Restriction map for
pF1KB6635
(Circular) (Six-base) MAPSORT of: pF1KB6635.seq Check: 1442 from: 1 to: 4367
pF1KB6635 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 231 231
Size: 4367
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2192 2624 3371 3639 1318
Size: 874 432 747 268 2046
Fragments arranged by size:
2046 874 747 432 268
AflII C'TTAA_G
Cuts at: 1041 1041
Size: 4367
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AleI CACnn'nnGTG
Cuts at: 860 860
Size: 4367
AlwNI CAG_nnn'CTG
Cuts at: 528 1326 1672 3239 528
Size: 798 346 1567 1656
Fragments arranged by size:
1656 1567 798 346
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 283 315 358 505 627 1192 1269 1371 3720
Size: 32 43 147 122 565 77 102 2349
Cuts at: 3720 3976 283
Size: 256 674
Fragments arranged by size:
2349 674 565 256 147 122 102 77
43 32
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1387 1387
Size: 4367
BamHI G'GATC_C
Cuts at: 1392 1392
Size: 4367
BanI G'GyrC_C
Cuts at: 1383 1797 2045 2080 1383
Size: 414 248 35 3670
Fragments arranged by size:
3670 414 248 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 401 565 630 704 1013 401
Size: 164 65 74 309 3755
Fragments arranged by size:
3755 309 164 74 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 1887 1887
Size: 4367
BfrBI ATG'CAT
Cuts at: 573 823 573
Size: 250 4117
BglII A'GATC_T
Cuts at: 216 1882 3919 216
Size: 1666 2037 664
Fragments arranged by size:
2037 1666 664
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1579 1660 1995 4139 1579
Size: 81 335 2144 1807
Fragments arranged by size:
2144 1807 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
Bpu10I CC'TnA_GC
Cuts at: 1061 1061
Size: 4367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1037 1543 2422 2914 3212 1037
Size: 506 879 492 298 2192
Fragments arranged by size:
2192 879 506 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4071 4071
Size: 4367
BsaAI yAC'GTr
Cuts at: 1720 1720
Size: 4367
BsaBI GATnn'nnATC
Cuts at: 6 1881 3918 6
Size: 1875 2037 455
Fragments arranged by size:
2037 1875 455
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1556 2077 2755 3029 3176 3587 1556
Size: 521 678 274 147 411 2336
Fragments arranged by size:
2336 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 725 755 1346 1376 725
Size: 30 591 30 3716
Fragments arranged by size:
3716 591 30 30
BseYI C'CCAG_C
Cuts at: 1792 2550 3127 3623 1792
Size: 758 577 496 2536
Fragments arranged by size:
2536 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 779 1381 2159 2349 3141 779
Size: 602 778 190 792 2005
Fragments arranged by size:
2005 792 778 602 190
BsmI GAATG_Cn'
Cuts at: 1258 1258
Size: 4367
Bsp1286I G_dGCh'C
Cuts at: 779 1381 1992 2085 2159 2349 2411 3141 779
Size: 602 611 93 74 190 62 730 2005
Fragments arranged by size:
2005 730 611 602 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3587 3587
Size: 4367
BspHI T'CATG_A
Cuts at: 372 3543 372
Size: 3171 1196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 231 394 1417 1631 1933 2314 231
Size: 163 1023 214 302 381 2284
Fragments arranged by size:
2284 1023 381 302 214 163
BsrBI CCG'CTC
Cuts at: 50 270 2659 2713 4197 4362 50
Size: 220 2389 54 1484 165 55
Fragments arranged by size:
2389 1484 220 165 55 54
BsrDI GCAATG_nn'
Cuts at: 307 2279 307
Size: 1972 2395
BsrFI r'CCGG_y
Cuts at: 121 2365 2546 2755 121
Size: 2244 181 209 1733
Fragments arranged by size:
2244 1733 209 181
BssHII G'CGCG_C
Cuts at: 111 111
Size: 4367
BssSI C'ACGA_G
Cuts at: 989 2638 2996 989
Size: 1649 358 2360
Fragments arranged by size:
2360 1649 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstYI r'GATC_y
Cuts at: 1 216 850 1065 1392 1859 1882 2217 2463
Size: 215 634 215 327 467 23 335 246
Cuts at: 2463 3475 3561 3573 3919 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 634 467 449 346 335 327 246
215 215 86 23 12
BtgI C'CryG_G
Cuts at: 192 192
Size: 4367
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 583 2317 2498 2661 583
Size: 1734 181 163 2289
Fragments arranged by size:
2289 1734 181 163
BtsI GCAGTG_nn'
Cuts at: 1349 1610 1349
Size: 261 4106
DraI TTT'AAA
Cuts at: 321 626 708 1031 1366 321
Size: 305 82 323 335 3322
Fragments arranged by size:
3322 335 323 305 82
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 1952 2126 2517 2544 3889 1952
Size: 174 391 27 1345 2430
Fragments arranged by size:
2430 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 179 612 1242 2390 2600 179
Size: 433 630 1148 210 1946
Fragments arranged by size:
1946 1148 630 433 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 2192 2624 3371 3639 1318
Size: 874 432 747 268 2046
Fragments arranged by size:
2046 874 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1506 1506
Size: 4367
EcoRI G'AATT_C
Cuts at: 505 1371 505
Size: 866 3501
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 709 2386 709
Size: 1677 2690
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 2209 2239 2241 2271 163
Size: 32 2014 30 2 30 2259
Fragments arranged by size:
2259 2014 32 30 30 2
HincII GTy'rAC
Cuts at: 1406 1406
Size: 4367
HindIII A'AGCT_T
Cuts at: 469 469
Size: 4367
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3038 3222 3038
Size: 184 4183
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 514 860 2356 2483 514
Size: 346 1496 127 2398
Fragments arranged by size:
2398 1496 346 127
MspA1I CmG'CkG
Cuts at: 194 1478 1792 2152 3165 3410 194
Size: 1284 314 360 1013 245 1151
Fragments arranged by size:
1284 1151 1013 360 314 245
NaeI GCC'GGC
Cuts at: 123 2548 123
Size: 2425 1942
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NgoMIV G'CCGG_C
Cuts at: 121 2546 121
Size: 2425 1942
NsiI A_TGCA'T
Cuts at: 575 825 575
Size: 250 4117
NspI r_CATG'y
Cuts at: 1306 1420 1920 2827 1306
Size: 114 500 907 2846
Fragments arranged by size:
2846 907 500 114
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3506 3538 3506
Size: 32 4335
PstI C_TGCA'G
Cuts at: 242 870 1331 1414 2099 242
Size: 628 461 83 685 2510
Fragments arranged by size:
2510 685 628 461 83
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 1792 2152 1792
Size: 360 4007
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SacII CC_GC'GG
Cuts at: 195 195
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 2390 2600 2390
Size: 210 4157
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SfcI C'TryA_G
Cuts at: 33 238 463 866 923 1156 1318 1327 1410
Size: 205 225 403 57 233 162 9 83
Cuts at: 1410 2095 3088 3279 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 403 233 225 205 191
162 83 57 9
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 1041 1052 1522 2437 2929 3191 1041
Size: 11 470 915 492 262 2217
Fragments arranged by size:
2217 915 492 470 262 11
SpeI A'CTAG_T
Cuts at: 67 1092 67
Size: 1025 3342
SphI G_CATG'C
Cuts at: 1306 1420 1920 1306
Size: 114 500 3753
Fragments arranged by size:
3753 500 114
SspI AAT'ATT
Cuts at: 541 551 1036 541
Size: 10 485 3872
Fragments arranged by size:
3872 485 10
StuI AGG'CCT
Cuts at: 155 155
Size: 4367
StyI C'CwwG_G
Cuts at: 1501 1855 1501
Size: 354 4013
SwaI ATTT'AAAT
Cuts at: 626 626
Size: 4367
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1950 2083 2542 2751 1950
Size: 133 459 209 3566
Fragments arranged by size:
3566 459 209 133
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3884 3916 3884
Size: 32 4335
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
XmnI GAAnn'nnTTC
Cuts at: 888 888
Size: 4367
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NsiI NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BglI BmgBI BmtI BpmI BseRI BsgI
BsiWI BsmBI BsrGI BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SanDI SexAI SfiI SgrAI SnaBI SrfI XhoI ZraI