Restriction map for
pF1KB6669
(Circular) (Six-base) MAPSORT of: pF1KB6669.seq Check: 464 from: 1 to: 4388
pF1KB6669 4388 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1426 1426
Size: 4388
Acc65I G'GTAC_C
Cuts at: 1404 1404
Size: 4388
AclI AA'CG_TT
Cuts at: 4228 4228
Size: 4388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 809 895 1262 2213 2645 3392 3660 584
Size: 225 86 367 951 432 747 268 1312
Fragments arranged by size:
1312 951 747 432 367 268 225 86
AfeI AGC'GCT
Cuts at: 281 1175 281
Size: 894 3494
AflIII A'CryG_T
Cuts at: 520 1740 2844 3821 520
Size: 1220 1104 977 1087
Fragments arranged by size:
1220 1104 1087 977
AgeI A'CCGG_T
Cuts at: 2776 2776
Size: 4388
AhdI GACnn_n'nnGTC
Cuts at: 521 521
Size: 4388
AlwNI CAG_nnn'CTG
Cuts at: 973 1693 3260 973
Size: 720 1567 2101
Fragments arranged by size:
2101 1567 720
ApaLI G'TGCA_C
Cuts at: 1341 3158 1341
Size: 1817 2571
ApoI r'AATT_y
Cuts at: 423 1185 1392 3741 3997 423
Size: 762 207 2349 256 814
Fragments arranged by size:
2349 814 762 256 207
AseI AT'TA_AT
Cuts at: 20 20
Size: 4388
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
AvaI C'yCGr_G
Cuts at: 147 1408 147
Size: 1261 3127
BamHI G'GATC_C
Cuts at: 1413 1413
Size: 4388
BanI G'GyrC_C
Cuts at: 321 1404 1818 2066 2101 321
Size: 1083 414 248 35 2608
Fragments arranged by size:
2608 1083 414 248 35
BanII G_rGCy'C
Cuts at: 1031 1087 1402 2432 1031
Size: 56 315 1030 2987
Fragments arranged by size:
2987 1030 315 56
BbeI G_GCGC'C
Cuts at: 1822 2070 1822
Size: 248 4140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1222 1256 1222
Size: 34 4354
BciVI GTATCCnnnnn_n'
Cuts at: 2286 3047 4388 2286
Size: 761 1341 2286
Fragments arranged by size:
2286 1341 761
BclI T'GATC_A
Cuts at: 1908 1908
Size: 4388
BglI GCCn_nnn'nGGC
Cuts at: 889 889
Size: 4388
BglII A'GATC_T
Cuts at: 143 710 1903 3940 143
Size: 567 1193 2037 591
Fragments arranged by size:
2037 1193 591 567
BlpI GC'TnA_GC
Cuts at: 1500 1500
Size: 4388
Bme1580I G_kGCm'C
Cuts at: 1345 2013 2106 3162 1345
Size: 668 93 1056 2571
Fragments arranged by size:
2571 1056 668 93
BmgBI CAC'GTC
Cuts at: 1340 1340
Size: 4388
BmrI ACTGGGnnnn_n'
Cuts at: 566 1600 1681 2016 4160 566
Size: 1034 81 335 2144 794
Fragments arranged by size:
2144 1034 794 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 891 2755 891
Size: 1864 2524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 863 863
Size: 4388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1564 2443 2935 3233 853
Size: 711 879 492 298 2008
Fragments arranged by size:
2008 879 711 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1299 4092 1299
Size: 2793 1595
BsaAI yAC'GTr
Cuts at: 1049 1741 1049
Size: 692 3696
BsaBI GATnn'nnATC
Cuts at: 6 1902 3939 6
Size: 1896 2037 455
Fragments arranged by size:
2037 1896 455
BsaHI Gr'CG_yC
Cuts at: 1819 2067 2769 4265 1819
Size: 248 702 1496 1942
Fragments arranged by size:
1942 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2098 2776 3050 3197 3608 1577
Size: 521 678 274 147 411 2357
Fragments arranged by size:
2357 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 358 358
Size: 4388
BseYI C'CCAG_C
Cuts at: 1813 2571 3148 3644 1813
Size: 758 577 496 2557
Fragments arranged by size:
2557 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1190 1302 1190
Size: 112 4276
BsiEI CG_ry'CG
Cuts at: 104 1930 1976 3184 104
Size: 1826 46 1208 1308
Fragments arranged by size:
1826 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 137 1009 1066 1087 1345 1402 2180 2370 3162
Size: 872 57 21 258 57 778 190 792
Cuts at: 3162 137
Size: 1363
Fragments arranged by size:
1363 872 792 778 258 190 57 57
21
BsmI GAATG_Cn'
Cuts at: 1137 1137
Size: 4388
Bsp1286I G_dGCh'C
Cuts at: 137 1009 1031 1066 1087 1345 1402 2013 2106
Size: 872 22 35 21 258 57 611 93
Cuts at: 2106 2180 2370 2432 3162 137
Size: 74 190 62 730 1363
Fragments arranged by size:
1363 872 730 611 258 190 93 74
62 57 35 22 21
BspEI T'CCGG_A
Cuts at: 3608 3608
Size: 4388
BspHI T'CATG_A
Cuts at: 3564 3564
Size: 4388
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1438 1652 1954 2335 1438
Size: 214 302 381 3491
Fragments arranged by size:
3491 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2680 2734 4218 4383 50
Size: 2630 54 1484 165 55
Fragments arranged by size:
2630 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 666 2300 666
Size: 1634 2754
BsrFI r'CCGG_y
Cuts at: 2386 2567 2776 2386
Size: 181 209 3998
Fragments arranged by size:
3998 209 181
BsrGI T'GTAC_A
Cuts at: 213 213
Size: 4388
BssSI C'ACGA_G
Cuts at: 2659 3017 2659
Size: 358 4030
BstAPI GCAn_nnn'nTGC
Cuts at: 1765 1765
Size: 4388
BstBI TT'CG_AA
Cuts at: 2749 2749
Size: 4388
BstYI r'GATC_y
Cuts at: 1 143 710 1413 1880 1903 2238 2484 3496
Size: 142 567 703 467 23 335 246 1012
Cuts at: 3496 3582 3594 3940 1
Size: 86 12 346 449
Fragments arranged by size:
1012 703 567 467 449 346 335 246
142 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4388
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2338 2519 2682 2338
Size: 181 163 4044
Fragments arranged by size:
4044 181 163
BtsI GCAGTG_nn'
Cuts at: 135 1314 1631 135
Size: 1179 317 2892
Fragments arranged by size:
2892 1179 317
DraI TTT'AAA
Cuts at: 429 1387 429
Size: 958 3430
DraIII CAC_nnn'GTG
Cuts at: 337 337
Size: 4388
DrdI GACnn_nn'nnGTC
Cuts at: 2094 2952 2094
Size: 858 3530
EaeI y'GGCC_r
Cuts at: 1973 2147 2538 2565 3910 1973
Size: 174 391 27 1345 2451
Fragments arranged by size:
2451 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1973 1973
Size: 4388
EarI CTCTTCn'nnn_
Cuts at: 722 2411 2621 722
Size: 1689 210 2489
Fragments arranged by size:
2489 1689 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1110 2596 2906 3052 4198 1110
Size: 1486 310 146 1146 1300
Fragments arranged by size:
1486 1300 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1085 1400 1085
Size: 315 4073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 809 863 895 1262 2213 2645 3392 3660
Size: 225 54 32 367 951 432 747 268
Cuts at: 3660 584
Size: 1312
Fragments arranged by size:
1312 951 747 432 367 268 225 54
32
EcoNI CCTnn'n_nnAGG
Cuts at: 1022 1022
Size: 4388
EcoO109I rG'GnC_Cy
Cuts at: 590 1527 590
Size: 937 3451
EcoRI G'AATT_C
Cuts at: 1185 1392 1185
Size: 207 4181
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2407 2407
Size: 4388
FspI TGC'GCA
Cuts at: 2169 2169
Size: 4388
HaeII r_GCGC'y
Cuts at: 283 1177 1822 2070 3092 4071 283
Size: 894 645 248 1022 979 600
Fragments arranged by size:
1022 979 894 645 600 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 507 539 2230 2260 2262 2292 507
Size: 32 1691 30 2 30 2603
Fragments arranged by size:
2603 1691 32 30 30 2
HincII GTy'rAC
Cuts at: 1427 1427
Size: 4388
HindIII A'AGCT_T
Cuts at: 1300 1300
Size: 4388
KasI G'GCGC_C
Cuts at: 1818 2066 1818
Size: 248 4140
KpnI G_GTAC'C
Cuts at: 1408 1408
Size: 4388
MluI A'CGCG_T
Cuts at: 3821 3821
Size: 4388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 159 3059 3243 159
Size: 2900 184 1304
Fragments arranged by size:
2900 1304 184
MscI TGG'CCA
Cuts at: 2149 2149
Size: 4388
MslI CAynn'nnrTG
Cuts at: 976 2377 2504 976
Size: 1401 127 2860
Fragments arranged by size:
2860 1401 127
MspA1I CmG'CkG
Cuts at: 777 987 1499 1813 2173 3186 3431 777
Size: 210 512 314 360 1013 245 1734
Fragments arranged by size:
1734 1013 512 360 314 245 210
NaeI GCC'GGC
Cuts at: 2569 2569
Size: 4388
NarI GG'CG_CC
Cuts at: 1819 2067 1819
Size: 248 4140
NcoI C'CATG_G
Cuts at: 107 107
Size: 4388
NgoMIV G'CCGG_C
Cuts at: 2567 2567
Size: 4388
NspI r_CATG'y
Cuts at: 524 1441 1941 2848 524
Size: 917 500 907 2064
Fragments arranged by size:
2064 917 907 500
PciI A'CATG_T
Cuts at: 520 2844 520
Size: 2324 2064
PfoI T'CCnGG_A
Cuts at: 863 863
Size: 4388
PmeI GTTT'AAAC
Cuts at: 1387 1387
Size: 4388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 4356
PpuMI rG'GwC_Cy
Cuts at: 590 590
Size: 4388
PsiI TTA'TAA
Cuts at: 412 412
Size: 4388
PstI C_TGCA'G
Cuts at: 587 1435 2120 587
Size: 848 685 2855
Fragments arranged by size:
2855 848 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4388
PvuII CAG'CTG
Cuts at: 777 1813 2173 777
Size: 1036 360 2992
Fragments arranged by size:
2992 1036 360
RsrII CG'GwC_CG
Cuts at: 2583 2583
Size: 4388
SacI G_AGCT'C
Cuts at: 1087 1402 1087
Size: 315 4073
SalI G'TCGA_C
Cuts at: 1425 1425
Size: 4388
SapI GCTCTTCn'nnn_
Cuts at: 2411 2621 2411
Size: 210 4178
SbfI CC_TGCA'GG
Cuts at: 1435 1435
Size: 4388
ScaI AGT'ACT
Cuts at: 3905 3905
Size: 4388
SfcI C'TryA_G
Cuts at: 33 583 594 1431 2116 3109 3300 33
Size: 550 11 837 685 993 191 1121
Fragments arranged by size:
1121 993 837 685 550 191 11
SfoI GGC'GCC
Cuts at: 1820 2068 1820
Size: 248 4140
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4388
SmaI CCC'GGG
Cuts at: 1410 1410
Size: 4388
SmlI C'TyrA_G
Cuts at: 832 1543 2458 2950 3212 832
Size: 711 915 492 262 2008
Fragments arranged by size:
2008 915 711 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4388
SphI G_CATG'C
Cuts at: 1441 1941 1441
Size: 500 3888
SspI AAT'ATT
Cuts at: 763 763
Size: 4388
StyI C'CwwG_G
Cuts at: 107 1088 1522 1876 107
Size: 981 434 354 2619
Fragments arranged by size:
2619 981 434 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 963 1049 1971 2104 2563 2772 963
Size: 86 922 133 459 209 2579
Fragments arranged by size:
2579 922 459 209 133 86
TatI w'GTAC_w
Cuts at: 213 3903 213
Size: 3690 698
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1335 1367 3905 3937 1335
Size: 32 2538 32 1786
Fragments arranged by size:
2538 1786 32 32
Tth111I GACn'n_nGTC
Cuts at: 2185 2185
Size: 4388
XbaI T'CTAG_A
Cuts at: 1419 1419
Size: 4388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1883 1883
Size: 4388
XmaI C'CCGG_G
Cuts at: 1408 1408
Size: 4388
XmnI GAAnn'nnTTC
Cuts at: 908 908
Size: 4388
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PsiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmtI Bpu10I BsaXI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI