Restriction map for
pF1KB6677
(Circular) (Six-base) MAPSORT of: pF1KB6677.seq Check: 3152 from: 1 to: 4394
pF1KB6677 4394 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 786 1410 786
Size: 624 3770
AclI AA'CG_TT
Cuts at: 1388 4234 1388
Size: 2846 1548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 785 2219 2651 3398 3666 320
Size: 465 1434 432 747 268 1048
Fragments arranged by size:
1434 1048 747 465 432 268
AflII C'TTAA_G
Cuts at: 1000 1000
Size: 4394
AflIII A'CryG_T
Cuts at: 1746 2850 3827 1746
Size: 1104 977 2313
Fragments arranged by size:
2313 1104 977
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 915 3164 915
Size: 2249 2145
ApoI r'AATT_y
Cuts at: 844 1188 1231 1398 3747 4003 844
Size: 344 43 167 2349 256 1235
Fragments arranged by size:
2349 1235 344 256 167 43
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1414 1414
Size: 4394
BamHI G'GATC_C
Cuts at: 737 1419 737
Size: 682 3712
BanI G'GyrC_C
Cuts at: 786 1410 1824 2072 2107 786
Size: 624 414 248 35 3073
Fragments arranged by size:
3073 624 414 248 35
BanII G_rGCy'C
Cuts at: 242 1408 2438 242
Size: 1166 1030 2198
Fragments arranged by size:
2198 1166 1030
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 552 1914 552
Size: 1362 3032
BglII A'GATC_T
Cuts at: 1909 3946 1909
Size: 2037 2357
BlpI GC'TnA_GC
Cuts at: 241 1506 241
Size: 1265 3129
Bme1580I G_kGCm'C
Cuts at: 919 2019 2112 3168 919
Size: 1100 93 1056 2145
Fragments arranged by size:
2145 1100 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2761 2761
Size: 4394
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 696 696
Size: 4394
Bpu10I CC'TnA_GC
Cuts at: 412 412
Size: 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 1570 2449 2941 3239 996
Size: 574 879 492 298 2151
Fragments arranged by size:
2151 879 574 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 476 4098 476
Size: 3622 772
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 1825 2073 2775 4271 1825
Size: 248 702 1496 1948
Fragments arranged by size:
1948 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1361 1583 2104 2782 3056 3203 3614 1361
Size: 222 521 678 274 147 411 2141
Fragments arranged by size:
2141 678 521 411 274 222 147
BseYI C'CCAG_C
Cuts at: 286 535 1819 2577 3154 3650 286
Size: 249 1284 758 577 496 1030
Fragments arranged by size:
1284 1030 758 577 496 249
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 242 919 1408 2186 2376 3168 242
Size: 677 489 778 190 792 1468
Fragments arranged by size:
1468 792 778 677 489 190
BsmI GAATG_Cn'
Cuts at: 405 405
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 242 919 1408 2019 2112 2186 2376 2438 3168
Size: 677 489 611 93 74 190 62 730
Cuts at: 3168 242
Size: 1468
Fragments arranged by size:
1468 730 677 611 489 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 938 3570 938
Size: 2632 1762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1444 1658 1960 2341 1444
Size: 214 302 381 3497
Fragments arranged by size:
3497 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 297 2306 297
Size: 2009 2385
BsrFI r'CCGG_y
Cuts at: 2392 2573 2782 2392
Size: 181 209 4004
Fragments arranged by size:
4004 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1771 1771
Size: 4394
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstEII G'GTnAC_C
Cuts at: 252 252
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 276 276
Size: 4394
BstYI r'GATC_y
Cuts at: 1 737 1419 1886 1909 2244 2490 3502 3588
Size: 736 682 467 23 335 246 1012 86
Cuts at: 3588 3600 3946 1
Size: 12 346 449
Fragments arranged by size:
1012 736 682 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 124 124
Size: 4394
BtgI C'CryG_G
Cuts at: 107 107
Size: 4394
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 917 1637 917
Size: 720 3674
DraI TTT'AAA
Cuts at: 1236 1393 1236
Size: 157 4237
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 755 1979 2153 2544 2571 3916 755
Size: 1224 174 391 27 1345 1233
Fragments arranged by size:
1345 1233 1224 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 889 1076 2417 2627 889
Size: 187 1341 210 2656
Fragments arranged by size:
2656 1341 210 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 240 1406 240
Size: 1166 3228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 320 696 785 2219 2651 3398 3666 320
Size: 376 89 1434 432 747 268 1048
Fragments arranged by size:
1434 1048 747 432 376 268 89
EcoO109I rG'GnC_Cy
Cuts at: 1533 1533
Size: 4394
EcoRI G'AATT_C
Cuts at: 1188 1398 1188
Size: 210 4184
EcoRV GAT'ATC
Cuts at: 365 365
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2413 2413
Size: 4394
FspI TGC'GCA
Cuts at: 2175 2175
Size: 4394
HaeII r_GCGC'y
Cuts at: 543 1828 2076 3098 4077 543
Size: 1285 248 1022 979 860
Fragments arranged by size:
1285 1022 979 860 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 146 178 453 473 485 505 2236 2266 2268
Size: 32 275 20 12 20 1731 30 2
Cuts at: 2268 2298 146
Size: 30 2242
Fragments arranged by size:
2242 1731 275 32 30 30 20 20
12 2
HincII GTy'rAC
Cuts at: 651 1433 651
Size: 782 3612
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 790 1414 790
Size: 624 3770
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 132 3065 3249 132
Size: 2933 184 1277
Fragments arranged by size:
2933 1277 184
MscI TGG'CCA
Cuts at: 757 2155 757
Size: 1398 2996
MslI CAynn'nnrTG
Cuts at: 209 937 2383 2510 209
Size: 728 1446 127 2093
Fragments arranged by size:
2093 1446 728 127
MspA1I CmG'CkG
Cuts at: 1505 1819 2179 3192 3437 1505
Size: 314 360 1013 245 2462
Fragments arranged by size:
2462 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2575 2575
Size: 4394
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 107
Size: 4394
NgoMIV G'CCGG_C
Cuts at: 2573 2573
Size: 4394
NspI r_CATG'y
Cuts at: 1447 1947 2854 1447
Size: 500 907 2987
Fragments arranged by size:
2987 907 500
PciI A'CATG_T
Cuts at: 2850 2850
Size: 4394
PfoI T'CCnGG_A
Cuts at: 673 673
Size: 4394
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PshAI GACnn'nnGTC
Cuts at: 1297 1297
Size: 4394
PstI C_TGCA'G
Cuts at: 1441 2126 1441
Size: 685 3709
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 242 1408 242
Size: 1166 3228
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 889 2417 2627 889
Size: 1528 210 2656
Fragments arranged by size:
2656 1528 210
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1437 2122 3115 3306 33
Size: 1404 685 993 191 1121
Fragments arranged by size:
1404 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1416 1416
Size: 4394
SmlI C'TyrA_G
Cuts at: 975 1000 1549 2464 2956 3218 975
Size: 25 549 915 492 262 2151
Fragments arranged by size:
2151 915 549 492 262 25
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StyI C'CwwG_G
Cuts at: 107 477 908 1528 1882 107
Size: 370 431 620 354 2619
Fragments arranged by size:
2619 620 431 370 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1287 1977 2110 2569 2778 1287
Size: 690 133 459 209 2903
Fragments arranged by size:
2903 690 459 209 133
TatI w'GTAC_w
Cuts at: 370 3909 370
Size: 3539 855
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 2191 2191
Size: 4394
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1889 1889
Size: 4394
XmaI C'CCGG_G
Cuts at: 1414 1414
Size: 4394
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BsaXI BseRI BsgI BsiWI BsmBI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI