Restriction map for
pF1KB6668
(Circular) (Six-base) MAPSORT of: pF1KB6668.seq Check: 2104 from: 1 to: 4472
pF1KB6668 4472 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 422 1441 1510 422
Size: 1019 69 3384
Fragments arranged by size:
3384 1019 69
Acc65I G'GTAC_C
Cuts at: 1488 1488
Size: 4472
AclI AA'CG_TT
Cuts at: 285 4312 285
Size: 4027 445
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1099 1199 2297 2729 3476 3744 310
Size: 789 100 1098 432 747 268 1038
Fragments arranged by size:
1098 1038 789 747 432 268 100
AflIII A'CryG_T
Cuts at: 162 1824 2928 3905 162
Size: 1662 1104 977 729
Fragments arranged by size:
1662 1104 977 729
AgeI A'CCGG_T
Cuts at: 2860 2860
Size: 4472
AhdI GACnn_n'nnGTC
Cuts at: 841 841
Size: 4472
AlwNI CAG_nnn'CTG
Cuts at: 208 807 1380 1777 3344 208
Size: 599 573 397 1567 1336
Fragments arranged by size:
1567 1336 599 573 397
ApaLI G'TGCA_C
Cuts at: 3242 3242
Size: 4472
ApoI r'AATT_y
Cuts at: 1476 3825 4081 1476
Size: 2349 256 1867
Fragments arranged by size:
2349 1867 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4472
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
AvaI C'yCGr_G
Cuts at: 1492 1492
Size: 4472
BamHI G'GATC_C
Cuts at: 1497 1497
Size: 4472
BanI G'GyrC_C
Cuts at: 585 1488 1902 2150 2185 585
Size: 903 414 248 35 2872
Fragments arranged by size:
2872 903 414 248 35
BanII G_rGCy'C
Cuts at: 1486 2516 1486
Size: 1030 3442
BbeI G_GCGC'C
Cuts at: 1906 2154 1906
Size: 248 4224
BbsI GAAGACnn'nnnn_
Cuts at: 309 829 1398 309
Size: 520 569 3383
Fragments arranged by size:
3383 569 520
BbvCI CC'TCA_GC
Cuts at: 251 1397 251
Size: 1146 3326
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 112 146 112
Size: 34 4438
BciVI GTATCCnnnnn_n'
Cuts at: 2370 3131 4472 2370
Size: 761 1341 2370
Fragments arranged by size:
2370 1341 761
BclI T'GATC_A
Cuts at: 1992 1992
Size: 4472
BfrBI ATG'CAT
Cuts at: 385 385
Size: 4472
BglI GCCn_nnn'nGGC
Cuts at: 996 996
Size: 4472
BglII A'GATC_T
Cuts at: 593 912 1424 1987 4024 593
Size: 319 512 563 2037 1041
Fragments arranged by size:
2037 1041 563 512 319
BlpI GC'TnA_GC
Cuts at: 1584 1584
Size: 4472
Bme1580I G_kGCm'C
Cuts at: 588 1366 2097 2190 3246 588
Size: 778 731 93 1056 1814
Fragments arranged by size:
1814 1056 778 731 93
BmrI ACTGGGnnnn_n'
Cuts at: 1684 1765 2100 4244 1684
Size: 81 335 2144 1912
Fragments arranged by size:
2144 1912 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 4472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1156
Size: 4472
Bpu10I CC'TnA_GC
Cuts at: 251 1397 251
Size: 1146 3326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1080 1648 2527 3019 3317 1080
Size: 568 879 492 298 2235
Fragments arranged by size:
2235 879 568 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1149 4176 1149
Size: 3027 1445
BsaAI yAC'GTr
Cuts at: 1825 1825
Size: 4472
BsaBI GATnn'nnATC
Cuts at: 6 1986 4023 6
Size: 1980 2037 455
Fragments arranged by size:
2037 1980 455
BsaHI Gr'CG_yC
Cuts at: 842 1336 1903 2151 2853 4349 842
Size: 494 567 248 702 1496 965
Fragments arranged by size:
1496 965 702 567 494 248
BsaWI w'CCGG_w
Cuts at: 1661 2182 2860 3134 3281 3692 1661
Size: 521 678 274 147 411 2441
Fragments arranged by size:
2441 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 475 1327 475
Size: 852 3620
BseYI C'CCAG_C
Cuts at: 171 1897 2655 3232 3728 171
Size: 1726 758 577 496 915
Fragments arranged by size:
1726 915 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1437 1437
Size: 4472
BsiEI CG_ry'CG
Cuts at: 104 2014 2060 3268 104
Size: 1910 46 1208 1308
Fragments arranged by size:
1910 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1486 2264 2454 3246 1486
Size: 778 190 792 2712
Fragments arranged by size:
2712 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 588 1366 1486 2097 2190 2264 2454 2516 3246
Size: 778 120 611 93 74 190 62 730
Cuts at: 3246 588
Size: 1814
Fragments arranged by size:
1814 778 730 611 190 120 93 74
62
BspEI T'CCGG_A
Cuts at: 3692 3692
Size: 4472
BspHI T'CATG_A
Cuts at: 3648 3648
Size: 4472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 128 1522 1736 2038 2419 128
Size: 1394 214 302 381 2181
Fragments arranged by size:
2181 1394 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2764 2818 4302 4467 50
Size: 2714 54 1484 165 55
Fragments arranged by size:
2714 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2384 2384
Size: 4472
BsrFI r'CCGG_y
Cuts at: 2470 2651 2860 2470
Size: 181 209 4082
Fragments arranged by size:
4082 209 181
BssSI C'ACGA_G
Cuts at: 2743 3101 2743
Size: 358 4114
BstAPI GCAn_nnn'nTGC
Cuts at: 1849 1849
Size: 4472
BstBI TT'CG_AA
Cuts at: 2833 2833
Size: 4472
BstEII G'GTnAC_C
Cuts at: 561 561
Size: 4472
BstXI CCAn_nnnn'nTGG
Cuts at: 639 639
Size: 4472
BstYI r'GATC_y
Cuts at: 1 593 912 1208 1424 1497 1964 1987 2322
Size: 592 319 296 216 73 467 23 335
Cuts at: 2322 2568 3580 3666 3678 4024 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 592 467 449 346 335 319 296
246 216 86 73 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4472
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2422 2603 2766 2422
Size: 181 163 4128
Fragments arranged by size:
4128 181 163
BtsI GCAGTG_nn'
Cuts at: 507 1715 507
Size: 1208 3264
DraI TTT'AAA
Cuts at: 275 1471 275
Size: 1196 3276
DrdI GACnn_nn'nnGTC
Cuts at: 2178 3036 2178
Size: 858 3614
EaeI y'GGCC_r
Cuts at: 399 851 2057 2231 2622 2649 3994 399
Size: 452 1206 174 391 27 1345 877
Fragments arranged by size:
1345 1206 877 452 391 174 27
EagI C'GGCC_G
Cuts at: 2057 2057
Size: 4472
EarI CTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2680 2990 3136 4282 2680
Size: 310 146 1146 2870
Fragments arranged by size:
2870 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1484 1484
Size: 4472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 310 1099 1156 1199 2297 2729 3476 3744 310
Size: 789 57 43 1098 432 747 268 1038
Fragments arranged by size:
1098 1038 789 747 432 268 57 43
EcoNI CCTnn'n_nnAGG
Cuts at: 302 460 763 1171 302
Size: 158 303 408 3603
Fragments arranged by size:
3603 408 303 158
EcoO109I rG'GnC_Cy
Cuts at: 545 714 1317 1611 545
Size: 169 603 294 3406
Fragments arranged by size:
3406 603 294 169
EcoRI G'AATT_C
Cuts at: 1476 1476
Size: 4472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4472
FspI TGC'GCA
Cuts at: 2253 2253
Size: 4472
HaeII r_GCGC'y
Cuts at: 1906 2154 3176 4155 1906
Size: 248 1022 979 2223
Fragments arranged by size:
2223 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 181 213 827 859 2314 2344 2346 2376 181
Size: 32 614 32 1455 30 2 30 2277
Fragments arranged by size:
2277 1455 614 32 32 30 30 2
HincII GTy'rAC
Cuts at: 423 651 1511 423
Size: 228 860 3384
Fragments arranged by size:
3384 860 228
KasI G'GCGC_C
Cuts at: 1902 2150 1902
Size: 248 4224
KpnI G_GTAC'C
Cuts at: 1492 1492
Size: 4472
MluI A'CGCG_T
Cuts at: 3905 3905
Size: 4472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3143 3327 3143
Size: 184 4288
MscI TGG'CCA
Cuts at: 401 853 2233 401
Size: 452 1380 2640
Fragments arranged by size:
2640 1380 452
MslI CAynn'nnrTG
Cuts at: 941 2461 2588 941
Size: 1520 127 2825
Fragments arranged by size:
2825 1520 127
MspA1I CmG'CkG
Cuts at: 1326 1583 1897 2257 3270 3515 1326
Size: 257 314 360 1013 245 2283
Fragments arranged by size:
2283 1013 360 314 257 245
NaeI GCC'GGC
Cuts at: 2653 2653
Size: 4472
NarI GG'CG_CC
Cuts at: 1903 2151 1903
Size: 248 4224
NcoI C'CATG_G
Cuts at: 107 107
Size: 4472
NgoMIV G'CCGG_C
Cuts at: 2651 2651
Size: 4472
NsiI A_TGCA'T
Cuts at: 387 387
Size: 4472
NspI r_CATG'y
Cuts at: 166 1525 2025 2932 166
Size: 1359 500 907 1706
Fragments arranged by size:
1706 1359 907 500
PciI A'CATG_T
Cuts at: 162 2928 162
Size: 2766 1706
PfoI T'CCnGG_A
Cuts at: 638 1052 1172 638
Size: 414 120 3938
Fragments arranged by size:
3938 414 120
PmeI GTTT'AAAC
Cuts at: 1471 1471
Size: 4472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3611 3643 3611
Size: 32 4440
PpuMI rG'GwC_Cy
Cuts at: 545 1317 545
Size: 772 3700
PstI C_TGCA'G
Cuts at: 125 146 581 615 893 1519 2204 125
Size: 21 435 34 278 626 685 2393
Fragments arranged by size:
2393 685 626 435 278 34 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4472
PvuII CAG'CTG
Cuts at: 1897 2257 1897
Size: 360 4112
RsrII CG'GwC_CG
Cuts at: 2667 2667
Size: 4472
SacI G_AGCT'C
Cuts at: 1486 1486
Size: 4472
SalI G'TCGA_C
Cuts at: 421 1509 421
Size: 1088 3384
SapI GCTCTTCn'nnn_
Cuts at: 2495 2705 2495
Size: 210 4262
SbfI CC_TGCA'GG
Cuts at: 125 581 1519 125
Size: 456 938 3078
Fragments arranged by size:
3078 938 456
ScaI AGT'ACT
Cuts at: 3989 3989
Size: 4472
SfcI C'TryA_G
Cuts at: 33 121 142 577 611 831 889 1515 2200
Size: 88 21 435 34 220 58 626 685
Cuts at: 2200 3193 3384 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 685 626 435 220 191 88
58 34 21
SfoI GGC'GCC
Cuts at: 1904 2152 1904
Size: 248 4224
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4472
SmaI CCC'GGG
Cuts at: 1494 1494
Size: 4472
SmlI C'TyrA_G
Cuts at: 1095 1627 2542 3034 3296 1095
Size: 532 915 492 262 2271
Fragments arranged by size:
2271 915 532 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4472
SphI G_CATG'C
Cuts at: 1525 2025 1525
Size: 500 3972
StuI AGG'CCT
Cuts at: 883 883
Size: 4472
StyI C'CwwG_G
Cuts at: 107 1606 1960 107
Size: 1499 354 2619
Fragments arranged by size:
2619 1499 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 580 1153 2055 2188 2647 2856 580
Size: 573 902 133 459 209 2196
Fragments arranged by size:
2196 902 573 459 209 133
TatI w'GTAC_w
Cuts at: 3987 3987
Size: 4472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4440
Tth111I GACn'n_nGTC
Cuts at: 313 646 1301 2269 313
Size: 333 655 968 2516
Fragments arranged by size:
2516 968 655 333
XbaI T'CTAG_A
Cuts at: 1368 1503 1368
Size: 135 4337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1251 1347 1967 1251
Size: 96 620 3756
Fragments arranged by size:
3756 620 96
XmaI C'CCGG_G
Cuts at: 1492 1492
Size: 4472
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PciI
PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BmgBI BmtI BsaXI BsiWI BsmI
BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI PacI PasI PflMI PmlI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI