Restriction map for
pF1KB6647
(Circular) (Six-base) MAPSORT of: pF1KB6647.seq Check: 0 from: 1 to: 4325
pF1KB6647 4325 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 458 1363 458
Size: 905 3420
Acc65I G'GTAC_C
Cuts at: 1341 1341
Size: 4325
AclI AA'CG_TT
Cuts at: 4165 4165
Size: 4325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 720 2150 2582 3329 3597 235
Size: 485 1430 432 747 268 963
Fragments arranged by size:
1430 963 747 485 432 268
AflIII A'CryG_T
Cuts at: 535 1677 2781 3758 535
Size: 1142 1104 977 1102
Fragments arranged by size:
1142 1104 1102 977
AgeI A'CCGG_T
Cuts at: 2713 2713
Size: 4325
AlwNI CAG_nnn'CTG
Cuts at: 252 1102 1630 3197 252
Size: 850 528 1567 1380
Fragments arranged by size:
1567 1380 850 528
ApaI G_GGCC'C
Cuts at: 231 1037 231
Size: 806 3519
ApaLI G'TGCA_C
Cuts at: 3095 3095
Size: 4325
ApoI r'AATT_y
Cuts at: 1329 3678 3934 1329
Size: 2349 256 1720
Fragments arranged by size:
2349 1720 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4325
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
AvaI C'yCGr_G
Cuts at: 698 1345 698
Size: 647 3678
BamHI G'GATC_C
Cuts at: 377 1145 1350 377
Size: 768 205 3352
Fragments arranged by size:
3352 768 205
BanI G'GyrC_C
Cuts at: 401 600 1341 1755 2003 2038 401
Size: 199 741 414 248 35 2688
Fragments arranged by size:
2688 741 414 248 199 35
BanII G_rGCy'C
Cuts at: 231 662 1037 1339 2369 231
Size: 431 375 302 1030 2187
Fragments arranged by size:
2187 1030 431 375 302
BbeI G_GCGC'C
Cuts at: 1759 2007 1759
Size: 248 4077
BciVI GTATCCnnnnn_n'
Cuts at: 2223 2984 4325 2223
Size: 761 1341 2223
Fragments arranged by size:
2223 1341 761
BclI T'GATC_A
Cuts at: 1845 1845
Size: 4325
BglII A'GATC_T
Cuts at: 1840 3877 1840
Size: 2037 2288
BlpI GC'TnA_GC
Cuts at: 1437 1437
Size: 4325
Bme1580I G_kGCm'C
Cuts at: 231 938 1037 1950 2043 3099 231
Size: 707 99 913 93 1056 1457
Fragments arranged by size:
1457 1056 913 707 99 93
BmrI ACTGGGnnnn_n'
Cuts at: 1537 1618 1953 4097 1537
Size: 81 335 2144 1765
Fragments arranged by size:
2144 1765 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 832 1331 832
Size: 499 3826
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1501 2380 2872 3170 1501
Size: 879 492 298 2656
Fragments arranged by size:
2656 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 424 4029 424
Size: 3605 720
BsaAI yAC'GTr
Cuts at: 1678 1678
Size: 4325
BsaBI GATnn'nnATC
Cuts at: 6 1839 3876 6
Size: 1833 2037 455
Fragments arranged by size:
2037 1833 455
BsaHI Gr'CG_yC
Cuts at: 1756 2004 2706 4202 1756
Size: 248 702 1496 1879
Fragments arranged by size:
1879 1496 702 248
BsaWI w'CCGG_w
Cuts at: 398 1514 2035 2713 2987 3134 3545 398
Size: 1116 521 678 274 147 411 1178
Fragments arranged by size:
1178 1116 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 122 152 421 451 608 638 1304 1334 122
Size: 30 269 30 157 30 666 30 3113
Fragments arranged by size:
3113 666 269 157 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 326 719 829 896 1096 1229 1234 1267 1270
Size: 393 110 67 200 133 5 33 3
Cuts at: 1270 326
Size: 3381
Fragments arranged by size:
3381 393 200 133 110 67 33 5
3
BseYI C'CCAG_C
Cuts at: 245 693 896 952 1119 1750 2508 3085 3581
Size: 448 203 56 167 631 758 577 496
Cuts at: 3581 245
Size: 989
Fragments arranged by size:
989 758 631 577 496 448 203 167
56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1283 1283
Size: 4325
BsiEI CG_ry'CG
Cuts at: 104 1867 1913 3121 104
Size: 1763 46 1208 1308
Fragments arranged by size:
1763 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 177 1339 2117 2307 3099 177
Size: 1162 778 190 792 1403
Fragments arranged by size:
1403 1162 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 177 231 662 938 1037 1339 1950 2043 2117
Size: 54 431 276 99 302 611 93 74
Cuts at: 2117 2307 2369 3099 177
Size: 190 62 730 1403
Fragments arranged by size:
1403 730 611 431 302 276 190 99
93 74 62 54
BspEI T'CCGG_A
Cuts at: 3545 3545
Size: 4325
BspHI T'CATG_A
Cuts at: 3501 3501
Size: 4325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1375 1589 1891 2272 1375
Size: 214 302 381 3428
Fragments arranged by size:
3428 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2617 2671 4155 4320 50
Size: 2567 54 1484 165 55
Fragments arranged by size:
2567 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 416 2237 416
Size: 1821 2504
BsrFI r'CCGG_y
Cuts at: 2323 2504 2713 2323
Size: 181 209 3935
Fragments arranged by size:
3935 209 181
BssSI C'ACGA_G
Cuts at: 335 2596 2954 335
Size: 2261 358 1706
Fragments arranged by size:
2261 1706 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1702 1702
Size: 4325
BstBI TT'CG_AA
Cuts at: 2686 2686
Size: 4325
BstEII G'GTnAC_C
Cuts at: 687 687
Size: 4325
BstXI CCAn_nnnn'nTGG
Cuts at: 469 528 469
Size: 59 4266
BstYI r'GATC_y
Cuts at: 1 377 708 1145 1350 1817 1840 2175 2421
Size: 376 331 437 205 467 23 335 246
Cuts at: 2421 3433 3519 3531 3877 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 437 376 346 335 331
246 205 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 986 1289 986
Size: 303 4022
BtgI C'CryG_G
Cuts at: 107 1029 107
Size: 922 3403
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2275 2456 2619 2275
Size: 181 163 3981
Fragments arranged by size:
3981 181 163
BtsI GCAGTG_nn'
Cuts at: 667 1568 667
Size: 901 3424
DraI TTT'AAA
Cuts at: 1324 1324
Size: 4325
DrdI GACnn_nn'nnGTC
Cuts at: 540 2031 2889 540
Size: 1491 858 1976
Fragments arranged by size:
1976 1491 858
EaeI y'GGCC_r
Cuts at: 1013 1910 2084 2475 2502 3847 1013
Size: 897 174 391 27 1345 1491
Fragments arranged by size:
1491 1345 897 391 174 27
EagI C'GGCC_G
Cuts at: 1910 1910
Size: 4325
EarI CTCTTCn'nnn_
Cuts at: 364 667 1244 2348 2558 364
Size: 303 577 1104 210 2131
Fragments arranged by size:
2131 1104 577 303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2533 2843 2989 4135 2533
Size: 310 146 1146 2723
Fragments arranged by size:
2723 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1337 1337
Size: 4325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 720 832 1331 2150 2582 3329 3597 235
Size: 485 112 499 819 432 747 268 963
Fragments arranged by size:
963 819 747 499 485 432 268 112
EcoNI CCTnn'n_nnAGG
Cuts at: 653 977 1292 653
Size: 324 315 3686
Fragments arranged by size:
3686 324 315
EcoO109I rG'GnC_Cy
Cuts at: 227 228 294 316 982 1034 1230 1315 1464
Size: 1 66 22 666 52 196 85 149
Cuts at: 1464 227
Size: 3088
Fragments arranged by size:
3088 666 196 149 85 66 52 22
1
EcoRI G'AATT_C
Cuts at: 1329 1329
Size: 4325
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2344 2344
Size: 4325
FspI TGC'GCA
Cuts at: 769 2106 769
Size: 1337 2988
HaeII r_GCGC'y
Cuts at: 1759 2007 3029 4008 1759
Size: 248 1022 979 2076
Fragments arranged by size:
2076 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 421 440 453 472 481 513 969 1001 2167
Size: 19 13 19 9 32 456 32 1166
Cuts at: 2167 2197 2199 2229 421
Size: 30 2 30 2517
Fragments arranged by size:
2517 1166 456 32 32 30 30 19
19 13 9 2
HincII GTy'rAC
Cuts at: 1364 1364
Size: 4325
HindIII A'AGCT_T
Cuts at: 284 284
Size: 4325
KasI G'GCGC_C
Cuts at: 1755 2003 1755
Size: 248 4077
KpnI G_GTAC'C
Cuts at: 1345 1345
Size: 4325
MfeI C'AATT_G
Cuts at: 823 823
Size: 4325
MluI A'CGCG_T
Cuts at: 3758 3758
Size: 4325
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 108 464 656 1259 2996 3180 108
Size: 356 192 603 1737 184 1253
Fragments arranged by size:
1737 1253 603 356 192 184
MscI TGG'CCA
Cuts at: 1015 2086 1015
Size: 1071 3254
MslI CAynn'nnrTG
Cuts at: 584 2314 2441 584
Size: 1730 127 2468
Fragments arranged by size:
2468 1730 127
MspA1I CmG'CkG
Cuts at: 249 1007 1436 1750 2110 3123 3368 249
Size: 758 429 314 360 1013 245 1206
Fragments arranged by size:
1206 1013 758 429 360 314 245
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 4325
NarI GG'CG_CC
Cuts at: 1756 2004 1756
Size: 248 4077
NcoI C'CATG_G
Cuts at: 107 107
Size: 4325
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 4325
NspI r_CATG'y
Cuts at: 539 1378 1878 2785 539
Size: 839 500 907 2079
Fragments arranged by size:
2079 907 839 500
PasI CC'CwG_GG
Cuts at: 306 320 1140 306
Size: 14 820 3491
Fragments arranged by size:
3491 820 14
PciI A'CATG_T
Cuts at: 535 2781 535
Size: 2246 2079
PflMI CCAn_nnn'nTGG
Cuts at: 252 828 844 252
Size: 576 16 3733
Fragments arranged by size:
3733 576 16
PfoI T'CCnGG_A
Cuts at: 380 380
Size: 4325
PmeI GTTT'AAAC
Cuts at: 1324 1324
Size: 4325
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3464 3496 3464
Size: 32 4293
PpuMI rG'GwC_Cy
Cuts at: 294 294
Size: 4325
PspOMI G'GGCC_C
Cuts at: 227 1033 227
Size: 806 3519
PstI C_TGCA'G
Cuts at: 564 674 1098 1372 2057 564
Size: 110 424 274 685 2832
Fragments arranged by size:
2832 685 424 274 110
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4325
PvuII CAG'CTG
Cuts at: 1007 1750 2110 1007
Size: 743 360 3222
Fragments arranged by size:
3222 743 360
RsrII CG'GwC_CG
Cuts at: 2520 2520
Size: 4325
SacI G_AGCT'C
Cuts at: 1339 1339
Size: 4325
SalI G'TCGA_C
Cuts at: 1362 1362
Size: 4325
SapI GCTCTTCn'nnn_
Cuts at: 667 2348 2558 667
Size: 1681 210 2434
Fragments arranged by size:
2434 1681 210
SbfI CC_TGCA'GG
Cuts at: 564 1372 564
Size: 808 3517
ScaI AGT'ACT
Cuts at: 1308 3842 1308
Size: 2534 1791
SfcI C'TryA_G
Cuts at: 33 560 670 729 1002 1094 1368 2053 3046
Size: 527 110 59 273 92 274 685 993
Cuts at: 3046 3237 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 527 274 273 191 110
92 59
SfoI GGC'GCC
Cuts at: 1757 2005 1757
Size: 248 4077
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4325
SmaI CCC'GGG
Cuts at: 700 1347 700
Size: 647 3678
SmlI C'TyrA_G
Cuts at: 1480 2395 2887 3149 1480
Size: 915 492 262 2656
Fragments arranged by size:
2656 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4325
SphI G_CATG'C
Cuts at: 1378 1878 1378
Size: 500 3825
StuI AGG'CCT
Cuts at: 746 1157 746
Size: 411 3914
StyI C'CwwG_G
Cuts at: 107 1459 1813 107
Size: 1352 354 2619
Fragments arranged by size:
2619 1352 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 126 1033 1908 2041 2500 2709 126
Size: 907 875 133 459 209 1742
Fragments arranged by size:
1742 907 875 459 209 133
TatI w'GTAC_w
Cuts at: 1306 3840 1306
Size: 2534 1791
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 122 154 608 640 3842 3874 122
Size: 32 454 32 3202 32 573
Fragments arranged by size:
3202 573 454 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 435 2122 435
Size: 1687 2638
XbaI T'CTAG_A
Cuts at: 1356 1356
Size: 4325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1820 1820
Size: 4325
XmaI C'CCGG_G
Cuts at: 698 1345 698
Size: 647 3678
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI