Restriction map for
pF1KB6657
(Circular) (Six-base) MAPSORT of: pF1KB6657.seq Check: 2553 from: 1 to: 4382
pF1KB6657 4382 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 373 757 1283 1420 373
Size: 384 526 137 3335
Fragments arranged by size:
3335 526 384 137
Acc65I G'GTAC_C
Cuts at: 1398 1398
Size: 4382
AclI AA'CG_TT
Cuts at: 1038 4222 1038
Size: 3184 1198
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 2207 2639 3386 3654 932
Size: 1275 432 747 268 1660
Fragments arranged by size:
1660 1275 747 432 268
AflII C'TTAA_G
Cuts at: 976 976
Size: 4382
AflIII A'CryG_T
Cuts at: 255 1734 2838 3815 255
Size: 1479 1104 977 822
Fragments arranged by size:
1479 1104 977 822
AgeI A'CCGG_T
Cuts at: 2770 2770
Size: 4382
AlwNI CAG_nnn'CTG
Cuts at: 291 910 1179 1687 3254 291
Size: 619 269 508 1567 1419
Fragments arranged by size:
1567 1419 619 508 269
ApaLI G'TGCA_C
Cuts at: 3152 3152
Size: 4382
ApoI r'AATT_y
Cuts at: 715 1386 3735 3991 715
Size: 671 2349 256 1106
Fragments arranged by size:
2349 1106 671 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4382
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
AvaI C'yCGr_G
Cuts at: 1402 1402
Size: 4382
BamHI G'GATC_C
Cuts at: 1080 1407 1080
Size: 327 4055
BanI G'GyrC_C
Cuts at: 1398 1812 2060 2095 1398
Size: 414 248 35 3685
Fragments arranged by size:
3685 414 248 35
BanII G_rGCy'C
Cuts at: 1396 2426 1396
Size: 1030 3352
BbeI G_GCGC'C
Cuts at: 1816 2064 1816
Size: 248 4134
BbsI GAAGACnn'nnnn_
Cuts at: 829 1271 829
Size: 442 3940
BciVI GTATCCnnnnn_n'
Cuts at: 1212 2280 3041 4382 1212
Size: 1068 761 1341 1212
Fragments arranged by size:
1341 1212 1068 761
BclI T'GATC_A
Cuts at: 1902 1902
Size: 4382
BfrBI ATG'CAT
Cuts at: 398 398
Size: 4382
BglII A'GATC_T
Cuts at: 749 1897 3934 749
Size: 1148 2037 1197
Fragments arranged by size:
2037 1197 1148
BlpI GC'TnA_GC
Cuts at: 113 1494 113
Size: 1381 3001
Bme1580I G_kGCm'C
Cuts at: 2007 2100 3156 2007
Size: 93 1056 3233
Fragments arranged by size:
3233 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1594 1675 2010 4154 1594
Size: 81 335 2144 1822
Fragments arranged by size:
2144 1822 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 348 2749 348
Size: 2401 1981
Bpu10I CC'TnA_GC
Cuts at: 705 705
Size: 4382
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1558 2437 2929 3227 1558
Size: 879 492 298 2713
Fragments arranged by size:
2713 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1163 4086 1163
Size: 2923 1459
BsaAI yAC'GTr
Cuts at: 363 1735 363
Size: 1372 3010
BsaBI GATnn'nnATC
Cuts at: 6 1896 3933 6
Size: 1890 2037 455
Fragments arranged by size:
2037 1890 455
BsaHI Gr'CG_yC
Cuts at: 1813 2061 2763 4259 1813
Size: 248 702 1496 1936
Fragments arranged by size:
1936 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1571 2092 2770 3044 3191 3602 1571
Size: 521 678 274 147 411 2351
Fragments arranged by size:
2351 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 834 864 834
Size: 30 4352
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 805 1229 1331 805
Size: 424 102 3856
Fragments arranged by size:
3856 424 102
BseYI C'CCAG_C
Cuts at: 383 1807 2565 3142 3638 383
Size: 1424 758 577 496 1127
Fragments arranged by size:
1424 1127 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 4382
BsiEI CG_ry'CG
Cuts at: 104 1924 1970 3178 104
Size: 1820 46 1208 1308
Fragments arranged by size:
1820 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1396 2174 2364 3156 1396
Size: 778 190 792 2622
Fragments arranged by size:
2622 792 778 190
BsmI GAATG_Cn'
Cuts at: 302 302
Size: 4382
Bsp1286I G_dGCh'C
Cuts at: 1396 2007 2100 2174 2364 2426 3156 1396
Size: 611 93 74 190 62 730 2622
Fragments arranged by size:
2622 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3602 3602
Size: 4382
BspHI T'CATG_A
Cuts at: 1260 3558 1260
Size: 2298 2084
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1432 1646 1948 2329 1432
Size: 214 302 381 3485
Fragments arranged by size:
3485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2674 2728 4212 4377 50
Size: 2624 54 1484 165 55
Fragments arranged by size:
2624 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 400 2294 400
Size: 1894 2488
BsrFI r'CCGG_y
Cuts at: 2380 2561 2770 2380
Size: 181 209 3992
Fragments arranged by size:
3992 209 181
BssSI C'ACGA_G
Cuts at: 2653 3011 2653
Size: 358 4024
BstAPI GCAn_nnn'nTGC
Cuts at: 1759 1759
Size: 4382
BstBI TT'CG_AA
Cuts at: 2743 2743
Size: 4382
BstYI r'GATC_y
Cuts at: 1 446 479 749 1080 1189 1407 1874 1897
Size: 445 33 270 331 109 218 467 23
Cuts at: 1897 2232 2478 3490 3576 3588 3934 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 445 346 335 331 270
246 218 109 86 33 23 12
BstZ17I GTA'TAC
Cuts at: 374 374
Size: 4382
BtgI C'CryG_G
Cuts at: 107 107
Size: 4382
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2332 2513 2676 2332
Size: 181 163 4038
Fragments arranged by size:
4038 181 163
BtsI GCAGTG_nn'
Cuts at: 654 729 930 1625 654
Size: 75 201 695 3411
Fragments arranged by size:
3411 695 201 75
DraI TTT'AAA
Cuts at: 1381 1381
Size: 4382
DrdI GACnn_nn'nnGTC
Cuts at: 2088 2946 2088
Size: 858 3524
EaeI y'GGCC_r
Cuts at: 870 1967 2141 2532 2559 3904 870
Size: 1097 174 391 27 1345 1348
Fragments arranged by size:
1348 1345 1097 391 174 27
EagI C'GGCC_G
Cuts at: 1967 1967
Size: 4382
EarI CTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2590 2900 3046 4192 2590
Size: 310 146 1146 2780
Fragments arranged by size:
2780 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1394 1394
Size: 4382
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 932 2207 2639 3386 3654 932
Size: 1275 432 747 268 1660
Fragments arranged by size:
1660 1275 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 277 277
Size: 4382
EcoO109I rG'GnC_Cy
Cuts at: 1521 1521
Size: 4382
EcoRI G'AATT_C
Cuts at: 1386 1386
Size: 4382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2401 2401
Size: 4382
FspI TGC'GCA
Cuts at: 2163 2163
Size: 4382
HaeII r_GCGC'y
Cuts at: 1816 2064 3086 4065 1816
Size: 248 1022 979 2133
Fragments arranged by size:
2133 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 949 981 2224 2254 2256 2286 553
Size: 32 364 32 1243 30 2 30 2649
Fragments arranged by size:
2649 1243 364 32 32 30 30 2
HincII GTy'rAC
Cuts at: 983 1421 983
Size: 438 3944
KasI G'GCGC_C
Cuts at: 1812 2060 1812
Size: 248 4134
KpnI G_GTAC'C
Cuts at: 1402 1402
Size: 4382
MfeI C'AATT_G
Cuts at: 512 512
Size: 4382
MluI A'CGCG_T
Cuts at: 3815 3815
Size: 4382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3053 3237 3053
Size: 184 4198
MscI TGG'CCA
Cuts at: 872 2143 872
Size: 1271 3111
MslI CAynn'nnrTG
Cuts at: 254 319 527 2371 2498 254
Size: 65 208 1844 127 2138
Fragments arranged by size:
2138 1844 208 127 65
MspA1I CmG'CkG
Cuts at: 1179 1493 1807 2167 3180 3425 1179
Size: 314 314 360 1013 245 2136
Fragments arranged by size:
2136 1013 360 314 314 245
NaeI GCC'GGC
Cuts at: 2563 2563
Size: 4382
NarI GG'CG_CC
Cuts at: 1813 2061 1813
Size: 248 4134
NcoI C'CATG_G
Cuts at: 107 107
Size: 4382
NgoMIV G'CCGG_C
Cuts at: 2561 2561
Size: 4382
NsiI A_TGCA'T
Cuts at: 400 400
Size: 4382
NspI r_CATG'y
Cuts at: 259 402 1435 1935 2842 259
Size: 143 1033 500 907 1799
Fragments arranged by size:
1799 1033 907 500 143
PciI A'CATG_T
Cuts at: 255 2838 255
Size: 2583 1799
PmeI GTTT'AAAC
Cuts at: 1381 1381
Size: 4382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3521 3553 3521
Size: 32 4350
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 149 181 149
Size: 32 4350
PstI C_TGCA'G
Cuts at: 925 1429 2114 925
Size: 504 685 3193
Fragments arranged by size:
3193 685 504
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4382
PvuII CAG'CTG
Cuts at: 1179 1807 2167 1179
Size: 628 360 3394
Fragments arranged by size:
3394 628 360
RsrII CG'GwC_CG
Cuts at: 2577 2577
Size: 4382
SacI G_AGCT'C
Cuts at: 1396 1396
Size: 4382
SalI G'TCGA_C
Cuts at: 1419 1419
Size: 4382
SapI GCTCTTCn'nnn_
Cuts at: 2405 2615 2405
Size: 210 4172
SbfI CC_TGCA'GG
Cuts at: 1429 1429
Size: 4382
ScaI AGT'ACT
Cuts at: 3899 3899
Size: 4382
SfcI C'TryA_G
Cuts at: 33 660 689 758 767 921 1341 1425 2110
Size: 627 29 69 9 154 420 84 685
Cuts at: 2110 3103 3294 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 627 420 191 154 84
69 29 9
SfoI GGC'GCC
Cuts at: 1814 2062 1814
Size: 248 4134
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4382
SmaI CCC'GGG
Cuts at: 1404 1404
Size: 4382
SmlI C'TyrA_G
Cuts at: 976 1537 2452 2944 3206 976
Size: 561 915 492 262 2152
Fragments arranged by size:
2152 915 561 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4382
SphI G_CATG'C
Cuts at: 1435 1935 1435
Size: 500 3882
StyI C'CwwG_G
Cuts at: 107 302 488 1516 1870 107
Size: 195 186 1028 354 2619
Fragments arranged by size:
2619 1028 354 195 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 540 1128 1965 2098 2557 2766 540
Size: 588 837 133 459 209 2156
Fragments arranged by size:
2156 837 588 459 209 133
TatI w'GTAC_w
Cuts at: 3897 3897
Size: 4382
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 986 1018 3899 3931 986
Size: 32 2881 32 1437
Fragments arranged by size:
2881 1437 32 32
Tth111I GACn'n_nGTC
Cuts at: 2179 2179
Size: 4382
XbaI T'CTAG_A
Cuts at: 1413 1413
Size: 4382
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1877 1877
Size: 4382
XmaI C'CCGG_G
Cuts at: 1402 1402
Size: 4382
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
BpmI BsiWI BsmBI BsrGI BssHII BstEII BstXI Bsu36I
ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI