Restriction map for
pF1KB6655
(Circular) (Six-base) MAPSORT of: pF1KB6655.seq Check: 2826 from: 1 to: 4382
pF1KB6655 4382 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 170 170
Size: 4382
AccI GT'mk_AC
Cuts at: 1420 1420
Size: 4382
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 4222 4222
Size: 4382
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 388 1319 2207 2639 3386 3654 388
Size: 931 888 432 747 268 1116
Fragments arranged by size:
1116 931 888 747 432 268
AflIII A'CryG_T
Cuts at: 1734 2838 3815 1734
Size: 1104 977 2301
Fragments arranged by size:
2301 1104 977
AgeI A'CCGG_T
Cuts at: 1109 2770 1109
Size: 1661 2721
AleI CACnn'nnGTG
Cuts at: 358 358
Size: 4382
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 844 876 1126 1158 844
Size: 32 250 32 4068
Fragments arranged by size:
4068 250 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1687 3254 1687
Size: 1567 2815
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 792 1386 3735 3991 792
Size: 594 2349 256 1183
Fragments arranged by size:
2349 1183 594 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BamHI G'GATC_C
Cuts at: 1407 1407
Size: 4382
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 1396 2426 1396
Size: 1030 3352
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BciVI GTATCCnnnnn_n'
Cuts at: 1272 2280 3041 4382 1272
Size: 1008 761 1341 1272
Fragments arranged by size:
1341 1272 1008 761
BclI T'GATC_A
Cuts at: 830 938 1902 830
Size: 108 964 3310
Fragments arranged by size:
3310 964 108
BfrBI ATG'CAT
Cuts at: 655 671 655
Size: 16 4366
BglI GCCn_nnn'nGGC
Cuts at: 1234 1234
Size: 4382
BglII A'GATC_T
Cuts at: 980 1897 3934 980
Size: 917 2037 1428
Fragments arranged by size:
2037 1428 917
BlpI GC'TnA_GC
Cuts at: 1494 1494
Size: 4382
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 172 1594 1675 2010 4154 172
Size: 1422 81 335 2144 400
Fragments arranged by size:
2144 1422 400 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 168 504 2749 168
Size: 336 2245 1801
Fragments arranged by size:
2245 1801 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 1153 1312 1378 163
Size: 990 159 66 3167
Fragments arranged by size:
3167 990 159 66
Bpu10I CC'TnA_GC
Cuts at: 206 206
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 316 703 1168 1177 4086 316
Size: 387 465 9 2909 612
Fragments arranged by size:
2909 612 465 387 9
BsaAI yAC'GTr
Cuts at: 276 1735 276
Size: 1459 2923
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 167 1813 2061 2763 4259 167
Size: 1646 248 702 1496 290
Fragments arranged by size:
1646 1496 702 290 248
BsaWI w'CCGG_w
Cuts at: 1109 1571 2092 2770 3044 3191 3602 1109
Size: 462 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 462 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 844 874 1126 1156 844
Size: 30 252 30 4070
Fragments arranged by size:
4070 252 30 30
BseYI C'CCAG_C
Cuts at: 992 1807 2565 3142 3638 992
Size: 815 758 577 496 1736
Fragments arranged by size:
1736 815 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1396 2174 2364 3156 1396
Size: 778 190 792 2622
Fragments arranged by size:
2622 792 778 190
BsmI GAATG_Cn'
Cuts at: 655 655
Size: 4382
BsmBI CGTCTCn'nnnn_
Cuts at: 531 531
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 1396 2007 2100 2174 2364 2426 3156 1396
Size: 611 93 74 190 62 730 2622
Fragments arranged by size:
2622 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 3558 3558
Size: 4382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 233 1432 1646 1948 2329 233
Size: 1199 214 302 381 2286
Fragments arranged by size:
2286 1199 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 303 2294 303
Size: 1991 2391
BsrFI r'CCGG_y
Cuts at: 1109 2380 2561 2770 1109
Size: 1271 181 209 2721
Fragments arranged by size:
2721 1271 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstEII G'GTnAC_C
Cuts at: 1105 1105
Size: 4382
BstXI CCAn_nnnn'nTGG
Cuts at: 360 1215 360
Size: 855 3527
BstYI r'GATC_y
Cuts at: 1 980 1094 1407 1874 1897 2232 2478 3490
Size: 979 114 313 467 23 335 246 1012
Cuts at: 3490 3576 3588 3934 1
Size: 86 12 346 449
Fragments arranged by size:
1012 979 467 449 346 335 313 246
114 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 787 787
Size: 4382
BtgI C'CryG_G
Cuts at: 107 155 107
Size: 48 4334
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 740 1625 740
Size: 885 3497
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DraIII CAC_nnn'GTG
Cuts at: 804 804
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 110 182 1217 1967 2141 2532 2559 3904 110
Size: 72 1035 750 174 391 27 1345 588
Fragments arranged by size:
1345 1035 750 588 391 174 72 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 694 1295 1316 2405 2615 694
Size: 601 21 1089 210 2461
Fragments arranged by size:
2461 1089 601 210 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 388 1153 1312 1319 1378 2207 2639 3386
Size: 225 765 159 7 59 829 432 747
Cuts at: 3386 3654 163
Size: 268 891
Fragments arranged by size:
891 829 765 747 432 268 225 159
59 7
EcoO109I rG'GnC_Cy
Cuts at: 483 783 1521 483
Size: 300 738 3344
Fragments arranged by size:
3344 738 300
EcoRI G'AATT_C
Cuts at: 792 1386 792
Size: 594 3788
EcoRV GAT'ATC
Cuts at: 345 885 345
Size: 540 3842
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1383 2224 2254 2256 2286 1351
Size: 32 841 30 2 30 3447
Fragments arranged by size:
3447 841 32 30 30 2
HincII GTy'rAC
Cuts at: 1155 1421 1155
Size: 266 4116
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 112 184 2143 112
Size: 72 1959 2351
Fragments arranged by size:
2351 1959 72
MslI CAynn'nnrTG
Cuts at: 358 1054 2371 2498 358
Size: 696 1317 127 2242
Fragments arranged by size:
2242 1317 696 127
MspA1I CmG'CkG
Cuts at: 936 996 1493 1807 2167 3180 3425 936
Size: 60 497 314 360 1013 245 1893
Fragments arranged by size:
1893 1013 497 360 314 245 60
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 155 107
Size: 48 4334
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NsiI A_TGCA'T
Cuts at: 657 673 657
Size: 16 4366
NspI r_CATG'y
Cuts at: 675 1064 1285 1435 1935 2842 675
Size: 389 221 150 500 907 2215
Fragments arranged by size:
2215 907 500 389 221 150
PciI A'CATG_T
Cuts at: 2838 2838
Size: 4382
PflMI CCAn_nnn'nTGG
Cuts at: 804 804
Size: 4382
PfoI T'CCnGG_A
Cuts at: 140 490 140
Size: 350 4032
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 844 876 1126 1158 3521 3553 844
Size: 32 250 32 2363 32 1673
Fragments arranged by size:
2363 1673 250 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 483 783 483
Size: 300 4082
PshAI GACnn'nnGTC
Cuts at: 136 715 136
Size: 579 3803
PstI C_TGCA'G
Cuts at: 1429 2114 1429
Size: 685 3697
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 996 1807 2167 996
Size: 811 360 3211
Fragments arranged by size:
3211 811 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 1316 2405 2615 1316
Size: 1089 210 3083
Fragments arranged by size:
3083 1089 210
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 1425 2110 3103 3294 33
Size: 1392 685 993 191 1121
Fragments arranged by size:
1392 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 1537 2452 2944 3206 1537
Size: 915 492 262 2713
Fragments arranged by size:
2713 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 155 1516 1870 107
Size: 48 1361 354 2619
Fragments arranged by size:
2619 1361 354 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 355 742 1965 2098 2557 2766 355
Size: 387 1223 133 459 209 1971
Fragments arranged by size:
1971 1223 459 387 209 133
TatI w'GTAC_w
Cuts at: 3897 3897
Size: 4382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 486 518 3899 3931 355
Size: 32 99 32 3381 32 806
Fragments arranged by size:
3381 806 99 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1008 1877 1008
Size: 869 3513
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
ZraI GAC'GTC
Cuts at: 168 168
Size: 4382
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BmgBI BmtI BseRI BsgI
BsiWI BsrGI BssHII BstZ17I ClaI CspCI EcoNI FseI
FspAI HindIII HpaI MfeI NdeI NheI NotI NruI
PacI PasI PmlI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI