Restriction map for
pF1KB6628
(Circular) (Six-base) MAPSORT of: pF1KB6628.seq Check: 7464 from: 1 to: 4331
pF1KB6628 4331 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1084 1084
Size: 4331
AccI GT'mk_AC
Cuts at: 381 999 1369 381
Size: 618 370 3343
Fragments arranged by size:
3343 618 370
Acc65I G'GTAC_C
Cuts at: 1347 1347
Size: 4331
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 2156 2588 3335 3603 851
Size: 1305 432 747 268 1579
Fragments arranged by size:
1579 1305 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 542 542
Size: 4331
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1264 1296 1264
Size: 32 4299
AlwNI CAG_nnn'CTG
Cuts at: 1077 1636 3203 1077
Size: 559 1567 2205
Fragments arranged by size:
2205 1567 559
ApaI G_GGCC'C
Cuts at: 966 1233 966
Size: 267 4064
ApaLI G'TGCA_C
Cuts at: 3101 3101
Size: 4331
ApoI r'AATT_y
Cuts at: 533 643 1335 3684 3940 533
Size: 110 692 2349 256 924
Fragments arranged by size:
2349 924 692 256 110
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 542 1351 542
Size: 809 3522
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 1347 1761 2009 2044 1347
Size: 414 248 35 3634
Fragments arranged by size:
3634 414 248 35
BanII G_rGCy'C
Cuts at: 860 966 1233 1345 2375 860
Size: 106 267 112 1030 2816
Fragments arranged by size:
2816 1030 267 112 106
BbeI G_GCGC'C
Cuts at: 1765 2013 1765
Size: 248 4083
BbsI GAAGACnn'nnnn_
Cuts at: 839 839
Size: 4331
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 252 286 252
Size: 34 4297
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 961 1189 961
Size: 228 4103
BglII A'GATC_T
Cuts at: 546 1846 3883 546
Size: 1300 2037 994
Fragments arranged by size:
2037 1300 994
BlpI GC'TnA_GC
Cuts at: 1443 1443
Size: 4331
Bme1580I G_kGCm'C
Cuts at: 957 966 1233 1956 2049 3105 957
Size: 9 267 723 93 1056 2183
Fragments arranged by size:
2183 1056 723 267 93 9
BmgBI CAC'GTC
Cuts at: 616 616
Size: 4331
BmrI ACTGGGnnnn_n'
Cuts at: 467 695 1278 1543 1624 1959 4103 467
Size: 228 583 265 81 335 2144 695
Fragments arranged by size:
2144 695 583 335 265 228 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 4331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 1284 571
Size: 713 3618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1507 2386 2878 3176 1507
Size: 879 492 298 2662
Fragments arranged by size:
2662 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 244 4035 244
Size: 3791 540
BsaAI yAC'GTr
Cuts at: 592 639 1684 592
Size: 47 1045 3239
Fragments arranged by size:
3239 1045 47
BsaBI GATnn'nnATC
Cuts at: 6 302 1845 3882 6
Size: 296 1543 2037 455
Fragments arranged by size:
2037 1543 455 296
BsaHI Gr'CG_yC
Cuts at: 1762 2010 2712 4208 1762
Size: 248 702 1496 1885
Fragments arranged by size:
1885 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1520 2041 2719 2993 3140 3551 1520
Size: 521 678 274 147 411 2300
Fragments arranged by size:
2300 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 309 321 339 351 1264 1294 309
Size: 12 18 12 913 30 3346
Fragments arranged by size:
3346 913 30 18 12 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 502 502
Size: 4331
BseYI C'CCAG_C
Cuts at: 371 699 1756 2514 3091 3587 371
Size: 328 1057 758 577 496 1115
Fragments arranged by size:
1115 1057 758 577 496 328
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 799 799
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1345 2123 2313 3105 1345
Size: 778 190 792 2571
Fragments arranged by size:
2571 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 860 957 966 1233 1345 1956 2049 2123 2313
Size: 97 9 267 112 611 93 74 190
Cuts at: 2313 2375 3105 860
Size: 62 730 2086
Fragments arranged by size:
2086 730 611 267 190 112 97 93
74 62 9
BspEI T'CCGG_A
Cuts at: 3551 3551
Size: 4331
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 776 1084 1381 1595 1897 2278 776
Size: 308 297 214 302 381 2829
Fragments arranged by size:
2829 381 308 302 297 214
BsrBI CCG'CTC
Cuts at: 50 911 2623 2677 4161 4326 50
Size: 861 1712 54 1484 165 55
Fragments arranged by size:
1712 1484 861 165 55 54
BsrDI GCAATG_nn'
Cuts at: 252 629 786 2243 252
Size: 377 157 1457 2340
Fragments arranged by size:
2340 1457 377 157
BsrFI r'CCGG_y
Cuts at: 2329 2510 2719 2329
Size: 181 209 3941
Fragments arranged by size:
3941 209 181
BsrGI T'GTAC_A
Cuts at: 717 717
Size: 4331
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 696 1708 696
Size: 1012 3319
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstYI r'GATC_y
Cuts at: 1 546 1080 1356 1823 1846 2181 2427 3439
Size: 545 534 276 467 23 335 246 1012
Cuts at: 3439 3525 3537 3883 1
Size: 86 12 346 449
Fragments arranged by size:
1012 545 534 467 449 346 335 276
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 485 485
Size: 4331
BtgI C'CryG_G
Cuts at: 107 755 1322 107
Size: 648 567 3116
Fragments arranged by size:
3116 648 567
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 599 2281 2462 2625 599
Size: 1682 181 163 2305
Fragments arranged by size:
2305 1682 181 163
BtsI GCAGTG_nn'
Cuts at: 355 1154 1574 355
Size: 799 420 3112
Fragments arranged by size:
3112 799 420
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 671 758 1014 1916 2090 2481 2508 3853 671
Size: 87 256 902 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 902 391 256 174 87 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 164 2354 2564 164
Size: 2190 210 1931
Fragments arranged by size:
2190 1931 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 851 1284 2156 2588 3335 3603 571
Size: 280 433 872 432 747 268 1299
Fragments arranged by size:
1299 872 747 433 432 280 268
EcoNI CCTnn'n_nnAGG
Cuts at: 772 889 772
Size: 117 4214
EcoO109I rG'GnC_Cy
Cuts at: 963 1229 1230 1470 963
Size: 266 1 240 3824
Fragments arranged by size:
3824 266 240 1
EcoRI G'AATT_C
Cuts at: 533 643 1335 533
Size: 110 692 3529
Fragments arranged by size:
3529 692 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 1136 1765 2013 3035 4014 1136
Size: 629 248 1022 979 1453
Fragments arranged by size:
1453 1022 979 629 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 270 302 309 321 341 353 547 579 595
Size: 32 7 12 20 12 194 32 16
Cuts at: 595 627 695 727 1072 1104 2173 2203 2205
Size: 32 68 32 345 32 1069 30 2
Cuts at: 2205 2235 270
Size: 30 2366
Fragments arranged by size:
2366 1069 345 194 68 32 32 32
32 32 30 30 20 16 12 12
7 2
HincII GTy'rAC
Cuts at: 382 1000 1370 382
Size: 618 370 3343
Fragments arranged by size:
3343 618 370
KasI G'GCGC_C
Cuts at: 1761 2009 1761
Size: 248 4083
KpnI G_GTAC'C
Cuts at: 1351 1351
Size: 4331
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 307 1167 3002 3186 307
Size: 860 1835 184 1452
Fragments arranged by size:
1835 1452 860 184
MscI TGG'CCA
Cuts at: 673 760 1016 2092 673
Size: 87 256 1076 2912
Fragments arranged by size:
2912 1076 256 87
MslI CAynn'nnrTG
Cuts at: 389 416 2320 2447 389
Size: 27 1904 127 2273
Fragments arranged by size:
2273 1904 127 27
MspA1I CmG'CkG
Cuts at: 693 1442 1756 2116 3129 3374 693
Size: 749 314 360 1013 245 1650
Fragments arranged by size:
1650 1013 749 360 314 245
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 1762 2010 1762
Size: 248 4083
NcoI C'CATG_G
Cuts at: 107 755 1322 107
Size: 648 567 3116
Fragments arranged by size:
3116 648 567
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NruI TCG'CGA
Cuts at: 399 584 399
Size: 185 4146
NspI r_CATG'y
Cuts at: 840 1384 1884 2791 840
Size: 544 500 907 2380
Fragments arranged by size:
2380 907 544 500
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 1077 1323 1077
Size: 246 4085
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1264 1296 3470 3502 1264
Size: 32 2174 32 2093
Fragments arranged by size:
2174 2093 32 32
PshAI GACnn'nnGTC
Cuts at: 413 413
Size: 4331
PspOMI G'GGCC_C
Cuts at: 962 1229 962
Size: 267 4064
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 563 595 563
Size: 32 4299
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1756 2116 1756
Size: 360 3971
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 380 998 1368 380
Size: 618 370 3343
Fragments arranged by size:
3343 618 370
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SfcI C'TryA_G
Cuts at: 33 569 1374 2059 3052 3243 33
Size: 536 805 685 993 191 1121
Fragments arranged by size:
1121 993 805 685 536 191
SfoI GGC'GCC
Cuts at: 1763 2011 1763
Size: 248 4083
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 542 1486 2401 2893 3155 542
Size: 944 915 492 262 1718
Fragments arranged by size:
1718 944 915 492 262
SnaBI TAC'GTA
Cuts at: 592 592
Size: 4331
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
SspI AAT'ATT
Cuts at: 879 879
Size: 4331
StyI C'CwwG_G
Cuts at: 107 755 1223 1322 1465 1819 107
Size: 648 468 99 143 354 2619
Fragments arranged by size:
2619 648 468 354 143 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1914 2047 2506 2715 1914
Size: 133 459 209 3530
Fragments arranged by size:
3530 459 209 133
TatI w'GTAC_w
Cuts at: 717 3846 717
Size: 3129 1202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3848 3880 3848
Size: 32 4299
Tth111I GACn'n_nGTC
Cuts at: 2128 2128
Size: 4331
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1826 1826
Size: 4331
XhoI C'TCGA_G
Cuts at: 542 542
Size: 4331
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PciI PflMI PmeI PpiI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AscI AvrII BaeI
BbvCI BfrBI BmtI Bpu10I BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NsiI
PacI PasI PfoI PmlI PpuMI PsiI PspXI SacII
SanDI SexAI SfiI SgrAI SrfI StuI SwaI XmnI
ZraI