
Size: 1335 210 2813
Fragments arranged by size:
2813 1335 210
SbfI CC_TGCA'GG
Cuts at: 1405 1405
Size: 4358
ScaI AGT'ACT
Cuts at: 3875 3875
Size: 4358
SfcI C'TryA_G
Cuts at: 33 404 674 1401 2086 3079 3270 33
Size: 371 270 727 685 993 191 1121
Fragments arranged by size:
1121 993 727 685 371 270 191
SfoI GGC'GCC
Cuts at: 1790 2038 1790
Size: 248 4110
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4358
SmaI CCC'GGG
Cuts at: 1380 1380
Size: 4358
SmlI C'TyrA_G
Cuts at: 1513 2428 2920 3182 1513
Size: 915 492 262 2689
Fragments arranged by size:
2689 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4358
SphI G_CATG'C
Cuts at: 1411 1911 1411
Size: 500 3858
SspI AAT'ATT
Cuts at: 550 550
Size: 4358
StyI C'CwwG_G
Cuts at: 107 202 221 227 272 1181 1492 1846 107
Size: 95 19 6 45 909 311 354 2619
Fragments arranged by size:
2619 909 354 311 95 45 19 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 196 1941 2074 2533 2742 196
Size: 1745 133 459 209 1812
Fragments arranged by size:
1812 1745 459 209 133
TatI w'GTAC_w
Cuts at: 801 812 3873 801
Size: 11 3061 1286
Fragments arranged by size:
3061 1286 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3875 3907 3875
Size: 32 4326
Tth111I GACn'n_nGTC
Cuts at: 539 2155 539
Size: 1616 2742
XbaI T'CTAG_A
Cuts at: 867 1389 867
Size: 522 3836
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 225 234 696 1853 225
Size: 9 462 1157 2730
Fragments arranged by size:
2730 1157 462 9
XmaI C'CCGG_G
Cuts at: 1378 1378
Size: 4358
XmnI GAAnn'nnTTC
Cuts at: 190 530 190
Size: 340 4018
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BssHII BstEII Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI PacI PasI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI