Restriction map for
pF1KB6666
(Circular) (Six-base) MAPSORT of: pF1KB6666.seq Check: 6882 from: 1 to: 4472
pF1KB6666 4472 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 274 293 725 1510 274
Size: 19 432 785 3236
Fragments arranged by size:
3236 785 432 19
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 4312 4312
Size: 4472
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 2297 2729 3476 3744 1013
Size: 1284 432 747 268 1741
Fragments arranged by size:
1741 1284 747 432 268
AflIII A'CryG_T
Cuts at: 175 1824 2928 3905 175
Size: 1649 1104 977 742
Fragments arranged by size:
1649 1104 977 742
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 1127 1777 3344 1127
Size: 650 1567 2255
Fragments arranged by size:
2255 1567 650
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 132 859 1476 3825 4081 132
Size: 727 617 2349 256 523
Fragments arranged by size:
2349 727 617 523 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 1492 1492
Size: 4472
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 1488 1902 2150 2185 1488
Size: 414 248 35 3775
Fragments arranged by size:
3775 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3442
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 323 944 323
Size: 621 3851
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3131 4472 2370
Size: 761 1341 2370
Fragments arranged by size:
2370 1341 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BfrBI ATG'CAT
Cuts at: 919 919
Size: 4472
BglII A'GATC_T
Cuts at: 1987 4024 1987
Size: 2037 2435
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 2097 2190 3246 2097
Size: 93 1056 3323
Fragments arranged by size:
3323 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 641 1684 1765 2100 4244 641
Size: 1043 81 335 2144 869
Fragments arranged by size:
2144 1043 869 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1064 1064
Size: 4472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 1648 2527 3019 3317 483
Size: 1165 879 492 298 1638
Fragments arranged by size:
1638 1165 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4176 4176
Size: 4472
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 1903 2151 2853 4349 1903
Size: 248 702 1496 2026
Fragments arranged by size:
2026 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 572 867 1897 2655 3232 3728 572
Size: 295 1030 758 577 496 1316
Fragments arranged by size:
1316 1030 758 577 496 295
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1421 1486 2264 2454 3246 1421
Size: 65 778 190 792 2647
Fragments arranged by size:
2647 792 778 190 65
Bsp1286I G_dGCh'C
Cuts at: 1421 1486 2097 2190 2264 2454 2516 3246 1421
Size: 65 611 93 74 190 62 730 2647
Fragments arranged by size:
2647 730 611 190 93 74 65 62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1522 1736 2038 2419 1522
Size: 214 302 381 3575
Fragments arranged by size:
3575 381 302 214
BsrBI CCG'CTC
Cuts at: 50 677 2764 2818 4302 4467 50
Size: 627 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 627 165 55 54
BsrDI GCAATG_nn'
Cuts at: 257 650 2384 257
Size: 393 1734 2345
Fragments arranged by size:
2345 1734 393
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 190 970 1849 190
Size: 780 879 2813
Fragments arranged by size:
2813 879 780
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstXI CCAn_nnnn'nTGG
Cuts at: 1219 1219
Size: 4472
BstYI r'GATC_y
Cuts at: 1 300 1177 1497 1964 1987 2322 2568 3580
Size: 299 877 320 467 23 335 246 1012
Cuts at: 3580 3666 3678 4024 1
Size: 86 12 346 449
Fragments arranged by size:
1012 877 467 449 346 335 320 299
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1454 1454
Size: 4472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1088 2422 2603 2766 1088
Size: 1334 181 163 2794
Fragments arranged by size:
2794 1334 181 163
BtsI GCAGTG_nn'
Cuts at: 792 1715 792
Size: 923 3549
ClaI AT'CG_AT
Cuts at: 481 737 481
Size: 256 4216
DraI TTT'AAA
Cuts at: 1471 1471
Size: 4472
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 1209 2057 2231 2622 2649 3994 1209
Size: 848 174 391 27 1345 1687
Fragments arranged by size:
1687 1345 848 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 174 766 2495 2705 174
Size: 592 1729 210 1941
Fragments arranged by size:
1941 1729 592 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1013 1064 2297 2729 3476 3744 1013
Size: 51 1233 432 747 268 1741
Fragments arranged by size:
1741 1233 747 432 268 51
EcoO109I rG'GnC_Cy
Cuts at: 988 1611 988
Size: 623 3849
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
EcoRV GAT'ATC
Cuts at: 627 627
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4472
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 256 288 292 324 388 420 2314 2344 2346
Size: 32 4 32 64 32 1894 30 2
Cuts at: 2346 2376 256
Size: 30 2352
Fragments arranged by size:
2352 1894 64 32 32 32 30 30
4 2
HincII GTy'rAC
Cuts at: 552 1511 552
Size: 959 3513
HpaI GTT'AAC
Cuts at: 552 552
Size: 4472
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MfeI C'AATT_G
Cuts at: 1364 1364
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 268 402 3143 3327 268
Size: 134 2741 184 1413
Fragments arranged by size:
2741 1413 184 134
MscI TGG'CCA
Cuts at: 1211 2233 1211
Size: 1022 3450
MslI CAynn'nnrTG
Cuts at: 1217 2461 2588 1217
Size: 1244 127 3101
Fragments arranged by size:
3101 1244 127
MspA1I CmG'CkG
Cuts at: 363 1583 1897 2257 3270 3515 363
Size: 1220 314 360 1013 245 1320
Fragments arranged by size:
1320 1220 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NdeI CA'TA_TG
Cuts at: 186 915 1017 186
Size: 729 102 3641
Fragments arranged by size:
3641 729 102
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NsiI A_TGCA'T
Cuts at: 921 921
Size: 4472
NspI r_CATG'y
Cuts at: 179 1116 1525 2025 2932 179
Size: 937 409 500 907 1719
Fragments arranged by size:
1719 937 907 500 409
PciI A'CATG_T
Cuts at: 175 2928 175
Size: 2753 1719
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4472
PfoI T'CCnGG_A
Cuts at: 442 442
Size: 4472
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PpuMI rG'GwC_Cy
Cuts at: 988 988
Size: 4472
PshAI GACnn'nnGTC
Cuts at: 837 837
Size: 4472
PsiI TTA'TAA
Cuts at: 389 389
Size: 4472
PstI C_TGCA'G
Cuts at: 966 1519 2204 966
Size: 553 685 3234
Fragments arranged by size:
3234 685 553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 363 1897 2257 363
Size: 1534 360 2578
Fragments arranged by size:
2578 1534 360
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 1509 1509
Size: 4472
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 1519 1519
Size: 4472
ScaI AGT'ACT
Cuts at: 960 3989 960
Size: 3029 1443
SfcI C'TryA_G
Cuts at: 33 304 962 1515 2200 3193 3384 33
Size: 271 658 553 685 993 191 1121
Fragments arranged by size:
1121 993 685 658 553 271 191
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 498 1627 2542 3034 3296 498
Size: 1129 915 492 262 1674
Fragments arranged by size:
1674 1129 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1116 1525 2025 1116
Size: 409 500 3563
Fragments arranged by size:
3563 500 409
StyI C'CwwG_G
Cuts at: 1233 1606 1960 1233
Size: 373 354 3745
Fragments arranged by size:
3745 373 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2055 2188 2647 2856 2055
Size: 133 459 209 3671
Fragments arranged by size:
3671 459 209 133
TatI w'GTAC_w
Cuts at: 958 3987 958
Size: 3029 1443
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 375 407 746 778 3989 4021 375
Size: 32 339 32 3211 32 826
Fragments arranged by size:
3211 826 339 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2269 2269
Size: 4472
XbaI T'CTAG_A
Cuts at: 1503 1503
Size: 4472
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 628 981 1967 628
Size: 353 986 3133
Fragments arranged by size:
3133 986 353
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I BtgI CspCI DraIII EcoNI
FseI FspAI HindIII NcoI NheI NotI NruI PacI
PasI PmlI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI