Restriction map for
pF1KB6630
(Circular) (Six-base) MAPSORT of: pF1KB6630.seq Check: 9040 from: 1 to: 4433
pF1KB6630 4433 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1417 1417
Size: 4433
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 1347 2258 2690 3437 3705 547
Size: 800 911 432 747 268 1275
Fragments arranged by size:
1275 911 800 747 432 268
AflIII A'CryG_T
Cuts at: 1785 2889 3866 1785
Size: 1104 977 2352
Fragments arranged by size:
2352 1104 977
AgeI A'CCGG_T
Cuts at: 2821 2821
Size: 4433
AleI CACnn'nnGTG
Cuts at: 1331 1331
Size: 4433
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 481
Size: 32 4401
AlwNI CAG_nnn'CTG
Cuts at: 243 1251 1738 3305 243
Size: 1008 487 1567 1371
Fragments arranged by size:
1567 1371 1008 487
ApaLI G'TGCA_C
Cuts at: 3203 3203
Size: 4433
ApoI r'AATT_y
Cuts at: 1437 3786 4042 1437
Size: 2349 256 1828
Fragments arranged by size:
2349 1828 256
AseI AT'TA_AT
Cuts at: 20 1031 20
Size: 1011 3422
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 375 1453 375
Size: 1078 3355
BamHI G'GATC_C
Cuts at: 1458 1458
Size: 4433
BanI G'GyrC_C
Cuts at: 1325 1405 1449 1863 2111 2146 1325
Size: 80 44 414 248 35 3612
Fragments arranged by size:
3612 414 248 80 44 35
BanII G_rGCy'C
Cuts at: 189 1447 2477 189
Size: 1258 1030 2145
Fragments arranged by size:
2145 1258 1030
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BbsI GAAGACnn'nnnn_
Cuts at: 1170 1170
Size: 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4399
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3092 4433 2331
Size: 761 1341 2331
Fragments arranged by size:
2331 1341 761
BclI T'GATC_A
Cuts at: 1953 1953
Size: 4433
BglI GCCn_nnn'nGGC
Cuts at: 742 742
Size: 4433
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 283 675 1408 2058 2151 3207 283
Size: 392 733 650 93 1056 1509
Fragments arranged by size:
1509 1056 733 650 392 93
BmgBI CAC'GTC
Cuts at: 207 207
Size: 4433
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4205 1645
Size: 81 335 2144 1873
Fragments arranged by size:
2144 1873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 240 856 887 1318 203
Size: 37 616 31 431 3318
Fragments arranged by size:
3318 616 431 37 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 2980 3278 1609
Size: 879 492 298 2764
Fragments arranged by size:
2764 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 266 4137 266
Size: 3871 562
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 695 1864 2112 2814 4310 695
Size: 1169 248 702 1496 818
Fragments arranged by size:
1496 1169 818 702 248
BsaWI w'CCGG_w
Cuts at: 386 608 1622 2143 2821 3095 3242 3653 386
Size: 222 1014 521 678 274 147 411 1166
Fragments arranged by size:
1166 1014 678 521 411 274 222 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 547 577 547
Size: 30 4403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 552 699 826 552
Size: 147 127 4159
Fragments arranged by size:
4159 147 127
BseYI C'CCAG_C
Cuts at: 962 1858 2616 3193 3689 962
Size: 896 758 577 496 1706
Fragments arranged by size:
1706 896 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 411 606 1312 1330 411
Size: 195 706 18 3514
Fragments arranged by size:
3514 706 195 18
BsiEI CG_ry'CG
Cuts at: 104 1975 2021 3229 104
Size: 1871 46 1208 1308
Fragments arranged by size:
1871 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 704 1447 2225 2415 3207 704
Size: 743 778 190 792 1930
Fragments arranged by size:
1930 792 778 743 190
BsmI GAATG_Cn'
Cuts at: 376 886 376
Size: 510 3923
Bsp1286I G_dGCh'C
Cuts at: 189 283 675 704 1408 1447 2058 2151 2225
Size: 94 392 29 704 39 611 93 74
Cuts at: 2225 2415 2477 3207 189
Size: 190 62 730 1415
Fragments arranged by size:
1415 730 704 611 392 190 94 93
74 62 39 29
BspEI T'CCGG_A
Cuts at: 386 608 3653 386
Size: 222 3045 1166
Fragments arranged by size:
3045 1166 222
BspHI T'CATG_A
Cuts at: 3609 3609
Size: 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 530 1417 1483 1697 1999 2380 530
Size: 887 66 214 302 381 2583
Fragments arranged by size:
2583 887 381 302 214 66
BsrBI CCG'CTC
Cuts at: 50 2725 2779 4263 4428 50
Size: 2675 54 1484 165 55
Fragments arranged by size:
2675 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1327 2345 1327
Size: 1018 3415
BsrFI r'CCGG_y
Cuts at: 150 2431 2612 2821 150
Size: 2281 181 209 1762
Fragments arranged by size:
2281 1762 209 181
BsrGI T'GTAC_A
Cuts at: 542 542
Size: 4433
BssSI C'ACGA_G
Cuts at: 2704 3062 2704
Size: 358 4075
BstAPI GCAn_nnn'nTGC
Cuts at: 294 1810 294
Size: 1516 2917
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 1389 1389
Size: 4433
BstYI r'GATC_y
Cuts at: 1 1208 1458 1925 1948 2283 2529 3541 3627
Size: 1207 250 467 23 335 246 1012 86
Cuts at: 3627 3639 3985 1
Size: 12 346 449
Fragments arranged by size:
1207 1012 467 449 346 335 250 246
86 23 12
BtgI C'CryG_G
Cuts at: 548 1352 548
Size: 804 3629
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 746 2383 2564 2727 746
Size: 1637 181 163 2452
Fragments arranged by size:
2452 1637 181 163
BtsI GCAGTG_nn'
Cuts at: 618 1342 1676 618
Size: 724 334 3375
Fragments arranged by size:
3375 724 334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 202 237 202
Size: 35 4398
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DraIII CAC_nnn'GTG
Cuts at: 1353 1353
Size: 4433
DrdI GACnn_nn'nnGTC
Cuts at: 2139 2997 2139
Size: 858 3575
EaeI y'GGCC_r
Cuts at: 788 1127 1259 2018 2192 2583 2610 3955 788
Size: 339 132 759 174 391 27 1345 1266
Fragments arranged by size:
1345 1266 759 391 339 174 132 27
EagI C'GGCC_G
Cuts at: 2018 2018
Size: 4433
EarI CTCTTCn'nnn_
Cuts at: 221 345 501 709 2456 2666 221
Size: 124 156 208 1747 210 1988
Fragments arranged by size:
1988 1747 210 208 156 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 2951 3097 4243 2641
Size: 310 146 1146 2831
Fragments arranged by size:
2831 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1445 1445
Size: 4433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 203 240 547 856 887 1318 1347 2258 2690
Size: 37 307 309 31 431 29 911 432
Cuts at: 2690 3437 3705 203
Size: 747 268 931
Fragments arranged by size:
931 911 747 432 431 309 307 268
37 31 29
EcoNI CCTnn'n_nnAGG
Cuts at: 456 456
Size: 4433
EcoO109I rG'GnC_Cy
Cuts at: 1572 1572
Size: 4433
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 1125 1867 2115 3137 4116 1125
Size: 742 248 1022 979 1442
Fragments arranged by size:
1442 1022 979 742 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 427 459 2275 2305 2307 2337 427
Size: 32 1816 30 2 30 2523
Fragments arranged by size:
2523 1816 32 30 30 2
HincII GTy'rAC
Cuts at: 1472 1472
Size: 4433
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 524 704 3104 3288 524
Size: 180 2400 184 1669
Fragments arranged by size:
2400 1669 184 180
MscI TGG'CCA
Cuts at: 790 1129 2194 790
Size: 339 1065 3029
Fragments arranged by size:
3029 1065 339
MslI CAynn'nnrTG
Cuts at: 640 891 1331 2422 2549 640
Size: 251 440 1091 127 2524
Fragments arranged by size:
2524 1091 440 251 127
MspA1I CmG'CkG
Cuts at: 132 1215 1296 1544 1858 2218 3231 3476 132
Size: 1083 81 248 314 360 1013 245 1089
Fragments arranged by size:
1089 1083 1013 360 314 248 245 81
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 4433
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 4433
NspI r_CATG'y
Cuts at: 645 1486 1986 2893 645
Size: 841 500 907 2185
Fragments arranged by size:
2185 907 841 500
PciI A'CATG_T
Cuts at: 2889 2889
Size: 4433
PflMI CCAn_nnn'nTGG
Cuts at: 992 1251 992
Size: 259 4174
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 3572
Size: 32 4401
PsiI TTA'TAA
Cuts at: 1118 1118
Size: 4433
PstI C_TGCA'G
Cuts at: 137 248 1414 1480 2165 137
Size: 111 1166 66 685 2405
Fragments arranged by size:
2405 1166 685 111 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 1215 1296 1858 2218 1215
Size: 81 562 360 3430
Fragments arranged by size:
3430 562 360 81
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1447 1447
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SapI GCTCTTCn'nnn_
Cuts at: 221 709 2456 2666 221
Size: 488 1747 210 1988
Fragments arranged by size:
1988 1747 488 210
SbfI CC_TGCA'GG
Cuts at: 248 1414 1480 248
Size: 1166 66 3201
Fragments arranged by size:
3201 1166 66
ScaI AGT'ACT
Cuts at: 778 3950 778
Size: 3172 1261
SfcI C'TryA_G
Cuts at: 33 133 244 555 1410 1476 2161 3154 3345
Size: 100 111 311 855 66 685 993 191
Cuts at: 3345 33
Size: 1121
Fragments arranged by size:
1121 993 855 685 311 191 111 100
66
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1455 1455
Size: 4433
SmlI C'TyrA_G
Cuts at: 1588 2503 2995 3257 1588
Size: 915 492 262 2764
Fragments arranged by size:
2764 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StuI AGG'CCT
Cuts at: 462 818 462
Size: 356 4077
StyI C'CwwG_G
Cuts at: 588 1233 1376 1567 1921 588
Size: 645 143 191 354 3100
Fragments arranged by size:
3100 645 354 191 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 947 2016 2149 2608 2817 947
Size: 1069 133 459 209 2563
Fragments arranged by size:
2563 1069 459 209 133
TatI w'GTAC_w
Cuts at: 542 776 3948 542
Size: 234 3172 1027
Fragments arranged by size:
3172 1027 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 987 1019 1241 1273 3950 3982 987
Size: 32 222 32 2677 32 1438
Fragments arranged by size:
2677 1438 222 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2230 2230
Size: 4433
XbaI T'CTAG_A
Cuts at: 1464 1464
Size: 4433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 465 942 1928 465
Size: 477 986 2970
Fragments arranged by size:
2970 986 477
XmaI C'CCGG_G
Cuts at: 1453 1453
Size: 4433
XmnI GAAnn'nnTTC
Cuts at: 408 408
Size: 4433
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I ClaI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI