(Circular) (Six-base) MAPSORT of: pF1KB6678.seq Check: 925 from: 1 to: 4421 pF1KB6678 4421 bp With 182 enzymes: SgfI * October 7, 2009 14:22 .. AccI GT'mk_AC Cuts at: 1459 1459 Size: 4421 Acc65I G'GTAC_C Cuts at: 1437 1437 Size: 4421 AclI AA'CG_TT Cuts at: 4261 4261 Size: 4421 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 162 174 445 1246 2246 2678 3425 3693 162 Size: 12 271 801 1000 432 747 268 890 Fragments arranged by size: 1000 890 801 747 432 271 268 12 AflIII A'CryG_T Cuts at: 1109 1773 2877 3854 1109 Size: 664 1104 977 1676 Fragments arranged by size: 1676 1104 977 664 AgeI A'CCGG_T Cuts at: 2809 2809 Size: 4421 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1058 1090 1058 Size: 32 4389 AlwNI CAG_nnn'CTG Cuts at: 294 529 1726 3293 294 Size: 235 1197 1567 1422 Fragments arranged by size: 1567 1422 1197 235 ApaI G_GGCC'C Cuts at: 157 157 Size: 4421 ApaLI G'TGCA_C Cuts at: 3191 3191 Size: 4421 ApoI r'AATT_y Cuts at: 1425 3774 4030 1425 Size: 2349 256 1816 Fragments arranged by size: 2349 1816 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4421 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4421 AvaI C'yCGr_G Cuts at: 150 156 201 1441 150 Size: 6 45 1240 3130 Fragments arranged by size: 3130 1240 45 6 BamHI G'GATC_C Cuts at: 615 1446 615 Size: 831 3590 BanI G'GyrC_C Cuts at: 1437 1851 2099 2134 1437 Size: 414 248 35 3724 Fragments arranged by size: 3724 414 248 35 BanII G_rGCy'C Cuts at: 157 208 1435 2465 157 Size: 51 1227 1030 2113 Fragments arranged by size: 2113 1227 1030 51 BbeI G_GCGC'C Cuts at: 1855 2103 1855 Size: 248 4173 BbsI GAAGACnn'nnnn_ Cuts at: 451 451 Size: 4421 BbvCI CC'TCA_GC Cuts at: 494 494 Size: 4421 BciVI GTATCCnnnnn_n' Cuts at: 2319 3080 4421 2319 Size: 761 1341 2319 Fragments arranged by size: 2319 1341 761 BclI T'GATC_A Cuts at: 1941 1941 Size: 4421 BglI GCCn_nnn'nGGC Cuts at: 142 142 Size: 4421 BglII A'GATC_T Cuts at: 1936 3973 1936 Size: 2037 2384 BlpI GC'TnA_GC Cuts at: 1533 1533 Size: 4421 Bme1580I G_kGCm'C Cuts at: 157 2046 2139 3195 157 Size: 1889 93 1056 1383 Fragments arranged by size: 1889 1383 1056 93 BmgBI CAC'GTC Cuts at: 1195 1195 Size: 4421 BmrI ACTGGGnnnn_n' Cuts at: 1303 1633 1714 2049 4193 1303 Size: 330 81 335 2144 1531 Fragments arranged by size: 2144 1531 335 330 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2788 2788 Size: 4421 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 192 192 Size: 4421 Bpu10I CC'TnA_GC Cuts at: 494 494 Size: 4421 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1597 2476 2968 3266 1597 Size: 879 492 298 2752 Fragments arranged by size: 2752 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 1135 1214 4125 1135 Size: 79 2911 1431 Fragments arranged by size: 2911 1431 79 BsaAI yAC'GTr Cuts at: 1774 1774 Size: 4421 BsaBI GATnn'nnATC Cuts at: 6 538 1935 3972 6 Size: 532 1397 2037 455 Fragments arranged by size: 2037 1397 532 455 BsaHI Gr'CG_yC Cuts at: 217 819 1852 2100 2802 4298 217 Size: 602 1033 248 702 1496 340 Fragments arranged by size: 1496 1033 702 602 340 248 BsaWI w'CCGG_w Cuts at: 1610 2131 2809 3083 3230 3641 1610 Size: 521 678 274 147 411 2390 Fragments arranged by size: 2390 678 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 190 220 1058 1088 190 Size: 30 838 30 3523 Fragments arranged by size: 3523 838 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1079 1079 Size: 4421 BseYI C'CCAG_C Cuts at: 1026 1375 1846 2604 3181 3677 1026 Size: 349 471 758 577 496 1770 Fragments arranged by size: 1770 758 577 496 471 349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1106 1106 Size: 4421 BsiEI CG_ry'CG Cuts at: 104 1963 2009 3217 104 Size: 1859 46 1208 1308 Fragments arranged by size: 1859 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 1435 2213 2403 3195 1435 Size: 778 190 792 2661 Fragments arranged by size: 2661 792 778 190 BsmI GAATG_Cn' Cuts at: 318 960 318 Size: 642 3779 Bsp1286I G_dGCh'C Cuts at: 157 208 1435 2046 2139 2213 2403 2465 3195 Size: 51 1227 611 93 74 190 62 730 Cuts at: 3195 157 Size: 1383 Fragments arranged by size: 1383 1227 730 611 190 93 74 62 51 BspEI T'CCGG_A Cuts at: 3641 3641 Size: 4421 BspHI T'CATG_A Cuts at: 3597 3597 Size: 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1471 1685 1987 2368 1471 Size: 214 302 381 3524 Fragments arranged by size: 3524 381 302 214 BsrBI CCG'CTC Cuts at: 50 276 2713 2767 4251 4416 50 Size: 226 2437 54 1484 165 55 Fragments arranged by size: 2437 1484 226 165 55 54 BsrDI GCAATG_nn' Cuts at: 783 965 2333 783 Size: 182 1368 2871 Fragments arranged by size: 2871 1368 182 BsrFI r'CCGG_y Cuts at: 145 172 2419 2600 2809 145 Size: 27 2247 181 209 1757 Fragments arranged by size: 2247 1757 209 181 27 BssHII G'CGCG_C Cuts at: 165 256 165 Size: 91 4330 BssSI C'ACGA_G Cuts at: 1133 1351 2692 3050 1133 Size: 218 1341 358 2504 Fragments arranged by size: 2504 1341 358 218 BstAPI GCAn_nnn'nTGC Cuts at: 354 1798 354 Size: 1444 2977 BstBI TT'CG_AA Cuts at: 2782 2782 Size: 4421 BstXI CCAn_nnnn'nTGG Cuts at: 114 114 Size: 4421 BstYI r'GATC_y Cuts at: 1 615 1446 1913 1936 2271 2517 3529 3615 Size: 614 831 467 23 335 246 1012 86 Cuts at: 3615 3627 3973 1 Size: 12 346 449 Fragments arranged by size: 1012 831 614 467 449 346 335 246 86 23 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2371 2552 2715 2371 Size: 181 163 4077 Fragments arranged by size: 4077 181 163 BtsI GCAGTG_nn' Cuts at: 1160 1416 1664 1160 Size: 256 248 3917 Fragments arranged by size: 3917 256 248 ClaI AT'CG_AT Cuts at: 654 654 Size: 4421 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 884 919 884 Size: 35 4386 DraI TTT'AAA Cuts at: 1420 1420 Size: 4421 DraIII CAC_nnn'GTG Cuts at: 936 936 Size: 4421 DrdI GACnn_nn'nnGTC Cuts at: 1369 2127 2985 1369 Size: 758 858 2805 Fragments arranged by size: 2805 858 758 EaeI y'GGCC_r Cuts at: 143 2006 2180 2571 2598 3943 143 Size: 1863 174 391 27 1345 621 Fragments arranged by size: 1863 1345 621 391 174 27 EagI C'GGCC_G Cuts at: 2006 2006 Size: 4421 EarI CTCTTCn'nnn_ Cuts at: 2444 2654 2444 Size: 210 4211 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2629 2939 3085 4231 2629 Size: 310 146 1146 2819 Fragments arranged by size: 2819 1146 310 146 EcoICRI GAG'CTC Cuts at: 1433 1433 Size: 4421 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 162 174 192 445 1246 2246 2678 3425 3693 Size: 12 18 253 801 1000 432 747 268 Cuts at: 3693 162 Size: 890 Fragments arranged by size: 1000 890 801 747 432 268 253 18 12 EcoO109I rG'GnC_Cy Cuts at: 1560 1560 Size: 4421 EcoRI G'AATT_C Cuts at: 1425 1425 Size: 4421 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 621 2440 621 Size: 1819 2602 FseI GG_CCGG'CC Cuts at: 149 149 Size: 4421 FspI TGC'GCA Cuts at: 2202 2202 Size: 4421 HaeII r_GCGC'y Cuts at: 1855 2103 3125 4104 1855 Size: 248 1022 979 2172 Fragments arranged by size: 2172 1022 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 190 222 289 321 2263 2293 2295 2325 190 Size: 32 67 32 1942 30 2 30 2286 Fragments arranged by size: 2286 1942 67 32 32 30 30 2 HincII GTy'rAC Cuts at: 1286 1460 1286 Size: 174 4247 KasI G'GCGC_C Cuts at: 1851 2099 1851 Size: 248 4173 KpnI G_GTAC'C Cuts at: 1441 1441 Size: 4421 MfeI C'AATT_G Cuts at: 430 430 Size: 4421 MluI A'CGCG_T Cuts at: 3854 3854 Size: 4421 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 3092 3276 3092 Size: 184 4237 MscI TGG'CCA Cuts at: 2182 2182 Size: 4421 MslI CAynn'nnrTG Cuts at: 557 2410 2537 557 Size: 1853 127 2441 Fragments arranged by size: 2441 1853 127 MspA1I CmG'CkG Cuts at: 1532 1846 2206 3219 3464 1532 Size: 314 360 1013 245 2489 Fragments arranged by size: 2489 1013 360 314 245 NaeI GCC'GGC Cuts at: 147 174 2602 147 Size: 27 2428 1966 Fragments arranged by size: 2428 1966 27 NarI GG'CG_CC Cuts at: 1852 2100 1852 Size: 248 4173 NgoMIV G'CCGG_C Cuts at: 145 172 2600 145 Size: 27 2428 1966 Fragments arranged by size: 2428 1966 27 NspI r_CATG'y Cuts at: 1113 1474 1974 2881 1113 Size: 361 500 907 2653 Fragments arranged by size: 2653 907 500 361 PasI CC'CwG_GG Cuts at: 579 579 Size: 4421 PciI A'CATG_T Cuts at: 1109 2877 1109 Size: 1768 2653 PfoI T'CCnGG_A Cuts at: 293 293 Size: 4421 PmeI GTTT'AAAC Cuts at: 1420 1420 Size: 4421 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 1058 1090 3560 3592 1058 Size: 32 2470 32 1887 Fragments arranged by size: 2470 1887 32 32 PspOMI G'GGCC_C Cuts at: 153 153 Size: 4421 PstI C_TGCA'G Cuts at: 1468 2153 1468 Size: 685 3736 PvuI CG_AT'CG Cuts at: 104 104 Size: 4421 PvuII CAG'CTG Cuts at: 1846 2206 1846 Size: 360 4061 RsrII CG'GwC_CG Cuts at: 2616 2616 Size: 4421 SacI G_AGCT'C Cuts at: 1435 1435 Size: 4421 SalI G'TCGA_C Cuts at: 1458 1458 Size: 4421 SapI GCTCTTCn'nnn_ Cuts at: 2444 2654 2444 Size: 210 4211 SbfI CC_TGCA'GG Cuts at: 1468 1468 Size: 4421 ScaI AGT'ACT Cuts at: 643 3938 643 Size: 3295 1126 SfcI C'TryA_G Cuts at: 33 1464 2149 3142 3333 33 Size: 1431 685 993 191 1121 Fragments arranged by size: 1431 1121 993 685 191 SfoI GGC'GCC Cuts at: 1853 2101 1853 Size: 248 4173 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4421 SmaI CCC'GGG Cuts at: 152 158 1443 152 Size: 6 1285 3130 Fragments arranged by size: 3130 1285 6 SmlI C'TyrA_G Cuts at: 1576 2491 2983 3245 1576 Size: 915 492 262 2752 Fragments arranged by size: 2752 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4421 SphI G_CATG'C Cuts at: 1474 1974 1474 Size: 500 3921 SrfI GCCC'GGGC Cuts at: 152 152 Size: 4421 StyI C'CwwG_G Cuts at: 140 1215 1555 1909 140 Size: 1075 340 354 2652 Fragments arranged by size: 2652 1075 354 340 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1076 2004 2137 2596 2805 1076 Size: 928 133 459 209 2692 Fragments arranged by size: 2692 928 459 209 133 TatI w'GTAC_w Cuts at: 641 780 3936 641 Size: 139 3156 1126 Fragments arranged by size: 3156 1126 139 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 1196 1228 3938 3970 1196 Size: 32 2710 32 1647 Fragments arranged by size: 2710 1647 32 32 Tth111I GACn'n_nGTC Cuts at: 2218 2218 Size: 4421 XbaI T'CTAG_A Cuts at: 1452 1452 Size: 4421 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1916 1916 Size: 4421 XmaI C'CCGG_G Cuts at: 150 156 1441 150 Size: 6 1285 3130 Fragments arranged by size: 3130 1285 6 XmnI GAAnn'nnTTC Cuts at: 289 289 Size: 4421 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsmBI BsrGI BstEII BstZ17I Bsu36I BtgI EcoNI EcoRV FspAI HindIII HpaI NcoI NdeI NheI NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI XhoI ZraI