Restriction map for
pF1KB6667
(Circular) (Six-base) MAPSORT of: pF1KB6667.seq Check: 2387 from: 1 to: 4388
pF1KB6667 4388 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 983 2213 2645 3392 3660 458
Size: 525 1230 432 747 268 1186
Fragments arranged by size:
1230 1186 747 525 432 268
AfeI AGC'GCT
Cuts at: 683 683
Size: 4388
AflII C'TTAA_G
Cuts at: 594 594
Size: 4388
AflIII A'CryG_T
Cuts at: 1740 2844 3821 1740
Size: 1104 977 2307
Fragments arranged by size:
2307 1104 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 514 514
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 619 1693 3260 619
Size: 1074 1567 1747
Fragments arranged by size:
1747 1567 1074
ApaLI G'TGCA_C
Cuts at: 722 3158 722
Size: 2436 1952
ApoI r'AATT_y
Cuts at: 303 550 799 910 974 1297 1392 3741 3997
Size: 247 249 111 64 323 95 2349 256
Cuts at: 3997 303
Size: 694
Fragments arranged by size:
2349 694 323 256 249 247 111 95
64
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 1179 1286 1408 1179
Size: 107 122 4159
Fragments arranged by size:
4159 122 107
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1044 1077 1044
Size: 33 4355
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 1404 1818 2066 2101 1404
Size: 414 248 35 3691
Fragments arranged by size:
3691 414 248 35
BanII G_rGCy'C
Cuts at: 1402 2432 1402
Size: 1030 3358
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BbsI GAAGACnn'nnnn_
Cuts at: 353 447 532 779 353
Size: 94 85 247 3962
Fragments arranged by size:
3962 247 94 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1267 1301 1267
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3047 4388 2286
Size: 761 1341 2286
Fragments arranged by size:
2286 1341 761
BclI T'GATC_A
Cuts at: 381 1908 381
Size: 1527 2861
BfrBI ATG'CAT
Cuts at: 167 566 1114 167
Size: 399 548 3441
Fragments arranged by size:
3441 548 399
BglII A'GATC_T
Cuts at: 1903 3940 1903
Size: 2037 2351
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 726 2013 2106 3162 726
Size: 1287 93 1056 1952
Fragments arranged by size:
1952 1287 1056 93
BmgBI CAC'GTC
Cuts at: 887 887
Size: 4388
BmrI ACTGGGnnnn_n'
Cuts at: 1600 1681 2016 4160 1600
Size: 81 335 2144 1828
Fragments arranged by size:
2144 1828 335 81
BmtI G_CTAG'C
Cuts at: 574 574
Size: 4388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2755 2755
Size: 4388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1121 839
Size: 282 4106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 1564 2443 2935 3233 296
Size: 1268 879 492 298 1451
Fragments arranged by size:
1451 1268 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 855 1097 4092 855
Size: 242 2995 1151
Fragments arranged by size:
2995 1151 242
BsaAI yAC'GTr
Cuts at: 232 1741 232
Size: 1509 2879
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1367 1397 1367
Size: 30 4358
BseYI C'CCAG_C
Cuts at: 1813 2571 3148 3644 1813
Size: 758 577 496 2557
Fragments arranged by size:
2557 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 780
Size: 4388
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 640 726 1402 2180 2370 3162 640
Size: 86 676 778 190 792 1866
Fragments arranged by size:
1866 792 778 676 190 86
Bsp1286I G_dGCh'C
Cuts at: 640 726 1402 2013 2106 2180 2370 2432 3162
Size: 86 676 611 93 74 190 62 730
Cuts at: 3162 640
Size: 1866
Fragments arranged by size:
1866 730 676 611 190 93 86 74
62
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2300 2300
Size: 4388
BsrFI r'CCGG_y
Cuts at: 2386 2567 2776 2386
Size: 181 209 3998
Fragments arranged by size:
3998 209 181
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstYI r'GATC_y
Cuts at: 1 537 1413 1880 1903 2238 2484 3496 3582
Size: 536 876 467 23 335 246 1012 86
Cuts at: 3582 3594 3940 1
Size: 12 346 449
Fragments arranged by size:
1012 876 536 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 695 695
Size: 4388
BtgI C'CryG_G
Cuts at: 107 107
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 617 902 1631 617
Size: 285 729 3374
Fragments arranged by size:
3374 729 285
DraI TTT'AAA
Cuts at: 1387 1387
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 1973 2147 2538 2565 3910 1973
Size: 174 391 27 1345 2451
Fragments arranged by size:
2451 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2596 2906 3052 4198 2596
Size: 310 146 1146 2786
Fragments arranged by size:
2786 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1400 1400
Size: 4388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 458 839 983 1121 2213 2645 3392 3660 458
Size: 381 144 138 1092 432 747 268 1186
Fragments arranged by size:
1186 1092 747 432 381 268 144 138
EcoO109I rG'GnC_Cy
Cuts at: 1527 1527
Size: 4388
EcoRI G'AATT_C
Cuts at: 303 1297 1392 303
Size: 994 95 3299
Fragments arranged by size:
3299 994 95
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 685 1822 2070 3092 4071 685
Size: 1137 248 1022 979 1002
Fragments arranged by size:
1137 1022 1002 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 366 398 817 849 854 886 1228 1260 2230
Size: 32 419 32 5 32 342 32 970
Cuts at: 2230 2260 2262 2292 366
Size: 30 2 30 2462
Fragments arranged by size:
2462 970 419 342 32 32 32 32
30 30 5 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3059 3243 3059
Size: 184 4204
MscI TGG'CCA
Cuts at: 2149 2149
Size: 4388
MslI CAynn'nnrTG
Cuts at: 1370 2377 2504 1370
Size: 1007 127 3254
Fragments arranged by size:
3254 1007 127
MspA1I CmG'CkG
Cuts at: 249 1499 1813 2173 3186 3431 249
Size: 1250 314 360 1013 245 1206
Fragments arranged by size:
1250 1206 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NheI G'CTAG_C
Cuts at: 570 570
Size: 4388
NsiI A_TGCA'T
Cuts at: 169 568 1116 169
Size: 399 548 3441
Fragments arranged by size:
3441 548 399
NspI r_CATG'y
Cuts at: 167 570 1441 1941 2848 167
Size: 403 871 500 907 1707
Fragments arranged by size:
1707 907 871 500 403
PasI CC'CwG_GG
Cuts at: 937 937
Size: 4388
PciI A'CATG_T
Cuts at: 2844 2844
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PspXI vC'TCGA_Gb
Cuts at: 1286 1286
Size: 4388
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1265 1297 1265
Size: 32 4356
PstI C_TGCA'G
Cuts at: 186 1435 2120 186
Size: 1249 685 2454
Fragments arranged by size:
2454 1249 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 249 1813 2173 249
Size: 1564 360 2464
Fragments arranged by size:
2464 1564 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1402 1402
Size: 4388
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 182 1431 2116 3109 3300 33
Size: 149 1249 685 993 191 1121
Fragments arranged by size:
1249 1121 993 685 191 149
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1181 1410 1181
Size: 229 4159
SmlI C'TyrA_G
Cuts at: 311 594 1286 1543 2458 2950 3212 311
Size: 283 692 257 915 492 262 1487
Fragments arranged by size:
1487 915 692 492 283 262 257
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 167 570 1441 1941 167
Size: 403 871 500 2614
Fragments arranged by size:
2614 871 500 403
StyI C'CwwG_G
Cuts at: 107 1522 1876 107
Size: 1415 354 2619
Fragments arranged by size:
2619 1415 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1971 2104 2563 2772 1971
Size: 133 459 209 3587
Fragments arranged by size:
3587 459 209 133
TatI w'GTAC_w
Cuts at: 3903 3903
Size: 4388
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 3905
Size: 32 4356
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XhoI C'TCGA_G
Cuts at: 1286 1286
Size: 4388
XmaI C'CCGG_G
Cuts at: 1179 1408 1179
Size: 229 4159
XmnI GAAnn'nnTTC
Cuts at: 529 551 529
Size: 22 4366
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PasI PciI PmeI PpiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AhdI AleI AloI ApaI AscI AvrII
BbvCI BglI Bpu10I BseRI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NotI
NruI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI ZraI