Restriction map for
pF1KB6663
(Circular) (Six-base) MAPSORT of: pF1KB6663.seq Check: 2340 from: 1 to: 4385
pF1KB6663 4385 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 2642 3389 3657 2210
Size: 432 747 268 2938
Fragments arranged by size:
2938 747 432 268
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AhdI GACnn_n'nnGTC
Cuts at: 223 223
Size: 4385
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 191 223 694 726 191
Size: 32 471 32 3850
Fragments arranged by size:
3850 471 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1093 1690 3257 1093
Size: 597 1567 2221
Fragments arranged by size:
2221 1567 597
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 286 1389 3738 3994 286
Size: 1103 2349 256 677
Fragments arranged by size:
2349 1103 677 256
AseI AT'TA_AT
Cuts at: 20 548 20
Size: 528 3857
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1166 1349 1405 1166
Size: 183 56 4146
Fragments arranged by size:
4146 183 56
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 1401 1815 2063 2098 1401
Size: 414 248 35 3688
Fragments arranged by size:
3688 414 248 35
BanII G_rGCy'C
Cuts at: 864 1399 2429 864
Size: 535 1030 2820
Fragments arranged by size:
2820 1030 535
BbeI G_GCGC'C
Cuts at: 1819 2067 1819
Size: 248 4137
BciVI GTATCCnnnnn_n'
Cuts at: 869 1252 2283 3044 4385 869
Size: 383 1031 761 1341 869
Fragments arranged by size:
1341 1031 869 761 383
BclI T'GATC_A
Cuts at: 335 1905 335
Size: 1570 2815
BglI GCCn_nnn'nGGC
Cuts at: 1058 1058
Size: 4385
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 672 1497 672
Size: 825 3560
Bme1580I G_kGCm'C
Cuts at: 2010 2103 3159 2010
Size: 93 1056 3236
Fragments arranged by size:
3236 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 838 1597 1678 2013 4157 838
Size: 759 81 335 2144 1066
Fragments arranged by size:
2144 1066 759 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1387 2752 1387
Size: 1365 3020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 878 193
Size: 685 3700
Bpu10I CC'TnA_GC
Cuts at: 1062 1062
Size: 4385
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 312 1561 2440 2932 3230 312
Size: 1249 879 492 298 1467
Fragments arranged by size:
1467 1249 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4089 4089
Size: 4385
BsaAI yAC'GTr
Cuts at: 156 1738 156
Size: 1582 2803
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1816 2064 2766 4262 1816
Size: 248 702 1496 1939
Fragments arranged by size:
1939 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 191 221 694 724 1254 1284 191
Size: 30 473 30 530 30 3292
Fragments arranged by size:
3292 530 473 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 743 812 1136 1226 743
Size: 69 324 90 3902
Fragments arranged by size:
3902 324 90 69
BseYI C'CCAG_C
Cuts at: 882 1810 2568 3145 3641 882
Size: 928 758 577 496 1626
Fragments arranged by size:
1626 928 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1927 1973 3181 104
Size: 1823 46 1208 1308
Fragments arranged by size:
1823 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
BsmI GAATG_Cn'
Cuts at: 1133 1133
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 864 1399 2010 2103 2177 2367 2429 3159 864
Size: 535 611 93 74 190 62 730 2090
Fragments arranged by size:
2090 730 611 535 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 326 3561 326
Size: 3235 1150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1435 1649 1951 2332 1435
Size: 214 302 381 3488
Fragments arranged by size:
3488 381 302 214
BsrBI CCG'CTC
Cuts at: 50 132 708 2677 2731 4215 4380 50
Size: 82 576 1969 54 1484 165 55
Fragments arranged by size:
1969 1484 576 165 82 55 54
BsrDI GCAATG_nn'
Cuts at: 2297 2297
Size: 4385
BsrFI r'CCGG_y
Cuts at: 2383 2564 2773 2383
Size: 181 209 3995
Fragments arranged by size:
3995 209 181
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1224 1762 1224
Size: 538 3847
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstEII G'GTnAC_C
Cuts at: 1195 1195
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 274 777 274
Size: 503 3882
BstYI r'GATC_y
Cuts at: 1 1183 1410 1877 1900 2235 2481 3493 3579
Size: 1182 227 467 23 335 246 1012 86
Cuts at: 3579 3591 3937 1
Size: 12 346 449
Fragments arranged by size:
1182 1012 467 449 346 335 246 227
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1376 1376
Size: 4385
BtgI C'CryG_G
Cuts at: 107 666 1047 1190 1254 107
Size: 559 381 143 64 3238
Fragments arranged by size:
3238 559 381 143 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2335 2516 2679 2335
Size: 181 163 4041
Fragments arranged by size:
4041 181 163
BtsI GCAGTG_nn'
Cuts at: 1628 1628
Size: 4385
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DraIII CAC_nnn'GTG
Cuts at: 1003 1003
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 965 2091 2949 965
Size: 1126 858 2401
Fragments arranged by size:
2401 1126 858
EaeI y'GGCC_r
Cuts at: 258 1333 1970 2144 2535 2562 3907 258
Size: 1075 637 174 391 27 1345 736
Fragments arranged by size:
1345 1075 736 637 391 174 27
EagI C'GGCC_G
Cuts at: 1970 1970
Size: 4385
EarI CTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 193 878 2210 2642 3389 3657 193
Size: 685 1332 432 747 268 921
Fragments arranged by size:
1332 921 747 685 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 518 809 518
Size: 291 4094
EcoO109I rG'GnC_Cy
Cuts at: 162 510 1171 1204 1303 1366 1524 162
Size: 348 661 33 99 63 158 3023
Fragments arranged by size:
3023 661 348 158 99 63 33
EcoRI G'AATT_C
Cuts at: 286 1389 286
Size: 1103 3282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 2166 2166
Size: 4385
HaeII r_GCGC'y
Cuts at: 473 666 1819 2067 3089 4068 473
Size: 193 1153 248 1022 979 790
Fragments arranged by size:
1153 1022 979 790 248 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 209 241 999 1031 2227 2257 2259 2289 209
Size: 32 758 32 1196 30 2 30 2305
Fragments arranged by size:
2305 1196 758 32 32 30 30 2
HincII GTy'rAC
Cuts at: 228 1424 228
Size: 1196 3189
KasI G'GCGC_C
Cuts at: 1815 2063 1815
Size: 248 4137
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MfeI C'AATT_G
Cuts at: 581 581
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3240 3056
Size: 184 4201
MscI TGG'CCA
Cuts at: 260 2146 260
Size: 1886 2499
MslI CAynn'nnrTG
Cuts at: 235 331 2374 2501 235
Size: 96 2043 127 2119
Fragments arranged by size:
2119 2043 127 96
MspA1I CmG'CkG
Cuts at: 189 735 1299 1496 1810 2170 3183 3428 189
Size: 546 564 197 314 360 1013 245 1146
Fragments arranged by size:
1146 1013 564 546 360 314 245 197
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1816 2064 1816
Size: 248 4137
NcoI C'CATG_G
Cuts at: 107 666 1047 1190 1254 107
Size: 559 381 143 64 3238
Fragments arranged by size:
3238 559 381 143 64
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 240 819 1161 1438 1938 2845 240
Size: 579 342 277 500 907 1780
Fragments arranged by size:
1780 907 579 500 342 277
PasI CC'CwG_GG
Cuts at: 949 949
Size: 4385
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PflMI CCAn_nnn'nTGG
Cuts at: 856 1003 1093 1123 1288 856
Size: 147 90 30 165 3953
Fragments arranged by size:
3953 165 147 90 30
PfoI T'CCnGG_A
Cuts at: 855 1137 855
Size: 282 4103
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 191 223 694 726 3524 3556 191
Size: 32 471 32 2798 32 1020
Fragments arranged by size:
2798 1020 471 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 510 1204 510
Size: 694 3691
PspXI vC'TCGA_Gb
Cuts at: 1349 1349
Size: 4385
PstI C_TGCA'G
Cuts at: 921 1432 2117 921
Size: 511 685 3189
Fragments arranged by size:
3189 685 511
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 735 1810 2170 735
Size: 1075 360 2950
Fragments arranged by size:
2950 1075 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SanDI GG'GwC_CC
Cuts at: 510 510
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SfcI C'TryA_G
Cuts at: 33 917 1428 2113 3106 3297 33
Size: 884 511 685 993 191 1121
Fragments arranged by size:
1121 993 884 685 511 191
SfoI GGC'GCC
Cuts at: 1817 2065 1817
Size: 248 4137
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1407 1407
Size: 4385
SmlI C'TyrA_G
Cuts at: 291 1349 1540 2455 2947 3209 291
Size: 1058 191 915 492 262 1467
Fragments arranged by size:
1467 1058 915 492 262 191
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 819 1161 1438 1938 819
Size: 342 277 500 3266
Fragments arranged by size:
3266 500 342 277
StyI C'CwwG_G
Cuts at: 107 267 653 666 1047 1190 1254 1519 1873
Size: 160 386 13 381 143 64 265 354
Cuts at: 1873 107
Size: 2619
Fragments arranged by size:
2619 386 381 354 265 160 143 64
13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 247 1004 1214 1332 1968 2101 2560 2769 247
Size: 757 210 118 636 133 459 209 1863
Fragments arranged by size:
1863 757 636 459 210 209 133 118
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1254 1286 3902 3934 1254
Size: 32 2616 32 1705
Fragments arranged by size:
2616 1705 32 32
Tth111I GACn'n_nGTC
Cuts at: 2182 2182
Size: 4385
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 153 1054 1261 1880 153
Size: 901 207 619 2658
Fragments arranged by size:
2658 901 619 207
XhoI C'TCGA_G
Cuts at: 1349 1349
Size: 4385
XmaI C'CCGG_G
Cuts at: 1405 1405
Size: 4385
XmnI GAAnn'nnTTC
Cuts at: 203 686 203
Size: 483 3902
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsgI BsiWI BsmBI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI NdeI NheI NotI
NruI NsiI PacI PmlI PshAI PsiI PspOMI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI ZraI