Restriction map for
pF1KB6633
(Circular) (Six-base) MAPSORT of: pF1KB6633.seq Check: 9348 from: 1 to: 4364
pF1KB6633 4364 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 255 255
Size: 4364
AccI GT'mk_AC
Cuts at: 1402 1402
Size: 4364
Acc65I G'GTAC_C
Cuts at: 701 1380 701
Size: 679 3685
AclI AA'CG_TT
Cuts at: 4204 4204
Size: 4364
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1076 2189 2621 3368 3636 1076
Size: 1113 432 747 268 1804
Fragments arranged by size:
1804 1113 747 432 268
AflIII A'CryG_T
Cuts at: 1716 2820 3797 1716
Size: 1104 977 2283
Fragments arranged by size:
2283 1104 977
AgeI A'CCGG_T
Cuts at: 2752 2752
Size: 4364
AleI CACnn'nnGTG
Cuts at: 787 787
Size: 4364
AlwNI CAG_nnn'CTG
Cuts at: 1669 3236 1669
Size: 1567 2797
ApaLI G'TGCA_C
Cuts at: 163 3134 163
Size: 2971 1393
ApoI r'AATT_y
Cuts at: 1368 3717 3973 1368
Size: 2349 256 1759
Fragments arranged by size:
2349 1759 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4364
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
AvaI C'yCGr_G
Cuts at: 136 321 1384 136
Size: 185 1063 3116
Fragments arranged by size:
3116 1063 185
BamHI G'GATC_C
Cuts at: 1389 1389
Size: 4364
BanI G'GyrC_C
Cuts at: 226 233 325 520 701 1380 1794 2042 2077
Size: 7 92 195 181 679 414 248 35
Cuts at: 2077 226
Size: 2513
Fragments arranged by size:
2513 679 414 248 195 181 92 35
7
BanII G_rGCy'C
Cuts at: 292 510 1378 2408 292
Size: 218 868 1030 2248
Fragments arranged by size:
2248 1030 868 218
BbeI G_GCGC'C
Cuts at: 230 237 329 524 1798 2046 230
Size: 7 92 195 1274 248 2548
Fragments arranged by size:
2548 1274 248 195 92 7
BbsI GAAGACnn'nnnn_
Cuts at: 1091 1091
Size: 4364
BbvCI CC'TCA_GC
Cuts at: 305 305
Size: 4364
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4330
BciVI GTATCCnnnnn_n'
Cuts at: 706 2262 3023 4364 706
Size: 1556 761 1341 706
Fragments arranged by size:
1556 1341 761 706
BclI T'GATC_A
Cuts at: 879 1884 879
Size: 1005 3359
BglII A'GATC_T
Cuts at: 1879 3916 1879
Size: 2037 2327
BlpI GC'TnA_GC
Cuts at: 280 1476 280
Size: 1196 3168
Bme1580I G_kGCm'C
Cuts at: 167 1989 2082 3138 167
Size: 1822 93 1056 1393
Fragments arranged by size:
1822 1393 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1576 1657 1992 4136 1576
Size: 81 335 2144 1804
Fragments arranged by size:
2144 1804 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2731 2731
Size: 4364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 458
Size: 4364
Bpu10I CC'TnA_GC
Cuts at: 305 305
Size: 4364
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1540 2419 2911 3209 1540
Size: 879 492 298 2695
Fragments arranged by size:
2695 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 745 4068 745
Size: 3323 1041
BsaAI yAC'GTr
Cuts at: 785 789 1717 785
Size: 4 928 3432
Fragments arranged by size:
3432 928 4
BsaBI GATnn'nnATC
Cuts at: 6 878 1878 3915 6
Size: 872 1000 2037 455
Fragments arranged by size:
2037 1000 872 455
BsaHI Gr'CG_yC
Cuts at: 227 234 326 521 778 1795 2043 2745 4241
Size: 7 92 195 257 1017 248 702 1496
Cuts at: 4241 227
Size: 350
Fragments arranged by size:
1496 1017 702 350 257 248 195 92
7
BsaWI w'CCGG_w
Cuts at: 1553 2074 2752 3026 3173 3584 1553
Size: 521 678 274 147 411 2333
Fragments arranged by size:
2333 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1059 1059
Size: 4364
BseYI C'CCAG_C
Cuts at: 359 586 943 1252 1789 2547 3124 3620 359
Size: 227 357 309 537 758 577 496 1103
Fragments arranged by size:
1103 758 577 537 496 357 309 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 811 1115 1196 1326 362
Size: 449 304 81 130 3400
Fragments arranged by size:
3400 449 304 130 81
BsiEI CG_ry'CG
Cuts at: 104 428 583 1906 1952 3160 104
Size: 324 155 1323 46 1208 1308
Fragments arranged by size:
1323 1308 1208 324 155 46
BsiHKAI G_wGCw'C
Cuts at: 131 167 1378 2156 2346 3138 131
Size: 36 1211 778 190 792 1357
Fragments arranged by size:
1357 1211 792 778 190 36
BsiWI C'GTAC_G
Cuts at: 785 785
Size: 4364
Bsp1286I G_dGCh'C
Cuts at: 131 167 292 510 1378 1989 2082 2156 2346
Size: 36 125 218 868 611 93 74 190
Cuts at: 2346 2408 3138 131
Size: 62 730 1357
Fragments arranged by size:
1357 868 730 611 218 190 125 93
74 62 36
BspEI T'CCGG_A
Cuts at: 3584 3584
Size: 4364
BspHI T'CATG_A
Cuts at: 3540 3540
Size: 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 255 584 689 1031 1414 1628 1930 2311 255
Size: 329 105 342 383 214 302 381 2308
Fragments arranged by size:
2308 383 381 342 329 302 214 105
BsrBI CCG'CTC
Cuts at: 50 2656 2710 4194 4359 50
Size: 2606 54 1484 165 55
Fragments arranged by size:
2606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2276 2276
Size: 4364
BsrFI r'CCGG_y
Cuts at: 184 213 427 704 2362 2543 2752 184
Size: 29 214 277 1658 181 209 1796
Fragments arranged by size:
1796 1658 277 214 209 181 29
BsrGI T'GTAC_A
Cuts at: 806 806
Size: 4364
BssHII G'CGCG_C
Cuts at: 155 528 155
Size: 373 3991
BssSI C'ACGA_G
Cuts at: 2635 2993 2635
Size: 358 4006
BstAPI GCAn_nnn'nTGC
Cuts at: 1129 1741 1129
Size: 612 3752
BstBI TT'CG_AA
Cuts at: 2725 2725
Size: 4364
BstXI CCAn_nnnn'nTGG
Cuts at: 594 1260 594
Size: 666 3698
BstYI r'GATC_y
Cuts at: 1 242 1389 1856 1879 2214 2460 3472 3558
Size: 241 1147 467 23 335 246 1012 86
Cuts at: 3558 3570 3916 1
Size: 12 346 449
Fragments arranged by size:
1147 1012 467 449 346 335 246 241
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1335 1335
Size: 4364
BtgI C'CryG_G
Cuts at: 407 407
Size: 4364
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2314 2495 2658 2314
Size: 181 163 4020
Fragments arranged by size:
4020 181 163
BtsI GCAGTG_nn'
Cuts at: 165 1607 165
Size: 1442 2922
DraI TTT'AAA
Cuts at: 1363 1363
Size: 4364
DraIII CAC_nnn'GTG
Cuts at: 888 888
Size: 4364
DrdI GACnn_nn'nnGTC
Cuts at: 2070 2928 2070
Size: 858 3506
EaeI y'GGCC_r
Cuts at: 186 425 450 580 755 842 1949 2123 2514
Size: 239 25 130 175 87 1107 174 391
Cuts at: 2514 2541 3886 186
Size: 27 1345 664
Fragments arranged by size:
1345 1107 664 391 239 175 174 130
87 27 25
EagI C'GGCC_G
Cuts at: 425 580 1949 425
Size: 155 1369 2840
Fragments arranged by size:
2840 1369 155
EarI CTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 345 841 2572 2882 3028 4174 345
Size: 496 1731 310 146 1146 535
Fragments arranged by size:
1731 1146 535 496 310 146
EcoICRI GAG'CTC
Cuts at: 1376 1376
Size: 4364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 1076 2189 2621 3368 3636 458
Size: 618 1113 432 747 268 1186
Fragments arranged by size:
1186 1113 747 618 432 268
EcoO109I rG'GnC_Cy
Cuts at: 318 1503 318
Size: 1185 3179
EcoRI G'AATT_C
Cuts at: 1368 1368
Size: 4364
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2383 2383
Size: 4364
FspI TGC'GCA
Cuts at: 2145 2145
Size: 4364
HaeII r_GCGC'y
Cuts at: 230 237 312 329 524 550 1798 2046 3068
Size: 7 75 17 195 26 1248 248 1022
Cuts at: 3068 4047 230
Size: 979 547
Fragments arranged by size:
1248 1022 979 547 248 195 75 26
17 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2206 2236 2238 2268 2206
Size: 30 2 30 4302
Fragments arranged by size:
4302 30 30 2
HincII GTy'rAC
Cuts at: 1403 1403
Size: 4364
KasI G'GCGC_C
Cuts at: 226 233 325 520 1794 2042 226
Size: 7 92 195 1274 248 2548
Fragments arranged by size:
2548 1274 248 195 92 7
KpnI G_GTAC'C
Cuts at: 705 1384 705
Size: 679 3685
MluI A'CGCG_T
Cuts at: 3797 3797
Size: 4364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3035 3219 3035
Size: 184 4180
MscI TGG'CCA
Cuts at: 844 2125 844
Size: 1281 3083
MslI CAynn'nnrTG
Cuts at: 787 1328 2353 2480 787
Size: 541 1025 127 2671
Fragments arranged by size:
2671 1025 541 127
MspA1I CmG'CkG
Cuts at: 409 448 1475 1789 2149 3162 3407 409
Size: 39 1027 314 360 1013 245 1366
Fragments arranged by size:
1366 1027 1013 360 314 245 39
NaeI GCC'GGC
Cuts at: 186 215 429 2545 186
Size: 29 214 2116 2005
Fragments arranged by size:
2116 2005 214 29
NarI GG'CG_CC
Cuts at: 227 234 326 521 1795 2043 227
Size: 7 92 195 1274 248 2548
Fragments arranged by size:
2548 1274 248 195 92 7
NgoMIV G'CCGG_C
Cuts at: 184 213 427 2543 184
Size: 29 214 2116 2005
Fragments arranged by size:
2116 2005 214 29
NotI GC'GGCC_GC
Cuts at: 580 580
Size: 4364
NspI r_CATG'y
Cuts at: 1127 1417 1917 2824 1127
Size: 290 500 907 2667
Fragments arranged by size:
2667 907 500 290
PciI A'CATG_T
Cuts at: 2820 2820
Size: 4364
PflMI CCAn_nnn'nTGG
Cuts at: 999 999
Size: 4364
PmeI GTTT'AAAC
Cuts at: 1363 1363
Size: 4364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 3503
Size: 32 4332
PpuMI rG'GwC_Cy
Cuts at: 318 318
Size: 4364
PstI C_TGCA'G
Cuts at: 1411 2096 1411
Size: 685 3679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4364
PvuII CAG'CTG
Cuts at: 1789 2149 1789
Size: 360 4004
RsrII CG'GwC_CG
Cuts at: 2559 2559
Size: 4364
SacI G_AGCT'C
Cuts at: 1378 1378
Size: 4364
SacII CC_GC'GG
Cuts at: 410 410
Size: 4364
SalI G'TCGA_C
Cuts at: 1401 1401
Size: 4364
SanDI GG'GwC_CC
Cuts at: 318 318
Size: 4364
SapI GCTCTTCn'nnn_
Cuts at: 2387 2597 2387
Size: 210 4154
SbfI CC_TGCA'GG
Cuts at: 1411 1411
Size: 4364
ScaI AGT'ACT
Cuts at: 637 3881 637
Size: 3244 1120
SexAI A'CCwGG_T
Cuts at: 749 1259 749
Size: 510 3854
SfcI C'TryA_G
Cuts at: 33 1407 2092 3085 3276 33
Size: 1374 685 993 191 1121
Fragments arranged by size:
1374 1121 993 685 191
SfoI GGC'GCC
Cuts at: 228 235 327 522 1796 2044 228
Size: 7 92 195 1274 248 2548
Fragments arranged by size:
2548 1274 248 195 92 7
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4364
SgrAI Cr'CCGG_yG
Cuts at: 213 213
Size: 4364
SmaI CCC'GGG
Cuts at: 323 1386 323
Size: 1063 3301
SmlI C'TyrA_G
Cuts at: 1519 2434 2926 3188 1519
Size: 915 492 262 2695
Fragments arranged by size:
2695 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4364
SphI G_CATG'C
Cuts at: 1417 1917 1417
Size: 500 3864
StyI C'CwwG_G
Cuts at: 921 1498 1852 921
Size: 577 354 3433
Fragments arranged by size:
3433 577 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1054 1947 2080 2539 2748 1054
Size: 893 133 459 209 2670
Fragments arranged by size:
2670 893 459 209 133
TatI w'GTAC_w
Cuts at: 635 806 847 1126 3879 635
Size: 171 41 279 2753 1120
Fragments arranged by size:
2753 1120 279 171 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3881 3913 3881
Size: 32 4332
Tth111I GACn'n_nGTC
Cuts at: 2161 2161
Size: 4364
XbaI T'CTAG_A
Cuts at: 502 1395 502
Size: 893 3471
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 959 1859 959
Size: 900 3464
XmaI C'CCGG_G
Cuts at: 321 1384 321
Size: 1063 3301
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI NspI
PciI PflMI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI BsmI
BsmBI BstEII BstZ17I ClaI CspCI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspOMI
PspXI PsrI SfiI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI