Restriction map for
pF1KB6643
(Circular) (Six-base) MAPSORT of: pF1KB6643.seq Check: 7020 from: 1 to: 4274
pF1KB6643 4274 bp
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AccI GT'mk_AC
Cuts at: 284 1312 284
Size: 1028 3246
Acc65I G'GTAC_C
Cuts at: 1290 1290
Size: 4274
AclI AA'CG_TT
Cuts at: 4114 4114
Size: 4274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 407 2099 2531 3278 3546 407
Size: 1692 432 747 268 1135
Fragments arranged by size:
1692 1135 747 432 268
AflIII A'CryG_T
Cuts at: 1626 2730 3707 1626
Size: 1104 977 2193
Fragments arranged by size:
2193 1104 977
AgeI A'CCGG_T
Cuts at: 2662 2662
Size: 4274
AhdI GACnn_n'nnGTC
Cuts at: 650 650
Size: 4274
AlwNI CAG_nnn'CTG
Cuts at: 1579 3146 1579
Size: 1567 2707
ApaI G_GGCC'C
Cuts at: 886 886
Size: 4274
ApaLI G'TGCA_C
Cuts at: 3044 3044
Size: 4274
ApoI r'AATT_y
Cuts at: 990 1278 3627 3883 990
Size: 288 2349 256 1381
Fragments arranged by size:
2349 1381 288 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4274
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
AvaI C'yCGr_G
Cuts at: 129 1294 129
Size: 1165 3109
BamHI G'GATC_C
Cuts at: 580 1299 580
Size: 719 3555
BanI G'GyrC_C
Cuts at: 1290 1704 1952 1987 1290
Size: 414 248 35 3577
Fragments arranged by size:
3577 414 248 35
BanII G_rGCy'C
Cuts at: 886 1288 2318 886
Size: 402 1030 2842
Fragments arranged by size:
2842 1030 402
BbeI G_GCGC'C
Cuts at: 1708 1956 1708
Size: 248 4026
BbsI GAAGACnn'nnnn_
Cuts at: 917 917
Size: 4274
BciVI GTATCCnnnnn_n'
Cuts at: 1151 2172 2933 4274 1151
Size: 1021 761 1341 1151
Fragments arranged by size:
1341 1151 1021 761
BclI T'GATC_A
Cuts at: 1794 1794
Size: 4274
BfrBI ATG'CAT
Cuts at: 726 1018 726
Size: 292 3982
BglII A'GATC_T
Cuts at: 1789 3826 1789
Size: 2037 2237
BlpI GC'TnA_GC
Cuts at: 1386 1386
Size: 4274
Bme1580I G_kGCm'C
Cuts at: 886 1899 1992 3048 886
Size: 1013 93 1056 2112
Fragments arranged by size:
2112 1056 1013 93
BmrI ACTGGGnnnn_n'
Cuts at: 1486 1567 1902 4046 1486
Size: 81 335 2144 1714
Fragments arranged by size:
2144 1714 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 407 2641 407
Size: 2234 2040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 938 389
Size: 549 3725
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1450 2329 2821 3119 1450
Size: 879 492 298 2605
Fragments arranged by size:
2605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3978 3978
Size: 4274
BsaAI yAC'GTr
Cuts at: 1627 1627
Size: 4274
BsaBI GATnn'nnATC
Cuts at: 6 308 1788 3825 6
Size: 302 1480 2037 455
Fragments arranged by size:
2037 1480 455 302
BsaHI Gr'CG_yC
Cuts at: 1705 1953 2655 4151 1705
Size: 248 702 1496 1828
Fragments arranged by size:
1828 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1463 1984 2662 2936 3083 3494 1463
Size: 521 678 274 147 411 2243
Fragments arranged by size:
2243 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 863 863
Size: 4274
BseYI C'CCAG_C
Cuts at: 1055 1217 1699 2457 3034 3530 1055
Size: 162 482 758 577 496 1799
Fragments arranged by size:
1799 758 577 496 482 162
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4274
BsiEI CG_ry'CG
Cuts at: 104 1816 1862 3070 104
Size: 1712 46 1208 1308
Fragments arranged by size:
1712 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1288 2066 2256 3048 1288
Size: 778 190 792 2514
Fragments arranged by size:
2514 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 886 1288 1899 1992 2066 2256 2318 3048 886
Size: 402 611 93 74 190 62 730 2112
Fragments arranged by size:
2112 730 611 402 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3494 3494
Size: 4274
BspHI T'CATG_A
Cuts at: 3450 3450
Size: 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1257 1324 1538 1840 2221 1257
Size: 67 214 302 381 3310
Fragments arranged by size:
3310 381 302 214 67
BsrBI CCG'CTC
Cuts at: 50 386 2566 2620 4104 4269 50
Size: 336 2180 54 1484 165 55
Fragments arranged by size:
2180 1484 336 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2186 2186
Size: 4274
BsrFI r'CCGG_y
Cuts at: 2272 2453 2662 2272
Size: 181 209 3884
Fragments arranged by size:
3884 209 181
BssSI C'ACGA_G
Cuts at: 2545 2903 2545
Size: 358 3916
BstAPI GCAn_nnn'nTGC
Cuts at: 267 1651 267
Size: 1384 2890
BstBI TT'CG_AA
Cuts at: 2635 2635
Size: 4274
BstEII G'GTnAC_C
Cuts at: 1084 1084
Size: 4274
BstXI CCAn_nnnn'nTGG
Cuts at: 114 490 114
Size: 376 3898
BstYI r'GATC_y
Cuts at: 1 580 1299 1766 1789 2124 2370 3382 3468
Size: 579 719 467 23 335 246 1012 86
Cuts at: 3468 3480 3826 1
Size: 12 346 449
Fragments arranged by size:
1012 719 579 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 285 285
Size: 4274
BtgI C'CryG_G
Cuts at: 576 584 576
Size: 8 4266
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2224 2405 2568 2224
Size: 181 163 3930
Fragments arranged by size:
3930 181 163
BtsI GCAGTG_nn'
Cuts at: 1517 1517
Size: 4274
DraI TTT'AAA
Cuts at: 1273 1273
Size: 4274
DraIII CAC_nnn'GTG
Cuts at: 1123 1123
Size: 4274
DrdI GACnn_nn'nnGTC
Cuts at: 1980 2838 1980
Size: 858 3416
EaeI y'GGCC_r
Cuts at: 480 1002 1859 2033 2424 2451 3796 480
Size: 522 857 174 391 27 1345 958
Fragments arranged by size:
1345 958 857 522 391 174 27
EagI C'GGCC_G
Cuts at: 1859 1859
Size: 4274
EarI CTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 785 2482 2792 2938 4084 785
Size: 1697 310 146 1146 975
Fragments arranged by size:
1697 1146 975 310 146
EcoICRI GAG'CTC
Cuts at: 1286 1286
Size: 4274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 389 407 938 2099 2531 3278 3546 389
Size: 18 531 1161 432 747 268 1117
Fragments arranged by size:
1161 1117 747 531 432 268 18
EcoNI CCTnn'n_nnAGG
Cuts at: 956 956
Size: 4274
EcoO109I rG'GnC_Cy
Cuts at: 364 445 517 882 949 1132 1413 364
Size: 81 72 365 67 183 281 3225
Fragments arranged by size:
3225 365 281 183 81 72 67
EcoRI G'AATT_C
Cuts at: 1278 1278
Size: 4274
EcoRV GAT'ATC
Cuts at: 1048 1048
Size: 4274
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2293 2293
Size: 4274
FspI TGC'GCA
Cuts at: 2055 2055
Size: 4274
HaeII r_GCGC'y
Cuts at: 1708 1956 2978 3957 1708
Size: 248 1022 979 2025
Fragments arranged by size:
2025 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 636 668 2116 2146 2148 2178 636
Size: 32 1448 30 2 30 2732
Fragments arranged by size:
2732 1448 32 30 30 2
HincII GTy'rAC
Cuts at: 822 1313 822
Size: 491 3783
HpaI GTT'AAC
Cuts at: 822 822
Size: 4274
KasI G'GCGC_C
Cuts at: 1704 1952 1704
Size: 248 4026
KpnI G_GTAC'C
Cuts at: 1294 1294
Size: 4274
MluI A'CGCG_T
Cuts at: 3707 3707
Size: 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 2945 3129 161
Size: 2784 184 1306
Fragments arranged by size:
2784 1306 184
MscI TGG'CCA
Cuts at: 1004 2035 1004
Size: 1031 3243
MslI CAynn'nnrTG
Cuts at: 112 559 2263 2390 112
Size: 447 1704 127 1996
Fragments arranged by size:
1996 1704 447 127
MspA1I CmG'CkG
Cuts at: 163 1385 1699 2059 3072 3317 163
Size: 1222 314 360 1013 245 1120
Fragments arranged by size:
1222 1120 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2455 2455
Size: 4274
NarI GG'CG_CC
Cuts at: 1705 1953 1705
Size: 248 4026
NcoI C'CATG_G
Cuts at: 576 584 576
Size: 8 4266
NdeI CA'TA_TG
Cuts at: 503 836 503
Size: 333 3941
NgoMIV G'CCGG_C
Cuts at: 2453 2453
Size: 4274
NsiI A_TGCA'T
Cuts at: 728 1020 728
Size: 292 3982
NspI r_CATG'y
Cuts at: 420 465 627 1327 1827 2734 420
Size: 45 162 700 500 907 1960
Fragments arranged by size:
1960 907 700 500 162 45
PasI CC'CwG_GG
Cuts at: 1072 1072
Size: 4274
PciI A'CATG_T
Cuts at: 2730 2730
Size: 4274
PfoI T'CCnGG_A
Cuts at: 366 366
Size: 4274
PmeI GTTT'AAAC
Cuts at: 1273 1273
Size: 4274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3413 3445 3413
Size: 32 4242
PpuMI rG'GwC_Cy
Cuts at: 364 445 517 949 1132 364
Size: 81 72 432 183 3506
Fragments arranged by size:
3506 432 183 81 72
PspOMI G'GGCC_C
Cuts at: 882 882
Size: 4274
PstI C_TGCA'G
Cuts at: 1321 2006 1321
Size: 685 3589
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4274
PvuII CAG'CTG
Cuts at: 1699 2059 1699
Size: 360 3914
RsrII CG'GwC_CG
Cuts at: 2469 2469
Size: 4274
SacI G_AGCT'C
Cuts at: 1288 1288
Size: 4274
SalI G'TCGA_C
Cuts at: 1311 1311
Size: 4274
SapI GCTCTTCn'nnn_
Cuts at: 2297 2507 2297
Size: 210 4064
SbfI CC_TGCA'GG
Cuts at: 1321 1321
Size: 4274
ScaI AGT'ACT
Cuts at: 898 3791 898
Size: 2893 1381
SfcI C'TryA_G
Cuts at: 33 864 1317 2002 2995 3186 33
Size: 831 453 685 993 191 1121
Fragments arranged by size:
1121 993 831 685 453 191
SfoI GGC'GCC
Cuts at: 1706 1954 1706
Size: 248 4026
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4274
SmaI CCC'GGG
Cuts at: 1296 1296
Size: 4274
SmlI C'TyrA_G
Cuts at: 1429 2344 2836 3098 1429
Size: 915 492 262 2605
Fragments arranged by size:
2605 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4274
SphI G_CATG'C
Cuts at: 627 1327 1827 627
Size: 700 500 3074
Fragments arranged by size:
3074 700 500
SspI AAT'ATT
Cuts at: 976 976
Size: 4274
StuI AGG'CCT
Cuts at: 1227 1227
Size: 4274
StyI C'CwwG_G
Cuts at: 576 584 711 783 825 1408 1762 576
Size: 8 127 72 42 583 354 3088
Fragments arranged by size:
3088 583 354 127 72 42 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 357 1857 1990 2449 2658 357
Size: 1500 133 459 209 1973
Fragments arranged by size:
1973 1500 459 209 133
TatI w'GTAC_w
Cuts at: 630 896 3789 630
Size: 266 2893 1115
Fragments arranged by size:
2893 1115 266
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4242
Tth111I GACn'n_nGTC
Cuts at: 947 1163 2071 947
Size: 216 908 3150
Fragments arranged by size:
3150 908 216
XbaI T'CTAG_A
Cuts at: 1305 1305
Size: 4274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 934 1249 1769 934
Size: 315 520 3439
Fragments arranged by size:
3439 520 315
XmaI C'CCGG_G
Cuts at: 1294 1294
Size: 4274
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HpaI KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsaXI BsiWI BsmI BsmBI BsrGI BssHII Bsu36I ClaI
CspCI FseI FspAI HindIII MfeI NheI NotI NruI
PacI PflMI PmlI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI XhoI
XmnI ZraI