Restriction map for
pF1KB6648
(Circular) (Six-base) MAPSORT of: pF1KB6648.seq Check: 8978 from: 1 to: 4448
pF1KB6648 4448 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1486 1486
Size: 4448
Acc65I G'GTAC_C
Cuts at: 1464 1464
Size: 4448
AclI AA'CG_TT
Cuts at: 4288 4288
Size: 4448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 499 1051 1366 2273 2705 3452 3720 290
Size: 209 552 315 907 432 747 268 1018
Fragments arranged by size:
1018 907 747 552 432 315 268 209
AflIII A'CryG_T
Cuts at: 653 1800 2904 3881 653
Size: 1147 1104 977 1220
Fragments arranged by size:
1220 1147 1104 977
AgeI A'CCGG_T
Cuts at: 2836 2836
Size: 4448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1177 1178 1209 1210 1177
Size: 1 31 1 4415
Fragments arranged by size:
4415 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 247 1431 1753 3320 247
Size: 1184 322 1567 1375
Fragments arranged by size:
1567 1375 1184 322
ApaLI G'TGCA_C
Cuts at: 3218 3218
Size: 4448
ApoI r'AATT_y
Cuts at: 276 802 812 899 1037 1302 1452 3801 4057
Size: 526 10 87 138 265 150 2349 256
Cuts at: 4057 276
Size: 667
Fragments arranged by size:
2349 667 526 265 256 150 138 87
10
AseI AT'TA_AT
Cuts at: 20 742 20
Size: 722 3726
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
AvaI C'yCGr_G
Cuts at: 116 1468 116
Size: 1352 3096
BamHI G'GATC_C
Cuts at: 1473 1473
Size: 4448
BanI G'GyrC_C
Cuts at: 1464 1878 2126 2161 1464
Size: 414 248 35 3751
Fragments arranged by size:
3751 414 248 35
BanII G_rGCy'C
Cuts at: 1065 1380 1462 2492 1065
Size: 315 82 1030 3021
Fragments arranged by size:
3021 1030 315 82
BbeI G_GCGC'C
Cuts at: 1882 2130 1882
Size: 248 4200
BbvCI CC'TCA_GC
Cuts at: 122 122
Size: 4448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4414
BciVI GTATCCnnnnn_n'
Cuts at: 2346 3107 4448 2346
Size: 761 1341 2346
Fragments arranged by size:
2346 1341 761
BclI T'GATC_A
Cuts at: 1968 1968
Size: 4448
BglII A'GATC_T
Cuts at: 1963 4000 1963
Size: 2037 2411
BlpI GC'TnA_GC
Cuts at: 1560 1560
Size: 4448
Bme1580I G_kGCm'C
Cuts at: 2073 2166 3222 2073
Size: 93 1056 3299
Fragments arranged by size:
3299 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1660 1741 2076 4220 1660
Size: 81 335 2144 1888
Fragments arranged by size:
2144 1888 335 81
BmtI G_CTAG'C
Cuts at: 595 595
Size: 4448
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 4448
Bpu10I CC'TnA_GC
Cuts at: 122 122
Size: 4448
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 925 976 1364 1624 2503 2995 3293 925
Size: 51 388 260 879 492 298 2080
Fragments arranged by size:
2080 879 492 388 298 260 51
BsaI GGTCTCn'nnnn_
Cuts at: 4152 4152
Size: 4448
BsaAI yAC'GTr
Cuts at: 1801 1801
Size: 4448
BsaBI GATnn'nnATC
Cuts at: 6 878 1962 3999 6
Size: 872 1084 2037 455
Fragments arranged by size:
2037 1084 872 455
BsaHI Gr'CG_yC
Cuts at: 1879 2127 2829 4325 1879
Size: 248 702 1496 2002
Fragments arranged by size:
2002 1496 702 248
BsaWI w'CCGG_w
Cuts at: 182 335 1637 2158 2836 3110 3257 3668 182
Size: 153 1302 521 678 274 147 411 962
Fragments arranged by size:
1302 962 678 521 411 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 177 207 217 247 177
Size: 30 10 30 4378
Fragments arranged by size:
4378 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1308 1308
Size: 4448
BseYI C'CCAG_C
Cuts at: 781 1367 1873 2631 3208 3704 781
Size: 586 506 758 577 496 1525
Fragments arranged by size:
1525 758 586 577 506 496
BsiEI CG_ry'CG
Cuts at: 104 168 1990 2036 3244 104
Size: 64 1822 46 1208 1308
Fragments arranged by size:
1822 1308 1208 64 46
BsiHKAI G_wGCw'C
Cuts at: 1065 1462 2240 2430 3222 1065
Size: 397 778 190 792 2291
Fragments arranged by size:
2291 792 778 397 190
BsmI GAATG_Cn'
Cuts at: 490 490
Size: 4448
Bsp1286I G_dGCh'C
Cuts at: 1065 1380 1462 2073 2166 2240 2430 2492 3222
Size: 315 82 611 93 74 190 62 730
Cuts at: 3222 1065
Size: 2291
Fragments arranged by size:
2291 730 611 315 190 93 82 74
62
BspEI T'CCGG_A
Cuts at: 182 3668 182
Size: 3486 962
BspHI T'CATG_A
Cuts at: 1289 3624 1289
Size: 2335 2113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1498 1712 2014 2395 1498
Size: 214 302 381 3551
Fragments arranged by size:
3551 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2740 2794 4278 4443 50
Size: 2690 54 1484 165 55
Fragments arranged by size:
2690 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2360 2360
Size: 4448
BsrFI r'CCGG_y
Cuts at: 2446 2627 2836 2446
Size: 181 209 4058
Fragments arranged by size:
4058 209 181
BsrGI T'GTAC_A
Cuts at: 140 353 140
Size: 213 4235
BssSI C'ACGA_G
Cuts at: 615 2719 3077 615
Size: 2104 358 1986
Fragments arranged by size:
2104 1986 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1825 1825
Size: 4448
BstBI TT'CG_AA
Cuts at: 2809 2809
Size: 4448
BstYI r'GATC_y
Cuts at: 1 179 384 528 1427 1473 1940 1963 2298
Size: 178 205 144 899 46 467 23 335
Cuts at: 2298 2544 3556 3642 3654 4000 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 899 467 449 346 335 246 205
178 144 86 46 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2398 2579 2742 2398
Size: 181 163 4104
Fragments arranged by size:
4104 181 163
BtsI GCAGTG_nn'
Cuts at: 1691 1691
Size: 4448
DraI TTT'AAA
Cuts at: 1447 1447
Size: 4448
DrdI GACnn_nn'nnGTC
Cuts at: 2154 3012 2154
Size: 858 3590
EaeI y'GGCC_r
Cuts at: 165 2033 2207 2598 2625 3970 165
Size: 1868 174 391 27 1345 643
Fragments arranged by size:
1868 1345 643 391 174 27
EagI C'GGCC_G
Cuts at: 165 2033 165
Size: 1868 2580
EarI CTCTTCn'nnn_
Cuts at: 184 431 957 2471 2681 184
Size: 247 526 1514 210 1951
Fragments arranged by size:
1951 1514 526 247 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2656 2966 3112 4258 2656
Size: 310 146 1146 2846
Fragments arranged by size:
2846 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1063 1460 1063
Size: 397 4051
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 499 1051 1366 2273 2705 3452 3720 290
Size: 209 552 315 907 432 747 268 1018
Fragments arranged by size:
1018 907 747 552 432 315 268 209
EcoO109I rG'GnC_Cy
Cuts at: 1341 1587 1341
Size: 246 4202
EcoRI G'AATT_C
Cuts at: 812 1302 1452 812
Size: 490 150 3808
Fragments arranged by size:
3808 490 150
EcoRV GAT'ATC
Cuts at: 702 702
Size: 4448
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 919 2467 919
Size: 1548 2900
FspI TGC'GCA
Cuts at: 2229 2229
Size: 4448
HaeII r_GCGC'y
Cuts at: 1882 2130 3152 4131 1882
Size: 248 1022 979 2199
Fragments arranged by size:
2199 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 217 249 691 723 2290 2320 2322 2352 217
Size: 32 442 32 1567 30 2 30 2313
Fragments arranged by size:
2313 1567 442 32 32 30 30 2
HincII GTy'rAC
Cuts at: 467 1487 467
Size: 1020 3428
HindIII A'AGCT_T
Cuts at: 574 574
Size: 4448
KasI G'GCGC_C
Cuts at: 1878 2126 1878
Size: 248 4200
KpnI G_GTAC'C
Cuts at: 1468 1468
Size: 4448
MluI A'CGCG_T
Cuts at: 3881 3881
Size: 4448
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3119 3303 3119
Size: 184 4264
MscI TGG'CCA
Cuts at: 2209 2209
Size: 4448
MslI CAynn'nnrTG
Cuts at: 361 1265 2437 2564 361
Size: 904 1172 127 2245
Fragments arranged by size:
2245 1172 904 127
MspA1I CmG'CkG
Cuts at: 170 1371 1559 1873 2233 3246 3491 170
Size: 1201 188 314 360 1013 245 1127
Fragments arranged by size:
1201 1127 1013 360 314 245 188
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 4448
NarI GG'CG_CC
Cuts at: 1879 2127 1879
Size: 248 4200
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 4448
NheI G'CTAG_C
Cuts at: 591 591
Size: 4448
NspI r_CATG'y
Cuts at: 657 681 1440 1501 2001 2908 657
Size: 24 759 61 500 907 2197
Fragments arranged by size:
2197 907 759 500 61 24
PciI A'CATG_T
Cuts at: 653 2904 653
Size: 2251 2197
PfoI T'CCnGG_A
Cuts at: 551 551
Size: 4448
PmeI GTTT'AAAC
Cuts at: 1447 1447
Size: 4448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3587 3619 3587
Size: 32 4416
PpuMI rG'GwC_Cy
Cuts at: 1341 1341
Size: 4448
PstI C_TGCA'G
Cuts at: 1495 2180 1495
Size: 685 3763
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4448
PvuII CAG'CTG
Cuts at: 1371 1873 2233 1371
Size: 502 360 3586
Fragments arranged by size:
3586 502 360
RsrII CG'GwC_CG
Cuts at: 2643 2643
Size: 4448
SacI G_AGCT'C
Cuts at: 1065 1462 1065
Size: 397 4051
SalI G'TCGA_C
Cuts at: 1485 1485
Size: 4448
SapI GCTCTTCn'nnn_
Cuts at: 2471 2681 2471
Size: 210 4238
SbfI CC_TGCA'GG
Cuts at: 1495 1495
Size: 4448
ScaI AGT'ACT
Cuts at: 3965 3965
Size: 4448
SfcI C'TryA_G
Cuts at: 33 1170 1491 2176 3169 3360 33
Size: 1137 321 685 993 191 1121
Fragments arranged by size:
1137 1121 993 685 321 191
SfoI GGC'GCC
Cuts at: 1880 2128 1880
Size: 248 4200
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4448
SmaI CCC'GGG
Cuts at: 118 1470 118
Size: 1352 3096
SmlI C'TyrA_G
Cuts at: 904 955 1379 1603 2518 3010 3272 904
Size: 51 424 224 915 492 262 2080
Fragments arranged by size:
2080 915 492 424 262 224 51
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4448
SphI G_CATG'C
Cuts at: 1440 1501 2001 1440
Size: 61 500 3887
Fragments arranged by size:
3887 500 61
SspI AAT'ATT
Cuts at: 370 602 370
Size: 232 4216
StyI C'CwwG_G
Cuts at: 413 1582 1936 413
Size: 1169 354 2925
Fragments arranged by size:
2925 1169 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2031 2164 2623 2832 2031
Size: 133 459 209 3647
Fragments arranged by size:
3647 459 209 133
TatI w'GTAC_w
Cuts at: 140 353 3963 140
Size: 213 3610 625
Fragments arranged by size:
3610 625 213
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 177 209 3965 3997 177
Size: 32 3756 32 628
Fragments arranged by size:
3756 628 32 32
Tth111I GACn'n_nGTC
Cuts at: 2245 2245
Size: 4448
XbaI T'CTAG_A
Cuts at: 1479 1479
Size: 4448
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 549 1943 549
Size: 1394 3054
XmaI C'CCGG_G
Cuts at: 116 1468 116
Size: 1352 3096
XmnI GAAnn'nnTTC
Cuts at: 899 899
Size: 4448
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NspI PciI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI ApaI
AscI AvrII BaeI BbsI BfrBI BglI BmgBI BpmI
BsgI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NdeI NotI NruI NsiI PacI PasI
PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI