Restriction map for
pF1KB6674
(Circular) (Six-base) MAPSORT of: pF1KB6674.seq Check: 7206 from: 1 to: 4475
pF1KB6674 4475 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 893 893
Size: 4475
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2300 2732 3479 3747 2300
Size: 432 747 268 3028
Fragments arranged by size:
3028 747 432 268
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 192 711 1780 3347 192
Size: 519 1069 1567 1320
Fragments arranged by size:
1567 1320 1069 519
ApaI G_GGCC'C
Cuts at: 1309 1379 1309
Size: 70 4405
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 1174 1479 3828 4084 1174
Size: 305 2349 256 1565
Fragments arranged by size:
2349 1565 305 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 408 664 1495 408
Size: 256 831 3388
Fragments arranged by size:
3388 831 256
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 1491 1905 2153 2188 1491
Size: 414 248 35 3778
Fragments arranged by size:
3778 414 248 35
BanII G_rGCy'C
Cuts at: 415 704 1309 1379 1489 2519 415
Size: 289 605 70 110 1030 2371
Fragments arranged by size:
2371 1030 605 289 110 70
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 105 105
Size: 4475
BbvCI CC'TCA_GC
Cuts at: 1094 1094
Size: 4475
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 817 851 817
Size: 34 4441
BciVI GTATCCnnnnn_n'
Cuts at: 1128 2373 3134 4475 1128
Size: 1245 761 1341 1128
Fragments arranged by size:
1341 1245 1128 761
BclI T'GATC_A
Cuts at: 518 1995 518
Size: 1477 2998
BglI GCCn_nnn'nGGC
Cuts at: 359 1383 359
Size: 1024 3451
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 319 1587 319
Size: 1268 3207
Bme1580I G_kGCm'C
Cuts at: 1309 1379 2100 2193 3249 1309
Size: 70 721 93 1056 2535
Fragments arranged by size:
2535 1056 721 93 70
BmrI ACTGGGnnnn_n'
Cuts at: 689 861 975 1303 1311 1687 1768 2103 4247
Size: 172 114 328 8 376 81 335 2144
Cuts at: 4247 689
Size: 917
Fragments arranged by size:
2144 917 376 335 328 172 114 81
8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1221 2842 1221
Size: 1621 2854
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 531 610 688 1256 253
Size: 278 79 78 568 3472
Fragments arranged by size:
3472 568 278 79 78
Bpu10I CC'TnA_GC
Cuts at: 1094 1094
Size: 4475
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 1651 2530 3022 3320 490
Size: 1161 879 492 298 1645
Fragments arranged by size:
1645 1161 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4179 4179
Size: 4475
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 1989 4026 6
Size: 1983 2037 455
Fragments arranged by size:
2037 1983 455
BsaHI Gr'CG_yC
Cuts at: 908 1906 2154 2856 4352 908
Size: 998 248 702 1496 1031
Fragments arranged by size:
1496 1031 998 702 248
BsaWI w'CCGG_w
Cuts at: 1664 2185 2863 3137 3284 3695 1664
Size: 521 678 274 147 411 2444
Fragments arranged by size:
2444 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 583 613 583
Size: 30 4445
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 145 1055 145
Size: 910 3565
BseYI C'CCAG_C
Cuts at: 296 346 357 1900 2658 3235 3731 296
Size: 50 11 1543 758 577 496 1040
Fragments arranged by size:
1543 1040 758 577 496 50 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 352 352
Size: 4475
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 263 704 1028 1489 2267 2457 3249 263
Size: 441 324 461 778 190 792 1489
Fragments arranged by size:
1489 792 778 461 441 324 190
Bsp1286I G_dGCh'C
Cuts at: 263 415 704 1028 1309 1379 1489 2100 2193
Size: 152 289 324 281 70 110 611 93
Cuts at: 2193 2267 2457 2519 3249 263
Size: 74 190 62 730 1489
Fragments arranged by size:
1489 730 611 324 289 281 190 152
110 93 74 70 62
BspEI T'CCGG_A
Cuts at: 3695 3695
Size: 4475
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 510 893 1525 1739 2041 2422 510
Size: 383 632 214 302 381 2563
Fragments arranged by size:
2563 632 383 381 302 214
BsrBI CCG'CTC
Cuts at: 50 594 2767 2821 4305 4470 50
Size: 544 2173 54 1484 165 55
Fragments arranged by size:
2173 1484 544 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BsrGI T'GTAC_A
Cuts at: 245 245
Size: 4475
BssSI C'ACGA_G
Cuts at: 449 2746 3104 449
Size: 2297 358 1820
Fragments arranged by size:
2297 1820 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 540 1500 1967 1990 2325 2571 3583 3669
Size: 539 960 467 23 335 246 1012 86
Cuts at: 3669 3681 4027 1
Size: 12 346 449
Fragments arranged by size:
1012 960 539 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 952 1290 107
Size: 845 338 3292
Fragments arranged by size:
3292 845 338
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 670 2425 2606 2769 670
Size: 1755 181 163 2376
Fragments arranged by size:
2376 1755 181 163
BtsI GCAGTG_nn'
Cuts at: 280 777 1718 280
Size: 497 941 3037
Fragments arranged by size:
3037 941 497
DraI TTT'AAA
Cuts at: 1474 1474
Size: 4475
DraIII CAC_nnn'GTG
Cuts at: 429 429
Size: 4475
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 417 767 2060 2234 2625 2652 3997 417
Size: 350 1293 174 391 27 1345 895
Fragments arranged by size:
1345 1293 895 391 350 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 479 1120 2498 2708 479
Size: 641 1378 210 2246
Fragments arranged by size:
2246 1378 641 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 385 2683 2993 3139 4285 385
Size: 2298 310 146 1146 575
Fragments arranged by size:
2298 1146 575 310 146
EcoICRI GAG'CTC
Cuts at: 702 1487 702
Size: 785 3690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 531 610 688 1256 2300 2732 3479 3747
Size: 278 79 78 568 1044 432 747 268
Cuts at: 3747 253
Size: 981
Fragments arranged by size:
1044 981 747 568 432 278 268 79
78
EcoNI CCTnn'n_nnAGG
Cuts at: 1129 1129
Size: 4475
EcoO109I rG'GnC_Cy
Cuts at: 252 1305 1375 1376 1614 252
Size: 1053 70 1 238 3113
Fragments arranged by size:
3113 1053 238 70 1
EcoRI G'AATT_C
Cuts at: 1174 1479 1174
Size: 305 4170
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 607 2256 607
Size: 1649 2826
FspAI rTGC'GCAy
Cuts at: 607 607
Size: 4475
HaeII r_GCGC'y
Cuts at: 1909 2157 3179 4158 1909
Size: 248 1022 979 2226
Fragments arranged by size:
2226 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 369 401 966 998 2317 2347 2349 2379 369
Size: 32 565 32 1319 30 2 30 2465
Fragments arranged by size:
2465 1319 565 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3146 3330 3146
Size: 184 4291
MscI TGG'CCA
Cuts at: 419 2236 419
Size: 1817 2658
MslI CAynn'nnrTG
Cuts at: 655 839 2464 2591 655
Size: 184 1625 127 2539
Fragments arranged by size:
2539 1625 184 127
MspA1I CmG'CkG
Cuts at: 147 192 292 300 339 357 1389 1586 1900
Size: 45 100 8 39 18 1032 197 314
Cuts at: 1900 2260 3273 3518 147
Size: 360 1013 245 1104
Fragments arranged by size:
1104 1032 1013 360 314 245 197 100
45 39 18 8
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NcoI C'CATG_G
Cuts at: 107 1290 107
Size: 1183 3292
NdeI CA'TA_TG
Cuts at: 495 495
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NspI r_CATG'y
Cuts at: 820 865 1528 2028 2935 820
Size: 45 663 500 907 2360
Fragments arranged by size:
2360 907 663 500 45
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PflMI CCAn_nnn'nTGG
Cuts at: 342 711 1276 342
Size: 369 565 3541
Fragments arranged by size:
3541 565 369
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PpuMI rG'GwC_Cy
Cuts at: 252 252
Size: 4475
PspOMI G'GGCC_C
Cuts at: 1305 1375 1305
Size: 70 4405
PstI C_TGCA'G
Cuts at: 1384 1522 2207 1384
Size: 138 685 3652
Fragments arranged by size:
3652 685 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 192 292 300 339 357 1900 2260 192
Size: 100 8 39 18 1543 360 2407
Fragments arranged by size:
2407 1543 360 100 39 18 8
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 704 1489 704
Size: 785 3690
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 479 2498 2708 479
Size: 2019 210 2246
Fragments arranged by size:
2246 2019 210
SbfI CC_TGCA'GG
Cuts at: 1384 1522 1384
Size: 138 4337
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SexAI A'CCwGG_T
Cuts at: 254 512 254
Size: 258 4217
SfcI C'TryA_G
Cuts at: 33 753 1380 1518 2203 3196 3387 33
Size: 720 627 138 685 993 191 1121
Fragments arranged by size:
1121 993 720 685 627 191 138
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 469 1630 2545 3037 3299 469
Size: 1161 915 492 262 1645
Fragments arranged by size:
1645 1161 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 820 865 1528 2028 820
Size: 45 663 500 3267
Fragments arranged by size:
3267 663 500 45
StuI AGG'CCT
Cuts at: 175 175
Size: 4475
StyI C'CwwG_G
Cuts at: 107 308 1290 1609 1963 107
Size: 201 982 319 354 2619
Fragments arranged by size:
2619 982 354 319 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2058 2191 2650 2859 2058
Size: 133 459 209 3674
Fragments arranged by size:
3674 459 209 133
TatI w'GTAC_w
Cuts at: 245 822 3990 245
Size: 577 3168 730
Fragments arranged by size:
3168 730 577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1256 1288 3992 4024 1256
Size: 32 2704 32 1707
Fragments arranged by size:
2704 1707 32 32
Tth111I GACn'n_nGTC
Cuts at: 160 2272 160
Size: 2112 2363
XbaI T'CTAG_A
Cuts at: 1506 1506
Size: 4475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 343 1225 1970 343
Size: 882 745 2848
Fragments arranged by size:
2848 882 745
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV
FseI HindIII HpaI MfeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI