Restriction map for
pF1KB6678
(Circular) (Six-base) MAPSORT of: pF1KB6678.seq Check: 925 from: 1 to: 4421
pF1KB6678 4421 bp
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AccI GT'mk_AC
Cuts at: 1459 1459
Size: 4421
Acc65I G'GTAC_C
Cuts at: 1437 1437
Size: 4421
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 174 445 1246 2246 2678 3425 3693 162
Size: 12 271 801 1000 432 747 268 890
Fragments arranged by size:
1000 890 801 747 432 271 268 12
AflIII A'CryG_T
Cuts at: 1109 1773 2877 3854 1109
Size: 664 1104 977 1676
Fragments arranged by size:
1676 1104 977 664
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1058 1090 1058
Size: 32 4389
AlwNI CAG_nnn'CTG
Cuts at: 294 529 1726 3293 294
Size: 235 1197 1567 1422
Fragments arranged by size:
1567 1422 1197 235
ApaI G_GGCC'C
Cuts at: 157 157
Size: 4421
ApaLI G'TGCA_C
Cuts at: 3191 3191
Size: 4421
ApoI r'AATT_y
Cuts at: 1425 3774 4030 1425
Size: 2349 256 1816
Fragments arranged by size:
2349 1816 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 150 156 201 1441 150
Size: 6 45 1240 3130
Fragments arranged by size:
3130 1240 45 6
BamHI G'GATC_C
Cuts at: 615 1446 615
Size: 831 3590
BanI G'GyrC_C
Cuts at: 1437 1851 2099 2134 1437
Size: 414 248 35 3724
Fragments arranged by size:
3724 414 248 35
BanII G_rGCy'C
Cuts at: 157 208 1435 2465 157
Size: 51 1227 1030 2113
Fragments arranged by size:
2113 1227 1030 51
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbsI GAAGACnn'nnnn_
Cuts at: 451 451
Size: 4421
BbvCI CC'TCA_GC
Cuts at: 494 494
Size: 4421
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 1941 1941
Size: 4421
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 4421
BglII A'GATC_T
Cuts at: 1936 3973 1936
Size: 2037 2384
BlpI GC'TnA_GC
Cuts at: 1533 1533
Size: 4421
Bme1580I G_kGCm'C
Cuts at: 157 2046 2139 3195 157
Size: 1889 93 1056 1383
Fragments arranged by size:
1889 1383 1056 93
BmgBI CAC'GTC
Cuts at: 1195 1195
Size: 4421
BmrI ACTGGGnnnn_n'
Cuts at: 1303 1633 1714 2049 4193 1303
Size: 330 81 335 2144 1531
Fragments arranged by size:
2144 1531 335 330 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 4421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 192 192
Size: 4421
Bpu10I CC'TnA_GC
Cuts at: 494 494
Size: 4421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1135 1214 4125 1135
Size: 79 2911 1431
Fragments arranged by size:
2911 1431 79
BsaAI yAC'GTr
Cuts at: 1774 1774
Size: 4421
BsaBI GATnn'nnATC
Cuts at: 6 538 1935 3972 6
Size: 532 1397 2037 455
Fragments arranged by size:
2037 1397 532 455
BsaHI Gr'CG_yC
Cuts at: 217 819 1852 2100 2802 4298 217
Size: 602 1033 248 702 1496 340
Fragments arranged by size:
1496 1033 702 602 340 248
BsaWI w'CCGG_w
Cuts at: 1610 2131 2809 3083 3230 3641 1610
Size: 521 678 274 147 411 2390
Fragments arranged by size:
2390 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 190 220 1058 1088 190
Size: 30 838 30 3523
Fragments arranged by size:
3523 838 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1079 1079
Size: 4421
BseYI C'CCAG_C
Cuts at: 1026 1375 1846 2604 3181 3677 1026
Size: 349 471 758 577 496 1770
Fragments arranged by size:
1770 758 577 496 471 349
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1106 1106
Size: 4421
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3217 104
Size: 1859 46 1208 1308
Fragments arranged by size:
1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1435 2213 2403 3195 1435
Size: 778 190 792 2661
Fragments arranged by size:
2661 792 778 190
BsmI GAATG_Cn'
Cuts at: 318 960 318
Size: 642 3779
Bsp1286I G_dGCh'C
Cuts at: 157 208 1435 2046 2139 2213 2403 2465 3195
Size: 51 1227 611 93 74 190 62 730
Cuts at: 3195 157
Size: 1383
Fragments arranged by size:
1383 1227 730 611 190 93 74 62
51
BspEI T'CCGG_A
Cuts at: 3641 3641
Size: 4421
BspHI T'CATG_A
Cuts at: 3597 3597
Size: 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1471 1685 1987 2368 1471
Size: 214 302 381 3524
Fragments arranged by size:
3524 381 302 214
BsrBI CCG'CTC
Cuts at: 50 276 2713 2767 4251 4416 50
Size: 226 2437 54 1484 165 55
Fragments arranged by size:
2437 1484 226 165 55 54
BsrDI GCAATG_nn'
Cuts at: 783 965 2333 783
Size: 182 1368 2871
Fragments arranged by size:
2871 1368 182
BsrFI r'CCGG_y
Cuts at: 145 172 2419 2600 2809 145
Size: 27 2247 181 209 1757
Fragments arranged by size:
2247 1757 209 181 27
BssHII G'CGCG_C
Cuts at: 165 256 165
Size: 91 4330
BssSI C'ACGA_G
Cuts at: 1133 1351 2692 3050 1133
Size: 218 1341 358 2504
Fragments arranged by size:
2504 1341 358 218
BstAPI GCAn_nnn'nTGC
Cuts at: 354 1798 354
Size: 1444 2977
BstBI TT'CG_AA
Cuts at: 2782 2782
Size: 4421
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4421
BstYI r'GATC_y
Cuts at: 1 615 1446 1913 1936 2271 2517 3529 3615
Size: 614 831 467 23 335 246 1012 86
Cuts at: 3615 3627 3973 1
Size: 12 346 449
Fragments arranged by size:
1012 831 614 467 449 346 335 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 1160 1416 1664 1160
Size: 256 248 3917
Fragments arranged by size:
3917 256 248
ClaI AT'CG_AT
Cuts at: 654 654
Size: 4421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 884 919 884
Size: 35 4386
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DraIII CAC_nnn'GTG
Cuts at: 936 936
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 1369 2127 2985 1369
Size: 758 858 2805
Fragments arranged by size:
2805 858 758
EaeI y'GGCC_r
Cuts at: 143 2006 2180 2571 2598 3943 143
Size: 1863 174 391 27 1345 621
Fragments arranged by size:
1863 1345 621 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 2939 3085 4231 2629
Size: 310 146 1146 2819
Fragments arranged by size:
2819 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 162 174 192 445 1246 2246 2678 3425 3693
Size: 12 18 253 801 1000 432 747 268
Cuts at: 3693 162
Size: 890
Fragments arranged by size:
1000 890 801 747 432 268 253 18
12
EcoO109I rG'GnC_Cy
Cuts at: 1560 1560
Size: 4421
EcoRI G'AATT_C
Cuts at: 1425 1425
Size: 4421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 621 2440 621
Size: 1819 2602
FseI GG_CCGG'CC
Cuts at: 149 149
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 1855 2103 3125 4104 1855
Size: 248 1022 979 2172
Fragments arranged by size:
2172 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 190 222 289 321 2263 2293 2295 2325 190
Size: 32 67 32 1942 30 2 30 2286
Fragments arranged by size:
2286 1942 67 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1286 1460 1286
Size: 174 4247
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1441 1441
Size: 4421
MfeI C'AATT_G
Cuts at: 430 430
Size: 4421
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3092 3276 3092
Size: 184 4237
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 557 2410 2537 557
Size: 1853 127 2441
Fragments arranged by size:
2441 1853 127
MspA1I CmG'CkG
Cuts at: 1532 1846 2206 3219 3464 1532
Size: 314 360 1013 245 2489
Fragments arranged by size:
2489 1013 360 314 245
NaeI GCC'GGC
Cuts at: 147 174 2602 147
Size: 27 2428 1966
Fragments arranged by size:
2428 1966 27
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NgoMIV G'CCGG_C
Cuts at: 145 172 2600 145
Size: 27 2428 1966
Fragments arranged by size:
2428 1966 27
NspI r_CATG'y
Cuts at: 1113 1474 1974 2881 1113
Size: 361 500 907 2653
Fragments arranged by size:
2653 907 500 361
PasI CC'CwG_GG
Cuts at: 579 579
Size: 4421
PciI A'CATG_T
Cuts at: 1109 2877 1109
Size: 1768 2653
PfoI T'CCnGG_A
Cuts at: 293 293
Size: 4421
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1058 1090 3560 3592 1058
Size: 32 2470 32 1887
Fragments arranged by size:
2470 1887 32 32
PspOMI G'GGCC_C
Cuts at: 153 153
Size: 4421
PstI C_TGCA'G
Cuts at: 1468 2153 1468
Size: 685 3736
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1846 2206 1846
Size: 360 4061
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 643 3938 643
Size: 3295 1126
SfcI C'TryA_G
Cuts at: 33 1464 2149 3142 3333 33
Size: 1431 685 993 191 1121
Fragments arranged by size:
1431 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SmaI CCC'GGG
Cuts at: 152 158 1443 152
Size: 6 1285 3130
Fragments arranged by size:
3130 1285 6
SmlI C'TyrA_G
Cuts at: 1576 2491 2983 3245 1576
Size: 915 492 262 2752
Fragments arranged by size:
2752 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
SrfI GCCC'GGGC
Cuts at: 152 152
Size: 4421
StyI C'CwwG_G
Cuts at: 140 1215 1555 1909 140
Size: 1075 340 354 2652
Fragments arranged by size:
2652 1075 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1076 2004 2137 2596 2805 1076
Size: 928 133 459 209 2692
Fragments arranged by size:
2692 928 459 209 133
TatI w'GTAC_w
Cuts at: 641 780 3936 641
Size: 139 3156 1126
Fragments arranged by size:
3156 1126 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1196 1228 3938 3970 1196
Size: 32 2710 32 1647
Fragments arranged by size:
2710 1647 32 32
Tth111I GACn'n_nGTC
Cuts at: 2218 2218
Size: 4421
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1916 1916
Size: 4421
XmaI C'CCGG_G
Cuts at: 150 156 1441 150
Size: 6 1285 3130
Fragments arranged by size:
3130 1285 6
XmnI GAAnn'nnTTC
Cuts at: 289 289
Size: 4421
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PciI PfoI PmeI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AscI
AvrII BaeI BcgI BfrBI BmtI BsiWI BsmBI BsrGI
BstEII BstZ17I Bsu36I BtgI EcoNI EcoRV FspAI HindIII
HpaI NcoI NdeI NheI NotI NruI NsiI PacI
PflMI PmlI PpuMI PshAI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SspI StuI SwaI
XhoI ZraI