Restriction map for
pF1KB6665
(Circular) (Six-base) MAPSORT of: pF1KB6665.seq Check: 8168 from: 1 to: 4385
pF1KB6665 4385 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1423 1423
Size: 4385
Acc65I G'GTAC_C
Cuts at: 1401 1401
Size: 4385
AclI AA'CG_TT
Cuts at: 4225 4225
Size: 4385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 559 568 1300 2210 2642 3389 3657 559
Size: 9 732 910 432 747 268 1287
Fragments arranged by size:
1287 910 747 732 432 268 9
AflIII A'CryG_T
Cuts at: 1737 2841 3818 1737
Size: 1104 977 2304
Fragments arranged by size:
2304 1104 977
AgeI A'CCGG_T
Cuts at: 2773 2773
Size: 4385
AlwNI CAG_nnn'CTG
Cuts at: 155 735 864 1690 3257 155
Size: 580 129 826 1567 1283
Fragments arranged by size:
1567 1283 826 580 129
ApaI G_GGCC'C
Cuts at: 291 322 291
Size: 31 4354
ApaLI G'TGCA_C
Cuts at: 3155 3155
Size: 4385
ApoI r'AATT_y
Cuts at: 1389 3738 3994 1389
Size: 2349 256 1780
Fragments arranged by size:
2349 1780 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4385
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
AvaI C'yCGr_G
Cuts at: 1131 1405 1131
Size: 274 4111
BamHI G'GATC_C
Cuts at: 1410 1410
Size: 4385
BanI G'GyrC_C
Cuts at: 203 1108 1310 1375 1401 1815 2063 2098 203
Size: 905 202 65 26 414 248 35 2490
Fragments arranged by size:
2490 905 414 248 202 65 35 26
BanII G_rGCy'C
Cuts at: 291 322 1066 1399 2429 291
Size: 31 744 333 1030 2247
Fragments arranged by size:
2247 1030 744 333 31
BbeI G_GCGC'C
Cuts at: 1379 1819 2067 1379
Size: 440 248 3697
Fragments arranged by size:
3697 440 248
BbsI GAAGACnn'nnnn_
Cuts at: 607 715 607
Size: 108 4277
BciVI GTATCCnnnnn_n'
Cuts at: 2283 3044 4385 2283
Size: 761 1341 2283
Fragments arranged by size:
2283 1341 761
BclI T'GATC_A
Cuts at: 1905 1905
Size: 4385
BglI GCCn_nnn'nGGC
Cuts at: 700 1340 700
Size: 640 3745
BglII A'GATC_T
Cuts at: 1900 3937 1900
Size: 2037 2348
BlpI GC'TnA_GC
Cuts at: 371 379 1252 1497 371
Size: 8 873 245 3259
Fragments arranged by size:
3259 873 245 8
Bme1580I G_kGCm'C
Cuts at: 206 291 322 1218 2010 2103 3159 206
Size: 85 31 896 792 93 1056 1432
Fragments arranged by size:
1432 1056 896 792 93 85 31
BmrI ACTGGGnnnn_n'
Cuts at: 294 318 1119 1597 1678 2013 4157 294
Size: 24 801 478 81 335 2144 522
Fragments arranged by size:
2144 801 522 478 335 81 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 149 358 415 98
Size: 51 209 57 4068
Fragments arranged by size:
4068 209 57 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1561 2440 2932 3230 1561
Size: 879 492 298 2716
Fragments arranged by size:
2716 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 787 4089 787
Size: 3302 1083
BsaAI yAC'GTr
Cuts at: 1362 1738 1362
Size: 376 4009
BsaBI GATnn'nnATC
Cuts at: 6 1899 3936 6
Size: 1893 2037 455
Fragments arranged by size:
2037 1893 455
BsaHI Gr'CG_yC
Cuts at: 1376 1816 2064 2766 4262 1376
Size: 440 248 702 1496 1499
Fragments arranged by size:
1499 1496 702 440 248
BsaWI w'CCGG_w
Cuts at: 1574 2095 2773 3047 3194 3605 1574
Size: 521 678 274 147 411 2354
Fragments arranged by size:
2354 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1021 1174 1285 1306 1021
Size: 153 111 21 4100
Fragments arranged by size:
4100 153 111 21
BseYI C'CCAG_C
Cuts at: 269 474 1810 2568 3145 3641 269
Size: 205 1336 758 577 496 1013
Fragments arranged by size:
1336 1013 758 577 496 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 373
Size: 4385
BsiEI CG_ry'CG
Cuts at: 104 503 1927 1973 3181 104
Size: 399 1424 46 1208 1308
Fragments arranged by size:
1424 1308 1208 399 46
BsiHKAI G_wGCw'C
Cuts at: 1399 2177 2367 3159 1399
Size: 778 190 792 2625
Fragments arranged by size:
2625 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 651 651
Size: 4385
Bsp1286I G_dGCh'C
Cuts at: 206 291 322 1066 1218 1399 2010 2103 2177
Size: 85 31 744 152 181 611 93 74
Cuts at: 2177 2367 2429 3159 206
Size: 190 62 730 1432
Fragments arranged by size:
1432 744 730 611 190 181 152 93
85 74 62 31
BspEI T'CCGG_A
Cuts at: 3605 3605
Size: 4385
BspHI T'CATG_A
Cuts at: 3561 3561
Size: 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 805 1435 1649 1951 2332 805
Size: 630 214 302 381 2858
Fragments arranged by size:
2858 630 381 302 214
BsrBI CCG'CTC
Cuts at: 50 375 432 2677 2731 4215 4380 50
Size: 325 57 2245 54 1484 165 55
Fragments arranged by size:
2245 1484 325 165 57 55 54
BsrDI GCAATG_nn'
Cuts at: 2297 2297
Size: 4385
BsrFI r'CCGG_y
Cuts at: 206 464 2383 2564 2773 206
Size: 258 1919 181 209 1818
Fragments arranged by size:
1919 1818 258 209 181
BssHII G'CGCG_C
Cuts at: 243 383 438 243
Size: 140 55 4190
Fragments arranged by size:
4190 140 55
BssSI C'ACGA_G
Cuts at: 2656 3014 2656
Size: 358 4027
BstAPI GCAn_nnn'nTGC
Cuts at: 1762 1762
Size: 4385
BstBI TT'CG_AA
Cuts at: 2746 2746
Size: 4385
BstEII G'GTnAC_C
Cuts at: 1074 1074
Size: 4385
BstXI CCAn_nnnn'nTGG
Cuts at: 1362 1362
Size: 4385
BstYI r'GATC_y
Cuts at: 1 945 1410 1877 1900 2235 2481 3493 3579
Size: 944 465 467 23 335 246 1012 86
Cuts at: 3579 3591 3937 1
Size: 12 346 449
Fragments arranged by size:
1012 944 467 465 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 141 141
Size: 4385
BtgI C'CryG_G
Cuts at: 107 308 969 107
Size: 201 661 3523
Fragments arranged by size:
3523 661 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 733 2335 2516 2679 733
Size: 1602 181 163 2439
Fragments arranged by size:
2439 1602 181 163
BtsI GCAGTG_nn'
Cuts at: 885 1628 885
Size: 743 3642
DraI TTT'AAA
Cuts at: 1384 1384
Size: 4385
DrdI GACnn_nn'nnGTC
Cuts at: 643 2091 2949 643
Size: 1448 858 2079
Fragments arranged by size:
2079 1448 858
EaeI y'GGCC_r
Cuts at: 500 983 994 1056 1233 1332 1970 2144 2535
Size: 483 11 62 177 99 638 174 391
Cuts at: 2535 2562 3907 500
Size: 27 1345 978
Fragments arranged by size:
1345 978 638 483 391 177 174 99
62 27 11
EagI C'GGCC_G
Cuts at: 500 1970 500
Size: 1470 2915
EarI CTCTTCn'nnn_
Cuts at: 113 535 935 1169 2408 2618 113
Size: 422 400 234 1239 210 1880
Fragments arranged by size:
1880 1239 422 400 234 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2593 2903 3049 4195 2593
Size: 310 146 1146 2783
Fragments arranged by size:
2783 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1397 1397
Size: 4385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 149 358 415 559 568 1300 2210 2642
Size: 51 209 57 144 9 732 910 432
Cuts at: 2642 3389 3657 98
Size: 747 268 826
Fragments arranged by size:
910 826 747 732 432 268 209 144
57 51 9
EcoO109I rG'GnC_Cy
Cuts at: 219 318 319 1136 1524 219
Size: 99 1 817 388 3080
Fragments arranged by size:
3080 817 388 99 1
EcoRI G'AATT_C
Cuts at: 1389 1389
Size: 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2404 2404
Size: 4385
FspI TGC'GCA
Cuts at: 664 2166 664
Size: 1502 2883
HaeII r_GCGC'y
Cuts at: 644 816 1379 1819 2067 3089 4068 644
Size: 172 563 440 248 1022 979 961
Fragments arranged by size:
1022 979 961 563 440 248 172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 702 734 997 1029 1150 1182 2227 2257 2259
Size: 32 263 32 121 32 1045 30 2
Cuts at: 2259 2289 702
Size: 30 2798
Fragments arranged by size:
2798 1045 263 121 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1424 1424
Size: 4385
KasI G'GCGC_C
Cuts at: 1375 1815 2063 1375
Size: 440 248 3697
Fragments arranged by size:
3697 440 248
KpnI G_GTAC'C
Cuts at: 1405 1405
Size: 4385
MluI A'CGCG_T
Cuts at: 3818 3818
Size: 4385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3056 3240 3056
Size: 184 4201
MscI TGG'CCA
Cuts at: 985 996 1235 2146 985
Size: 11 239 911 3224
Fragments arranged by size:
3224 911 239 11
MslI CAynn'nnrTG
Cuts at: 985 2374 2501 985
Size: 1389 127 2869
Fragments arranged by size:
2869 1389 127
MspA1I CmG'CkG
Cuts at: 189 230 450 660 819 1496 1810 2170 3183
Size: 41 220 210 159 677 314 360 1013
Cuts at: 3183 3428 189
Size: 245 1146
Fragments arranged by size:
1146 1013 677 360 314 245 220 210
159 41
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 4385
NarI GG'CG_CC
Cuts at: 1376 1816 2064 1376
Size: 440 248 3697
Fragments arranged by size:
3697 440 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4385
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 4385
NspI r_CATG'y
Cuts at: 355 1438 1938 2845 355
Size: 1083 500 907 1895
Fragments arranged by size:
1895 1083 907 500
PciI A'CATG_T
Cuts at: 2841 2841
Size: 4385
PflMI CCAn_nnn'nTGG
Cuts at: 276 864 992 276
Size: 588 128 3669
Fragments arranged by size:
3669 588 128
PfoI T'CCnGG_A
Cuts at: 236 236
Size: 4385
PmeI GTTT'AAAC
Cuts at: 1384 1384
Size: 4385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3524 3556 3524
Size: 32 4353
PpuMI rG'GwC_Cy
Cuts at: 219 1136 219
Size: 917 3468
PspOMI G'GGCC_C
Cuts at: 287 318 287
Size: 31 4354
PstI C_TGCA'G
Cuts at: 674 1432 2117 674
Size: 758 685 2942
Fragments arranged by size:
2942 758 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4385
PvuII CAG'CTG
Cuts at: 230 660 1810 2170 230
Size: 430 1150 360 2445
Fragments arranged by size:
2445 1150 430 360
RsrII CG'GwC_CG
Cuts at: 2580 2580
Size: 4385
SacI G_AGCT'C
Cuts at: 1399 1399
Size: 4385
SalI G'TCGA_C
Cuts at: 1422 1422
Size: 4385
SanDI GG'GwC_CC
Cuts at: 1136 1136
Size: 4385
SapI GCTCTTCn'nnn_
Cuts at: 2408 2618 2408
Size: 210 4175
SbfI CC_TGCA'GG
Cuts at: 1432 1432
Size: 4385
ScaI AGT'ACT
Cuts at: 3902 3902
Size: 4385
SexAI A'CCwGG_T
Cuts at: 221 221
Size: 4385
SfcI C'TryA_G
Cuts at: 33 171 670 1428 2113 3106 3297 33
Size: 138 499 758 685 993 191 1121
Fragments arranged by size:
1121 993 758 685 499 191 138
SfiI GGCCn_nnn'nGGCC
Cuts at: 1340 1340
Size: 4385
SfoI GGC'GCC
Cuts at: 1377 1817 2065 1377
Size: 440 248 3697
Fragments arranged by size:
3697 440 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4385
SmaI CCC'GGG
Cuts at: 1133 1407 1133
Size: 274 4111
SmlI C'TyrA_G
Cuts at: 1540 2455 2947 3209 1540
Size: 915 492 262 2716
Fragments arranged by size:
2716 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4385
SphI G_CATG'C
Cuts at: 355 1438 1938 355
Size: 1083 500 2802
Fragments arranged by size:
2802 1083 500
StuI AGG'CCT
Cuts at: 139 346 787 139
Size: 207 441 3737
Fragments arranged by size:
3737 441 207
StyI C'CwwG_G
Cuts at: 107 695 1016 1314 1519 1873 107
Size: 588 321 298 205 354 2619
Fragments arranged by size:
2619 588 354 321 298 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 982 1968 2101 2560 2769 982
Size: 986 133 459 209 2598
Fragments arranged by size:
2598 986 459 209 133
TatI w'GTAC_w
Cuts at: 3900 3900
Size: 4385
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1147 1179 3902 3934 1147
Size: 32 2723 32 1598
Fragments arranged by size:
2723 1598 32 32
Tth111I GACn'n_nGTC
Cuts at: 223 2182 223
Size: 1959 2426
XbaI T'CTAG_A
Cuts at: 1416 1416
Size: 4385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1327 1880 1327
Size: 553 3832
XmaI C'CCGG_G
Cuts at: 1131 1405 1131
Size: 274 4111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BsiWI BsmI BsrGI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI