Restriction map for
pF1KB6637
(Circular) (Six-base) MAPSORT of: pF1KB6637.seq Check: 6438 from: 1 to: 4367
pF1KB6637 4367 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1405 1405
Size: 4367
Acc65I G'GTAC_C
Cuts at: 1383 1383
Size: 4367
AclI AA'CG_TT
Cuts at: 4207 4207
Size: 4367
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 571 1096 1098 1180 1235 2192 2624 3371
Size: 304 525 2 82 55 957 432 747
Cuts at: 3371 3639 267
Size: 268 995
Fragments arranged by size:
995 957 747 525 432 304 268 82
55 2
AflIII A'CryG_T
Cuts at: 1719 2823 3800 1719
Size: 1104 977 2286
Fragments arranged by size:
2286 1104 977
AgeI A'CCGG_T
Cuts at: 2755 2755
Size: 4367
AhdI GACnn_n'nnGTC
Cuts at: 1023 1023
Size: 4367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 154 168 154
Size: 14 4353
AlwNI CAG_nnn'CTG
Cuts at: 966 1672 3239 966
Size: 706 1567 2094
Fragments arranged by size:
2094 1567 706
ApaLI G'TGCA_C
Cuts at: 3137 3137
Size: 4367
ApoI r'AATT_y
Cuts at: 420 1371 3720 3976 420
Size: 951 2349 256 811
Fragments arranged by size:
2349 951 811 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4367
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
AvaI C'yCGr_G
Cuts at: 1264 1387 1264
Size: 123 4244
BamHI G'GATC_C
Cuts at: 183 1392 183
Size: 1209 3158
BanI G'GyrC_C
Cuts at: 831 1152 1383 1797 2045 2080 831
Size: 321 231 414 248 35 3118
Fragments arranged by size:
3118 414 321 248 231 35
BanII G_rGCy'C
Cuts at: 1381 2411 1381
Size: 1030 3337
BbeI G_GCGC'C
Cuts at: 1801 2049 1801
Size: 248 4119
BbsI GAAGACnn'nnnn_
Cuts at: 107 214 1022 1104 1284 107
Size: 107 808 82 180 3190
Fragments arranged by size:
3190 808 180 107 82
BbvCI CC'TCA_GC
Cuts at: 179 538 179
Size: 359 4008
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4333
BciVI GTATCCnnnnn_n'
Cuts at: 2265 3026 4367 2265
Size: 761 1341 2265
Fragments arranged by size:
2265 1341 761
BclI T'GATC_A
Cuts at: 228 1887 228
Size: 1659 2708
BglII A'GATC_T
Cuts at: 1882 3919 1882
Size: 2037 2330
BlpI GC'TnA_GC
Cuts at: 1479 1479
Size: 4367
Bme1580I G_kGCm'C
Cuts at: 1992 2085 3141 1992
Size: 93 1056 3218
Fragments arranged by size:
3218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 334 1105 1579 1660 1995 4139 334
Size: 771 474 81 335 2144 562
Fragments arranged by size:
2144 771 562 474 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 4367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 430 493 430
Size: 63 4304
Bpu10I CC'TnA_GC
Cuts at: 179 538 179
Size: 359 4008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 659 1543 2422 2914 3212 659
Size: 884 879 492 298 1814
Fragments arranged by size:
1814 884 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 759 4071 759
Size: 3312 1055
BsaAI yAC'GTr
Cuts at: 660 807 1720 660
Size: 147 913 3307
Fragments arranged by size:
3307 913 147
BsaBI GATnn'nnATC
Cuts at: 6 233 1881 3918 6
Size: 227 1648 2037 455
Fragments arranged by size:
2037 1648 455 227
BsaHI Gr'CG_yC
Cuts at: 1798 2046 2748 4244 1798
Size: 248 702 1496 1921
Fragments arranged by size:
1921 1496 702 248
BsaWI w'CCGG_w
Cuts at: 441 1556 2077 2755 3029 3176 3587 441
Size: 1115 521 678 274 147 411 1221
Fragments arranged by size:
1221 1115 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1305 1335 1305
Size: 30 4337
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 1162 466
Size: 696 3671
BseYI C'CCAG_C
Cuts at: 890 1222 1792 2550 3127 3623 890
Size: 332 570 758 577 496 1634
Fragments arranged by size:
1634 758 577 570 496 332
BsiEI CG_ry'CG
Cuts at: 104 1909 1955 3163 104
Size: 1805 46 1208 1308
Fragments arranged by size:
1805 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1381 2159 2349 3141 1381
Size: 778 190 792 2607
Fragments arranged by size:
2607 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1381 1992 2085 2159 2349 2411 3141 1381
Size: 611 93 74 190 62 730 2607
Fragments arranged by size:
2607 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 441 3587 441
Size: 3146 1221
BspHI T'CATG_A
Cuts at: 3543 3543
Size: 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1417 1631 1933 2314 1417
Size: 214 302 381 3470
Fragments arranged by size:
3470 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2659 2713 4197 4362 50
Size: 2609 54 1484 165 55
Fragments arranged by size:
2609 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 371 531 924 1125 2279 371
Size: 160 393 201 1154 2459
Fragments arranged by size:
2459 1154 393 201 160
BsrFI r'CCGG_y
Cuts at: 520 2365 2546 2755 520
Size: 1845 181 209 2132
Fragments arranged by size:
2132 1845 209 181
BssSI C'ACGA_G
Cuts at: 123 2638 2996 123
Size: 2515 358 1494
Fragments arranged by size:
2515 1494 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1744 1744
Size: 4367
BstBI TT'CG_AA
Cuts at: 2728 2728
Size: 4367
BstEII G'GTnAC_C
Cuts at: 816 816
Size: 4367
BstXI CCAn_nnnn'nTGG
Cuts at: 1005 1005
Size: 4367
BstYI r'GATC_y
Cuts at: 1 183 452 1392 1859 1882 2217 2463 3475
Size: 182 269 940 467 23 335 246 1012
Cuts at: 3475 3561 3573 3919 1
Size: 86 12 346 449
Fragments arranged by size:
1012 940 467 449 346 335 269 246
182 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 333 2317 2498 2661 333
Size: 1984 181 163 2039
Fragments arranged by size:
2039 1984 181 163
BtsI GCAGTG_nn'
Cuts at: 1610 1610
Size: 4367
DraI TTT'AAA
Cuts at: 750 1366 750
Size: 616 3751
DrdI GACnn_nn'nnGTC
Cuts at: 2073 2931 2073
Size: 858 3509
EaeI y'GGCC_r
Cuts at: 1952 2126 2517 2544 3889 1952
Size: 174 391 27 1345 2430
Fragments arranged by size:
2430 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1952 1952
Size: 4367
EarI CTCTTCn'nnn_
Cuts at: 626 788 1072 1333 2390 2600 626
Size: 162 284 261 1057 210 2393
Fragments arranged by size:
2393 1057 284 261 210 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2575 2885 3031 4177 2575
Size: 310 146 1146 2765
Fragments arranged by size:
2765 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1379 1379
Size: 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 267 430 493 571 1096 1098 1180 1235 2192
Size: 163 63 78 525 2 82 55 957
Cuts at: 2192 2624 3371 3639 267
Size: 432 747 268 995
Fragments arranged by size:
995 957 747 525 432 268 163 82
78 63 55 2
EcoNI CCTnn'n_nnAGG
Cuts at: 178 541 178
Size: 363 4004
EcoO109I rG'GnC_Cy
Cuts at: 414 546 1506 414
Size: 132 960 3275
Fragments arranged by size:
3275 960 132
EcoRI G'AATT_C
Cuts at: 1371 1371
Size: 4367
EcoRV GAT'ATC
Cuts at: 990 990
Size: 4367
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 4367
FspI TGC'GCA
Cuts at: 2148 2148
Size: 4367
HaeII r_GCGC'y
Cuts at: 1801 2049 3071 4050 1801
Size: 248 1022 979 2118
Fragments arranged by size:
2118 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1009 1041 2209 2239 2241 2271 1009
Size: 32 1168 30 2 30 3105
Fragments arranged by size:
3105 1168 32 30 30 2
HincII GTy'rAC
Cuts at: 615 1093 1406 615
Size: 478 313 3576
Fragments arranged by size:
3576 478 313
KasI G'GCGC_C
Cuts at: 1797 2045 1797
Size: 248 4119
KpnI G_GTAC'C
Cuts at: 1387 1387
Size: 4367
MluI A'CGCG_T
Cuts at: 3800 3800
Size: 4367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 338 3038 3222 338
Size: 2700 184 1483
Fragments arranged by size:
2700 1483 184
MscI TGG'CCA
Cuts at: 2128 2128
Size: 4367
MslI CAynn'nnrTG
Cuts at: 1249 2356 2483 1249
Size: 1107 127 3133
Fragments arranged by size:
3133 1107 127
MspA1I CmG'CkG
Cuts at: 300 513 890 1478 1792 2152 3165 3410 300
Size: 213 377 588 314 360 1013 245 1257
Fragments arranged by size:
1257 1013 588 377 360 314 245 213
NaeI GCC'GGC
Cuts at: 2548 2548
Size: 4367
NarI GG'CG_CC
Cuts at: 1798 2046 1798
Size: 248 4119
NgoMIV G'CCGG_C
Cuts at: 2546 2546
Size: 4367
NspI r_CATG'y
Cuts at: 858 1420 1920 2827 858
Size: 562 500 907 2398
Fragments arranged by size:
2398 907 562 500
PasI CC'CwG_GG
Cuts at: 189 375 189
Size: 186 4181
PciI A'CATG_T
Cuts at: 2823 2823
Size: 4367
PfoI T'CCnGG_A
Cuts at: 407 467 1004 407
Size: 60 537 3770
Fragments arranged by size:
3770 537 60
PmeI GTTT'AAAC
Cuts at: 1366 1366
Size: 4367
PmlI CAC'GTG
Cuts at: 807 807
Size: 4367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1258 1290 3506 3538 1258
Size: 32 2216 32 2087
Fragments arranged by size:
2216 2087 32 32
PstI C_TGCA'G
Cuts at: 1414 2099 1414
Size: 685 3682
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4367
PvuII CAG'CTG
Cuts at: 300 513 890 1792 2152 300
Size: 213 377 902 360 2515
Fragments arranged by size:
2515 902 377 360 213
RsrII CG'GwC_CG
Cuts at: 2562 2562
Size: 4367
SacI G_AGCT'C
Cuts at: 1381 1381
Size: 4367
SalI G'TCGA_C
Cuts at: 1404 1404
Size: 4367
SapI GCTCTTCn'nnn_
Cuts at: 1072 2390 2600 1072
Size: 1318 210 2839
Fragments arranged by size:
2839 1318 210
SbfI CC_TGCA'GG
Cuts at: 1414 1414
Size: 4367
ScaI AGT'ACT
Cuts at: 3884 3884
Size: 4367
SexAI A'CCwGG_T
Cuts at: 812 812
Size: 4367
SfcI C'TryA_G
Cuts at: 33 353 958 1410 2095 3088 3279 33
Size: 320 605 452 685 993 191 1121
Fragments arranged by size:
1121 993 685 605 452 320 191
SfoI GGC'GCC
Cuts at: 1799 2047 1799
Size: 248 4119
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4367
SmaI CCC'GGG
Cuts at: 1389 1389
Size: 4367
SmlI C'TyrA_G
Cuts at: 674 1522 2437 2929 3191 674
Size: 848 915 492 262 1850
Fragments arranged by size:
1850 915 848 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4367
SphI G_CATG'C
Cuts at: 1420 1920 1420
Size: 500 3867
StuI AGG'CCT
Cuts at: 149 149
Size: 4367
StyI C'CwwG_G
Cuts at: 383 1169 1501 1855 383
Size: 786 332 354 2895
Fragments arranged by size:
2895 786 354 332
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 254 882 997 1369 1950 2083 2542 2751 254
Size: 628 115 372 581 133 459 209 1870
Fragments arranged by size:
1870 628 581 459 372 209 133 115
TatI w'GTAC_w
Cuts at: 3882 3882
Size: 4367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 423 455 1328 1360 3884 3916 423
Size: 32 873 32 2524 32 874
Fragments arranged by size:
2524 874 873 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2164 2164
Size: 4367
XbaI T'CTAG_A
Cuts at: 1398 1398
Size: 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1862 1862
Size: 4367
XmaI C'CCGG_G
Cuts at: 1387 1387
Size: 4367
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI
PmlI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BsgI BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I Bsu36I BtgI ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PflMI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI