Restriction map for
pF1KB6640
(Circular) (Six-base) MAPSORT of: pF1KB6640.seq Check: 9916 from: 1 to: 4439
pF1KB6640 4439 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 1477 1477
Size: 4439
Acc65I G'GTAC_C
Cuts at: 1455 1455
Size: 4439
AclI AA'CG_TT
Cuts at: 4279 4279
Size: 4439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 686 2264 2696 3443 3711 554
Size: 132 1578 432 747 268 1282
Fragments arranged by size:
1578 1282 747 432 268 132
AflIII A'CryG_T
Cuts at: 390 1791 2895 3872 390
Size: 1401 1104 977 957
Fragments arranged by size:
1401 1104 977 957
AgeI A'CCGG_T
Cuts at: 2827 2827
Size: 4439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 248 248
Size: 4439
AlwNI CAG_nnn'CTG
Cuts at: 1744 3311 1744
Size: 1567 2872
ApaLI G'TGCA_C
Cuts at: 3209 3209
Size: 4439
ApoI r'AATT_y
Cuts at: 818 827 1007 1272 1443 3792 4048 818
Size: 9 180 265 171 2349 256 1209
Fragments arranged by size:
2349 1209 265 256 180 171 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 4439
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
AvaI C'yCGr_G
Cuts at: 1459 1459
Size: 4439
BamHI G'GATC_C
Cuts at: 1464 1464
Size: 4439
BanI G'GyrC_C
Cuts at: 961 1455 1869 2117 2152 961
Size: 494 414 248 35 3248
Fragments arranged by size:
3248 494 414 248 35
BanII G_rGCy'C
Cuts at: 420 1453 2483 420
Size: 1033 1030 2376
Fragments arranged by size:
2376 1033 1030
BbeI G_GCGC'C
Cuts at: 1873 2121 1873
Size: 248 4191
BbsI GAAGACnn'nnnn_
Cuts at: 542 623 542
Size: 81 4358
BciVI GTATCCnnnnn_n'
Cuts at: 2337 3098 4439 2337
Size: 761 1341 2337
Fragments arranged by size:
2337 1341 761
BclI T'GATC_A
Cuts at: 1959 1959
Size: 4439
BglII A'GATC_T
Cuts at: 1954 3991 1954
Size: 2037 2402
BlpI GC'TnA_GC
Cuts at: 419 1551 419
Size: 1132 3307
Bme1580I G_kGCm'C
Cuts at: 1383 2064 2157 3213 1383
Size: 681 93 1056 2609
Fragments arranged by size:
2609 1056 681 93
BmrI ACTGGGnnnn_n'
Cuts at: 154 1651 1732 2067 4211 154
Size: 1497 81 335 2144 382
Fragments arranged by size:
2144 1497 382 335 81
BmtI G_CTAG'C
Cuts at: 655 655
Size: 4439
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2806 2806
Size: 4439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1615 2494 2986 3284 1615
Size: 879 492 298 2770
Fragments arranged by size:
2770 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 142 4143 142
Size: 4001 438
BsaAI yAC'GTr
Cuts at: 1378 1792 1378
Size: 414 4025
BsaBI GATnn'nnATC
Cuts at: 6 1953 3990 6
Size: 1947 2037 455
Fragments arranged by size:
2037 1947 455
BsaHI Gr'CG_yC
Cuts at: 1870 2118 2820 4316 1870
Size: 248 702 1496 1993
Fragments arranged by size:
1993 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1628 2149 2827 3101 3248 3659 1628
Size: 521 678 274 147 411 2408
Fragments arranged by size:
2408 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1415 1415
Size: 4439
BseYI C'CCAG_C
Cuts at: 1864 2622 3199 3695 1864
Size: 758 577 496 2608
Fragments arranged by size:
2608 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1981 2027 3235 104
Size: 1877 46 1208 1308
Fragments arranged by size:
1877 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1453 2231 2421 3213 1453
Size: 778 190 792 2679
Fragments arranged by size:
2679 792 778 190
BsmI GAATG_Cn'
Cuts at: 796 796
Size: 4439
Bsp1286I G_dGCh'C
Cuts at: 420 1383 1453 2064 2157 2231 2421 2483 3213
Size: 963 70 611 93 74 190 62 730
Cuts at: 3213 420
Size: 1646
Fragments arranged by size:
1646 963 730 611 190 93 74 70
62
BspEI T'CCGG_A
Cuts at: 3659 3659
Size: 4439
BspHI T'CATG_A
Cuts at: 3615 3615
Size: 4439
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1195 1489 1703 2005 2386 1195
Size: 294 214 302 381 3248
Fragments arranged by size:
3248 381 302 294 214
BsrBI CCG'CTC
Cuts at: 50 2731 2785 4269 4434 50
Size: 2681 54 1484 165 55
Fragments arranged by size:
2681 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2351 2351
Size: 4439
BsrFI r'CCGG_y
Cuts at: 2437 2618 2827 2437
Size: 181 209 4049
Fragments arranged by size:
4049 209 181
BsrGI T'GTAC_A
Cuts at: 1122 1122
Size: 4439
BssHII G'CGCG_C
Cuts at: 129 129
Size: 4439
BssSI C'ACGA_G
Cuts at: 2710 3068 2710
Size: 358 4081
BstAPI GCAn_nnn'nTGC
Cuts at: 373 1263 1816 373
Size: 890 553 2996
Fragments arranged by size:
2996 890 553
BstBI TT'CG_AA
Cuts at: 2800 2800
Size: 4439
BstYI r'GATC_y
Cuts at: 1 1464 1931 1954 2289 2535 3547 3633 3645
Size: 1463 467 23 335 246 1012 86 12
Cuts at: 3645 3991 1
Size: 346 449
Fragments arranged by size:
1463 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 119 119
Size: 4439
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2389 2570 2733 2389
Size: 181 163 4095
Fragments arranged by size:
4095 181 163
BtsI GCAGTG_nn'
Cuts at: 632 674 1682 632
Size: 42 1008 3389
Fragments arranged by size:
3389 1008 42
DraI TTT'AAA
Cuts at: 1438 1438
Size: 4439
DrdI GACnn_nn'nnGTC
Cuts at: 2145 3003 2145
Size: 858 3581
EaeI y'GGCC_r
Cuts at: 378 2024 2198 2589 2616 3961 378
Size: 1646 174 391 27 1345 856
Fragments arranged by size:
1646 1345 856 391 174 27
EagI C'GGCC_G
Cuts at: 2024 2024
Size: 4439
EarI CTCTTCn'nnn_
Cuts at: 702 911 2462 2672 702
Size: 209 1551 210 2469
Fragments arranged by size:
2469 1551 210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2647 2957 3103 4249 2647
Size: 310 146 1146 2837
Fragments arranged by size:
2837 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1451 1451
Size: 4439
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 554 686 2264 2696 3443 3711 554
Size: 132 1578 432 747 268 1282
Fragments arranged by size:
1578 1282 747 432 268 132
EcoNI CCTnn'n_nnAGG
Cuts at: 973 973
Size: 4439
EcoO109I rG'GnC_Cy
Cuts at: 1362 1578 1362
Size: 216 4223
EcoRI G'AATT_C
Cuts at: 1007 1443 1007
Size: 436 4003
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 909 2458 909
Size: 1549 2890
FspI TGC'GCA
Cuts at: 2220 2220
Size: 4439
HaeII r_GCGC'y
Cuts at: 1873 2121 3143 4122 1873
Size: 248 1022 979 2190
Fragments arranged by size:
2190 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 613 645 2281 2311 2313 2343 613
Size: 32 1636 30 2 30 2709
Fragments arranged by size:
2709 1636 32 30 30 2
HincII GTy'rAC
Cuts at: 1344 1478 1344
Size: 134 4305
KasI G'GCGC_C
Cuts at: 1869 2117 1869
Size: 248 4191
KpnI G_GTAC'C
Cuts at: 1459 1459
Size: 4439
MfeI C'AATT_G
Cuts at: 565 1298 565
Size: 733 3706
MluI A'CGCG_T
Cuts at: 3872 3872
Size: 4439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3110 3294 3110
Size: 184 4255
MscI TGG'CCA
Cuts at: 380 2200 380
Size: 1820 2619
MslI CAynn'nnrTG
Cuts at: 768 2428 2555 768
Size: 1660 127 2652
Fragments arranged by size:
2652 1660 127
MspA1I CmG'CkG
Cuts at: 121 1550 1864 2224 3237 3482 121
Size: 1429 314 360 1013 245 1078
Fragments arranged by size:
1429 1078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2620 2620
Size: 4439
NarI GG'CG_CC
Cuts at: 1870 2118 1870
Size: 248 4191
NgoMIV G'CCGG_C
Cuts at: 2618 2618
Size: 4439
NheI G'CTAG_C
Cuts at: 651 651
Size: 4439
NspI r_CATG'y
Cuts at: 394 1492 1992 2899 394
Size: 1098 500 907 1934
Fragments arranged by size:
1934 1098 907 500
PasI CC'CwG_GG
Cuts at: 811 811
Size: 4439
PciI A'CATG_T
Cuts at: 390 2895 390
Size: 2505 1934
PmeI GTTT'AAAC
Cuts at: 1438 1438
Size: 4439
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4407
PpuMI rG'GwC_Cy
Cuts at: 1362 1362
Size: 4439
PsiI TTA'TAA
Cuts at: 593 593
Size: 4439
PstI C_TGCA'G
Cuts at: 369 1486 2171 369
Size: 1117 685 2637
Fragments arranged by size:
2637 1117 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4439
PvuII CAG'CTG
Cuts at: 1864 2224 1864
Size: 360 4079
RsrII CG'GwC_CG
Cuts at: 2634 2634
Size: 4439
SacI G_AGCT'C
Cuts at: 1453 1453
Size: 4439
SacII CC_GC'GG
Cuts at: 122 122
Size: 4439
SalI G'TCGA_C
Cuts at: 1476 1476
Size: 4439
SapI GCTCTTCn'nnn_
Cuts at: 2462 2672 2462
Size: 210 4229
SbfI CC_TGCA'GG
Cuts at: 1486 1486
Size: 4439
ScaI AGT'ACT
Cuts at: 3956 3956
Size: 4439
SfcI C'TryA_G
Cuts at: 33 365 1482 2167 3160 3351 33
Size: 332 1117 685 993 191 1121
Fragments arranged by size:
1121 1117 993 685 332 191
SfoI GGC'GCC
Cuts at: 1871 2119 1871
Size: 248 4191
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4439
SmaI CCC'GGG
Cuts at: 1461 1461
Size: 4439
SmlI C'TyrA_G
Cuts at: 1594 2509 3001 3263 1594
Size: 915 492 262 2770
Fragments arranged by size:
2770 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1110 67
Size: 1043 3396
SphI G_CATG'C
Cuts at: 1492 1992 1492
Size: 500 3939
StyI C'CwwG_G
Cuts at: 1573 1927 1573
Size: 354 4085
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 156 2022 2155 2614 2823 156
Size: 1866 133 459 209 1772
Fragments arranged by size:
1866 1772 459 209 133
TatI w'GTAC_w
Cuts at: 493 920 1122 3954 493
Size: 427 202 2832 978
Fragments arranged by size:
2832 978 427 202
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3956 3988 3956
Size: 32 4407
Tth111I GACn'n_nGTC
Cuts at: 2236 2236
Size: 4439
XbaI T'CTAG_A
Cuts at: 1395 1470 1395
Size: 75 4364
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1934 1934
Size: 4439
XmaI C'CCGG_G
Cuts at: 1459 1459
Size: 4439
XmnI GAAnn'nnTTC
Cuts at: 892 1166 892
Size: 274 4165
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI NspI PasI PciI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BpmI Bpu10I BsaXI BsgI BsiWI BsmBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI NcoI NdeI NotI NruI NsiI PacI
PflMI PfoI PmlI PshAI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI