Restriction map for
pF1KB6676
(Circular) (Six-base) MAPSORT of: pF1KB6676.seq Check: 662 from: 1 to: 4475
pF1KB6676 4475 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1513 1513
Size: 4475
Acc65I G'GTAC_C
Cuts at: 1491 1491
Size: 4475
AclI AA'CG_TT
Cuts at: 4315 4315
Size: 4475
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 2300 2732 3479 3747 482
Size: 1818 432 747 268 1210
Fragments arranged by size:
1818 1210 747 432 268
AflIII A'CryG_T
Cuts at: 1827 2931 3908 1827
Size: 1104 977 2394
Fragments arranged by size:
2394 1104 977
AgeI A'CCGG_T
Cuts at: 2863 2863
Size: 4475
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1219 1219
Size: 4475
AlwNI CAG_nnn'CTG
Cuts at: 123 186 1780 3347 123
Size: 63 1594 1567 1251
Fragments arranged by size:
1594 1567 1251 63
ApaI G_GGCC'C
Cuts at: 414 900 414
Size: 486 3989
ApaLI G'TGCA_C
Cuts at: 3245 3245
Size: 4475
ApoI r'AATT_y
Cuts at: 466 1157 1479 3828 4084 466
Size: 691 322 2349 256 857
Fragments arranged by size:
2349 857 691 322 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4475
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
AvaI C'yCGr_G
Cuts at: 892 1495 892
Size: 603 3872
BamHI G'GATC_C
Cuts at: 1500 1500
Size: 4475
BanI G'GyrC_C
Cuts at: 1491 1905 2153 2188 1491
Size: 414 248 35 3778
Fragments arranged by size:
3778 414 248 35
BanII G_rGCy'C
Cuts at: 164 414 446 900 1412 1489 2519 164
Size: 250 32 454 512 77 1030 2120
Fragments arranged by size:
2120 1030 512 454 250 77 32
BbeI G_GCGC'C
Cuts at: 1909 2157 1909
Size: 248 4227
BbsI GAAGACnn'nnnn_
Cuts at: 797 878 797
Size: 81 4394
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1285 1319 90
Size: 34 1161 34 3246
Fragments arranged by size:
3246 1161 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2373 3134 4475 2373
Size: 761 1341 2373
Fragments arranged by size:
2373 1341 761
BclI T'GATC_A
Cuts at: 1995 1995
Size: 4475
BfrBI ATG'CAT
Cuts at: 691 691
Size: 4475
BglI GCCn_nnn'nGGC
Cuts at: 1354 1354
Size: 4475
BglII A'GATC_T
Cuts at: 1990 4027 1990
Size: 2037 2438
BlpI GC'TnA_GC
Cuts at: 163 1587 163
Size: 1424 3051
Bme1580I G_kGCm'C
Cuts at: 414 900 2100 2193 3249 414
Size: 486 1200 93 1056 1640
Fragments arranged by size:
1640 1200 1056 486 93
BmrI ACTGGGnnnn_n'
Cuts at: 1687 1768 2103 4247 1687
Size: 81 335 2144 1915
Fragments arranged by size:
2144 1915 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2842 2842
Size: 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 612 1477 592
Size: 20 865 3590
Fragments arranged by size:
3590 865 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1651 2530 3022 3320 1153
Size: 498 879 492 298 2308
Fragments arranged by size:
2308 879 498 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4179 4179
Size: 4475
BsaAI yAC'GTr
Cuts at: 1828 1828
Size: 4475
BsaBI GATnn'nnATC
Cuts at: 6 146 1989 4026 6
Size: 140 1843 2037 455
Fragments arranged by size:
2037 1843 455 140
BsaHI Gr'CG_yC
Cuts at: 1310 1906 2154 2856 4352 1310
Size: 596 248 702 1496 1433
Fragments arranged by size:
1496 1433 702 596 248
BsaWI w'CCGG_w
Cuts at: 516 1076 1664 2185 2863 3137 3284 3695 516
Size: 560 588 521 678 274 147 411 1296
Fragments arranged by size:
1296 678 588 560 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1105 1105
Size: 4475
BseYI C'CCAG_C
Cuts at: 1900 2658 3235 3731 1900
Size: 758 577 496 2644
Fragments arranged by size:
2644 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2017 2063 3271 104
Size: 1913 46 1208 1308
Fragments arranged by size:
1913 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 164 386 1198 1412 1489 2267 2457 3249 164
Size: 222 812 214 77 778 190 792 1390
Fragments arranged by size:
1390 812 792 778 222 214 190 77
BsmBI CGTCTCn'nnnn_
Cuts at: 837 837
Size: 4475
Bsp1286I G_dGCh'C
Cuts at: 164 386 414 446 900 1198 1412 1489 2100
Size: 222 28 32 454 298 214 77 611
Cuts at: 2100 2193 2267 2457 2519 3249 164
Size: 93 74 190 62 730 1390
Fragments arranged by size:
1390 730 611 454 298 222 214 190
93 77 74 62 32 28
BspEI T'CCGG_A
Cuts at: 516 3695 516
Size: 3179 1296
BspHI T'CATG_A
Cuts at: 3651 3651
Size: 4475
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1525 1739 2041 2422 1525
Size: 214 302 381 3578
Fragments arranged by size:
3578 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1111 2767 2821 4305 4470 50
Size: 1061 1656 54 1484 165 55
Fragments arranged by size:
1656 1484 1061 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2387 2387
Size: 4475
BsrFI r'CCGG_y
Cuts at: 2473 2654 2863 2473
Size: 181 209 4085
Fragments arranged by size:
4085 209 181
BsrGI T'GTAC_A
Cuts at: 134 134
Size: 4475
BssSI C'ACGA_G
Cuts at: 866 2746 3104 866
Size: 1880 358 2237
Fragments arranged by size:
2237 1880 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1852 1852
Size: 4475
BstBI TT'CG_AA
Cuts at: 2836 2836
Size: 4475
BstYI r'GATC_y
Cuts at: 1 141 182 212 1500 1967 1990 2325 2571
Size: 140 41 30 1288 467 23 335 246
Cuts at: 2571 3583 3669 3681 4027 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1288 1012 467 449 346 335 246 140
86 41 30 23 12
BtgI C'CryG_G
Cuts at: 1152 1152
Size: 4475
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2425 2606 2769 2425
Size: 181 163 4131
Fragments arranged by size:
4131 181 163
BtsI GCAGTG_nn'
Cuts at: 1718 1718
Size: 4475
ClaI AT'CG_AT
Cuts at: 1295 1295
Size: 4475
DraI TTT'AAA
Cuts at: 1086 1474 1086
Size: 388 4087
DrdI GACnn_nn'nnGTC
Cuts at: 2181 3039 2181
Size: 858 3617
EaeI y'GGCC_r
Cuts at: 2060 2234 2625 2652 3997 2060
Size: 174 391 27 1345 2538
Fragments arranged by size:
2538 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2060 2060
Size: 4475
EarI CTCTTCn'nnn_
Cuts at: 434 602 749 835 903 2498 2708 434
Size: 168 147 86 68 1595 210 2201
Fragments arranged by size:
2201 1595 210 168 147 86 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1372 2683 2993 3139 4285 1372
Size: 1311 310 146 1146 1562
Fragments arranged by size:
1562 1311 1146 310 146
EcoICRI GAG'CTC
Cuts at: 162 1410 1487 162
Size: 1248 77 3150
Fragments arranged by size:
3150 1248 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 592 612 1477 2300 2732 3479 3747 482
Size: 110 20 865 823 432 747 268 1210
Fragments arranged by size:
1210 865 823 747 432 268 110 20
EcoNI CCTnn'n_nnAGG
Cuts at: 1402 1402
Size: 4475
EcoO109I rG'GnC_Cy
Cuts at: 410 896 1614 410
Size: 486 718 3271
Fragments arranged by size:
3271 718 486
EcoRI G'AATT_C
Cuts at: 466 1157 1479 466
Size: 691 322 3462
Fragments arranged by size:
3462 691 322
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 4475
FspI TGC'GCA
Cuts at: 2256 2256
Size: 4475
HaeII r_GCGC'y
Cuts at: 1909 2157 3179 4158 1909
Size: 248 1022 979 2226
Fragments arranged by size:
2226 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 565 597 2317 2347 2349 2379 565
Size: 32 1720 30 2 30 2661
Fragments arranged by size:
2661 1720 32 30 30 2
HincII GTy'rAC
Cuts at: 1514 1514
Size: 4475
KasI G'GCGC_C
Cuts at: 1905 2153 1905
Size: 248 4227
KpnI G_GTAC'C
Cuts at: 1495 1495
Size: 4475
MluI A'CGCG_T
Cuts at: 3908 3908
Size: 4475
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 107 240 1247 3146 3330 107
Size: 133 1007 1899 184 1252
Fragments arranged by size:
1899 1252 1007 184 133
MscI TGG'CCA
Cuts at: 2236 2236
Size: 4475
MslI CAynn'nnrTG
Cuts at: 1201 2464 2591 1201
Size: 1263 127 3085
Fragments arranged by size:
3085 1263 127
MspA1I CmG'CkG
Cuts at: 832 1443 1586 1900 2260 3273 3518 832
Size: 611 143 314 360 1013 245 1789
Fragments arranged by size:
1789 1013 611 360 314 245 143
NaeI GCC'GGC
Cuts at: 2656 2656
Size: 4475
NarI GG'CG_CC
Cuts at: 1906 2154 1906
Size: 248 4227
NcoI C'CATG_G
Cuts at: 1152 1152
Size: 4475
NdeI CA'TA_TG
Cuts at: 687 687
Size: 4475
NgoMIV G'CCGG_C
Cuts at: 2654 2654
Size: 4475
NsiI A_TGCA'T
Cuts at: 693 693
Size: 4475
NspI r_CATG'y
Cuts at: 1206 1348 1528 2028 2935 1206
Size: 142 180 500 907 2746
Fragments arranged by size:
2746 907 500 180 142
PasI CC'CwG_GG
Cuts at: 1398 1398
Size: 4475
PciI A'CATG_T
Cuts at: 2931 2931
Size: 4475
PmeI GTTT'AAAC
Cuts at: 1474 1474
Size: 4475
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3614 3646 3614
Size: 32 4443
PspOMI G'GGCC_C
Cuts at: 410 896 410
Size: 486 3989
PstI C_TGCA'G
Cuts at: 512 1308 1522 2207 512
Size: 796 214 685 2780
Fragments arranged by size:
2780 796 685 214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4475
PvuII CAG'CTG
Cuts at: 832 1443 1900 2260 832
Size: 611 457 360 3047
Fragments arranged by size:
3047 611 457 360
RsrII CG'GwC_CG
Cuts at: 2670 2670
Size: 4475
SacI G_AGCT'C
Cuts at: 164 1412 1489 164
Size: 1248 77 3150
Fragments arranged by size:
3150 1248 77
SalI G'TCGA_C
Cuts at: 1512 1512
Size: 4475
SapI GCTCTTCn'nnn_
Cuts at: 434 2498 2708 434
Size: 2064 210 2201
Fragments arranged by size:
2201 2064 210
SbfI CC_TGCA'GG
Cuts at: 1522 1522
Size: 4475
ScaI AGT'ACT
Cuts at: 3992 3992
Size: 4475
SfcI C'TryA_G
Cuts at: 33 508 1304 1314 1339 1518 2203 3196 3387
Size: 475 796 10 25 179 685 993 191
Cuts at: 3387 33
Size: 1121
Fragments arranged by size:
1121 993 796 685 475 191 179 25
10
SfoI GGC'GCC
Cuts at: 1907 2155 1907
Size: 248 4227
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4475
SmaI CCC'GGG
Cuts at: 1497 1497
Size: 4475
SmlI C'TyrA_G
Cuts at: 1168 1630 2545 3037 3299 1168
Size: 462 915 492 262 2344
Fragments arranged by size:
2344 915 492 462 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4475
SphI G_CATG'C
Cuts at: 1206 1348 1528 2028 1206
Size: 142 180 500 3653
Fragments arranged by size:
3653 500 180 142
SspI AAT'ATT
Cuts at: 645 645
Size: 4475
StyI C'CwwG_G
Cuts at: 851 1152 1352 1609 1963 851
Size: 301 200 257 354 3363
Fragments arranged by size:
3363 354 301 257 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 333 990 2058 2191 2650 2859 333
Size: 657 1068 133 459 209 1949
Fragments arranged by size:
1949 1068 657 459 209 133
TatI w'GTAC_w
Cuts at: 134 3990 134
Size: 3856 619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3992 4024 3992
Size: 32 4443
Tth111I GACn'n_nGTC
Cuts at: 1047 2272 1047
Size: 1225 3250
XbaI T'CTAG_A
Cuts at: 363 1506 363
Size: 1143 3332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1395 1970 1395
Size: 575 3900
XmaI C'CCGG_G
Cuts at: 1495 1495
Size: 4475
XmnI GAAnn'nnTTC
Cuts at: 1187 1187
Size: 4475
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbvCI BmgBI BmtI Bpu10I BsaXI BsgI
BsiWI BsmI BssHII BstEII BstXI BstZ17I Bsu36I CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PflMI PfoI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI