Restriction map for
pF1KB6672
(Circular) (Six-base) MAPSORT of: pF1KB6672.seq Check: 2837 from: 1 to: 4475
pF1KB6672 4475 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 2300 2732 3479 3747 410
Size: 1890 432 747 268 1138
Fragments arranged by size:
1890 1138 747 432 268
AflII C'TTAA_G
Cuts at: 130 130
Size: 4475
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 694 1320 1780 3347 694
Size: 626 460 1567 1822
Fragments arranged by size:
1822 1567 626 460
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 269 643 665 697 1479 3828 4084 269
Size: 374 22 32 782 2349 256 660
Fragments arranged by size:
2349 782 660 374 256 32 22
AseI AT'TA_AT
Cuts at: 20 890 20
Size: 870 3605
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 1495 1495
Size: 4475
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 1491 1905 2153 2188 1491
Size: 414 248 35 3778
Fragments arranged by size:
3778 414 248 35
BanII G_rGCy'C
Cuts at: 1489 2519 1489
Size: 1030 3445
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 237 984 237
Size: 747 3728
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4441
BciVI GTATCCnnnnn_n'
Cuts at: 445 485 1228 2373 3134 4475 445
Size: 40 743 1145 761 1341 445
Fragments arranged by size:
1341 1145 761 743 445 40
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BfrBI ATG'CAT
Cuts at: 111 111
Size: 4475
BglII A'GATC_T
Cuts at: 798 1990 4027 798
Size: 1192 2037 1246
Fragments arranged by size:
2037 1246 1192
BlpI GC'TnA_GC
Cuts at: 1587 1587
Size: 4475
Bme1580I G_kGCm'C
Cuts at: 2100 2193 3249 2100
Size: 93 1056 3326
Fragments arranged by size:
3326 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 545 2842 545
Size: 2297 2178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 198 1098 174
Size: 24 900 3551
Fragments arranged by size:
3551 900 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1651 2530 3022 3320 1651
Size: 879 492 298 2806
Fragments arranged by size:
2806 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 424 4179 424
Size: 3755 720
BsaAI yAC'GTr
Cuts at: 1347 1828 1347
Size: 481 3994
BsaBI GATnn'nnATC
Cuts at: 6 797 1989 4026 6
Size: 791 1192 2037 455
Fragments arranged by size:
2037 1192 791 455
BsaHI Gr'CG_yC
Cuts at: 1906 2154 2856 4352 1906
Size: 248 702 1496 2029
Fragments arranged by size:
2029 1496 702 248
BsaWI w'CCGG_w
Cuts at: 701 716 1664 2185 2863 3137 3284 3695 701
Size: 15 948 521 678 274 147 411 1481
Fragments arranged by size:
1481 948 678 521 411 274 147 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 668 698 668
Size: 30 4445
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 497 518 1101 497
Size: 21 583 3871
Fragments arranged by size:
3871 583 21
BseYI C'CCAG_C
Cuts at: 161 456 1900 2658 3235 3731 161
Size: 295 1444 758 577 496 905
Fragments arranged by size:
1444 905 758 577 496 295
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 944 320
Size: 624 3851
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1368 1489 2267 2457 3249 1368
Size: 121 778 190 792 2594
Fragments arranged by size:
2594 792 778 190 121
BsmI GAATG_Cn'
Cuts at: 1296 1296
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 1368 1489 2100 2193 2267 2457 2519 3249 1368
Size: 121 611 93 74 190 62 730 2594
Fragments arranged by size:
2594 730 611 190 121 93 74 62
BspEI T'CCGG_A
Cuts at: 701 3695 701
Size: 2994 1481
BspHI T'CATG_A
Cuts at: 578 3651 578
Size: 3073 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1525 1739 2041 2422 1525
Size: 214 302 381 3578
Fragments arranged by size:
3578 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2767 2821 4305 4470 50
Size: 2717 54 1484 165 55
Fragments arranged by size:
2717 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BssSI C'ACGA_G
Cuts at: 2746 3104 2746
Size: 358 4117
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 354 798 1197 1500 1967 1990 2325 2571
Size: 353 444 399 303 467 23 335 246
Cuts at: 2571 3583 3669 3681 4027 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 444 399 353 346 335
303 246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 689 1718 689
Size: 1029 3446
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 2060 2234 2625 2652 3997 2060
Size: 174 391 27 1345 2538
Fragments arranged by size:
2538 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 386 422 2498 2708 386
Size: 36 2076 210 2153
Fragments arranged by size:
2153 2076 210 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2683 2993 3139 4285 2683
Size: 310 146 1146 2873
Fragments arranged by size:
2873 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1487 1487
Size: 4475
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 198 410 1098 2300 2732 3479 3747 174
Size: 24 212 688 1202 432 747 268 902
Fragments arranged by size:
1202 902 747 688 432 268 212 24
EcoO109I rG'GnC_Cy
Cuts at: 985 1614 985
Size: 629 3846
EcoRI G'AATT_C
Cuts at: 697 1479 697
Size: 782 3693
EcoRV GAT'ATC
Cuts at: 861 861
Size: 4475
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1909 2157 3179 4158 1909
Size: 248 1022 979 2226
Fragments arranged by size:
2226 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 492 524 861 893 932 964 1396 1428 2317
Size: 32 337 32 39 32 432 32 889
Cuts at: 2317 2347 2349 2379 492
Size: 30 2 30 2588
Fragments arranged by size:
2588 889 432 337 39 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 591 1514 591
Size: 923 3552
HindIII A'AGCT_T
Cuts at: 830 830
Size: 4475
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 377 1110 1393 3146 3330 377
Size: 733 283 1753 184 1522
Fragments arranged by size:
1753 1522 733 283 184
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 577 1289 2464 2591 577
Size: 712 1175 127 2461
Fragments arranged by size:
2461 1175 712 127
MspA1I CmG'CkG
Cuts at: 694 1586 1900 2260 3273 3518 694
Size: 892 314 360 1013 245 1651
Fragments arranged by size:
1651 1013 892 360 314 245
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NdeI CA'TA_TG
Cuts at: 1286 1286
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NsiI A_TGCA'T
Cuts at: 113 113
Size: 4475
NspI r_CATG'y
Cuts at: 1528 2028 2935 1528
Size: 500 907 3068
Fragments arranged by size:
3068 907 500
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PfoI T'CCnGG_A
Cuts at: 1193 1193
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 985 985
Size: 4475
PshAI GACnn'nnGTC
Cuts at: 990 990
Size: 4475
PsiI TTA'TAA
Cuts at: 1139 1139
Size: 4475
PstI C_TGCA'G
Cuts at: 1522 2207 1522
Size: 685 3790
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 694 1900 2260 694
Size: 1206 360 2909
Fragments arranged by size:
2909 1206 360
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 1489 1489
Size: 4475
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 2498 2708 2498
Size: 210 4265
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 403 3992 403
Size: 3589 886
SfcI C'TryA_G
Cuts at: 33 1307 1518 2203 3196 3387 33
Size: 1274 211 685 993 191 1121
Fragments arranged by size:
1274 1121 993 685 211 191
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 130 1630 2545 3037 3299 130
Size: 1500 915 492 262 1306
Fragments arranged by size:
1500 1306 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1528 2028 1528
Size: 500 3975
StyI C'CwwG_G
Cuts at: 419 1609 1963 419
Size: 1190 354 2931
Fragments arranged by size:
2931 1190 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 532 873 2058 2191 2650 2859 532
Size: 341 1185 133 459 209 2148
Fragments arranged by size:
2148 1185 459 341 209 133
TatI w'GTAC_w
Cuts at: 401 1397 3990 401
Size: 996 2593 886
Fragments arranged by size:
2593 996 886
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 507 539 668 700 3992 4024 507
Size: 32 129 32 3292 32 958
Fragments arranged by size:
3292 958 129 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 574 2272 574
Size: 1698 2777
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 670 1970 670
Size: 1300 3175
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PciI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NcoI NheI NotI NruI PacI PasI PflMI
PmlI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI